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view samtools_fixmate.xml @ 2:41e36c99a008 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tool_collections/samtools/samtools_fixmate commit 5cb103832529f17e5c72e7f122758c13519fbe5e
author | iuc |
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date | Mon, 15 Aug 2022 09:17:26 +0000 |
parents | 595ae1ba934a |
children | e5ece8fa99de |
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<tool id="samtools_fixmate" name="Samtools fixmate" version="@TOOL_VERSION@+galaxy0" profile="@PROFILE@"> <description>fill mate coordinates, ISIZE and mate related flags</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <expand macro="stdio"/> <expand macro="version_command"/> <command><![CDATA[ @ADDTHREADS@ ## name sort input #if not $bamfile.is_of_type('qname_sorted.bam', 'qnamed_input_sorted.bam'): samtools sort -@ \$addthreads -m \${GALAXY_MEMORY_MB:-768}M -T "\${TMPDIR:-.}" -n -O BAM -o namesorted.bam '$bamfile' && #else: ln -s '$bamfile' namesorted.bam && #end if samtools fixmate -@ \$addthreads $remsec $noprop $tempcigar $addms -O BAM namesorted.bam '$output' ]]></command> <inputs> <param name="bamfile" type="data" format="sam,bam,cram" optional="false" label="Select alignment" help="Set of aligned reads." /> <param name="remsec" type="boolean" argument="-r" truevalue="-r" falsevalue="" checked="false" label="Remove secondary and unmapped reads" /> <param name="noprop" type="boolean" argument="-p" truevalue="-p" falsevalue="" checked="false" label="Disable FR proper pair check" /> <param name="tempcigar" type="boolean" argument="-c" truevalue="-c" falsevalue="" checked="false" label="Add template cigar ct tag" /> <param name="addms" type="boolean" argument="-m" truevalue="-m" falsevalue="" checked="false" label="Add ms (mate score) tags" help="These are used by markdup to select the best reads to keep." /> </inputs> <outputs> <!--<data name="output" format="sam" />--> <data name="output" format="qname_sorted.bam"/> </outputs> <tests> <!-- from https://github.com/samtools/samtools/blob/6d79411685d8f0fbb34e123f52d72b63271f4dcb/test/test.pl#L2493--> <!-- test_cmd($opts,out=>'fixmate/1_coord_sort.sam.expected', err=>'fixmate/1_coord_sort.sam.expected.err', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/1_coord_sort.sam -", expect_fail=>1);--> <test> <param name="bamfile" value="2_isize_overflow.sam" ftype="sam" /> <output name="output" file="2_isize_overflow.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!-- test_cmd($opts,out=>'fixmate/2_isize_overflow.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/2_isize_overflow.sam -");--> <test> <param name="bamfile" value="3_reverse_read_pp_lt.sam" ftype="sam" /> <output name="output" file="3_reverse_read_pp_lt.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!-- test_cmd($opts,out=>'fixmate/3_reverse_read_pp_lt.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/3_reverse_read_pp_lt.sam -");--> <test> <param name="bamfile" value="4_reverse_read_pp_equal.sam" ftype="sam" /> <output name="output" file="4_reverse_read_pp_equal.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!-- test_cmd($opts,out=>'fixmate/4_reverse_read_pp_equal.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/4_reverse_read_pp_equal.sam -");--> <test> <param name="bamfile" value="5_ct.sam" ftype="sam" /> <param name="tempcigar" value="-c" /> <output name="output" file="5_ct.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!-- test_cmd($opts,out=>'fixmate/5_ct.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -cO sam $$opts{path}/fixmate/5_ct.sam -");--> <test> <param name="bamfile" value="6_ct_replace.sam" ftype="sam" /> <param name="tempcigar" value="-c" /> <output name="output" file="6_ct_replace.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!-- test_cmd($opts,out=>'fixmate/6_ct_replace.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -cO sam $$opts{path}/fixmate/6_ct_replace.sam -");--> <test> <param name="bamfile" value="7_two_read_mapped.sam" ftype="sam" /> <output name="output" file="7_two_read_mapped.bam.expected" ftype="qname_sorted.bam" lines_diff="4" /> </test> <!--test_cmd($opts,out=>'fixmate/7_two_read_mapped.bam.expected', cmd=>"$$opts{bin}/samtools fixmate${threads} -O sam $$opts{path}/fixmate/7_two_read_mapped.sam -");--> </tests> <help> **What it does** Fill in mate coordinates, ISIZE and mate related flags from a name-sorted alignment. </help> <expand macro="citations"/> </tool>