Mercurial > repos > iuc > scpipe
comparison scpipe.xml @ 1:4ec6717872b1 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/scpipe commit ffcc4ba8dcf01fd19bee6d946f9789bcf4ed680a
author | iuc |
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date | Fri, 17 Aug 2018 08:22:49 -0400 |
parents | 32e1bfc6b7b2 |
children | 5c4bca9dd4a2 |
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0:32e1bfc6b7b2 | 1:4ec6717872b1 |
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26 | 26 |
27 #if $ref_fasta.fasta_source == "history": | 27 #if $ref_fasta.fasta_source == "history": |
28 #set $fasta_name = re.sub('[^\w\-\s]', '_', str($ref_fasta.ref_fa_hist.element_identifier)) | 28 #set $fasta_name = re.sub('[^\w\-\s]', '_', str($ref_fasta.ref_fa_hist.element_identifier)) |
29 ln -s '$ref_fasta.ref_fa_hist' '$fasta_name' && | 29 ln -s '$ref_fasta.ref_fa_hist' '$fasta_name' && |
30 #else: | 30 #else: |
31 #set $fasta_name = re.sub('[^\w\-\s]', '_', str($ref_fasta.ref_fa_builtin.element_identifier)) | 31 #set $fasta_name = os.path.basename(str($ref_fasta.ref_fa_builtin.fields.path)) |
32 ln -s '$ref_fasta.ref_fa_builtin.fields.path' '$fasta_name' && | 32 ln -s '$ref_fasta.ref_fa_builtin.fields.path' '$fasta_name' && |
33 #end if | 33 #end if |
34 | 34 |
35 #set $anno_name = re.sub('[^\w\-\s]', '_', str($exons.element_identifier)) | 35 #set $anno_name = re.sub('[^\w\-\s]', '_', str($exons.element_identifier)) |
36 #set $anno_name = $anno_name + ".gff3" | 36 #set $anno_name = $anno_name + ".gff3" |
154 <param name="rscript" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="False" label="Output Rscript?" help="If this option is set to Yes, the Rscript used to annotate the IDs will be provided as a text file in the output. Default: No" /> | 154 <param name="rscript" type="boolean" truevalue="TRUE" falsevalue="FALSE" checked="False" label="Output Rscript?" help="If this option is set to Yes, the Rscript used to annotate the IDs will be provided as a text file in the output. Default: No" /> |
155 <param name="rdata" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output RData file?" | 155 <param name="rdata" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Output RData file?" |
156 help="Output all the data used by R to construct the tables and plots, can be loaded into R. Default: No"> | 156 help="Output all the data used by R to construct the tables and plots, can be loaded into R. Default: No"> |
157 </param> | 157 </param> |
158 <section name="adv" title="Advanced Options"> | 158 <section name="adv" title="Advanced Options"> |
159 <param argument="--rmlow" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Remove reads with N in barcode or UMI. Default: Yes" /> | 159 <param argument="--rmlow" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Remove reads with N in barcode or UMI" help="Default: Yes" /> |
160 <param argument="--rmN" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Remove reads with low quality. Default: Yes" /> | 160 <param argument="--rmN" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Remove reads with low quality" help="Default: Yes" /> |
161 <param argument="--minq" type="integer" min="0" value="20" label="Minimum read quality. Default: 20" /> | 161 <param argument="--minq" type="integer" min="0" value="20" label="Minimum read quality" help="Default: 20" /> |
162 <param argument="--numbq" type="integer" min="0" value="2" label="Maximum number of bases below minq. Default: 2" /> | 162 <param argument="--numbq" type="integer" min="0" value="2" label="Maximum number of bases below minq" help="Default: 2" /> |
163 <param argument="--stnd" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Perform strand-specific mapping. Default: Yes" /> | 163 <param argument="--stnd" type="boolean" truevalue="True" falsevalue="False" checked="True" label="Perform strand-specific mapping" help="Default: Yes" /> |
164 <param argument="--max_mis" type="integer" min="0" value="1" label="Maximum mismatch allowed in barcode. Default: 1" /> | 164 <param argument="--max_mis" type="integer" min="0" value="1" label="Maximum mismatch allowed in barcode" help="Default: 1" /> |
165 <param argument="--UMI_cor" type="integer" min="0" value="1" label="Correct UMI sequence error" help="0 means no correction, 1 means simple correction and merge UMI with distance 1. Default: 1" /> | 165 <param argument="--UMI_cor" type="integer" min="0" value="1" label="Correct UMI sequence error" help="0 means no correction, 1 means simple correction and merge UMI with distance 1. Default: 1" /> |
166 <param argument="--gene_fl" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Remove low abundant genes" help="Low abundant is defined as only one copy of one UMI for this gene. Default: No" /> | 166 <param argument="--gene_fl" type="boolean" truevalue="True" falsevalue="False" checked="False" label="Remove low abundant genes" help="Low abundant is defined as only one copy of one UMI for this gene. Default: No" /> |
167 <param argument="--max_reads" type="integer" min="0" value="1000000" label="Maximum reads processed" help="Maximum reads processed if detecting barcodes. Default: 1,000,000" /> | 167 <param argument="--max_reads" type="integer" min="0" value="1000000" label="Maximum reads processed" help="Maximum reads processed if detecting barcodes. Default: 1,000,000" /> |
168 <param argument="--min_count" type="integer" min="0" value="10" label="Minimum count to keep" help="Minimum count to keep if detecting barcodes. Barcode will be discarded if it has lower count. This should be set according to --max_reads. Default: 10" /> | 168 <param argument="--min_count" type="integer" min="0" value="10" label="Minimum count to keep" help="Minimum count to keep if detecting barcodes. Barcode will be discarded if it has lower count. This should be set according to --max_reads. Default: 10" /> |
169 </section> | 169 </section> |
204 <assert_contents> | 204 <assert_contents> |
205 <has_text text="scPipe report for sample" /> | 205 <has_text text="scPipe report for sample" /> |
206 </assert_contents> | 206 </assert_contents> |
207 </output> | 207 </output> |
208 </test> | 208 </test> |
209 <!-- Ensure built-in fasta works --> | |
210 <test> | |
211 <param name="fasta_source" value="cached"/> | |
212 <param name="exons" ftype="gff3" value="mm10_MT19.gff3.gz"/> | |
213 <param name="paired_format_selector" value="paired" /> | |
214 <param name="in1" ftype="fastqsanger.gz" dbkey="mm10" value="CB51_MT19_R1.gz"/> | |
215 <param name="in2" ftype="fastqsanger.gz" dbkey="mm10" value="CB51_MT19_R2.gz"/> | |
216 <param name="us" value="-1"/> | |
217 <param name="max_reads" value="5000000"/> | |
218 <param name="min_count" value="100"/> | |
219 <param name="report" value="False" /> | |
220 <output name="out_matrix" > | |
221 <assert_contents> | |
222 <has_text text="ENSMUSG00000064351" /> | |
223 </assert_contents> | |
224 </output> | |
225 </test> | |
209 </tests> | 226 </tests> |
210 <help><![CDATA[ | 227 <help><![CDATA[ |
211 .. class:: infomark | 228 .. class:: infomark |
212 | 229 |
213 **What it does** | 230 **What it does** |