# HG changeset patch # User iuc # Date 1660137299 0 # Node ID 675ee1aa59527908042b9d437bf501a5d3a91148 # Parent 378e55aeeb2529aba935920cd98ca4394c9e8da0 planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spades commit 35f71aa486d8754ee6a8387659032fc7c93d1be3 diff -r 378e55aeeb25 -r 675ee1aa5952 macros.xml --- a/macros.xml Wed Jul 13 07:41:56 2022 +0000 +++ b/macros.xml Wed Aug 10 13:14:59 2022 +0000 @@ -1,6 +1,6 @@ 3.15.4 - 1 + 2 spades @@ -43,6 +43,9 @@ + @@ -738,7 +741,7 @@ SPAdes - St. Petersburg genome assembler - is an assembly toolkit containing various assembly pipelines. `_ (e.g. assemble using k-mer lengths 21,33,55,77,99,127). However, due to increased error rate some changes of k-mer lengths (e.g. selection of shorter ones) may be required. For example, if you ran SPAdes with k-mer lengths 21,33,55,77 and then decided to assemble the same data set using more iterations and larger values of K, you can run SPAdes once again specifying the same output folder and the following options: --restart-from k77 -k 21,33,55,77,99,127 --mismatch-correction -o . Do not forget to copy contigs and scaffolds from the previous run. We're planning to tackle issue of selecting k-mer lengths for IonTorrent reads in next versions. +The selection of k-mer length is non-trivial for IonTorrent. If the dataset is more or less conventional (good coverage, not high GC, etc), then use our `recommendation for long reads `_ (e.g. assemble using k-mer lengths 21,33,55,77,99,127). However, due to increased error rate some changes of k-mer lengths (e.g. selection of shorter ones) may be required. For example, if you ran SPAdes with k-mer lengths 21,33,55,77 and then decided to assemble the same data set using more iterations and larger values of K, you can run SPAdes once again specifying the same output folder and the following options: --restart-from k77 -k 21,33,55,77,99,127 --mismatch-correction -o . Do not forget to copy contigs and scaffolds from the previous run. We're planning to tackle issue of selecting k-mer lengths for IonTorrent reads in next versions. You may need no error correction for Hi-Q enzyme at all. However, we suggest trying to assemble your data with and without error correction and select the best variant. diff -r 378e55aeeb25 -r 675ee1aa5952 rnaspades.xml --- a/rnaspades.xml Wed Jul 13 07:41:56 2022 +0000 +++ b/rnaspades.xml Wed Aug 10 13:14:59 2022 +0000 @@ -18,7 +18,7 @@ @PREPROCESS_CONTIGS_FILES@ @PREPROCESS_FL_RNA_FILES@ - +@OMP_THREADS@ ## run rnaspades.py -o 'output' @@ -238,7 +238,7 @@ **References** -More information can be found on on `github `_ and on the `project website `_. +More information can be found on on `github `_. ]]> 10.1101/420208