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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/cram_to_bam commit cd62639660bef041ba14ecff337fb98e84e75d8a
author | iuc |
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date | Mon, 20 Nov 2023 22:14:11 +0000 |
parents | 55ae400c0cc4 |
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<tool id="samtools_cram_to_bam" name="samtools CRAM to BAM" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@"> <description>convert CRAM alignments to BAM format</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <expand macro="stdio"/> <expand macro="version_command"/> <command><![CDATA[ @ADDTHREADS@ ln -s '$input' input.cram && #if str( $input.metadata.cram_index ) != "None": ln -f -s '${input.metadata.cram_index}' input.cram.crai && #end if @PREPARE_FASTA_IDX@ samtools view #if $parameter_regions.target_region == "regions_bed_file" -L '$parameter_regions.regions_bed_file' #end if -@ \$addthreads -b -T "\$reffa" -o '$output_alignment' input.cram #if $parameter_regions.target_region == "region" '$parameter_regions.region_string' #end if ]]></command> <inputs> <param name="input" type="data" format="cram" label="CRAM alignment file"/> <expand macro="mandatory_reference" argument="-t"/> <conditional name="parameter_regions"> <param name="target_region" type="select" label="Choose conversion within specific genomic region(s)"> <option value="entire_input_file">Entire BAM alignment file</option> <option value="region">Specific region</option> <option value="regions_bed_file">List of specific regions (BED file)</option> </param> <when value="entire_input_file" /> <when value="region"> <param name="region_string" type="text" label="Samtools: region in which pileup is generated" help="e.g. chrX or chr:start-end" /> </when> <when value="regions_bed_file"> <param name="regions_bed_file" argument="-L" type="data" format="bed" label="Only include reads overlapping this BED file" /> </when> </conditional> </inputs> <outputs> <data name="output_alignment" format="bam" label="$tool.name on ${on_string}.bam"></data> </outputs> <tests> <test> <param name="input" value="test.cram" ftype="cram" /> <param name="addref_select" value="history" /> <param name="ref" value="test.fa" /> <output name="output_alignment" file="test.bam" ftype="bam" lines_diff="4" /> </test> <test> <param name="input" value="test.cram" ftype="cram" /> <param name="addref_select" value="history" /> <param name="ref" value="test.fa" /> <param name="target_region" value="region" /> <param name="region_string" value="CHROMOSOME_I" /> <output name="output_alignment" file="test.bam" ftype="bam" lines_diff="4" /> </test> <test> <param name="input" value="test.cram" ftype="cram" /> <param name="addref_select" value="history" /> <param name="ref" value="test.fa" /> <param name="target_region" value="regions_bed_file" /> <param name="regions_bed_file" value="test.bed" ftype="bed" /> <output name="output_alignment" file="test.bam" ftype="bam" lines_diff="4" /> </test> <test> <param name="input" value="test2.cram" dbkey="equCab2" ftype="cram" /> <param name="addref_select" value="cached" /> <param name="ref" value="equCab2chrM" /> <param name="target_region" value="entire_input_file" /> <output name="output_alignment" file="sam_to_bam_out2.bam" ftype="bam" lines_diff="4" /> </test> </tests> <help><![CDATA[ **What this tool does** Converts alignments from the CRAM format to the BAM format using the ``samtools view`` command. ]]></help> <expand macro="citations"/> </tool>