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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit feda4e2ea70c013fcddd1dbdeab73158fe9c86a4
author | iuc |
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date | Mon, 23 May 2022 17:49:36 +0000 |
parents | 42201b90e3f4 |
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<tool id="stacks2_clonefilter" name="Stacks2: clone filter" profile="@PROFILE@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"> <description>Identify PCR clones</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements"/> <expand macro="version_cmd"/> <command detect_errors="aggressive"><![CDATA[ @FASTQ_INPUT_FUNCTIONS@ trap ">&2 cat '$output_log'" err exit && mkdir stacks_inputs stacks_outputs && #set ($link_command, $fwd_path, $rev_path, $inputype) = $fastq_input_batch($input_type.fqinputs, $input_type.input_type_select) $link_command clone_filter #if $input_type.input_type_select == 'single': -f '$fwd_path' #else -1 '$fwd_path' -2 '$rev_path' #end if -i $inputype -o stacks_outputs $capture $input_type.barcode_encoding #if $oligo_len_1 --oligo_len_1 $oligo_len_1 #end if #if $oligo_len_2 --oligo_len_2 $oligo_len_2 #end if $retain_oligo ## only supports fastq.gz output since the ## the program outputs empty files for fasta/fastq -y gzfastq @TEE_APPEND_LOG@ ## move outputs such that Galaxy can find them #if $capture: #if $input_type.input_type_select == "single" && mv stacks_outputs/*.discards.fq.gz '$discarded' #else && mv stacks_outputs/*.discards.1.fq.gz '$discarded_pair.forward' && mv stacks_outputs/*.discards.2.fq.gz '$discarded_pair.reverse' #end if #end if #if $input_type.input_type_select == "single" && mv stacks_outputs/*.fq.gz '$clean' #else && mv stacks_outputs/*.1.fq.gz '$clean_pair.forward' && mv stacks_outputs/*.2.fq.gz '$clean_pair.reverse' #end if ]]></command> <inputs> <expand macro="fastq_input_bc"/> <param name="capture" type="boolean" checked="false" truevalue="-D" falsevalue="" argument="-D" label="Capture discarded reads to a file"/> <param name="oligo_len_1" type="integer" value="0" label="Length of the single-end oligo sequence in dataset"/> <param name="oligo_len_2" optional="true" type="integer" label="Length of the paired-end oligo sequence in dataset"/> <param argument="--retain_oligo" type="boolean" checked="false" truevalue="--retain_oligo" falsevalue="" label="Do not trim off the random oligo sequence (if oligo is inline)"/> <expand macro="in_log"/> </inputs> <outputs> <expand macro="out_log"/> <expand macro="fastq_output_filter" format="fastqsanger.gz"/> </outputs> <tests> <!-- single end, defaults--> <test expect_num_outputs="2"> <conditional name="input_type"> <param name="input_type_select" value="single"/> <param name="fqinputs" ftype="fastqsanger.gz" value="clonefilter/R1_0001.1.fq.gz"/> </conditional> <param name="oligo_len_1" value="6"/> <param name="add_log" value="yes"/> <assert_command> <has_text text="--oligo_len_1 6"/> <not_has_text text="-D"/> </assert_command> <output name="output_log" ftype="txt" file="clonefilter/clonefilter.log" lines_diff="8"/> <output name="clean" compare="diff" ftype="fastqsanger.gz" file="clonefilter/Removed1_0001.1.1.fq.single.gz"/> </test> <!-- single end, alt BCencoding, capture--> <test expect_num_outputs="3"> <conditional name="input_type"> <param name="input_type_select" value="single"/> <param name="fqinputs" ftype="fastqsanger.gz" value="clonefilter/R1_0001.1.fq.gz"/> <param name="barcode_encoding" value="--index_null"/> </conditional> <param name="capture" value="true"/> <param name="oligo_len_1" value="6"/> <assert_command> <has_text text="--oligo_len_1 6"/> <has_text text="-D"/> </assert_command> <output name="output_log" ftype="txt"><assert_contents><has_text text="5 pairs of reads input."/></assert_contents></output> <output name="clean" compare="diff" ftype="fastqsanger.gz" file="clonefilter/Removed1_0001.1.1.fq.single-alt.gz"/> <output name="discarded" compare="diff" ftype="fastqsanger.gz" file="clonefilter/Discarded1_0001.1.1.fq.single.gz"/> </test> <!-- paired end, defaults--> <test expect_num_outputs="4"> <conditional name="input_type"> <param name="input_type_select" value="paired"/> <param name="fqinputs"> <collection type="paired"> <element name="forward" value="clonefilter/R1_0001.1.fq.gz" ftype="fastqsanger.gz"/> <element name="reverse" value="clonefilter/R2_0001.2.fq.gz" ftype="fastqsanger.gz"/> </collection> </param> </conditional> <param name="oligo_len_1" value="6"/> <assert_command> <has_text text="--oligo_len_1 6"/> <not_has_text text="--oligo_len_2"/> <not_has_text text="-D"/> </assert_command> <output name="output_log" ftype="txt"><assert_contents><has_text text="4 pairs of reads input."/></assert_contents></output> <output_collection name="clean_pair" type="paired" count="2"/> </test> <!-- paired end, non defaults --> <test expect_num_outputs="7"> <conditional name="input_type"> <param name="input_type_select" value="paired"/> <param name="fqinputs"> <collection type="paired"> <element name="forward" value="clonefilter/R1_0001.1.fq.gz" ftype="fastqsanger.gz"/> <element name="reverse" value="clonefilter/R2_0001.2.fq.gz" ftype="fastqsanger.gz"/> </collection> </param> </conditional> <param name="oligo_len_1" value="6"/> <param name="oligo_len_2" value="6"/> <param name="capture" value="true"/> <param name="retain_oligo" value="true"/> <assert_command> <has_text text="--oligo_len_1 6"/> <has_text text="--oligo_len_2 6"/> <has_text text="-D"/> <has_text text="--retain_oligo"/> </assert_command> <output name="output_log" ftype="txt"><assert_contents><has_text text="4 pairs of reads input."/></assert_contents></output> <output_collection name="clean_pair" type="paired" count="2"/> <output_collection name="discarded_pair" type="paired" count="2"/> </test> </tests> <help> <![CDATA[ .. class:: infomark The clone_filter program is designed to identify PCR clones. @STACKS_INFOS@ ]]> </help> <expand macro="citation"/> </tool>