Mercurial > repos > iuc > snippy
annotate snippy.xml @ 6:4df82423ba61 draft
"planemo upload commit 1235e6893a7e0d9ea3a600204c515fec1810dd93"
author | iuc |
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date | Mon, 04 Nov 2019 20:07:06 -0500 |
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1 <tool id="snippy" name="snippy" version="@VERSION@+galaxy0"> |
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2 <description> |
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3 Snippy finds SNPs between a haploid reference genome and your NGS sequence reads. |
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4 </description> |
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5 <macros> |
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6 <import>macros.xml</import> |
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7 </macros> |
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8 <expand macro="requirements" /> |
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9 <expand macro="version_command" /> |
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10 |
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11 <command detect_errors="exit_code"><![CDATA[ |
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12 |
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13 @REFERENCE_SOURCE_FILE@ |
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14 |
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15 snippy |
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16 --outdir 'out' |
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17 --cpus \${GALAXY_SLOTS:-1} |
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18 --ram \$((\${GALAXY_MEMORY_MB:-4096}/1024)) |
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19 @REFERENCE_COMMAND@ |
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20 --mapqual $adv.mapqual |
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21 --mincov $adv.mincov |
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22 --minfrac $adv.minfrac |
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23 --minqual $adv.minqual |
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24 #if $adv.rgid |
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25 --rgid '$adv.rgid' |
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26 #end if |
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27 #if $adv.bwaopt |
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28 --bwaopt '$adv.bwaopt' |
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29 #end if |
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30 |
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31 #if str( $fastq_input.fastq_input_selector ) == "paired" |
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32 --R1 '$fastq_input.fastq_input1' |
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33 --R2 '$fastq_input.fastq_input2' |
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34 #elif str( $fastq_input.fastq_input_selector ) == "paired_collection" |
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35 --R1 '$fastq_input.fastq_input.forward' |
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36 --R2 '$fastq_input.fastq_input.reverse' |
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37 #elif str( $fastq_input.fastq_input_selector ) == "single" |
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38 --se '$fastq_input.fastq_input_single' |
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39 #elif str( $fastq_input.fastq_input_selector ) == "paired_iv" |
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40 --peil '$fastq_input.fastq_input_interleaved' |
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41 #end if |
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42 |
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43 && |
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44 |
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45 #import re |
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46 #if str( $fastq_input.fastq_input_selector ) == "paired" |
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47 #set $dir_name = re.sub('[^\w_]', '_', $fastq_input.fastq_input1.element_identifier) |
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48 #elif str( $fastq_input.fastq_input_selector ) == "paired_collection" |
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49 #set $dir_name = re.sub('[^\w_]', '_', $fastq_input.fastq_input.name) |
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50 #elif str( $fastq_input.fastq_input_selector ) == "single" |
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51 #set $dir_name = re.sub('[^\w_]', '_', $fastq_input.fastq_input_single.element_identifier) |
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52 #elif str( $fastq_input.fastq_input_selector ) == "paired_iv" |
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53 #set $dir_name = re.sub('[^\w_]', '_', $fastq_input.fastq_input_interleaved.element_identifier) |
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54 #end if |
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55 |
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56 mkdir -p ${dir_name} && cp -r out/reference out/snps.tab out/snps.aligned.fa out/snps.vcf ${dir_name}/ && |
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57 tar -czf out.tgz ${dir_name} |
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58 #if "outcon" in str($outputs) and $adv.rename_cons |
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59 && sed -i 's/>.*/>${dir_name}/' out/snps.consensus.fa |
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60 #end if |
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61 |
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62 |
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63 ]]> </command> |
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64 |
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65 <inputs> |
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66 <expand macro="reference_selector" /> |
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67 <conditional name="fastq_input"> |
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68 <param name="fastq_input_selector" type="select" label="Single or Paired-end reads" help="Select between paired and single end data"> |
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69 <option value="paired">Paired</option> |
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70 <option value="single">Single</option> |
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71 <option value="paired_collection">Paired Collection</option> |
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72 <option value="paired_iv">Paired Interleaved</option> |
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73 </param> |
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74 <when value="paired"> |
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75 <param name="fastq_input1" type="data" format="fastqsanger,fasta" label="Select first set of reads" help="Specify dataset with forward reads"/> |
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76 <param name="fastq_input2" type="data" format="fastqsanger,fasta" label="Select second set of reads" help="Specify dataset with reverse reads"/> |
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77 </when> |
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78 <when value="single"> |
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79 <param name="fastq_input_single" type="data" format="fastqsanger,fasta" label="Select fastq dataset" help="Specify dataset with single reads"/> |
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80 </when> |
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81 <when value="paired_collection"> |
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82 <param name="fastq_input" format="fastqsanger,fasta" type="data_collection" collection_type="paired" label="Select a paired collection" help="See help section for an explanation of dataset collections"/> |
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83 </when> |
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84 <when value="paired_iv"> |
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85 <param name="fastq_input_interleaved" type="data" format="fastqsanger" label="Select fastq dataset" help="Specify dataset with interleaved reads"/> |
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86 </when> |
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87 </conditional> |
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88 |
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89 <section name="adv" title="Advanced parameters" expanded="false"> |
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90 <param name="mapqual" type="integer" value="60" label="Minimum mapping quality" help="Minimum mapping quality to allow" /> |
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91 <param name="mincov" type="integer" value="10" label="Minimum coverage" help="Minimum coverage to call a snp" /> |
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92 <param name="minfrac" type="float" value="0.9" label="Minumum proportion for variant evidence" help="Minumum proportion for variant evidence" /> |
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93 <param name="minqual" type="float" value="100.0" label="Minumum QUALITY in VCF column 6" help="Minumum QUALITY in VCF column 6" /> |
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94 <param name="rgid" type="text" value="" label="Bam header @RG ID" help="Use this @RG ID: in the BAM header" /> |
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95 <param name="bwaopt" type="text" value="" label="Extra BWA MEM options" help="Extra BWA MEM options, eg. -x pacbio" /> |
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96 <param name="rename_cons" type="boolean" truevalue="rename_cons" falsevalue="" help="When producing an output of the reference genome with variants instantiated, edit the header so that it is named after the input VCF" /> |
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97 </section> |
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98 |
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99 <param name="outputs" type="select" multiple="true" display="checkboxes" label="Output selection"> |
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100 <option value="outvcf" selected="True">The final annotated variants in VCF format</option> |
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101 <option value="outgff" selected="False">The variants in GFF3 format</option> |
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102 <option value="outtab" selected="True">A simple tab-separated summary of all the variants</option> |
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103 <option value="outsum" selected="False">A summary of the samples and mapping</option> |
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104 <option value="outlog" selected="False">A log file with the commands run and their outputs</option> |
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105 <option value="outaln" selected="False">A version of the reference but with - at position with depth=0 and N for 0 to depth to --mincov (does not have variants)</option> |
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106 <option value="outcon" selected="False">A version of the reference genome with all variants instantiated</option> |
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107 <option value="outbam" selected="False">The alignments in BAM format. Note that multi-mapping and unmapped reads are not present.</option> |
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108 <option value="outzip" selected="True">Zipped files needed for input into snippy-core</option> |
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109 </param> |
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110 |
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111 </inputs> |
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112 |
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113 <outputs> |
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114 |
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115 <data format="vcf" name="snpvcf" label="${tool.name} on ${on_string} snps vcf file" from_work_dir="out/snps.vcf"> |
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116 <filter>outputs and 'outvcf' in outputs</filter> |
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117 </data> |
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118 <data format="gff3" name="snpgff" label="${tool.name} on ${on_string} snps gff file" from_work_dir="out/snps.gff"> |
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119 <filter>outputs and 'outgff' in outputs</filter> |
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120 </data> |
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121 <data format="tabular" name="snptab" label="${tool.name} on ${on_string} snps table" from_work_dir="out/snps.tab"> |
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122 <filter>outputs and 'outtab' in outputs</filter> |
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123 </data> |
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124 <data format="tabular" name="snpsum" label="${tool.name} on ${on_string} snps summary" from_work_dir="out/snps.txt"> |
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125 <filter>outputs and 'outsum' in outputs</filter> |
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126 </data> |
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127 <data format="txt" name="snplog" label="${tool.name} on ${on_string} log file" from_work_dir="out/snps.log"> |
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128 <filter>outputs and 'outlog' in outputs</filter> |
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129 </data> |
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130 <data format="fasta" name="snpalign" label="${tool.name} on ${on_string} aligned fasta" from_work_dir="out/snps.aligned.fa"> |
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131 <filter>outputs and 'outaln' in outputs</filter> |
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132 </data> |
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133 <data format="fasta" name="snpconsensus" label="${tool.name} on ${on_string} consensus fasta" from_work_dir="out/snps.consensus.fa"> |
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134 <filter>outputs and 'outcon' in outputs</filter> |
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135 </data> |
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136 <data format="bam" name="snpsbam" label="${tool.name} on ${on_string} mapped reads (bam)" from_work_dir="out/snps.bam"> |
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137 <filter>outputs and 'outbam' in outputs</filter> |
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138 </data> |
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139 <data format="zip" name="outdir" label="${tool.name} on ${on_string} dir for snippy core" from_work_dir="out.tgz"> |
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140 <filter>outputs and 'outzip' in outputs</filter> |
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141 </data> |
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142 |
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143 </outputs> |
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144 |
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145 <tests> |
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146 |
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147 <test> <!-- test 0 - fasta ref no snps --> |
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148 <!-- <param name="ref" value="reference.fasta" ftype="fasta" /> --> |
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149 <conditional name="reference_source"> |
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150 <param name="reference_source_selector" value="history"/> |
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151 <param name="ref_file" value="reference.fasta" ftype="fasta"/> |
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152 </conditional> |
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153 <param name="fastq_input_selector" value="paired" /> |
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154 <param name="fastq_input1" ftype="fastqsanger" value="a_1.fastq" /> |
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155 <param name="fastq_input2" ftype="fastqsanger" value="a_2.fastq" /> |
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156 <param name="mincov" value="2" /> |
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157 <param name="minqual" value="60" /> |
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158 <param name="outputs" value="outgff,outsum" /> |
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159 <output name="snpsum" ftype="tabular" file="a_fna_ref_mincov_2_minqual_60.snps.txt" lines_diff="6" /> |
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160 <output name="snpgff" ftype="gff3" file="a_fna_ref_mincov_2_minqual_60.snps.gff" /> |
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161 </test> |
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162 |
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163 <test> <!-- test 1 - fasta ref one snp --> |
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164 <conditional name="reference_source"> |
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165 <param name="reference_source_selector" value="history"/> |
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166 <param name="ref_file" value="reference.fasta" ftype="fasta"/> |
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167 </conditional> |
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168 <param name="fastq_input_selector" value="paired" /> |
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169 <param name="fastq_input1" ftype="fastqsanger" value="b_1.fastq" /> |
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170 <param name="fastq_input2" ftype="fastqsanger" value="b_2.fastq" /> |
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171 <param name="mincov" value="2" /> |
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172 <param name="minqual" value="60" /> |
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173 <param name="outputs" value="outgff,outsum" /> |
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174 <output name="snpsum" ftype="tabular" file="b_fna_ref_mincov_2_minqual_60.snps.txt" lines_diff="6" /> |
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175 <output name="snpgff" ftype="gff3" file="b_fna_ref_mincov_2_minqual_60.snps.gff" /> |
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176 </test> |
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177 |
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178 <test> <!-- test 2 - fasta ref one snp paired_collection --> |
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179 <conditional name="reference_source"> |
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180 <param name="reference_source_selector" value="history"/> |
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181 <param name="ref_file" value="reference.fasta" ftype="fasta"/> |
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182 </conditional> |
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183 <param name="fastq_input_selector" value="paired_collection" /> |
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184 <param name="fastq_input"> |
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185 <collection type="paired"> |
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186 <element name="forward" ftype="fastqsanger" value="b_1.fastq" /> |
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187 <element name="reverse" ftype="fastqsanger" value="b_2.fastq" /> |
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188 </collection> |
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189 </param> |
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190 <param name="mincov" value="2" /> |
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191 <param name="minqual" value="60" /> |
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192 <param name="outputs" value="outgff,outsum" /> |
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193 <output name="snpsum" ftype="tabular" file="b_fna_ref_mincov_2_minqual_60.snps.txt" lines_diff="6" /> |
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194 <output name="snpgff" ftype="gff3" file="b_fna_ref_mincov_2_minqual_60.snps.gff" /> |
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195 </test> |
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196 |
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197 <test> <!-- test 3 - fasta ref one snp single --> |
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198 <conditional name="reference_source"> |
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199 <param name="reference_source_selector" value="history"/> |
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200 <param name="ref_file" value="reference.fasta" ftype="fasta"/> |
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201 </conditional> |
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202 <param name="fastq_input_selector" value="single" /> |
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203 <param name="fastq_input_single" value="b_2.fastq" ftype="fastqsanger" /> |
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204 <param name="mincov" value="2" /> |
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205 <param name="minqual" value="60" /> |
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206 <param name="outputs" value="outgff,outsum" /> |
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207 <output name="snpsum" ftype="tabular" file="b_fna_ref_mincov_2_minqual_60.snps.txt" lines_diff="6" /> |
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208 <output name="snpgff" ftype="gff3" file="b_2_fna_ref_mincov_2_minqual_60.snps.gff" /> |
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209 </test> |
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210 |
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211 <test> <!-- test 4 - reference source as cached --> |
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212 <conditional name="reference_source"> |
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213 <param name="reference_source_selector" value="cached"/> |
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214 <param name="ref_file" value="test_id"/> |
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215 </conditional> |
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216 <param name="fastq_input_selector" value="single" /> |
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217 <param name="fastq_input_single" value="b_2.fastq" ftype="fastqsanger" /> |
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218 <param name="mincov" value="2" /> |
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219 <param name="minqual" value="60" /> |
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220 <param name="outputs" value="outgff,outsum" /> |
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221 <output name="snpsum" ftype="tabular" file="b_fna_ref_mincov_2_minqual_60.snps.txt" lines_diff="6" /> |
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222 <output name="snpgff" ftype="gff3" file="b_2_fna_ref_mincov_2_minqual_60.snps.gff" /> |
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223 </test> |
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224 </tests> |
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225 |
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226 |
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227 <help><![CDATA[ |
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228 |
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229 **Snippy @VERSION@** |
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230 |
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231 Snippy finds SNPs between a haploid reference genome and your NGS sequence reads. It will find both substitutions (snps) and insertions/deletions (indels). |
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232 |
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233 **Author** |
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234 |
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235 Torsten Seemann |
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236 |
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237 **Inputs** |
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238 |
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239 - NGS Reads in fastq format (single or paired end) |
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240 - Reference file in either fasta or genbank format |
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241 |
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242 If the reference file is supplied in genbank format, snpeff will be called to determine the effect of any snps found. |
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243 |
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244 **Advanced options** |
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245 |
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246 - mapping quality - Integer - Minimum mapping quality to allow (default '60') |
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247 |
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248 - minimum coverage - Integer - Minimum coverage of variant site (default '10') |
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249 |
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250 - minimum fraction - Float - Minumum proportion for variant evidence (default '0.9') |
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251 |
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252 - minimum quality - Float - Minumum QUALITY in VCF column 6 (default '100.0') |
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253 |
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254 - rgid - String - Use this @RG ID: in the BAM header (default '') |
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255 |
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256 - bwaopt - Extra BWA MEM options, eg. -x pacbio (default '') |
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257 |
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258 **Further information** |
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259 |
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260 For a much more in depth description of snippy and how it works, see https://github.com/tseemann/snippy |
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261 |
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262 ]]> </help> |
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263 <expand macro="citations"/> |
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264 |
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265 </tool> |