Mercurial > repos > iuc > ribowaltz_process
view ribowaltz_plot.R @ 0:6d4c94373bba draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/ribowaltz commit ff002df702f544829d1b500ac4b517c1e70ad14d
author | iuc |
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date | Thu, 22 Sep 2022 20:30:54 +0000 |
parents | |
children | 042cab870a39 |
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# setup R error handling to go to stderr options(show.error.messages = FALSE, error = function() { cat(geterrmessage(), file = stderr()) q("no", 1, FALSE) }) # we need that to not crash galaxy with an UTF8 error on German LC settings. loc <- Sys.setlocale("LC_MESSAGES", "en_US.UTF-8") library("getopt") options(stringAsFactors = FALSE, useFancyQuotes = FALSE) args <- commandArgs(trailingOnly = TRUE) # get options, using the spec as defined by the enclosed list. # we read the options from the default: commandArgs(TRUE). spec <- matrix(c( "quiet", "q", 0, "logical", "help", "h", 0, "logical", "input_rdata", "i", 1, "character", "params_rlength_distr", "r", 0, "character", "params_rends_heat", "e", 0, "character", "region_psite_plot", "R", 0, "logical", "params_trint_periodicity", "t", 0, "character", "params_metaplots", "m", 0, "character", "params_codon_usage_psite", "u", 0, "character" ), byrow = TRUE, ncol = 4) opt <- getopt(spec) # if help was asked for print a friendly message # and exit with a non-zero error code if (!is.null(opt$help)) { cat(getopt(spec, usage = TRUE)) q(status = 1) } verbose <- is.null(opt$quiet) library("riboWaltz") library("jsonlite") load(opt$input_rdata) if (!is.null(opt$params_rlength_distr)) { pdf("read_lengths.pdf") json_rlength_distr <- fromJSON(opt$params_rlength_distr) length_dist <- rlength_distr( reads_psite_list, sample = names(reads_psite_list), cl = json_rlength_distr$cl, multisamples = json_rlength_distr$multisamples, plot_style = json_rlength_distr$plot_style ) print(length_dist) dev.off() } if (!is.null(opt$params_rends_heat)) { pdf("read_ends_heatmap.pdf", height = 5 * length(reads_psite_list), width = 15) json_rends_heat <- fromJSON(opt$params_rends_heat) for (sample_name in names(reads_psite_list)) { ends_heatmap <- rends_heat( reads_psite_list, annotation_dt, sample = sample_name, cl = json_rends_heat$cl, utr5l = json_rends_heat$utr5l, cdsl = json_rends_heat$cdsl, utr3l = json_rends_heat$utr3l ) print(ends_heatmap[["plot"]]) } dev.off() } if (!is.null(opt$region_psite_plot)) { pdf("psites_per_region.pdf", height = 12, width = 7 * length(reads_psite_list)) psite_region <- region_psite(reads_psite_list, annotation_dt, sample = names(reads_psite_list)) print(psite_region[["plot"]]) dev.off() } if (!is.null(opt$params_trint_periodicity)) { pdf("trinucleotide_periodicity.pdf", height = 6 * length(reads_psite_list), width = 10) json_trint_periodicity <- fromJSON(opt$params_trint_periodicity) frames_stratified <- frame_psite_length( reads_psite_list, sample = names(reads_psite_list), cl = json_trint_periodicity$cl, region = json_trint_periodicity$region, length_range = json_trint_periodicity$length_range ) frames_stratified[["plot"]] frames <- frame_psite_length( reads_psite_list, sample = names(reads_psite_list), region = json_trint_periodicity$region, length_range = json_trint_periodicity$length_range ) print(frames[["plot"]]) dev.off() } if (!is.null(opt$params_metaplots)) { pdf("metaplots.pdf", height = 5 * length(reads_psite_list), width = 24) json_metaplots <- fromJSON(opt$params_metaplots) metaprofile <- metaprofile_psite( reads_psite_list, annotation_dt, sample = names(reads_psite_list), multisamples = json_metaplots$multisamples, plot_style = json_metaplots$plot_style, length_range = json_metaplots$length_range, frequency = json_metaplots$frequency, utr5l = json_metaplots$utr5l, cdsl = json_metaplots$cdsl, utr3l = json_metaplots$utr3l, plot_title = "sample.transcript.length_range" ) print(metaprofile) sample_list <- list() for (sample_name in names(reads_psite_list)) { sample_list[[sample_name]] <- c(sample_name) } metaheatmap <- metaheatmap_psite( reads_psite_list, annotation_dt, sample = sample_list, length_range = json_metaplots$length_range, utr5l = json_metaplots$utr5l, cdsl = json_metaplots$cdsl, utr3l = json_metaplots$utr3l, plot_title = "Comparison metaheatmap" ) print(metaheatmap[["plot"]]) dev.off() } if (!is.null(opt$params_codon_usage_psite)) { pdf("codon_usage.pdf", height = 6, width = 16) json_codon_usage_psite <- fromJSON(opt$params_codon_usage_psite) for (sample_name in names(reads_psite_list)) { cu_barplot <- codon_usage_psite( reads_psite_list, annotation_dt, sample = sample_name, fastapath = json_codon_usage_psite$fastapath, fasta_genome = FALSE, frequency_normalization = json_codon_usage_psite$frequency ) print(cu_barplot[["plot"]]) } dev.off() }