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author | iuc |
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date | Mon, 23 Jun 2014 04:58:20 -0400 |
parents | f627c4b93c55 |
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<tool id="samtools_sort" name="sort" version="1.0.2"> <requirements> <requirement type="package" version="0.1.19">samtools</requirement> </requirements> <description>a BAM file</description> <command> samtools sort $sort_mode "${input1}" foo 2>&1 || echo "Error running samtools sort." >&2 ; mv foo.bam "${output1}" </command> <inputs> <param name="input1" type="data" format="bam" label="BAM File" /> <param name="sort_mode" type="select" label="Sort by "> <option value="" selected="True">Chromosomal coordinates</option> <option value="-n">Read names</option> </param> </inputs> <outputs> <data name="output1" format="bam" /> </outputs> <tests> <test> <param name="input1" value="1.bam" ftype="bam" /> <output name="output1" file="1_sort.bam" ftype="bam" sort="True"/> </test> </tests> <help> **What it does** This tool uses the SAMTools_ toolkit to sort alignments by leftmost coordinates or read names. .. _SAMTools: http://samtools.sourceforge.net/samtools.shtml ------ **Citation** For the underlying tool, please cite `Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, Marth G, Abecasis G, Durbin R; 1000 Genome Project Data Processing Subgroup. The Sequence Alignment/Map format and SAMtools. Bioinformatics. 2009 Aug 15;25(16):2078-9. <http://www.ncbi.nlm.nih.gov/pubmed/19505943>`_ If you use this tool in Galaxy, please cite Blankenberg D, et al. *In preparation.* </help> </tool>