Mercurial > repos > iuc > stacks2_ustacks
annotate stacks_ustacks.xml @ 1:21d2aa544835 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
author | iuc |
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date | Mon, 30 Sep 2019 14:16:43 -0400 |
parents | 7e5a174bc201 |
children | d033e1ccb386 |
rev | line source |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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1 <tool id="stacks2_ustacks" name="Stacks2: ustacks" profile="@PROFILE@" version="@STACKS_VERSION@+galaxy@WRAPPER_VERSION@"> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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2 <description>Identify unique stacks</description> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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3 <macros> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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4 <import>macros.xml</import> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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5 </macros> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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6 <expand macro="requirements"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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7 <expand macro="version_cmd"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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8 <command detect_errors="aggressive"><![CDATA[ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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9 @FASTQ_INPUT_FUNCTIONS@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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10 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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11 mkdir stacks_inputs stacks_outputs |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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12 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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13 && |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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14 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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15 #set $ID=1 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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16 #for $sample in $input_type.fqinputs |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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17 #set ($create_links, $data_path, $name, $inputype) = $fastq_input_foo($sample, "forward", ".1") |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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18 $create_links |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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19 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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20 ustacks |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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21 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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22 -f '$data_path' |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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23 -i $ID |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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24 --name $name |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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25 -m $m |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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26 -M $M |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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27 #if $N |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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28 -N $N |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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29 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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30 -p \${GALAXY_SLOTS:-1} |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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31 -t $inputype |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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32 $R |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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33 $H |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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34 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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35 ## Assembly |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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36 $assembly_options.keep_high_cov |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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37 --high_cov_thres $assembly_options.high_cov_thres |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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38 $assembly_options.d |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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39 --max_locus_stacks $assembly_options.max_locus_stacks |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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40 #if str($assembly_options.k_len) |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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41 --k_len $assembly_options.k_len |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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42 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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43 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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44 @GAP_OPTIONS@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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45 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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46 ## snp_model |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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47 #if str( $snp_options.select_model.model_type) == "bounded" |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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48 --model_type bounded |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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49 --bound_low $snp_options.select_model.bound_low |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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50 --bound_high $snp_options.select_model.bound_high |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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51 --alpha $snp_options.select_model.alpha |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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52 #else if str( $snp_options.select_model.model_type) == "snp" |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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53 --model_type snp |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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54 --alpha $snp_options.select_model.alpha |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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55 #else |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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56 --model_type fixed |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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57 --bc_err_freq $bc_err_freq |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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58 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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59 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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60 -o stacks_outputs |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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61 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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62 @TEE_APPEND_LOG@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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63 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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64 #set $ID=$ID+1 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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65 #end for |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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66 @CAT_LOG_TO_STDERR@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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67 ## If input is in gz format, stacks will output gzipped files (no option to control this) |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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68 #if $inputype.startswith('gz') |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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69 && gunzip stacks_outputs/*.gz |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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70 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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71 ]]></command> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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72 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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73 <inputs> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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74 <expand macro="fastq_input" help="Single end data or forward reads. If a paired list is provided only the forward reads are used in ustacks"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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75 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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76 <param argument="-m" type="integer" value="3" label="Minimum depth of coverage required to create a stack"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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77 <param argument="-M" type="integer" value="2" label="Maximum distance (in nucleotides) allowed between stacks"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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78 <param argument="-N" type="integer" value="" optional="true" label="Maximum distance allowed to align secondary reads to primary stacks" help="(default: M + 2)"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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79 <param argument="-R" type="boolean" checked="false" truevalue="-R" falsevalue="" label="Retain unused reads" /> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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80 <param argument="-H" type="boolean" checked="false" truevalue="-H" falsevalue="" label="Disable calling haplotypes from secondary reads" /> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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81 <section name="assembly_options" title="SNP Model Options (ustacks options)" expanded="True"> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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82 <param argument="--keep_high_cov" type="boolean" checked="false" truevalue="--keep_high_cov" falsevalue="" label="Disable the algorithm that removes highly-repetitive stacks and nearby errors. " /> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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83 <param argument="-d" type="boolean" checked="false" truevalue="-d" falsevalue="" label="Enable the Deleveraging algorithm, used for resolving over merged tags" /> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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84 <param argument="--high_cov_thres" type="float" min="0.0" value="3.0" label="Highly-repetitive stacks threshold" help="in standard deviation units"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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85 <param argument="--max_locus_stacks" type="integer" value="3" label="Maximum number of stacks at a single de novo locus"/> |
7e5a174bc201
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86 <param argument="--k_len" type="integer" value="" min="7" max="31" optional="true" label="K-mer size for matching between alleles and loci (automatically calculated by default)"/> |
7e5a174bc201
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87 </section> |
7e5a174bc201
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88 <expand macro="gap_options"/> |
7e5a174bc201
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89 |
7e5a174bc201
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90 <!-- SNP Model options --> |
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91 <section name="snp_options" title="SNP Model Options (ustacks options)" expanded="False"> |
7e5a174bc201
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92 <expand macro="snp_options_full"/> |
7e5a174bc201
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93 </section> |
7e5a174bc201
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94 <expand macro="in_log"/> |
7e5a174bc201
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95 </inputs> |
7e5a174bc201
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96 |
7e5a174bc201
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97 <outputs> |
7e5a174bc201
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98 <expand macro="out_log"/> |
7e5a174bc201
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99 <expand macro="ustacks_outputs_macro"/> |
7e5a174bc201
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100 </outputs> |
7e5a174bc201
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101 |
7e5a174bc201
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102 <tests> |
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103 <!-- paired list, default options, test for file equality --> |
7e5a174bc201
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104 <test> |
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105 <param name="input_type|input_type_select" value="paired"/> |
7e5a174bc201
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106 <param name="input_type|fqinputs"> |
7e5a174bc201
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107 <collection type="list:paired"> |
7e5a174bc201
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108 <element name="PopA_01"> |
7e5a174bc201
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109 <collection type="paired"> |
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110 <element name="forward" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger" /> |
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111 <element name="reverse" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger"/> |
7e5a174bc201
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112 </collection> |
7e5a174bc201
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113 </element> |
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114 <element name="PopA_02"> |
7e5a174bc201
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115 <collection type="paired"> |
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116 <element name="forward" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger" /> |
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117 <element name="reverse" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
7e5a174bc201
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118 </collection> |
7e5a174bc201
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119 </element> |
7e5a174bc201
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120 </collection> |
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121 </param> |
7e5a174bc201
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122 <param name="add_log" value="yes" /> |
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123 <output name="output_log"><assert_contents><has_text text="done." /></assert_contents></output> |
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124 <output_collection name="tabs" type="list" count="6"> |
7e5a174bc201
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125 <element name="PopA_01.tags" ftype="tabular" file="ustacks/PopA_01.tags.tsv" lines_diff="2"/> |
7e5a174bc201
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126 <element name="PopA_01.snps" ftype="tabular" file="ustacks/PopA_01.snps.tsv" lines_diff="2"/> |
7e5a174bc201
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127 <element name="PopA_01.alleles" ftype="tabular" file="ustacks/PopA_01.alleles.tsv" lines_diff="2"/> |
7e5a174bc201
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128 <element name="PopA_02.tags" ftype="tabular" file="ustacks/PopA_02.tags.tsv" lines_diff="2"/> |
7e5a174bc201
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129 <element name="PopA_02.snps" ftype="tabular" file="ustacks/PopA_02.snps.tsv" lines_diff="2"/> |
7e5a174bc201
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130 <element name="PopA_02.alleles" ftype="tabular" file="ustacks/PopA_02.alleles.tsv" lines_diff="2"/> |
7e5a174bc201
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131 </output_collection> |
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132 </test> |
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133 <!-- manual selected list of elements + default args, test for file equality --> |
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134 <test> |
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135 <param name="input_type|input_type_select" value="single"/> |
7e5a174bc201
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136 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.1.fq,demultiplexed/PopA_02.1.fq" ftype="fastqsanger" /> |
7e5a174bc201
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137 <param name="add_log" value="yes" /> |
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138 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
7e5a174bc201
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139 <output_collection name="tabs" count="6"> |
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140 <element name="PopA_01.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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141 <element name="PopA_01.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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142 <element name="PopA_01.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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143 <element name="PopA_02.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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144 <element name="PopA_02.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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145 <element name="PopA_02.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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146 </output_collection> |
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147 </test> |
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148 <!-- manual selected list of elements + non-default short args, test for file presence --> |
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149 <test> |
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150 <param name="input_type|input_type_select" value="single"/> |
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151 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.1.fq,demultiplexed/PopA_02.1.fq" ftype="fastqsanger" /> |
1
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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152 <param name="m" value="2" /> |
21d2aa544835
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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153 <param name="M" value="3" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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154 <param name="N" value="4"/> |
21d2aa544835
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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155 <param name="R" value="-R" /> |
21d2aa544835
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
iuc
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156 <param name="H" value="-H" /> |
0
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157 <param name="add_log" value="yes" /> |
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158 <assert_command> |
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159 <has_text text="-m 2" /> |
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160 <has_text text="-M 3" /> |
1
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
iuc
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161 <has_text text="-N 4" /> |
0
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162 <has_text text="-R" /> |
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163 <has_text text="-H" /> |
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164 </assert_command> |
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165 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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iuc
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166 <output_collection name="tabs" count="6"> |
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167 <element name="PopA_01.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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168 <element name="PopA_01.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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169 <element name="PopA_01.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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170 <element name="PopA_02.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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171 <element name="PopA_02.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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172 <element name="PopA_02.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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173 </output_collection> |
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174 </test> |
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175 <!-- paired list, non-default model options, disabled gapped alignment, test for file presence --> |
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176 <test> |
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177 <param name="input_type|input_type_select" value="paired"/> |
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178 <param name="input_type|fqinputs"> |
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179 <collection type="list:paired"> |
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180 <element name="PopA_01"> |
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181 <collection type="paired"> |
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182 <element name="forward" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger" /> |
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183 <element name="reverse" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger"/> |
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184 </collection> |
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185 </element> |
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186 <element name="PopA_02"> |
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187 <collection type="paired"> |
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188 <element name="forward" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger" /> |
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189 <element name="reverse" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
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190 </collection> |
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191 </element> |
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192 </collection> |
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193 </param> |
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194 <conditional name="select_model"> |
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195 <param name="model_type" value="snp"/> |
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196 <param name="alpha" value="0.1"/> |
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197 <param name="bound_low" value="0.1"/> |
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198 <param name="bound_high" value="0.5"/> |
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199 <param name="bc_err_freq" value="0.1"/> |
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200 </conditional> |
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201 <param name="gapped|use_gapped" value="no" /> |
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202 <param name="add_log" value="yes" /> |
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203 <assert_command> |
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204 <has_text text="--model_type snp" /> |
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205 <has_text text="--alpha 0.1" /> |
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206 <not_has_text text="--bound_low 0.1" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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207 <not_has_text text="--bound_high 0.5" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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208 <not_has_text text="--bc_err_freq 0.1" /> |
0
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209 <has_text text="--disable-gapped" /> |
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210 </assert_command> |
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211 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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212 <output_collection name="tabs" count="6"> |
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213 <element name="PopA_01.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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214 <element name="PopA_01.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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215 <element name="PopA_01.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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216 <element name="PopA_02.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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217 <element name="PopA_02.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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218 <element name="PopA_02.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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219 </output_collection> |
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220 </test> |
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221 <!-- list of fwd reads, nondefault assembly and gapped alignment options, test for file presence --> |
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222 <test> |
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223 <param name="input_type|input_type_select" value="paired"/> |
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224 <param name="input_type|fqinputs"> |
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225 <collection type="list"> |
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226 <element name="PopA_01" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger" /> |
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227 <element name="PopA_02" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
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228 </collection> |
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229 </param> |
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230 <param name="assembly_options|keep_high_cov" value="true"/> |
0
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231 <param name="assembly_options|d" value="-d" /> |
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232 <param name="assembly_options|high_cov_thres" value="2.0"/> |
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233 <param name="assembly_options|max_locus_stacks" value="4" /> |
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234 <param name="assembly_options|k_len" value="12"/> |
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235 <param name="gapped|use_gapped" value="yes" /> |
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236 <param name="gapped|max_gaps" value="3" /> |
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237 <param name="gapped|min_aln_len" value="0.7"/> |
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238 <param name="add_log" value="yes" /> |
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239 <assert_command> |
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240 <has_text text="--keep_high_cov" /> |
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241 <has_text text="-d" /> |
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242 <has_text text="--high_cov_thres 2.0" /> |
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243 <has_text text="--max_locus_stacks 4" /> |
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244 <has_text text="--k_len 12" /> |
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245 <has_text text="--max_gaps 3" /> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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246 <has_text text="--min_aln_len 0.7" /> |
0
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247 </assert_command> |
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248 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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249 <output_collection name="tabs" count="6"> |
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250 <element name="PopA_01.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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251 <element name="PopA_01.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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252 <element name="PopA_01.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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253 <element name="PopA_02.tags"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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254 <element name="PopA_02.snps"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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255 <element name="PopA_02.alleles"><assert_contents><has_text text="generated on " /></assert_contents></element> |
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256 </output_collection> |
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257 </test> |
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258 </tests> |
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259 |
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260 <help> |
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261 <![CDATA[ |
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262 .. class:: infomark |
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263 |
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264 **What it does** |
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265 |
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266 The unique stacks program will take as input a set of short-read sequences and align them into exactly-matching stacks. Comparing the stacks it will form a set of loci and detect SNPs at each locus using a maximum likelihood framework |
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267 |
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268 -------- |
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269 |
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270 **Input files** |
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271 |
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272 One or more FASTQ, FASTA files. Can be given by selecting them manually, as dataset list, or paired dataset list. Note, for the latter ustacks only uses the forward reads -- reverse reads are incorporated in tsv2bam. |
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273 |
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274 **Output files** |
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275 |
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276 - XXX.tags.tsv file: |
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277 |
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278 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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279 |
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280 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence. |
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281 |
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282 |
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283 - XXX.snps.tsv file: |
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284 |
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285 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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286 |
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287 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one. |
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288 |
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289 |
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290 - XXX.alleles.tsv file: |
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291 |
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292 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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293 |
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294 @STACKS_INFOS@ |
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295 ]]> |
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296 </help> |
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297 <expand macro="citation" /> |
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298 </tool> |