--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/scater-plot-tsne.R	Tue Sep 03 14:25:32 2019 -0400
@@ -0,0 +1,69 @@
+#!/usr/bin/env Rscript
+
+# Creates a t-SNE plot of a normalised SingleCellExperiment object.
+
+# Load optparse we need to check inputs
+
+library(optparse)
+library(workflowscriptscommon)
+library(LoomExperiment)
+library(scater)
+library(Rtsne)
+
+# parse options
+
+option_list = list(
+  make_option(
+    c("-i", "--input-loom"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "A SingleCellExperiment object file in Loom format."
+  ),
+  make_option(
+    c("-c", "--colour-by"),
+    action = "store",
+    default = NULL,
+    type = 'character',
+    help = "Feature (from annotation file) to colour t-SNE plot points by. The values represented in this options should be categorical"
+  ),
+  make_option(
+    c("-s", "--size-by"),
+    action = "store",
+    default = NULL,
+    type = 'character',
+    help = "Feature (from annotation file) to size t-SNE plot points by. The values represented in this options should be numerical and not categorical"
+  ),
+  make_option(
+    c("-p", "--shape-by"),
+    action = "store",
+    default = NULL,
+    type = 'character',
+    help = "Feature (from annotation file) to shape t-SNE plot points by. The values represented in this options should be categorical"
+  ),
+  make_option(
+    c("-o", "--output-plot-file"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "Path of the PDF output file to save plot to."
+  )
+)
+
+opt <- wsc_parse_args(option_list, mandatory = c('input_loom', 'output_plot_file'))
+# Check parameter values
+
+if ( ! file.exists(opt$input_loom)){
+  stop((paste('File', opt$input_loom, 'does not exist')))
+}
+
+
+# Input from Loom format
+
+scle <- import(opt$input_loom, format='loom', type='SingleCellLoomExperiment')
+scle <- normalize(scle, exprs_values = 1)
+scle <- runTSNE(scle, perplexity=10)
+plot <- plotTSNE(scle, colour_by = opt$colour_by, size_by = opt$size_by, shape_by = opt$shape_by)
+
+
+ggsave(opt$output_plot_file, plot, device="pdf")