Mercurial > repos > iuc > scanpy_inspect

annotate inspect.xml @ 5:08192eebb47d draft

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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 6b5d0d6f038ebd0fae5dbca02ada51555518ed85"
author iuc
date Mon, 10 Feb 2020 05:29:16 -0500
parents cc0deb593fc8
children 5e9171dc8244
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1 <tool id="scanpy_inspect" name="Inspect and manipulate" version="@galaxy_version@" profile="@profile@">
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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2 <description> with scanpy</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 <xml name="score_genes_params">
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6 <param argument="n_bins" type="integer" value="25" label="Number of expression level bins for sampling" help=""/>
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7 <param argument="random_state" type="integer" value="0" label="Random seed for sampling" help=""/>
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8 <expand macro="param_use_raw"/>
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9 </xml>
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10 <token name="@CMD_score_genes_inputs@"><![CDATA[
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11 n_bins=$method.n_bins,
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12 random_state=$method.random_state,
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13 use_raw=$method.use_raw,
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14 copy=False
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15 ]]></token>
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16 <xml name="corr_method">
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17 <param argument="corr_method" type="select" label="P-value correction method">
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18 <option value="benjamini-hochberg">Benjamini-Hochberg</option>
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19 <option value="bonferroni">Bonferroni</option>
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20 </param>
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21 </xml>
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22 <xml name="fit_intercept">
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23 <param argument="fit_intercept" type="boolean" truevalue="True" falsevalue="False" checked="true"
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24 label="Should a constant (a.k.a. bias or intercept) be added to the decision function?" help=""/>
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25 </xml>
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26 <xml name="max_iter">
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27 <param argument="max_iter" type="integer" min="0" value="100" label="Maximum number of iterations taken for the solvers to converge" help=""/>
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28 </xml>
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29 <xml name="multi_class">
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30 <param argument="multi_class" type="select" label="Multi class" help="">
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31 <option value="ovr">ovr: a binary problem is fit for each label</option>
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32 <option value="multinomial">multinomial: the multinomial loss fit across the entire probability distribution, even when the data is binary</option>
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33 <option value="auto">auto: selects ‘ovr’ if the data is binary and otherwise selects ‘multinomial’</option>
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34 </param>
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35 </xml>
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36 <xml name="penalty">
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37 <param argument="penalty" type="select" label="Norm used in the penalization" help="">
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38 <option value="l1">l1</option>
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39 <option value="l2">l2</option>
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40 <option value="customized">customized</option>
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41 </param>
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42 </xml>
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43 <xml name="custom_penalty">
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44 <param argument="pen" type="text" value="" label="Norm used in the penalization" help=""/>
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45 </xml>
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46 <xml name="random_state">
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47 <param argument="random_state" type="integer" value="" optional="true"
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48 label="The seed of the pseudo random number generator to use when shuffling the data" help=""/>
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49 </xml>
0
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50 </macros>
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51 <expand macro="requirements"/>
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52 <expand macro="version_command"/>
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53 <command detect_errors="exit_code"><![CDATA[
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54 @CMD@
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55 ]]></command>
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56 <configfiles>
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57 <configfile name="script_file"><![CDATA[
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58 @CMD_imports@
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59 @CMD_read_inputs@
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60
1
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61 #if $method.method == "pp.calculate_qc_metrics"
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62 sc.pp.calculate_qc_metrics(
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63 adata=adata,
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64 expr_type='$method.expr_type',
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65 var_type='$method.var_type',
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66 #if str($method.qc_vars) != ''
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67 #set $qc_vars = [str(x.strip()) for x in str($method.qc_vars).split(',')]
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68 qc_vars=$qc_vars,
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69 #end if
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70 #if str($method.percent_top) != ''
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71 #set $percent_top = [int(x.strip()) for x in str($method.percent_top).split(',')]
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72 percent_top=$method.percent_top,
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73 #end if
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74 inplace=True)
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75
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76 #else if $method.method == "tl.score_genes"
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77 sc.tl.score_genes(
0
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78 adata=adata,
1
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79 #set $gene_list = [str(x.strip()) for x in str($method.gene_list).split(',')]
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80 gene_list=$gene_list,
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81 ctrl_size=$method.ctrl_size,
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82 score_name='$method.score_name',
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83 #if $method.gene_pool
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iuc
parents: 0
diff changeset
84 #set $gene_pool = [str(x.strip()) for x in $method.gene_pool.split(',')]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
85 gene_pool=$gene_pool,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
86 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
87 @CMD_score_genes_inputs@)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
88
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
89 #else if $method.method == "tl.score_genes_cell_cycle"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
90 #if str($method.s_genes.format) == 'file'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
91 with open('$method.s_genes.file', 'r') as s_genes_f:
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
92 s_genes = [str(x.strip()) for x in s_genes_f.readlines()]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
93 print(s_genes)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
94 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
95
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
96 #if str($method.g2m_genes.format) == 'file'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
97 with open('$method.g2m_genes.file', 'r') as g2m_genes_f:
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
98 g2m_genes = [str(x.strip()) for x in g2m_genes_f.readlines()]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
99 print(g2m_genes)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
100 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
101
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
102 sc.tl.score_genes_cell_cycle(
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
103 adata=adata,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
104 #if str($method.s_genes.format) == 'text'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
105 #set $s_genes = [str(x.strip()) for x in $method.s_genes.text.split(',')]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
106 s_genes=$s_genes,
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iuc
parents: 0
diff changeset
107 #else if str($method.s_genes.format) == 'file'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
108 s_genes=s_genes,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
109 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
110 #if str($method.g2m_genes.format) == 'text'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
111 #set $g2m_genes = [str(x.strip()) for x in $method.g2m_genes.text.split(',')]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
112 g2m_genes=$g2m_genes,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
113 #else if str($method.g2m_genes.format) == 'file'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
114 g2m_genes=g2m_genes,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
115 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
116 @CMD_score_genes_inputs@)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
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117
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iuc
parents: 0
diff changeset
118 #else if $method.method == 'pp.neighbors'
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iuc
parents: 0
diff changeset
119 sc.pp.neighbors(
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iuc
parents: 0
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120 adata=adata,
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iuc
parents: 0
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121 n_neighbors=$method.n_neighbors,
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iuc
parents: 0
diff changeset
122 #if str($method.n_pcs) != ''
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iuc
parents: 0
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123 n_pcs=$method.n_pcs,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
124 #end if
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iuc
parents: 0
diff changeset
125 #if str($method.use_rep) != ''
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iuc
parents: 0
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126 use_rep='$method.use_rep',
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iuc
parents: 0
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127 #end if
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iuc
parents: 0
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128 knn=$method.knn,
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iuc
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129 random_state=$method.random_state,
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iuc
parents: 0
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130 method='$method.pp_neighbors_method',
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iuc
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131 metric='$method.metric',
0
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iuc
parents:
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132 copy=False)
1
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iuc
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133
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iuc
parents: 0
diff changeset
134 #else if $method.method == 'tl.rank_genes_groups'
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iuc
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135 sc.tl.rank_genes_groups(
0
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iuc
parents:
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136 adata=adata,
1
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iuc
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137 groupby='$method.groupby',
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iuc
parents: 0
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138 #if str($method.groups) != ''
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iuc
parents: 0
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139 #set $group=[x.strip() for x in str($method.groups).split(',')]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
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140 groups=$group,
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iuc
parents: 0
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141 #end if
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iuc
parents: 0
diff changeset
142 #if $method.ref.rest == 'rest'
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iuc
parents: 0
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143 reference='$method.ref.rest',
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iuc
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144 #else
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iuc
parents: 0
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145 reference='$method.ref.reference',
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iuc
parents: 0
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146 #end if
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iuc
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147 n_genes=$method.n_genes,
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iuc
parents: 0
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148 method='$method.tl_rank_genes_groups_method.method',
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iuc
parents: 0
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149 #if $method.tl_rank_genes_groups_method.method != 'logreg'
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iuc
parents: 0
diff changeset
150 corr_method='$method.tl_rank_genes_groups_method.corr_method',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
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151 #else
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iuc
parents: 0
diff changeset
152 solver='$method.tl_rank_genes_groups_method.solver.solver',
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iuc
parents: 0
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153 #if $method.tl_rank_genes_groups_method.solver.solver == 'newton-cg'
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iuc
parents: 0
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154 penalty='l2',
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iuc
parents: 0
diff changeset
155 fit_intercept=$method.tl_rank_genes_groups_method.solver.fit_intercept,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
156 max_iter=$method.tl_rank_genes_groups_method.solver.max_iter,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
157 multi_class='$method.tl_rank_genes_groups_method.solver.multi_class',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
158 #else if $method.tl_rank_genes_groups_method.solver.solver == 'lbfgs'
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iuc
parents: 0
diff changeset
159 penalty='l2',
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iuc
parents: 0
diff changeset
160 fit_intercept=$method.tl_rank_genes_groups_method.solver.fit_intercept,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
161 max_iter=$method.tl_rank_genes_groups_method.solver.max_iter,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
162 multi_class='$method.tl_rank_genes_groups_method.solver.multi_class',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
163 #else if $method.tl_rank_genes_groups_method.solver.solver == 'liblinear'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
164 #if $method.tl_rank_genes_groups_method.solver.penalty.penalty == 'l1'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
165 penalty='l1',
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iuc
parents: 0
diff changeset
166 #else if $method.tl_rank_genes_groups_method.solver.penalty.penalty == 'l2'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
167 penalty='l2',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
168 dual=$method.tl_rank_genes_groups_method.solver.penalty.dual,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
169 #else
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
170 penalty='$method.tl_rank_genes_groups_method.solver.penalty.pen',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
171 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
172 fit_intercept=$method.tl_rank_genes_groups_method.solver.intercept_scaling.fit_intercept,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
173 #if $method.tl_rank_genes_groups_method.solver.intercept_scaling.fit_intercept == 'True'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
174 intercept_scaling=$method.tl_rank_genes_groups_method.solver.intercept_scaling.intercept_scaling,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
175 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
176 #if $method.tl_rank_genes_groups_method.solver.random_state
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
177 random_state=$method.tl_rank_genes_groups_method.solver.random_state,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
178 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
179 #else if $method.tl_rank_genes_groups_method.solver.solver == 'sag'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
180 penalty='l2',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
181 fit_intercept=$method.tl_rank_genes_groups_method.solver.fit_intercept,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
182 #if $method.tl_rank_genes_groups_method.solver.random_state
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
183 random_state=$method.tl_rank_genes_groups_method.solver.random_state,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
184 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
185 max_iter=$method.tl_rank_genes_groups_method.solver.max_iter,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
186 multi_class='$method.tl_rank_genes_groups_method.solver.multi_class',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
187 #else if $method.tl_rank_genes_groups_method.solver.solver == 'saga'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
188 #if $method.tl_rank_genes_groups_method.solver.penalty.penalty == 'l1'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
189 penalty='l1',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
190 #else if $method.tl_rank_genes_groups_method.solver.penalty.penalty == 'l2'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
191 penalty='l2',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
192 #else
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
193 penalty='$method.tl_rank_genes_groups_method.solver.penalty.pen',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
194 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
195 fit_intercept=$method.tl_rank_genes_groups_method.solver.fit_intercept,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
196 multi_class='$method.tl_rank_genes_groups_method.solver.multi_class',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
197 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
198 tol=$method.tl_rank_genes_groups_method.tol,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
199 C=$method.tl_rank_genes_groups_method.c,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
200 #end if
3
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
201 use_raw=$method.use_raw)
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
202
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
203 #else if $method.method == "tl.marker_gene_overlap"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
204 reference_markers = {}
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
205 #for $i, $s in enumerate($method.reference_markers)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
206 #set $list=[x.strip() for x in str($s.values).split(',')]
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
207 reference_markers['$s.key'] = $list
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
208 #end for
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
209
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
210 sc.tl.marker_gene_overlap(
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
211 adata,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
212 reference_markers,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
213 #if str($method.key) != ''
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
214 key='$method.key',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
215 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
216 method='$method.overlap.method',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
217 #if $method.overlap.method == 'overlap_count' and str($method.overlap.normalize) != 'None'
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
218 normalize='$method.overlap.normalize',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
219 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
220 #if str($method.top_n_markers) != ''
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
221 top_n_markers=$method.top_n_markers,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
222 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
223 #if str($method.adj_pval_threshold) != ''
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
224 adj_pval_threshold=$method.adj_pval_threshold,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
225 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
226 #if str($method.key_added) != ''
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
227 key_added='$method.key_added',
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
228 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
229 inplace=True)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
230
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
231 #else if $method.method == "pp.log1p"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
232 sc.pp.log1p(
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
233 data=adata,
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
234 copy=False)
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
235
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
236 #else if $method.method == "pp.scale"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
237 sc.pp.scale(
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
238 data=adata,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
239 zero_center=$method.zero_center,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
240 #if $method.max_value
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
241 max_value=$method.max_value,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
242 #end if
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
243 copy=False)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
244
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
245 #else if $method.method == "pp.sqrt"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
246 sc.pp.sqrt(
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
247 data=adata,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
248 copy=False)
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
249 #end if
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
250
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
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parents:
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251 @CMD_anndata_write_outputs@
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252 ]]></configfile>
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253 </configfiles>
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254 <inputs>
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255 <expand macro="inputs_anndata"/>
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256 <conditional name="method">
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257 <param argument="method" type="select" label="Method used for inspecting">
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258 <option value="pp.calculate_qc_metrics">Calculate quality control metrics, using 'pp.calculate_qc_metrics'</option>
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259 <option value="pp.neighbors">Compute a neighborhood graph of observations, using 'pp.neighbors'</option>
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260 <option value="tl.score_genes">Score a set of genes, using 'tl.score_genes'</option>
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261 <option value="tl.score_genes_cell_cycle">Score cell cycle genes, using 'tl.score_genes_cell_cycle'</option>
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262 <option value="tl.rank_genes_groups">Rank genes for characterizing groups, using 'tl.rank_genes_groups'</option>
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263 <!--<option value="tl.marker_gene_overlap">Calculate an overlap score between data-deriven marker genes and provided markers, using 'tl.marker_gene_overlap'</option>-->
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264 <option value="pp.log1p">Logarithmize the data matrix, using 'pp.log1p'</option>
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265 <option value="pp.scale">Scale data to unit variance and zero mean, using 'pp.scale'</option>
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266 <option value="pp.sqrt">Square root the data matrix, using 'pp.sqrt'</option>
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267 </param>
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268 <when value="pp.calculate_qc_metrics">
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269 <param argument="expr_type" type="text" value="counts" label="Name of kind of values in X"/>
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270 <param argument="var_type" type="text" value="genes" label="The kind of thing the variables are"/>
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271 <param argument="qc_vars" type="text" value="" label="Keys for boolean columns of '.var' which identify variables you could want to control for"
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272 help="Keys separated by a comma"/>
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273 <param argument="percent_top" type="text" value="" label="Proportions of top genes to cover"
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274 help=" Values (integers) are considered 1-indexed, '50' finds cumulative proportion to the 50th most expressed genes. Values separated by a comma.
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275 If empty don't calculate"/>
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276 </when>
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277 <when value="pp.neighbors">
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278 <param argument="n_neighbors" type="integer" min="0" value="15" label="The size of local neighborhood (in terms of number of neighboring data points) used for manifold approximation" help="Larger values result in more global views of the manifold, while smaller values result in more local data being preserved. In general values should be in the range 2 to 100. If 'knn' is 'True', number of nearest neighbors to be searched. If 'knn' is 'False', a Gaussian kernel width is set to the distance of the 'n_neighbors' neighbor."/>
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279 <param argument="n_pcs" type="integer" min="0" value="" optional="true" label="Number of PCs to use" help=""/>
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280 <param argument="use_rep" type="text" value="" optional="true" label="Indicated representation to use" help="If not set, the representation is chosen automatically: for n_vars below 50, X is used, otherwise X_pca (uns) is used. If X_pca is not present, it's computed with default parameter"/>
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281 <param argument="knn" type="boolean" truevalue="True" falsevalue="False" checked="true" label="Use a hard threshold to restrict the number of neighbors to n_neighbors?" help="If true, it considers a knn graph. Otherwise, it uses a Gaussian Kernel to assign low weights to neighbors more distant than the 'n_neighbors' nearest neighbor."/>
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282 <param argument="random_state" type="integer" value="0" label="Numpy random seed" help=""/>
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283 <param name="pp_neighbors_method" argument="method" type="select" label="Method for computing connectivities" help="">
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284 <option value="umap">umap (McInnes et al, 2018)</option>
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285 <option value="gauss">gauss: Gauss kernel following (Coifman et al 2005) with adaptive width (Haghverdi et al 2016)</option>
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286 </param>
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287 <param argument="metric" type="select" label="Distance metric" help="">
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288 <expand macro="distance_metric_options"/>
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289 </param>
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290 </when>
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291 <when value="tl.score_genes">
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292 <param argument="gene_list" type="text" value="" label="The list of gene names used for score calculation" help="Genes separated by a comma"/>
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293 <param argument="ctrl_size" type="integer" value="50" label="Number of reference genes to be sampled"
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294 help="If 'len(gene_list)' is not too low, you can set 'ctrl_size=len(gene_list)'."/>
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295 <param argument="gene_pool" type="text" value="" optional="true" label="Genes for sampling the reference set"
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296 help="Default is all genes. Genes separated by a comma"/>
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297 <expand macro="score_genes_params"/>
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298 <param argument="score_name" type="text" value="score" label="Name of the field to be added in '.obs'" help=""/>
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299 </when>
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300 <when value="tl.score_genes_cell_cycle">
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301 <conditional name='s_genes'>
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302 <param name="format" type="select" label="Format for the list of genes associated with S phase">
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303 <option value="file">File</option>
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304 <option value="text" selected="true">Text</option>
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305 </param>
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306 <when value="text">
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307 <param name="text" type="text" value="" label="List of genes associated with S phase" help="Genes separated by a comma"/>
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308 </when>
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309 <when value="file">
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310 <param name="file" type="data" format="txt" label="File with the list of genes associated with S phase" help="One gene per line"/>
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311 </when>
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312 </conditional>
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313 <conditional name='g2m_genes'>
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314 <param name="format" type="select" label="Format for the list of genes associated with G2M phase">
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315 <option value="file">File</option>
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316 <option value="text" selected="true">Text</option>
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317 </param>
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318 <when value="text">
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319 <param name="text" type="text" value="" label="List of genes associated with G2M phase" help="Genes separated by a comma"/>
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320 </when>
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321 <when value="file">
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322 <param name="file" type="data" format="txt" label="File with the list of genes associated with G2M phase" help="One gene per line"/>
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323 </when>
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324 </conditional>
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325 <expand macro="score_genes_params"/>
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326 </when>
1
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327 <when value="tl.rank_genes_groups">
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328 <param argument="groupby" type="text" value="" label="The key of the observations grouping to consider" help=""/>
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329 <expand macro="param_use_raw"/>
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330 <param argument="groups" type="text" value="" label="Subset of groups to which comparison shall be restricted" help="e.g. ['g1', 'g2', 'g3']. If not passed, a ranking will be generated for all groups."/>
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331 <conditional name="ref">
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332 <param name="rest" type="select" label="Comparison">
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333 <option value="rest">Compare each group to the union of the rest of the group</option>
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334 <option value="group_id">Compare with respect to a specific group</option>
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parents: 0
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335 </param>
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336 <when value="rest"/>
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parents: 0
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337 <when value="group_id">
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338 <param argument="reference" type="text" value="" label="Group identifier with respect to which compare"/>
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339 </when>
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340 </conditional>
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341 <param argument="n_genes" type="integer" min="0" value="100" label="The number of genes that appear in the returned tables" help=""/>
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342 <conditional name="tl_rank_genes_groups_method">
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parents: 0
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343 <param argument="method" type="select" label="Method">
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344 <option value="t-test">t-test</option>
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parents: 0
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345 <option value="wilcoxon">Wilcoxon-Rank-Sum</option>
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346 <option value="t-test_overestim_var" selected="true">t-test with overestimate of variance of each group</option>
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347 <option value="logreg">Logistic regression</option>
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348 </param>
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349 <when value="t-test">
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parents: 0
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350 <expand macro="corr_method"/>
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parents: 0
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351 </when>
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parents: 0
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352 <when value="wilcoxon">
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parents: 0
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353 <expand macro="corr_method"/>
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parents: 0
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354 </when>
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parents: 0
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355 <when value="t-test_overestim_var">
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parents: 0
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356 <expand macro="corr_method"/>
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parents: 0
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357 </when>
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parents: 0
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358 <when value="logreg">
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parents: 0
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359 <conditional name="solver">
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360 <param argument="solver" type="select" label="Algorithm to use in the optimization problem" help="For small datasets, ‘liblinear’ is a good choice, whereas ‘sag’ and ‘saga’ are faster for large ones. For multiclass problems, only ‘newton-cg’, ‘sag’, ‘saga’ and ‘lbfgs’ handle multinomial loss; ‘liblinear’ is limited to one-versus-rest schemes. ‘newton-cg’, ‘lbfgs’ and ‘sag’ only handle L2 penalty, whereas ‘liblinear’ and ‘saga’ handle L1 penalty.">
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parents: 0
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361 <option value="newton-cg">newton-cg</option>
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parents: 0
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362 <option value="lbfgs">lbfgs</option>
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363 <option value="liblinear">liblinear</option>
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364 <option value="sag">sag</option>
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365 <option value="saga">saga</option>
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366 </param>
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parents: 0
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367 <when value="newton-cg">
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parents: 0
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368 <expand macro="fit_intercept"/>
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parents: 0
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369 <expand macro="max_iter"/>
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parents: 0
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370 <expand macro="multi_class"/>
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parents: 0
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371 </when>
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parents: 0
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372 <when value="lbfgs">
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iuc
parents: 0
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373 <expand macro="fit_intercept"/>
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parents: 0
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374 <expand macro="max_iter"/>
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parents: 0
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375 <expand macro="multi_class"/>
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parents: 0
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376 </when>
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parents: 0
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377 <when value="liblinear">
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parents: 0
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378 <conditional name="penalty">
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parents: 0
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379 <expand macro="penalty"/>
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parents: 0
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380 <when value="l1"/>
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parents: 0
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381 <when value="l2">
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382 <param argument="dual" type="boolean" truevalue="True" falsevalue="False" checked="false"
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383 label="Dual (not primal) formulation?" help="Prefer primal when n_samples > n_features"/>
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384 </when>
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parents: 0
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385 <when value="customized">
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parents: 0
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386 <expand macro="custom_penalty"/>
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parents: 0
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387 </when>
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parents: 0
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388 </conditional>
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389 <conditional name="intercept_scaling">
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parents: 0
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390 <param argument="fit_intercept" type="select"
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391 label="Should a constant (a.k.a. bias or intercept) be added to the decision function?" help="">
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392 <option value="True">Yes</option>
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393 <option value="False">No</option>
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394 </param>
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parents: 0
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395 <when value="True">
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parents: 0
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396 <param argument="intercept_scaling" type="float" value="1.0"
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397 label="Intercept scaling"
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398 help="x becomes [x, self.intercept_scaling], i.e. a 'synthetic' feature with constant value equal to intercept_scaling is appended to the instance vector. The intercept becomes intercept_scaling * synthetic_feature_weight."/>
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parents: 0
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399 </when>
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parents: 0
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400 <when value="False"/>
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parents: 0
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401 </conditional>
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parents: 0
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402 <expand macro="random_state"/>
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parents: 0
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403 </when>
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parents: 0
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404 <when value="sag">
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parents: 0
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405 <expand macro="fit_intercept"/>
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iuc
parents: 0
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406 <expand macro="random_state"/>
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iuc
parents: 0
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407 <expand macro="max_iter"/>
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iuc
parents: 0
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408 <expand macro="multi_class"/>
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parents: 0
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409 </when>
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parents: 0
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410 <when value="saga">
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iuc
parents: 0
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411 <conditional name="penalty">
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iuc
parents: 0
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412 <expand macro="penalty"/>
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iuc
parents: 0
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413 <when value="l1"/>
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iuc
parents: 0
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414 <when value="l2"/>
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parents: 0
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415 <when value="customized">
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parents: 0
diff changeset
416 <expand macro="custom_penalty"/>
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parents: 0
diff changeset
417 </when>
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parents: 0
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418 </conditional>
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parents: 0
diff changeset
419 <expand macro="fit_intercept"/>
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parents: 0
diff changeset
420 <expand macro="multi_class"/>
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parents: 0
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421 </when>
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parents: 0
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422 </conditional>
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parents: 0
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423 <param argument="tol" type="float" value="1e-4" label="Tolerance for stopping criteria" help=""/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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424 <param argument="c" type="float" value="1.0" label="Inverse of regularization strength"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
diff changeset
425 help="It must be a positive float. Like in support vector machines, smaller values specify stronger regularization."/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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426 </when>
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parents: 0
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427 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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428 </when>
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parents: 0
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429 <!--<when value="tl.marker_gene_overlap">
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parents: 0
diff changeset
430 <repeat name="reference_markers" title="Marker genes">
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parents: 0
diff changeset
431 <param name="key" type="text" value="" label="Cell identity name" help=""/>
3
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
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parents: 2
diff changeset
432 <param name="values" type="text" value="" label="List of genes" help="Comma-separated names from 'var'"/>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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433 </repeat>
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parents: 0
diff changeset
434 <param argument="key" type="text" value="rank_genes_groups" label="Key in adata.uns where the rank_genes_groups output is stored"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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435 <conditional name="overlap">
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parents: 0
diff changeset
436 <param argument="method" type="select" label="Method to calculate marker gene overlap">
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parents: 0
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437 <option value="overlap_count">overlap_count: Intersection of the gene set</option>
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parents: 0
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438 <option value="overlap_coef">overlap_coef: Overlap coefficient</option>
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parents: 0
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439 <option value="jaccard">jaccard: Jaccard index</option>
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parents: 0
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440 </param>
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parents: 0
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441 <when value="overlap_count">
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parents: 0
diff changeset
442 <param argument="normalize" type="select" label="Normalization option for the marker gene overlap output">
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parents: 0
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443 <option value="None">None</option>
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parents: 0
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444 <option value="reference">reference: Normalization of the data by the total number of marker genes given in the reference annotation per group</option>
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parents: 0
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445 <option value="data">data: Normalization of the data by the total number of marker genes used for each cluster</option>
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parents: 0
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446 </param>
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parents: 0
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447 </when>
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parents: 0
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448 <when value="overlap_coef"/>
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parents: 0
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449 <when value="jaccard"/>
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parents: 0
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450 </conditional>
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parents: 0
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451 <param argument="top_n_markers" type="integer" optional="true" label="Number of top data-derived marker genes to use" help="By default all calculated marker genes are used. If adj_pval_threshold is set along with top_n_markers, then adj_pval_threshold is ignored."/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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452 <param argument="adj_pval_threshold" type="float" optional="true" label="Significance threshold on the adjusted p-values to select marker genes" help=" This can only be used when adjusted p-values are calculated by 'tl.rank_genes_groups'. If adj_pval_threshold is set along with top_n_markers, then adj_pval_threshold is ignored."/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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453 <param argument="key_added" type="text" value="" optional="true" label="Key that will contain the marker overlap scores in 'uns'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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454 </when>-->
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parents: 0
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455 <when value="pp.log1p"/>
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parents: 0
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456 <when value="pp.scale">
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parents: 0
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457 <param argument="zero_center" type="boolean" truevalue="True" falsevalue="False" checked="true"
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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458 label="Zero center?" help="If not, it omits zero-centering variables, which allows to handle sparse input efficiently."/>
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parents: 0
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459 <param argument="max_value" type="float" value="" optional="true" label="Maximum value"
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parents: 0
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460 help="Clip (truncate) to this value after scaling. If not set, it does not clip."/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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461 </when>
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parents: 0
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462 <when value="pp.sqrt"/>
0
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463 </conditional>
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464 </inputs>
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465 <outputs>
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466 <expand macro="anndata_outputs"/>
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467 </outputs>
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468 <tests>
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469 <test>
1
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parents: 0
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470 <!-- test 1 -->
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parents: 0
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471 <param name="adata" value="sparce_csr_matrix.h5ad" />
0
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472 <conditional name="method">
1
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parents: 0
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473 <param name="method" value="pp.calculate_qc_metrics"/>
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parents: 0
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474 <param name="expr_type" value="counts"/>
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parents: 0
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475 <param name="var_type" value="genes"/>
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parents: 0
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476 <param name="qc_vars" value="mito,negative"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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477 <param name="percent_top" value=""/>
0
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478 </conditional>
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479 <assert_stdout>
1
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parents: 0
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480 <has_text_matching expression="sc.pp.calculate_qc_metrics" />
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parents: 0
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481 <has_text_matching expression="expr_type='counts'" />
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parents: 0
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482 <has_text_matching expression="var_type='genes'" />
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parents: 0
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483 <has_text_matching expression="qc_vars=\['mito', 'negative'\]" />
0
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484 </assert_stdout>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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parents: 0
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485 <output name="anndata_out" file="pp.calculate_qc_metrics.sparce_csr_matrix.h5ad" ftype="h5ad" compare="sim_size"/>
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parents: 0
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486 </test>
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parents: 0
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487 <test>
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parents: 0
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488 <!-- test 2 -->
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parents: 0
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489 <param name="adata" value="pp.recipe_weinreb17.paul15_subsample.h5ad" />
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parents: 0
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490 <conditional name="method">
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parents: 0
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491 <param name="method" value="pp.neighbors"/>
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parents: 0
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492 <param name="n_neighbors" value="15"/>
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parents: 0
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493 <param name="knn" value="True"/>
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parents: 0
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494 <param name="random_state" value="0"/>
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parents: 0
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495 <param name="pp_neighbors_method" value="umap"/>
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parents: 0
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496 <param name="metric" value="euclidean"/>
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diff changeset
497 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
498 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
499 <has_text_matching expression="sc.pp.neighbors"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
500 <has_text_matching expression="n_neighbors=15"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
501 <has_text_matching expression="knn=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
502 <has_text_matching expression="random_state=0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
503 <has_text_matching expression="method='umap'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
504 <has_text_matching expression="metric='euclidean'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
505 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
506 <output name="anndata_out" file="pp.neighbors_umap_euclidean.recipe_weinreb17.paul15_subsample.h5ad" ftype="h5ad" compare="sim_size">
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
507 <assert_contents>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
508 <has_h5_keys keys="X, obs, obsm, uns, var" />
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
509 </assert_contents>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
510 </output>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
511 </test>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
512 <test>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
513 <!-- test 3 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
514 <param name="adata" value="pp.recipe_weinreb17.paul15_subsample.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
515 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
516 <param name="method" value="pp.neighbors"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
517 <param name="n_neighbors" value="15"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
518 <param name="knn" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
519 <param name="pp_neighbors_method" value="gauss"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
520 <param name="metric" value="braycurtis"/>
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
521 </conditional>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
522 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
523 <has_text_matching expression="sc.pp.neighbors"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
524 <has_text_matching expression="n_neighbors=15"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
525 <has_text_matching expression="knn=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
526 <has_text_matching expression="random_state=0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
527 <has_text_matching expression="method='gauss'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
528 <has_text_matching expression="metric='braycurtis'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
529 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
530 <output name="anndata_out" file="pp.neighbors_gauss_braycurtis.recipe_weinreb17.paul15_subsample.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
531 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
532 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
533 <!-- test 4 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
534 <param name="adata" value="krumsiek11.h5ad" />
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
535 <conditional name="method">
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
536 <param name="method" value="tl.score_genes"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
537 <param name="gene_list" value="Gata2, Fog1"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
538 <param name="ctrl_size" value="2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
539 <param name="n_bins" value="2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
540 <param name="random_state" value="2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
541 <param name="use_raw" value="False"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
542 <param name="score_name" value="score"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
543 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
544 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
545 <has_text_matching expression="sc.tl.score_genes" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
546 <has_text_matching expression="gene_list=\['Gata2', 'Fog1'\]" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
547 <has_text_matching expression="ctrl_size=2" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
548 <has_text_matching expression="score_name='score'" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
549 <has_text_matching expression="n_bins=2" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
550 <has_text_matching expression="random_state=2" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
551 <has_text_matching expression="use_raw=False" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
552 <has_text_matching expression="copy=False" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
553 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
554 <output name="anndata_out" file="tl.score_genes.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
555 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
556 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
557 <!-- test 5 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
558 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
559 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
560 <param name="method" value="tl.score_genes_cell_cycle"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
561 <conditional name='s_genes'>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
562 <param name="format" value="text"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
563 <param name="text" value="Gata2, Fog1, EgrNab"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
564 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
565 <conditional name='g2m_genes'>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
566 <param name="format" value="text"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
567 <param name="text" value="Gata2, Fog1, EgrNab"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
568 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
569 <param name="n_bins" value="2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
570 <param name="random_state" value="1"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
571 <param name="use_raw" value="False"/>
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
572 </conditional>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
573 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
574 <has_text_matching expression="sc.tl.score_genes_cell_cycle"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
575 <has_text_matching expression="s_genes=\['Gata2', 'Fog1', 'EgrNab'\]"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
576 <has_text_matching expression="g2m_genes=\['Gata2', 'Fog1', 'EgrNab'\]"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
577 <has_text_matching expression="n_bins=2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
578 <has_text_matching expression="random_state=1"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
579 <has_text_matching expression="use_raw=False"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
580 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
581 <output name="anndata_out" file="tl.score_genes_cell_cycle.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
582 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
583 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
584 <!-- test 6 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
585 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
586 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
587 <param name="method" value="tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
588 <param name="groupby" value="cell_type"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
589 <param name="use_raw" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
590 <conditional name="ref">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
591 <param name="rest" value="rest"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
592 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
593 <param name="n_genes" value="100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
594 <conditional name="tl_rank_genes_groups_method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
595 <param name="method" value="t-test_overestim_var"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
596 <param name="corr_method" value="benjamini-hochberg"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
597 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
598 </conditional>
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
599 <assert_stdout>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
600 <has_text_matching expression="sc.tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
601 <has_text_matching expression="groupby='cell_type'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
602 <has_text_matching expression="use_raw=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
603 <has_text_matching expression="reference='rest'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
604 <has_text_matching expression="n_genes=100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
605 <has_text_matching expression="method='t-test_overestim_var'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
606 <has_text_matching expression="corr_method='benjamini-hochberg'"/>
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
607 </assert_stdout>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
608 <output name="anndata_out" file="tl.rank_genes_groups.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
609 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
610 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
611 <!-- test 7 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
612 <param name="adata" value="pbmc68k_reduced.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
613 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
614 <param name="method" value="tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
615 <param name="groupby" value="louvain"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
616 <param name="use_raw" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
617 <conditional name="ref">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
618 <param name="rest" value="rest"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
619 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
620 <param name="n_genes" value="100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
621 <conditional name="tl_rank_genes_groups_method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
622 <param name="method" value="logreg"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
623 <conditional name="solver">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
624 <param name="solver" value="newton-cg"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
625 <param name="fit_intercept" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
626 <param name="max_iter" value="100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
627 <param name="multi_class" value="auto"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
628 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
629 <param name="tol" value="1e-4"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
630 <param name="c" value="1.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
631 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
632 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
633 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
634 <has_text_matching expression="sc.tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
635 <has_text_matching expression="groupby='louvain'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
636 <has_text_matching expression="use_raw=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
637 <has_text_matching expression="reference='rest'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
638 <has_text_matching expression="n_genes=100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
639 <has_text_matching expression="method='logreg'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
640 <has_text_matching expression="solver='newton-cg'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
641 <has_text_matching expression="penalty='l2'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
642 <has_text_matching expression="fit_intercept=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
643 <has_text_matching expression="max_iter=100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
644 <has_text_matching expression="multi_class='auto'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
645 <has_text_matching expression="tol=0.0001"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
646 <has_text_matching expression="C=1.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
647 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
648 <output name="anndata_out" file="tl.rank_genes_groups.newton-cg.pbmc68k_reduced.h5ad" ftype="h5ad" compare="sim_size">
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
649 <assert_contents>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
650 <has_h5_keys keys="X, obs, obsm, raw.X, raw.var, uns, var" />
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
651 </assert_contents>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
652 </output>
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
653 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
654 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
655 <!-- test 8 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
656 <param name="adata" value="pbmc68k_reduced.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
657 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
658 <param name="method" value="tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
659 <param name="groupby" value="louvain"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
660 <param name="use_raw" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
661 <conditional name="ref">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
662 <param name="rest" value="rest"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
663 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
664 <param name="n_genes" value="100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
665 <conditional name="tl_rank_genes_groups_method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
666 <param name="method" value="logreg"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
667 <conditional name="solver">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
668 <param name="solver" value="liblinear"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
669 <conditional name="penalty">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
670 <param name="penalty" value="l2"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
671 <param name="dual" value="False"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
672 <conditional name="intercept_scaling">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
673 <param name="fit_intercept" value="True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
674 <param name="intercept_scaling" value="1.0" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
675 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
676 <param name="random_state" value="1"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
677 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
678 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
679 <param name="tol" value="1e-4"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
680 <param name="c" value="1.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
681 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
682 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
683 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
684 <has_text_matching expression="sc.tl.rank_genes_groups"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
685 <has_text_matching expression="groupby='louvain'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
686 <has_text_matching expression="use_raw=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
687 <has_text_matching expression="reference='rest'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
688 <has_text_matching expression="n_genes=100"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
689 <has_text_matching expression="method='logreg'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
690 <has_text_matching expression="solver='liblinear'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
691 <has_text_matching expression="penalty='l2'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
692 <has_text_matching expression="dual=False"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
693 <has_text_matching expression="fit_intercept=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
694 <has_text_matching expression="intercept_scaling=1.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
695 <has_text_matching expression="tol=0.0001"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
696 <has_text_matching expression="C=1.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
697 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
698 <output name="anndata_out" file="tl.rank_genes_groups.liblinear.krumsiek11.h5ad" ftype="h5ad" compare="sim_size">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
699 <assert_contents>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
700 <has_h5_keys keys="X, obs, obsm, raw.X, raw.var, uns, var" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
701 </assert_contents>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
702 </output>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
703 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
704 <!--<test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
705 < test 9 >
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
706 <param name="adata" value="tl.rank_genes_groups.louvain.neighbors.pca.pbmc68k_reduced.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
707 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
708 <param name="method" value="tl.marker_gene_overlap"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
709 <repeat name="reference_markers">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
710 <param name="key" value="CD4 T cells"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
711 <param name="value" value="IL7R"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
712 </repeat>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
713 <repeat name="reference_markers">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
714 <param name="key" value="CD14+ Monocytes"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
715 <param name="value" value="CD14,LYZ"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
716 </repeat>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
717 <repeat name="reference_markers">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
718 <param name="key" value="B cells"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
719 <param name="value" value="MS4A1"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
720 </repeat>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
721 <conditional name="overlap">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
722 <param argument="method" value="overlap_count"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
723 <param argument="normalize" value="None"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
724 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
725 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
726 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
727 <has_text_matching expression="tl.marker_gene_overlap"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
728 <has_text_matching expression="key='rank_genes_groups'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
729 <has_text_matching expression="method='overlap_count'"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
730 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
731 <output name="anndata_out" file="pp.log1p.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
732 </test>-->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
733 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
734 <!-- test 9 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
735 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
736 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
737 <param name="method" value="pp.log1p"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
738 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
739 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
740 <has_text_matching expression="sc.pp.log1p"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
741 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
742 <output name="anndata_out" file="pp.log1p.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
743 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
744 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
745 <!-- test 10 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
746 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
747 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
748 <param name="method" value="pp.scale"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
749 <param name="zero_center" value="true"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
750 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
751 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
752 <has_text_matching expression="sc.pp.scale"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
753 <has_text_matching expression="zero_center=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
754 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
755 <output name="anndata_out" file="pp.scale.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
756 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
757 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
758 <!-- test 11 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
759 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
760 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
761 <param name="method" value="pp.scale"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
762 <param name="zero_center" value="true"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
763 <param name="max_value" value="10"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
764 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
765 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
766 <has_text_matching expression="sc.pp.scale"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
767 <has_text_matching expression="zero_center=True"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
768 <has_text_matching expression="max_value=10.0"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
769 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
770 <output name="anndata_out" file="pp.scale_max_value.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
771 </test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
772 <test>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
773 <!-- test 12 -->
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
774 <param name="adata" value="krumsiek11.h5ad" />
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
775 <conditional name="method">
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
776 <param name="method" value="pp.sqrt"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
777 </conditional>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
778 <assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
779 <has_text_matching expression="sc.pp.sqrt"/>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
780 </assert_stdout>
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
781 <output name="anndata_out" file="pp.sqrt.krumsiek11.h5ad" ftype="h5ad" compare="sim_size"/>
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
782 </test>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
783 </tests>
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
784 <help><![CDATA[
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
785 Calculate quality control metrics., using `pp.calculate_qc_metrics`
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
786 ===================================================================
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
787
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
788 Calculates a number of qc metrics for an AnnData object, largely based on calculateQCMetrics from scater.
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
789 Currently is most efficient on a sparse CSR or dense matrix.
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
790
3
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
791 It updates the observation level metrics with
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
792
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
793 - total_{var_type}_by_{expr_type} (e.g. "total_genes_by_counts", number of genes with positive counts in a cell)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
794 - total_{expr_type} (e.g. "total_counts", total number of counts for a cell)
3
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
795 - pct_{expr_type}_in_top_{n}_{var_type} - for n in percent_top (e.g. "pct_counts_in_top_50_genes", cumulative percentage of counts for 50 most expressed genes in a cell)
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
796 - total_{expr_type}_{qc_var} - for qc_var in qc_vars (e.g. "total_counts_mito", total number of counts for variabes in qc_vars)
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
797 - pct_{expr_type}_{qc_var} - for qc_var in qc_vars (e.g. "pct_counts_mito", proportion of total counts for a cell which are mitochondrial)
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
798
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
799 And also the variable level metrics:
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
800
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
801 - total_{expr_type} (e.g. "total_counts", sum of counts for a gene)
3
cc0deb593fc8 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 3b41d687ff30583540d055f6995de00530cca81d"
iuc
parents: 2
diff changeset
802 - mean_{expr_type} (e.g. "mean counts", mean expression over all cells)
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
803 - n_cells_by_{expr_type} (e.g. "n_cells_by_counts", number of cells this expression is measured in)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
804 - pct_dropout_by_{expr_type} (e.g. "pct_dropout_by_counts", percentage of cells this feature does not appear in)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
805
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
806 More details on the `scanpy documentation
5
08192eebb47d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 6b5d0d6f038ebd0fae5dbca02ada51555518ed85"
iuc
parents: 3
diff changeset
807 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.pp.calculate_qc_metrics.html>`__
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
808
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
809 Compute a neighborhood graph of observations, using `pp.neighbors`
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
810 ==================================================================
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
811
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
812 The neighbor search efficiency of this heavily relies on UMAP (McInnes et al, 2018),
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
813 which also provides a method for estimating connectivities of data points -
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
814 the connectivity of the manifold (`method=='umap'`). If `method=='diffmap'`,
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
815 connectivities are computed according to Coifman et al (2005), in the adaption of
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
816 Haghverdi et al (2016).
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
817
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
818 The returned AnnData object contains:
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
819
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
820 - Weighted adjacency matrix of the neighborhood graph of data points (connectivities). Weights should be interpreted as connectivities.
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
821 - Distances for each pair of neighbors (distances)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
822
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
823 This data are stored in the unstructured annotation (uns) and can be accessed using the inspect tool for AnnData objects
0
5d2e17328afe planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 92f85afaed0097d1879317a9f513093fce5481d6
iuc
parents:
diff changeset
824
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
825 More details on the `scanpy documentation
5
08192eebb47d "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 6b5d0d6f038ebd0fae5dbca02ada51555518ed85"
iuc
parents: 3
diff changeset
826 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.pp.neighbors.html>`__
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
827
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
828 Score a set of genes, using `tl.score_genes`
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
829 ============================================
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
830
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
831 The score is the average expression of a set of genes subtracted with the
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
iuc
parents: 0
diff changeset
832 average expression of a reference set of genes. The reference set is
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833 randomly sampled from the `gene_pool` for each binned expression value.
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834
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835 This reproduces the approach in Seurat (Satija et al, 2015) and has been implemented
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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836 for Scanpy by Davide Cittaro.
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837
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838 More details on the `scanpy documentation
5
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839 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.tl.score_genes.html>`__
1
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840
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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841 Score cell cycle genes, using `tl.score_genes_cell_cycle`
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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842 =========================================================
0
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843
1
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844 Given two lists of genes associated to S phase and G2M phase, calculates
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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845 scores and assigns a cell cycle phase (G1, S or G2M). See
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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diff changeset
846 `score_genes` for more explanation.
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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847
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848 More details on the `scanpy documentation
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849 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.tl.score_genes_cell_cycle.html>`__
1
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850
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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851 Rank genes for characterizing groups, using `tl.rank_genes_groups`
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852 ==================================================================
0
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853
1
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854 The returned AnnData object contains:
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855
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856 - Gene names, ordered according to scores
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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857 - Z-score underlying the computation of a p-value for each gene for each group, prdered according to scores
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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858 - Log2 fold change for each gene for each group, ordered according to scores. It is only provided if method is ‘t-test’ like. This is an approximation calculated from mean-log values.
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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859 - P-values
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860 - Ajusted p-values
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861
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862 This data are stored in the unstructured annotation (uns) and can be accessed using the inspect tool for AnnData objects
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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863
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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864 More details on the `scanpy documentation
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865 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.tl.rank_genes_groups.html>`__
0
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866
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867
1
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868 Calculate an overlap score between data-deriven marker genes and provided markers (`tl.marker_gene_overlap`)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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869 ============================================================================================================
0
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870
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871 Marker gene overlap scores can be quoted as overlap counts, overlap coefficients, or jaccard indices. The method returns a pandas dataframe which can be used to annotate clusters based on marker gene overlaps.
0
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872
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873
1
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874 Logarithmize the data matrix (`pp.log1p`)
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875 =========================================
0
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876
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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877 More details on the `scanpy documentation
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878 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.pp.log1p.html>`__
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879
1
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880 Scale data to unit variance and zero mean (`pp.scale`)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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881 ======================================================
0
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882
1
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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883 More details on the `scanpy documentation
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884 <https://icb-scanpy.readthedocs-hosted.com/en/@version@/api/scanpy.pp.scale.html>`__
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885
1
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886 Computes the square root the data matrix (`pp.sqrt`)
a755eaa1cc32 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scanpy/ commit 8ef5f7c6f8728608a3f05bb51e11b642b84a05f5"
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887 ====================================================
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888
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889 `X = sqrt(X)`
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890 ]]></help>
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891 <expand macro="citations"/>
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892 </tool>