Mercurial > repos > iuc > rgrnastar
comparison rg_rnaStar.xml @ 6:2055c2667eb3 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/rgrnastar commit 427913231c5894bde2b2ed428da2b8aee30bc311
author | iuc |
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date | Thu, 01 Mar 2018 11:56:28 -0500 |
parents | 99b17b74a8cd |
children | a70a7eebb435 |
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5:99b17b74a8cd | 6:2055c2667eb3 |
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1 <tool id="rna_star" name="RNA STAR" version="2.5.2b-1" profile="17.01"> | 1 <tool id="rna_star" name="RNA STAR" version="2.5.2b-2" profile="17.01"> |
2 <description>Gapped-read mapper for RNA-seq data</description> | 2 <description>Gapped-read mapper for RNA-seq data</description> |
3 <macros> | 3 <macros> |
4 <import>macros.xml</import> | 4 <import>macros.xml</import> |
5 </macros> | 5 </macros> |
6 <expand macro="requirements"/> | 6 <expand macro="requirements"/> |
118 --chimSegmentMin 12 | 118 --chimSegmentMin 12 |
119 --chimJunctionOverhangMin 12 | 119 --chimJunctionOverhangMin 12 |
120 --alignSJDBoverhangMin 10 | 120 --alignSJDBoverhangMin 10 |
121 --alignMatesGapMax 200000 | 121 --alignMatesGapMax 200000 |
122 --alignIntronMax 200000 | 122 --alignIntronMax 200000 |
123 | 123 --twopassMode Basic |
124 ## --twopass1readsN 100000000 | 124 --twopass1readsN -1 |
125 | |
125 ## --chimSegmentReadGapMax 3 ## not an option in STAR 2.4.0 | 126 ## --chimSegmentReadGapMax 3 ## not an option in STAR 2.4.0 |
126 ## --alignSJstitchMismatchNmax 5 -1 5 5 ## not an option in STAR 2.4.0 | 127 ## --alignSJstitchMismatchNmax 5 -1 5 5 ## not an option in STAR 2.4.0 |
127 | 128 |
128 #elif str( $params.settingsType ) == "full": | 129 #elif str( $params.settingsType ) == "full": |
129 ## Extended parameter options | 130 ## Extended parameter options |
147 --alignSplicedMateMapLminOverLmate "$params.align.alignSplicedMateMapLminOverLmate" | 148 --alignSplicedMateMapLminOverLmate "$params.align.alignSplicedMateMapLminOverLmate" |
148 --alignWindowsPerReadNmax "$params.align.alignWindowsPerReadNmax" | 149 --alignWindowsPerReadNmax "$params.align.alignWindowsPerReadNmax" |
149 --alignTranscriptsPerWindowNmax "$params.align.alignTranscriptsPerWindowNmax" | 150 --alignTranscriptsPerWindowNmax "$params.align.alignTranscriptsPerWindowNmax" |
150 --alignTranscriptsPerReadNmax "$params.align.alignTranscriptsPerReadNmax" | 151 --alignTranscriptsPerReadNmax "$params.align.alignTranscriptsPerReadNmax" |
151 --alignEndsType $params.align.alignEndsType | 152 --alignEndsType $params.align.alignEndsType |
153 | |
154 ## Two pass mode | |
155 --twopassMode "$params.twopass.twopassMode" | |
156 #if str( $params.twopass.twopassMode ) == "Basic": | |
157 --twopass1readsN "$params.twopass.twopass1readsN" | |
158 #end if | |
152 | 159 |
153 ## Chimeric alignment parameter options | 160 ## Chimeric alignment parameter options |
154 #if str( $params.chim.chim_select ) == "yes": | 161 #if str( $params.chim.chim_select ) == "yes": |
155 --chimSegmentMin "$params.chim.chimSegmentMin" | 162 --chimSegmentMin "$params.chim.chimSegmentMin" |
156 --chimScoreMin "$params.chim.chimScoreMin" | 163 --chimScoreMin "$params.chim.chimScoreMin" |
323 <param argument="--alignTranscriptsPerWindowNmax" type="integer" min="1" value="100" label="Maximum number of transcripts per window"/> | 330 <param argument="--alignTranscriptsPerWindowNmax" type="integer" min="1" value="100" label="Maximum number of transcripts per window"/> |
324 <param argument="--alignTranscriptsPerReadNmax" type="integer" min="1" value="10000" label="Maximum number of different alignments per read to consider"/> | 331 <param argument="--alignTranscriptsPerReadNmax" type="integer" min="1" value="10000" label="Maximum number of different alignments per read to consider"/> |
325 <param argument="--alignEndsType" type="boolean" truevalue="EndToEnd" falsevalue="Local" checked="false" label="Use end-to-end read alignments, with no soft-clipping?"/> | 332 <param argument="--alignEndsType" type="boolean" truevalue="EndToEnd" falsevalue="Local" checked="false" label="Use end-to-end read alignments, with no soft-clipping?"/> |
326 </section> | 333 </section> |
327 | 334 |
335 <section name="twopass" title="Two pass mode" expanded="False"> | |
336 <param argument="--twopassMode" type="boolean" truevalue="Basic" falsevalue="None" checked="false" label="Use two pass mode to better map reads to unknown splice junctions"/> | |
337 <param argument="--twopass1readsN" type="integer" min="-1" value="-1" label="Number of reads to map in the first pass (-1: all)"/> | |
338 </section> | |
339 | |
328 <section name="limits" title="Limits" expanded="False"> | 340 <section name="limits" title="Limits" expanded="False"> |
329 <param argument="--limitBAMsortRAM" type="integer" min="0" value="0" label="Maximum available RAM (in bytes) for sorting" help="If 0, this will be set to the genome index size. This is typically only changed in cases where an error is produced." /> | 341 <param argument="--limitBAMsortRAM" type="integer" min="0" value="0" label="Maximum available RAM (in bytes) for sorting" help="If 0, this will be set to the genome index size. This is typically only changed in cases where an error is produced." /> |
330 <param argument="--limitOutSJoneRead" type="integer" min="1" value="1000" label="Maximum number of junctions for one read (including all multimappers)" /> | 342 <param argument="--limitOutSJoneRead" type="integer" min="1" value="1000" label="Maximum number of junctions for one read (including all multimappers)" /> |
331 <param argument="--limitOutSJcollapsed" type="integer" min="1" value="1000000" label="Maximum number of collapsed junctions" /> | 343 <param argument="--limitOutSJcollapsed" type="integer" min="1" value="1000000" label="Maximum number of collapsed junctions" /> |
332 <param argument="--limitSjdbInsertNsj" type="integer" min="0" value="1000000" label="Maximum number of inserts to be inserted into the genome on the fly." /> | 344 <param argument="--limitSjdbInsertNsj" type="integer" min="0" value="1000000" label="Maximum number of inserts to be inserted into the genome on the fly." /> |
333 </section> | 345 </section> |
334 | 346 |
335 <conditional name="chim"> | 347 <conditional name="chim"> |
336 <param name="chim_select" type="select" label="Would you like to set chimeric alignment parameters?"> | 348 <param name="chim_select" type="select" label="Would you like to set chimeric alignment parameters?"> |
337 <option value="no" selected="true">No</option> | 349 <option value="no" selected="true">No</option> |
569 **STAR-Fusion** | 581 **STAR-Fusion** |
570 | 582 |
571 STAR-Fusion_ is used to identify candidate fusion transcripts. The recommended_ parameters for running | 583 STAR-Fusion_ is used to identify candidate fusion transcripts. The recommended_ parameters for running |
572 STAR prior to STAR-Fusion can be pre-selected, with the following exceptions:: | 584 STAR prior to STAR-Fusion can be pre-selected, with the following exceptions:: |
573 | 585 |
574 --twopassMode Basic # not an option in STAR 2.4.0 | |
575 --chimSegmentReadGapMax 3 # not an option in STAR 2.4.0 | 586 --chimSegmentReadGapMax 3 # not an option in STAR 2.4.0 |
576 --alignSJstitchMismatchNmax 5 -1 5 5 # not an option in STAR 2.4.0 | 587 --alignSJstitchMismatchNmax 5 -1 5 5 # not an option in STAR 2.4.0 |
577 | 588 |
578 **Attributions** | 589 **Attributions** |
579 | 590 |