Mercurial > repos > iuc > stacks2_clonefilter
diff stacks_clonefilter.xml @ 0:1b6d07160736 draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
author | iuc |
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date | Mon, 01 Jul 2019 10:57:08 -0400 |
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children | b75174403a65 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/stacks_clonefilter.xml Mon Jul 01 10:57:08 2019 -0400 @@ -0,0 +1,162 @@ +<tool id="stacks2_clonefilter" name="Stacks2: clone filter" profile="@PROFILE@" version="@STACKS_VERSION@+galaxy@WRAPPER_VERSION@"> +<description>Identify PCR clones</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"/> + <expand macro="version_cmd"/> + <command detect_errors="aggressive"><![CDATA[ +@FASTQ_INPUT_FUNCTIONS@ + +mkdir stacks_inputs stacks_outputs && + +#set ($link_command, $fwd_path, $rev_path, $inputype) = $fastq_input_batch($input_type.fqinputs, $input_type.input_type_select) +$link_command + +clone_filter +#if $input_type.input_type_select == 'single': + -f '$fwd_path' +#else + -1 '$fwd_path' + -2 '$rev_path' +#end if + +-i $inputype + +-o stacks_outputs +$capture +$input_type.barcode_encoding +#if $oligo_len_1 + --oligo_len_1 $oligo_len_1 +#end if +#if $oligo_len_2 + --oligo_len_2 $oligo_len_2 +#end if +$retain_oligo +## only supports fastq.gz output since the +## the program outputs empty files for fasta/fastq +-y gzfastq +@TEE_APPEND_LOG@ +@CAT_LOG_TO_STDERR@ + +## move outputs such that Galaxy can find them +#if $capture: + #if $input_type.input_type_select == "single" + && mv stacks_outputs/*.discards.fq.gz '$discarded' + #else + && mv stacks_outputs/*.discards.1.fq.gz '$discarded_pair.forward' + && mv stacks_outputs/*.discards.2.fq.gz '$discarded_pair.reverse' + #end if +#end if +#if $input_type.input_type_select == "single" + && mv stacks_outputs/*.fq.gz '$clean' +#else + && mv stacks_outputs/*.1.fq.gz '$clean_pair.forward' + && mv stacks_outputs/*.2.fq.gz '$clean_pair.reverse' +#end if +]]></command> + <inputs> + <expand macro="fastq_input_bc"/> + <param name="capture" type="boolean" checked="false" truevalue="-D" falsevalue="" argument="-D" label="Capture discarded reads to a file" /> + <param name="oligo_len_1" type="integer" value="0" label="Length of the single-end oligo sequence in dataset"/> + <param name="oligo_len_2" optional="true" type="integer" label="Length of the paired-end oligo sequence in dataset"/> + <param argument="--retain_oligo" type="boolean" checked="false" truevalue="--retain_oligo" falsevalue="" label="Do not trim off the random oligo sequence (if oligo is inline)" /> + <expand macro="in_log"/> + </inputs> + <outputs> + <expand macro="out_log"/> + <data format="fastqsanger.gz" name="clean" from_work_dir="outputs/R1.fq.gz" label="${tool.name} on ${on_string}"> + <filter>input_type['input_type_select'] == 'single'</filter> + </data> + <collection name="clean_pair" type="paired" label="${tool.name} on ${on_string}"> + <filter>input_type['input_type_select'] == 'paired'</filter> + </collection> + <data name="discarded" format="fastqsanger" label="${tool.name} on ${on_string}: discarded reads"> + <filter>capture and input_type['input_type_select'] == 'single' and not options_kmer_char['k_dist']</filter> + </data> + <collection name="discarded_pair" type="paired" label="${tool.name} on ${on_string}: discarded reads"> + <filter>capture and input_type['input_type_select'] == 'paired' and not options_kmer_char['k_dist']</filter> + </collection> + </outputs> + <tests> + <!-- single end, defaults--> + <test> + <conditional name="input_type"> + <param name="input_type_select" value="single" /> + <param name="fqinputs" ftype="fastqsanger.gz" value="clonefilter/R1_0001.1.fq.gz" /> + </conditional> + <param name="oligo_len_1" value="6" /> + <param name="add_log" value="yes" /> + <output name="output_log" ftype="txt" file="clonefilter/clonefilter.log" lines_diff="8"/> + <output name="clean" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.single.gz"/> + </test> + <!-- single end, alt BCencoding, capture--> + <test> + <conditional name="input_type"> + <param name="input_type_select" value="single" /> + <param name="fqinputs" ftype="fastqsanger.gz" value="clonefilter/R1_0001.1.fq.gz" /> + <param name="barcode_encoding" value="--index_null" /> + </conditional> + <param name="capture" value="-D" /> + <param name="oligo_len_1" value="6" /> + <assert_command> + <has_text text="-D" /> + </assert_command> + <output name="clean" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.gz"/> + <output name="discarded" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.gz"/> + </test> + <!-- paired end, defaults--> + <test> + <conditional name="input_type"> + <param name="input_type_select" value="paired" /> + <param name="fqinputs"> + <collection type="paired"> + <element name="forward" value="clonefilter/R1_0001.1.fq.gz" /> + <element name="reverse" value="clonefilter/R2_0001.2.fq.gz" /> + </collection> + </param> + </conditional> + <param name="oligo_len_1" value="6" /> + <output_collection name="clean_pair" type="paired"> + <element name="forward" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.gz" /> + <element name="reverse" compare="sim_size" file="clonefilter/Removed2_0001.2.2.fq.gz" /> + </output_collection> + </test> + <!-- paired end, non defaults --> + <test> + <conditional name="input_type"> + <param name="input_type_select" value="paired" /> + <param name="fqinputs"> + <collection type="paired"> + <element name="forward" value="clonefilter/R1_0001.1.fq.gz" /> + <element name="reverse" value="clonefilter/R2_0001.2.fq.gz" /> + </collection> + </param> + </conditional> + <param name="oligo_len_1" value="6" /> + <param name="capture" value="-D" /> + <param name="retain_oligo" value="--retain_oligo" /> + <assert_command> + <has_text text="--retain_oligo" /> + </assert_command> + <output_collection name="clean_pair" type="paired"> + <element name="forward" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.gz" /> + <element name="reverse" compare="sim_size" file="clonefilter/Removed2_0001.2.2.fq.gz" /> + </output_collection> + <output_collection name="discarded_pair" type="paired"> + <element name="forward" compare="sim_size" file="clonefilter/Removed1_0001.1.1.fq.gz" /> + <element name="reverse" compare="sim_size" file="clonefilter/Removed2_0001.2.2.fq.gz" /> + </output_collection> + </test> + </tests> + <help> +<![CDATA[ +.. class:: infomark + +The clone_filter program is designed to identify PCR clones. + +@STACKS_INFOS@ +]]> + </help> + <expand macro="citation" /> +</tool>