<tool id="snpSift_filter" name="SnpSift Filter" version="@WRAPPER_VERSION@.1">
    <description>Filter variants using arbitrary expressions</description>
    <macros>
        <import>snpSift_macros.xml</import>
    </macros>
    <expand macro="requirements" />
    <expand macro="stdio" />
    <expand macro="version_command" />

    <command><![CDATA[
        @CONDA_SNPSIFT_JAR_PATH@ &&
        java -Xmx6G -jar "\$SNPSIFT_JAR_PATH/SnpSift.jar" filter -f "$input" -e "$exprFile" $inverse
        #if $filtering.mode == 'field':
            #if $filtering.replace.pass:
                --pass
                #if $filtering.replace.filterId and len(str($filtering.replace.filterId).strip()) > 0:
                    --filterId "$filtering.replace.filterId"
                #end if
            #end if
            #if $filtering.addFilter and len(str($filtering.addFilter).strip()) > 0:
                --addFilter "$filtering.addFilter"
            #end if
            #if $filtering.rmFilter and len(str($filtering.rmFilter).strip()) > 0:
                --rmFilter "$filtering.rmFilter"
            #end if
        #end if
         > "$output"
]]>
    </command>
    <configfiles>
        <configfile name="exprFile">
$expr#slurp
        </configfile>
    </configfiles>
    <inputs>
        <param format="vcf" name="input" type="data" label="Variant input file in VCF format"/>
        <param name="expr" type="text" label="Filter criteria" help="Need help? See below a few examples." >
            <sanitizer sanitize="False"/>
        </param>
        <param name="inverse" type="boolean" truevalue="--inverse" falsevalue="" checked="false" label="Inverse filter" help="Show lines that do not match filter expression" />
        <conditional name="filtering">
            <param name="mode" type="select" label="Filter mode">
                <option value="entries" selected="true">Retain entries that pass filter, remove other entries</option>
                <option value="field">Change the FILTER field, but retain all entries</option>
            </param>
            <when value="entries"/>
            <when value="field">
                <conditional name="replace">
                    <param name="pass" type="boolean" truevalue="yes" falsevalue="no" checked="false" label="Set matching entry FILTER to 'PASS'"
                           help="appends an ID tag to non-matching entry FILTER "/>
                    <when value="no"/>
                    <when value="yes">
                        <param name="filterId" type="text" value="" label="ID appended to non-matching (##FILTER tag in header and FILTER VCF field)." help="Default ID is 'SnpSift'"/>
                    </when>
                </conditional>
                <param name="addFilter" type="text" value="" label="Add a string to FILTER VCF field if 'expression' is true." />
                <param name="rmFilter" type="text" value="" label="Remove a string from FILTER VCF field if 'expression' is true (and 'str' is in the field)." />
            </when>
        </conditional>
    </inputs>

    <outputs>
        <data format="vcf" name="output" />
    </outputs>
    <tests>
        <test>
        <param name="input" ftype="vcf" value="test01.vcf"/>
        <param name="expr" value="QUAL >= 50"/>
        <param name="mode" value="entries"/>
        <output name="output">
            <assert_contents>
            <has_text text="28837706" />
            <not_has_text text="NT_166464" />
            </assert_contents>
        </output>
        </test>

        <test>
        <param name="input" ftype="vcf" value="test01.vcf"/>
        <param name="expr" value="(CHROM = '19')"/>
        <param name="mode" value="entries"/>
        <output name="output">
            <assert_contents>
            <has_text text="3205820" />
            <not_has_text text="NT_16" />
            </assert_contents>
        </output>
        </test>

        <test>
        <param name="input" ftype="vcf" value="test01.vcf"/>
        <param name="expr" value="(POS &gt;= 20175) &amp; (POS &lt;= 35549)"/>
        <param name="mode" value="entries"/>
        <output name="output">
            <assert_contents>
            <has_text text="20175" />
            <has_text text="35549" />
            <has_text text="22256" />
            <not_has_text text="18933" />
            <not_has_text text="37567" />
            </assert_contents>
        </output>
        </test>

        <test>
        <param name="input" ftype="vcf" value="test01.vcf"/>
        <param name="expr" value="( DP &gt;= 5 )"/>
        <param name="mode" value="entries"/>
        <output name="output">
            <assert_contents>
            <has_text text="DP=5;" />
            <has_text text="DP=6;" />
            <not_has_text text="DP=1;" />
            </assert_contents>
        </output>
        </test>
    </tests>
    <help><![CDATA[

**SnpSift filter**

You can filter a VCF file using arbitrary expressions, for instance "(QUAL > 30) | (exists INDEL) | ( countHet() > 2 )". The actual expressions can be quite complex, so it allows for a lot of flexibility.

Some examples:

  - *I want just the variants from the second million bases of chr1*:

    ::

    ( CHROM = 'chr1' ) & ( POS > 1000000 ) & ( POS < 2000000 )

  - *Filter value is either 'PASS' or it is missing*:

    ::

    (FILTER = 'PASS') | ( na FILTER )

  - *I want to filter lines with an ANN annotation EFFECT of 'frameshift_variant' ( for vcf files using Sequence Ontology terms )*:

    ::

    ( ANN[*].EFFECT has 'frameshift_variant' )

    **Important** According to the specification, there can be more than one EFFECT separated by & (e.g. 'missense_variant&splice_region_variant', thus using has operator is better than using equality operator (=). For instance 'missense_variant&splice_region_variant' = 'missense_variant' is false, whereas 'missense_variant&splice_region_variant' has 'missense_variant' is true.

  - *I want to filter lines with an EFF of 'FRAME_SHIFT' ( for vcf files using Classic Effect names )*:

    ::

    ( EFF[*].EFFECT = 'FRAME_SHIFT' )

  - *I want to filter out samples with quality less than 30*:

    ::

    ( QUAL > 30 )

  - *...but we also want InDels that have quality 20 or more*:

    ::

    (( exists INDEL ) & (QUAL >= 20)) | (QUAL >= 30 )

  - *...or any homozygous variant present in more than 3 samples*:

    ::

    (countHom() > 3) | (( exists INDEL ) & (QUAL >= 20)) | (QUAL >= 30 )

  - *...or any heterozygous sample with coverage 25 or more*:

    ::

    ((countHet() > 0) & (DP >= 25)) | (countHom() > 3) | (( exists INDEL ) & (QUAL >= 20)) | (QUAL >= 30 )

  - *I want to keep samples where the genotype for the first sample is homozygous variant and the genotype for the second sample is reference*:

    ::

    (isHom( GEN[0] ) & isVariant( GEN[0] ) & isRef( GEN[1] ))


**For information regarding HGVS and Sequence Ontology terms versus classic names**:

  - http://snpeff.sourceforge.net/SnpEff_manual.html#cmdline for the options: -classic, -hgvs, and -sequenceOntology
  - http://snpeff.sourceforge.net/SnpEff_manual.html#input for the table containing the classic name and sequence onology term for each effect


@EXTERNAL_DOCUMENTATION@
	http://snpeff.sourceforge.net/SnpSift.html#filter

]]>
    </help>
    <expand macro="citations" />
</tool>