ragtag: ragtag.xml comparison

comparison ragtag.xml @ 0:a04e64efa43a draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ragtag commit 4c4b2a548b4ce46da88810992459b3ac8581d035"

author	iuc
date	Wed, 10 Nov 2021 23:33:13 +0000
parents
children	d110a4141898

comparison

equal deleted inserted replaced

--1:000000000000
+:a04e64efa43a
+<tool id='ragtag' name='RagTag' version='@TOOL_VERSION@+galaxy@VERSION_SUFFIX@' profile='20.01'>
+<description>reference-guided scaffolding of draft genomes</description>
+<macros>
+<import>macros.xml</import>
+</macros>
+<expand macro='xrefs' />
+<expand macro='requirements' />
+<command detect_errors='exit_code'><![CDATA[
+#if $mode_conditional.mode_option != 'merge'
+#if $mode_conditional.advanced_options.mapping_conditional.mapping_option == 'nucmer'
+#set $nucmer_params = '%s -l %s -c %s' % ($mode_conditional.advanced_options.mapping_conditional.anchor_mode,
+$mode_conditional.advanced_options.mapping_conditional.l,
+$mode_conditional.advanced_options.mapping_conditional.c)
+#end if
+#end if
+#if $mode_conditional.mode_option == 'merge'
+#set $input_files = list()
+mkdir merge_files &&
+#for $i, $j in enumerate($mode_conditional.scaffold_files)
+#set $out_file = './merge_files/scaffold_%s.agp' % $i
+ln -s '${j}' $out_file &&
+$input_files.append($out_file)
+#end for
+#set $merge_files = " ".join($input_files)
+#end if
+ragtag.py $mode_conditional.mode_option -u
+#if $mode_conditional.mode_option == 'correct'
+@INPUTS@
+@COMMON_PARAMETERS@
+#if $mode_conditional.validation_conditional.validation_option == 'true'
+-R '${mode_conditional.validation_conditional.R}'
+-T $mode_conditional.validation_conditional.read_type
+-v $mode_conditional.validation_conditional.v
+#if $mode_conditional.validation_conditional.max_cov
+--max-cov $mode_conditional.validation_conditional.max_cov
+#end if
+#if $mode_conditional.validation_conditional.min_cov
+--min-cov $mode_conditional.validation_conditional.min_cov
+#end if
+#end if
+-b $mode_conditional.advanced_options.b
+#if $mode_conditional.advanced_options.missasembly_break
+$mode_conditional.advanced_options.missasembly_break
+#end if
+#if $mode_conditional.advanced_options.gff
+--gff '${mode_conditional.advanced_options.gff}'
+#end if
+--read-aligner 'minimap2' ## it is the only allowed
+#else if $mode_conditional.mode_option == 'scaffold'
+@INPUTS@
+@COMMON_PARAMETERS@
+-i $mode_conditional.advanced_options.i
+-a $mode_conditional.advanced_options.a
+-s $mode_conditional.advanced_options.s
+#if $mode_conditional.advanced_options.gap_conditional.gap_option == 'true'
+-r
+-g '${mode_conditional.advanced_options.gap_conditional.g}'
+-m '${mode_conditional.advanced_options.gap_conditional.m}'
+#end if
+#if $mode_conditional.advanced_options.unplaced_conditional.unplaced_option == 'true'
+-C
+#if $mode_conditional.advanced_options.unplaced_conditional.J
+-J '${mode_conditional.advanced_options.unplaced_conditional.J}'
+#end if
+#end if
+#else if $mode_conditional.mode_option == 'patch'
+@INPUTS@
+@COMMON_PARAMETERS@
+-s $mode_conditional.advanced_options.s
+-i $mode_conditional.advanced_options.i
+#if $mode_conditional.advanced_options.patching_mode
+$mode_conditional.advanced_options.patching_mode
+#end if
+#else
+$assembly_fasta
+#if $mode_conditional.scaffold_files
+$merge_files
+#end if
+#if $mode_conditional.merging_options.j
+-j $mode_conditional.merging_options.j
+#end if
+-l $mode_conditional.merging_options.l
+-e $mode_conditional.merging_options.e
+--gap-func $mode_conditional.merging_options.function_merging
+#if $mode_conditional.hic_options.b
+-b $mode_conditional.hic_options.b
+-r $mode_conditional.hic_options.r
+-p $mode_conditional.hic_options.p
+#end if
+#end if
+-o ./
+#if $mode_conditional.mode_option != 'merge'
+-t \${GALAXY_SLOTS:-2}
+#end if
+#if $mode_conditional.mode_option == 'patch'
+&& mv ragtag.patch.asm.paf.log ragtag.patch.log
+#end if
+]]>    </command>
+<inputs>
+<conditional name="mode_conditional">
+<param name="mode_option" type="select" label="Operation mode">
+<option value="correct">Correct: homology-based missasembly correction</option>
+<option value="scaffold">Scaffold: homology-based assebly scaffolding</option>
+<option value="patch">Patch: homology-based assembly patching</option>
+<option value="merge">Merge: scaffolding merging</option>
+</param>
+<when value="correct">
+<expand macro="input_options"/>
+<conditional name="validation_conditional">
+<param name="validation_option" type="select" label="Use validation reads">
+<option value="true">Enabled</option>
+<option value="false" selected="true">Disabled</option>
+</param>
+<when value="true">
+<param argument="-R" type="data" format="fastq,fastqsanger" label="Validation reads"
+help="Without validation, the module will break at any point of reference discordance as defined by the 'correction options'.
+With validation, RagTag maps reads to the query assembly and verifies putative break points if they are near regions of
+exceptionally low or high coverage. The reads used for validation should come from the same genotype as the query
+assembly to ensure that coverage abnormalities don't arise from true biological variation" />
+<param name="read_type" type="select" label="Read type">
+<option value="sr">Illumina</option>
+<option value="ont">Nanopore</option>
+<option value="corr">Error corrected long-reads</option>
+</param>
+<param argument="-v" type="integer" min="0" value="10000" label="Coverage validation window size"
+help="This parameter specifies the window around the putative misassembly break point that RagTag examines
+for exceptionally low or high read coverage. The larger this window size, the more likely
+it is to find an unrelated coverage abnormality"/>
+<param argument="--max-cov" type="integer" min="0" value="" optional="true" label="Break sequences at regions at or above this coverage level"/>
+<param argument="--min-cov" type="integer" min="0" value="" optional="true" label="Break sequences at regions at or below this coverage level"/>
+</when>
+<when value="false"/>
+</conditional>
+<section name="advanced_options" title="Advanced options">
+<expand macro="common_parameters"/>
+<param argument="-b" type="integer" min="0" value="5000" label="Minimum break distance from contig ends"
+help="Breaks will not be made within -b bp of query sequence termini"/>
+<param name="missasembly_break" type="select" optional="true" label="Break misassebly option"
+help="One can also direct RagTag to only break misassemblies between (--inter, query maps to >1 reference sequence) or within
+(--intra, query maps discordantly to 1 reference sequence) reference sequences">
+<option value="--inter">Only break misassemblies between reference sequences (--inter)</option>
+<option value="--intra">Only break missasemblies within reference sequences (--intra)</option>
+</param>
+<param argument="--gff" type="data" format="gff" optional="true" label="Don't break sequences within GFF intervals"
+help=" If one has annotations associated with the query assembly, provide them with the --gff option to ensure that the query assembly
+is never broken within annotation intervals. "/>
+</section>
+<param name="output_correct" type="select"  multiple="true" label="Output files">
+<option value="fasta" selected="true">The corrected query assembly in FASTA format</option>
+<option value="agp" selected="true">The AGP file defining the exact coordinates of query sequence breaks</option>
+<option value="paf">The description of the approximate mapping positions between two set of sequences in PAF format</option>
+<option value="log">Log file</option>
+</param>
+</when>
+<when value="scaffold">
+<expand macro="input_options"/>
+<section name="advanced_options" title="advanced options">
+<expand macro="common_parameters"/>
+<param argument="-i" type="float" min="0" max="1" value="0.2" label="Minimum grouping confidence score"
+help="The grouping confidence score is the number of base pairs a contig covered in its assigned reference chromosome
+divided by the total number of covered base pairs in the entire reference genome"/>
+<param argument="-a" type="float" min="0" max="1" value="0" label="Minimum location confidence score"
+help="To create a metric associated with contig ordering confidence, Ragtag define a location confidence. First, the smallest
+and largest alignment positions, with respect to the reference, between a contig and its assigned reference chromosome are found.
+The location confidence is then calculated as the number of covered base pairs in this range divided by the total number of
+base pairs in the range"/>
+<param argument="-s" type="float" min="0" max="1" value="0" label="Minimum orientation confidence score"
+help="To calculate the orientation confidence, each base pair in each alignment between a contig and its assigned reference chromosome
+casts a vote for the orientation of its alignment. The orientation confidence is the number of votes for the assigned orientation of
+the contig divided by the total number of votes"/>
+<conditional name="gap_conditional">
+<param name="gap_option" type="select" label="Infer gap sizes" help="When disabled, all gaps are 100 bp (-r)">
+<option value="true" selected="true">Enabled</option>
+<option value="false">Disabled</option>
+</param>
+<when value="true">
+<param argument="-g" type="integer" min="0" value="100" label="Minimum infered gap size" />
+<param argument="-m" type="integer" min="0" value="100000" label="Maximum inferred gap size"/>
+</when>
+<when value="false"/>
+</conditional>
+<conditional name="unplaced_conditional">
+<param name="unplaced_option" type="select" label="Concatenate unplaced contigs and make 'chr0' (-C)">
+<option value="true">Enabled</option>
+<option value="false" selected="true">Disabled</option>
+</param>
+<when value="true">
+<param argument="-J" type="data" format="txt" optional="true" label="List of query headers to leave unplaceds and exclude form 'chr0'"/>
+</when>
+<when value="false"/>
+</conditional>
+</section>
+<param name="output_scaffold" type="select"  multiple="true" label="Output files">
+<option value="fasta" selected="true">The scaffolds in FASTA format, defined by the ordering and orientations of the sequences containted in the AGP file</option>
+<option value="agp" selected="true">The ordering and orientations of query sequences in AGP format</option>
+<option value="paf">The description of the approximate mapping positions between two set of sequences in PAF format</option>
+<option value="confidence">Confidence score values</option>
+<option value="stats">Summary statistics for the scaffolding process</option>
+<option value="log">Log file</option>
+</param>
+</when>
+<when value="patch">
+<expand macro="input_options"/>
+<section name="advanced_options" title="advanced options">
+<expand macro="common_parameters"/>
+<param argument="-s" type="integer" min="0" value="50000" label="Minimum merged alignment length"
+help="After merging, alignments less than -s bp long will be removed"/>
+<param argument="-i" type="float" min="0" max="1" value="0.05" label="Maximum merged alignment distance"
+help="Maximum merged alignment distance from sequence terminus as fraction of the sequence length. Alignments must
+be within -i bp of a target sequence terminus or gap to be considered for patchin "/>
+<param name="patching_mode" type="select" optional="true" label="Patching mode">
+<option value="--fill-only">Only fill existing target gaps. Do not join target sequences</option>
+<option value="--join_only">Only join and patch target sequences. DO not fill existing gaps</option>
+</param>
+</section>
+<param name="output_patch" type="select"  multiple="true" label="Output files">
+<option value="final_fasta" selected="true">The final FASTA file containing the patched assembly</option>
+<option value="final_agp" selected="true">The final AGP file defining how final FASTA is built</option>
+<option value="assembly_file" selected="true">Assembly alignment files</option>
+<option value="split_assembly">The split target assembly and the renamed query assembly combined into one FASTA file</option>
+<option value="split_description">An AGP file defining how the target assembly was split at gaps</option>
+<option value="target_gaps">The target assembly split at gaps</option>
+<option value="agp_renamed">An AGP file defining the new names for query sequences</option>
+<option value="fasta_renamed">A FASTA file with the original query sequence, but with new names</option>
+<option value="log">Log file</option>
+</param>
+</when>
+<when value="merge">
+<param name="assembly_fasta" type="data" format="fasta" label="Assembly FASTA file"/>
+<param name="scaffold_files" type="data" format="agp" multiple="true" optional="true" label="Scaffold AGP files"/>
+<section name="merging_options" title="Merging options">
+<param argument="-j" type="data" format="txt" optional="true" label="List of query headers to leave unplaced"/>
+<param argument="-l" type="integer" min="0" value="100000" label="Minimum assembly sequence length"
+help="Assembly sequences shorter than -l will also be left unplaced."/>
+<param argument="-e" type="float" min="0" value="0" label="Minimum edge weight"
+help="The edges in the merging graph represent scaffolding adjacencies. If an AGP file supports a particular adjacency,
+its weight is added to the edge weight. Any edges with a weight lower than the minimum edge weigth will be removed from the graph"/>
+<param name="function_merging" type="select" label="Function for merging gap lengths"
+help="Scaffold gaps can differ between input AGP files. For example, a Hi-C derived AGP file might place 100 bp gaps between sequences
+while a reference-guided AGP file might infer gap sizes based on a reference genome. Use this parameter to specify how gap sizes
+should be computed from the supporting AGP files (--gap-func)">
+<option value="min" selected="true">Min</option>
+<option value="max">Max</option>
+<option value="mean">Mean</option>
+</param>
+</section>
+<section name="hic_options" title="HI-C options">
+<param argument="-b" type="data" format="bam" optional="true" label="Hi-C alignments" help="Sorted by read name"/>
+<param argument="-r" type="text" value="" optional="true" label="Restriction enzymes/sites or 'DNase'" help="List of restrction enzimes/sites or 'DNase', separated by comma. E.g. GATC,GACC">
+<sanitizer invalid_char="">
+<valid initial="string.letters,string.digits">
+<add value="," />
+<add value="[" />
+<add value="]" />
+</valid>
+</sanitizer>
+<validator type="regex">[0-9a-zA-Z,\]\[]+</validator>
+</param>
+<param argument="-p" type="float" min="0" max="1" value="1" optional="true" label="Portion of the sequence termini to consider for links"/>
+</section>
+</when>
+</conditional>
+</inputs>
+<outputs>
+<!--Correct mode outputs-->
+<data format="paf" name="correct_paf" from_work_dir="ragtag.correct.asm.paf" label="${tool.name} on ${on_string}: PAF">
+<filter>mode_conditional["mode_option"] == "correct" and "paf" in mode_conditional["output_correct"]</filter>
+</data>
+<data format="agp" name="correct_agp" from_work_dir="ragtag.correct.agp" label="${tool.name} on ${on_string}: AGP">
+<filter>mode_conditional["mode_option"] == "correct" and "agp" in mode_conditional["output_correct"]</filter>
+</data>
+<data format="fasta" name="correct_fasta" from_work_dir="ragtag.correct.fasta" label="${tool.name} on ${on_string}: FASTA">
+<filter>mode_conditional["mode_option"] == "correct" and "fasta" in mode_conditional["output_correct"]</filter>
+</data>
+<data format="txt" name="correct_log" from_work_dir="ragtag.correct.asm.paf.log" label="${tool.name} on ${on_string}: log">
+<filter>mode_conditional["mode_option"] == "correct" and "log" in mode_conditional["output_correct"]</filter>
+</data>
+<!--Scaffold mode outputs-->
+<data format="paf" name="scaffold_paf" from_work_dir="ragtag.scaffold.asm.paf" label="${tool.name} on ${on_string}: PAF">
+<filter>mode_conditional["mode_option"] == "scaffold" and "paf" in mode_conditional["output_scaffold"]</filter>
+</data>
+<data format="agp" name="scaffold_agp" from_work_dir="ragtag.scaffold.agp" label="${tool.name} on ${on_string}: AGP">
+<filter>mode_conditional["mode_option"] == "scaffold" and "agp" in mode_conditional["output_scaffold"]</filter>
+</data>
+<data format="fasta" name="scaffold_fasta" from_work_dir="ragtag.scaffold.fasta" label="${tool.name} on ${on_string}: FASTA">
+<filter>mode_conditional["mode_option"] == "scaffold" and "fasta" in mode_conditional["output_scaffold"]</filter>
+</data>
+<data format="txt" name="scaffold_log" from_work_dir="ragtag.scaffold.asm.paf.log" label="${tool.name} on ${on_string}: log">
+<filter>mode_conditional["mode_option"] == "scaffold" and "log" in mode_conditional["output_scaffold"]</filter>
+</data>
+<data format="tabular" name="scaffold_stats" from_work_dir="ragtag.scaffold.stats" label="${tool.name} on ${on_string}: stats">
+<filter>mode_conditional["mode_option"] == "scaffold" and "stats" in mode_conditional["output_scaffold"]</filter>
+</data>
+<data format="tabular" name="scaffold_confidence" from_work_dir="ragtag.scaffold.confidence.txt" label="${tool.name} on ${on_string}: confidence">
+<filter>mode_conditional["mode_option"] == "scaffold" and "confidence" in mode_conditional["output_scaffold"]</filter>
+</data>
+<!--Patch mode outputs-->
+<data format="agp" name="patch_agp" from_work_dir="ragtag.patch.agp" label="${tool.name} on ${on_string}: final AGP">
+<filter>mode_conditional["mode_option"] == "patch" and "final_agp" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="paf" name="patch_paf" from_work_dir="ragtag.patch.asm.paf" label="${tool.name} on ${on_string}: final PAF">
+<filter>mode_conditional["mode_option"] == "patch" and "assembly_file" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="txt" name="patch_log" from_work_dir="ragtag.patch.log" label="${tool.name} on ${on_string}: log">
+<filter>mode_conditional["mode_option"] == "patch" and "log" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="fasta" name="patch_comps_fasta" from_work_dir="ragtag.patch.comps.fasta" label="${tool.name} on ${on_string}: components FASTA">
+<filter>mode_conditional["mode_option"] == "patch" and "split_assembly" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="agp" name="patch_ctg_agp" from_work_dir="ragtag.patch.ctg.agp" label="${tool.name} on ${on_string}: contigs AGP">
+<filter>mode_conditional["mode_option"] == "patch" and "split_description" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="fasta" name="patch_ctg_fasta" from_work_dir="ragtag.patch.ctg.fasta" label="${tool.name} on ${on_string}: contigs FASTA">
+<filter>mode_conditional["mode_option"] == "patch" and "target_gaps" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="fasta" name="patch_fasta" from_work_dir="ragtag.patch.fasta" label="${tool.name} on ${on_string}: final FASTA">
+<filter>mode_conditional["mode_option"] == "patch" and "final_fasta" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="agp" name="patch_rename_agp" from_work_dir="ragtag.patch.rename.agp" label="${tool.name} on ${on_string}: renamed AGP">
+<filter>mode_conditional["mode_option"] == "patch" and "agp_renamed" in mode_conditional["output_patch"]</filter>
+</data>
+<data format="fasta" name="patch_rename_fasta" from_work_dir="ragtag.patch.rename.fasta" label="${tool.name} on ${on_string}: renamed FASTA">
+<filter>mode_conditional["mode_option"] == "patch" and "fasta_renamed" in mode_conditional["output_patch"]</filter>
+</data>
+<!-- Merge mode outputs-->
+<data format="agp" name="merge_agp" from_work_dir="ragtag.merge.agp" label="${tool.name} on ${on_string}: merged AGP">
+<filter>mode_conditional["mode_option"] == "merge"</filter>
+</data>
+<data format="fasta" name="merge_fasta" from_work_dir="ragtag.merge.fasta" label="${tool.name} on ${on_string}: merged FASTA">
+<filter>mode_conditional["mode_option"] == "merge"</filter>
+</data>
+</outputs>
+<tests>
+<test expect_num_outputs="4">
+<!--Test 01 correct mode minimap2-->
+<conditional name="mode_conditional">
+<param name="mode_option" value="correct"/>
+<param name="reference" value="genome.fna"/>
+<param name="query" value="contigs.fna"/>
+<param name="output_correct" value="fasta,agp,paf,log"/>
+<section name="advanced_options">
+<param name="e" value="reference_headers_skip.txt"/>
+<param name="j" value="query_headers_skip.txt"/>
+<param name="f" value="1000"/>
+<conditional name="mapping_conditional">
+<param name="mapping_option" value="minimap2"/>
+<param name="mm2_params" value="asm5"/>
+</conditional>
+<param name="remove_small" value="false"/>
+<param name="q" value="10"/>
+<param name="d" value="100000"/>
+<param name="b" value="5000"/>
+<param name="missasembly_break" value="--inter"/>
+<param name="gff" value="annotation.gff"/>
+</section>
+</conditional>
+<output name="correct_paf" file="correct_paf_01.paf" ftype="paf"/>
+<output name="correct_agp" file="correct_agp_01.agp" ftype="agp"/>
+<output name="correct_fasta" file="correct_fasta_01.fasta" ftype="fasta"/>
+<output name="correct_log" file="correct_log_01.txt" ftype="txt" lines_diff="20"/>
+</test>
+<!--Test 02 correct mode nucmer-->
+<test expect_num_outputs="2">
+<conditional name="mode_conditional">
+<param name="mode_option" value="correct"/>
+<param name="reference" value="genome.fna"/>
+<param name="query" value="contigs.fna"/>
+<param name="output_correct" value="fasta,agp"/>
+<section name="advanced_options">
+<param name="f" value="1000"/>
+<conditional name="mapping_conditional">
+<param name="mapping_option" value="nucmer"/>
+</conditional>
+<param name="remove_small" value="true"/>
+<param name="q" value="10"/>
+<param name="d" value="100000"/>
+<param name="b" value="5000"/>
+<param name="missasembly_break" value="--inter"/>
+</section>
+</conditional>
+<output name="correct_fasta" file="correct_fasta_02.fasta" ftype="fasta"/>
+<output name="correct_agp" file="correct_agp_02.agp" ftype="agp"/>
+</test>
+<!--Test 03 scaffold mode-->
+<test expect_num_outputs="6">
+<conditional name="mode_conditional">
+<param name="mode_option" value="scaffold"/>
+<param name="reference" value="genome.fna"/>
+<param name="query" value="contigs.fna"/>
+<param name="output_scaffold" value="fasta,agp,paf,confidence,log,stats"/>
+<section name="advanced_options">
+<param name="f" value="1000"/>
+<param name="remove_small" value="true"/>
+<param name="q" value="10"/>
+<param name="d" value="100000"/>
+<param name="i" value="0.2"/>
+<param name="a" value="0"/>
+<param name="s" value="0"/>
+</section>
+</conditional>
+<output name="scaffold_paf" file="scaffold_paf_03.paf" ftype="paf"/>
+<output name="scaffold_agp" file="scaffold_apg.03.agp" ftype="agp"/>
+<output name="scaffold_fasta" file="scaffold_fasta_03.fasta" ftype="fasta"/>
+<output name="scaffold_log" file="scaffold_log_03.txt" ftype="txt" lines_diff="20"/>
+<output name="scaffold_stats" file="scaffold_stats_03.tabular" ftype="tabular"/>
+<output name="scaffold_confidence" file="scaffold_confidence_03.tabular" ftype="tabular"/>
+</test>
+<!--Test 04 patch mode-->
+<test expect_num_outputs="9">
+<conditional name="mode_conditional">
+<param name="mode_option" value="patch"/>
+<param name="reference" value="genome.fna"/>
+<param name="query" value="contigs.fna"/>
+<param name="output_patch" value="final_fasta,final_agp,assembly_file,split_assembly,split_description,target_gaps,agp_renamed,fasta_renamed,log"/>
+<section name="advanced_options">
+<param name="s" value="50000"/>
+<param name="i" value="0.05"/>
+</section>
+</conditional>
+<output name="patch_agp" file="patch_agp_04.agp" ftype="agp"/>
+<output name="patch_paf" file="patch_paf_04.paf" ftype="paf"/>
+<output name="patch_log" file="patch_log_04.txt" ftype="txt" lines_diff="20"/>
+<output name="patch_comps_fasta" ftype="fasta">
+<assert_contents>
+<has_size value="603691" delta="100" />
+</assert_contents>
+</output>
+<output name="patch_ctg_fasta" file="patch_ctg_fasta_04.fasta" ftype="fasta"/>
+<output name="patch_ctg_agp" file="patch_ctg_fasta_04.agp" ftype="agp"/>
+<output name="patch_fasta" file="patch_fasta_04.fasta" ftype="fasta"/>
+<output name="patch_rename_agp" file="patch_rename_agp.agp" ftype="agp"/>
+<output name="patch_rename_fasta" file="patch_rename_fasta.fasta" ftype="fasta"/>
+</test>
+<test expect_num_outputs="2">
+<!-- Test 05 merge mode-->
+<conditional name="mode_conditional">
+<param name="mode_option" value="merge"/>
+<param name="assembly_fasta" value="correct_fasta_01.fasta"/>
+<param name="scaffold_files" value="correct_agp_01.agp,correct_agp_02.agp"/>
+<section name="merging_options">
+<param name="l" value="100000"/>
+<param name="e" value="0"/>
+<param name="function_merging" value="min"/>
+</section>
+</conditional>
+<output name="merge_agp" file="merge_agp_05.agp" ftype="agp"/>
+<output name="merge_fasta" file="merge_fasta_05.fasta" ftype="fasta"/>
+</test>
+</tests>
+<help><![CDATA[
+.. class:: infomark
+**Purpose**
+RagTag is a collection of software tools for scaffolding and improving modern genome assemblies. Tasks include:
+- Homology-based misassembly correction
+- Homology-based assembly scaffolding and patching
+- Scaffold merging
+----
+.. class:: infomark
+**Correct mode**
+RagTag offers a correction module that uses a reference genome to identify and correct potential misassemblies in a query assembly.
+RagTag also provides the option to verify putative misassemblies by aligning reads (from the same genotype) to the query assembly
+and observing read coverage near misassembly break points. In all cases, sequence is never added or subtracted. Query sequences
+are only broken at points of putative misassembly.
+*Misassemblies vs true variation*
+Reference-guided misassembly signatures are sometimes caused by true biological structural variation if the reference and query assemblies
+represent distinct genotypes (or haplotypes). The read validation feature should help to avoid some of these misassembly false positives,
+and the validation sensitivity can be tuned with command line parameters. However, it is ultimately up to the discretion of the user to decide
+if misassembly correction is appropriate. One should validate all RagTag results with independent data (usually physical, optical, or genetic
+maps), when possible.
+----
+.. class:: infomark
+**Scaffold mode**
+Scaffolding is the process of ordering and orienting draft assembly (query) sequences into longer sequences. Gaps (stretches of "N" characters)
+are placed between adjacent query sequences to indicate the presence of unknown sequence. RagTag uses whole-genome alignments to a reference
+assembly to scaffold query sequences. RagTag does not alter input query sequence in any way and only orders and orients sequences, joining them with gaps.
+----
+.. class:: infomark
+**Patch mode**
+This mode uses one genome assembly to *patch* another genome assembly. We define two types of patches:
+- Fills are patches that fill assembly gaps. This process is like traditional gap-filling, though it uses an assembly instead of WGS sequencing reads.
+- Joins are patches that join distinct contigs. This is essentially scaffolding and gap-filling in a single step.
+----
+.. class:: infomark
+**Merge mode**
+Draft genome assemblies are often scaffolded multiple times using different approaches. For example, one might scaffold an assembly using different genome
+maps (physical, linkage, Hi-C, etc.), different methods, or different method parameters. RagTag merge is a tool to merge and reconcile different scaffoldings
+of the same assembly. In this way, one can leverage the advantages of multiple techniques to synergistically improve scaffolding.
+Most tools write scaffolding results in the AGP file format, which encodes adjacency and gap information in a plain text file. To run RagTag merge,
+one must supply the assembly in FASTA format and at least two AGP files that define a scaffolding of the assembly. Each AGP file can optionally be
+assigned a weight, allowing users to assign the relative influence of each AGP on the final result.
+If available, users can supply Hi-C alignments to the draft assembly to resolve conflicts in the merging graph. In this scenario, the input AGP
+files are used to build the initial graph, but then Hi-C alignments are used to re-weight the graph before computing the scaffolding solution.
+**List of accepted restriction enzymes**
+List of all accepted restriction enzymes and their restriction sites:
+- HindIII: AAGCTT
+- Sau3AI: GATC
+- MboI: GATC
+- DpnII: GATC
+- HinfI: GA[ATCG]TC
+- DdeI: CT[ATCG]AG
+- MseI: TTAA
+For RagTag, use a comma separated list of enzymes or sites (or a mix). For example:
+- Arima Hi-C v1.0: *Sau3AI,HinfI* or *GATC,GA[ATCG]TC*
+- Arima Hi-C v2.0: *Sau3AI,HinfI,DdeI,MseI* or *GATC,GA[ATCG]TC,CT[ATCG]AG,TTAA*
+Note that for restriction sites, wildcards are represented with python regex syntax, not IUPAC ambiguity codes. e.g. '[ATCG]' instead of 'N'.
+Restriction enzymes are not necessarily the enzyme used for sample prep. Each is only a enzyme that cuts at the corresponding restriction site.
+]]>    </help>
+<expand macro="citations" />
+</tool>

Mercurial > repos > iuc > ragtag

comparison ragtag.xml @ 0:a04e64efa43a draft