--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/scater-plot-exprs-freq.R	Tue Sep 03 14:30:21 2019 -0400
@@ -0,0 +1,45 @@
+#!/usr/bin/env Rscript
+
+#Plots mean expression vs % of expressing cells and provides information as to the number of genes expressed in 50% and 25% of cells.
+# Load optparse we need to check inputs
+
+library(optparse)
+library(workflowscriptscommon)
+library(LoomExperiment)
+library(scater)
+
+# parse options
+
+option_list = list(
+  make_option(
+    c("-i", "--input-loom"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "A SingleCellExperiment object file in Loom format."
+  ),
+  make_option(
+    c("-o", "--output-plot-file"),
+    action = "store",
+    default = NA,
+    type = 'character',
+    help = "Path of the PDF output file to save plot to."
+  )
+)
+
+opt <- wsc_parse_args(option_list, mandatory = c('input_loom', 'output_plot_file'))
+
+# Check parameter values
+
+if ( ! file.exists(opt$input_loom)){
+  stop((paste('File', opt$input_loom, 'does not exist')))
+}
+
+
+# Input from Loom format
+
+scle <- import(opt$input_loom, format='loom', type='SingleCellLoomExperiment')
+
+#produce and save the scatter plot of reads vs genes
+plot <- plotExprsFreqVsMean(scle, controls = "is_feature_control_MT")
+ggsave(opt$output_plot_file, plot, device="pdf")