# HG changeset patch # User iuc # Date 1660137268 0 # Node ID 48cf45a534566b31d2b696e1dcbe1e18d7a19a51 # Parent b3490b036c5a15b6edbe4bae6ec5027cdb376fca planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/spades commit 35f71aa486d8754ee6a8387659032fc7c93d1be3 diff -r b3490b036c5a -r 48cf45a53456 macros.xml --- a/macros.xml Wed Jul 13 07:41:31 2022 +0000 +++ b/macros.xml Wed Aug 10 13:14:28 2022 +0000 @@ -1,6 +1,6 @@ 3.15.4 - 1 + 2 spades @@ -43,6 +43,9 @@ + @@ -738,7 +741,7 @@ SPAdes - St. Petersburg genome assembler - is an assembly toolkit containing various assembly pipelines. `_ (e.g. assemble using k-mer lengths 21,33,55,77,99,127). However, due to increased error rate some changes of k-mer lengths (e.g. selection of shorter ones) may be required. For example, if you ran SPAdes with k-mer lengths 21,33,55,77 and then decided to assemble the same data set using more iterations and larger values of K, you can run SPAdes once again specifying the same output folder and the following options: --restart-from k77 -k 21,33,55,77,99,127 --mismatch-correction -o . Do not forget to copy contigs and scaffolds from the previous run. We're planning to tackle issue of selecting k-mer lengths for IonTorrent reads in next versions. +The selection of k-mer length is non-trivial for IonTorrent. If the dataset is more or less conventional (good coverage, not high GC, etc), then use our `recommendation for long reads `_ (e.g. assemble using k-mer lengths 21,33,55,77,99,127). However, due to increased error rate some changes of k-mer lengths (e.g. selection of shorter ones) may be required. For example, if you ran SPAdes with k-mer lengths 21,33,55,77 and then decided to assemble the same data set using more iterations and larger values of K, you can run SPAdes once again specifying the same output folder and the following options: --restart-from k77 -k 21,33,55,77,99,127 --mismatch-correction -o . Do not forget to copy contigs and scaffolds from the previous run. We're planning to tackle issue of selecting k-mer lengths for IonTorrent reads in next versions. You may need no error correction for Hi-Q enzyme at all. However, we suggest trying to assemble your data with and without error correction and select the best variant. diff -r b3490b036c5a -r 48cf45a53456 rnaviralspades.xml --- a/rnaviralspades.xml Wed Jul 13 07:41:31 2022 +0000 +++ b/rnaviralspades.xml Wed Aug 10 13:14:28 2022 +0000 @@ -18,6 +18,7 @@ @PREPROCESS_CONTIGS_FILES@ @PREPROCESS_SANGER_FILES@ +@OMP_THREADS@ ## run spades.py --rnaviral -o 'output' @@ -351,7 +352,7 @@ **References** -More information are available on `github `_ and on the `project website `_. +More information are available on `github `_. ]]>