Mercurial > repos > iuc > stacks2_ustacks
annotate stacks_ustacks.xml @ 7:912f904a6e1e draft default tip
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit feda4e2ea70c013fcddd1dbdeab73158fe9c86a4
author | iuc |
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date | Mon, 23 May 2022 17:56:22 +0000 |
parents | bb0475c884ff |
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rev | line source |
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d1fda11e602f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 16654f3b3faaba994cea5fda8d4907ea4f059605"
iuc
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1 <tool id="stacks2_ustacks" name="Stacks2: ustacks" profile="@PROFILE@" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@"> |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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2 <description>Identify unique stacks</description> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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3 <macros> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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4 <import>macros.xml</import> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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5 </macros> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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6 <expand macro="requirements"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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7 <expand macro="version_cmd"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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8 <command detect_errors="aggressive"><![CDATA[ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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9 @FASTQ_INPUT_FUNCTIONS@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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10 |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
iuc
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11 trap ">&2 cat '$output_log'" err exit && |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
iuc
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12 mkdir stacks_inputs stacks_outputs && |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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13 |
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bb0475c884ff
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 2f4c9bfc48d63075ae18a1687e8d01ffea509084
iuc
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14 #set $ID=int($processing_options.i) |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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15 #for $sample in $input_type.fqinputs |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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16 #set ($create_links, $data_path, $name, $inputype) = $fastq_input_foo($sample, "forward", ".1") |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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17 $create_links |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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18 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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19 ustacks |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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20 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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21 -f '$data_path' |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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22 -i $ID |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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23 --name $name |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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24 -m $m |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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25 -M $M |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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26 #if $N |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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27 -N $N |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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28 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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29 -p \${GALAXY_SLOTS:-1} |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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30 -t $inputype |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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31 $R |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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32 $H |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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33 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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34 ## Assembly |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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35 $assembly_options.keep_high_cov |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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36 --high_cov_thres $assembly_options.high_cov_thres |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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37 $assembly_options.d |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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38 --max_locus_stacks $assembly_options.max_locus_stacks |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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39 #if str($assembly_options.k_len) |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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40 --k_len $assembly_options.k_len |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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41 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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42 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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43 @GAP_OPTIONS@ |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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44 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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45 ## snp_model |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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46 #if str( $snp_options.select_model.model_type) == "bounded" |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
parents:
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47 --model_type bounded |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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48 --bound_low $snp_options.select_model.bound_low |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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49 --bound_high $snp_options.select_model.bound_high |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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50 --alpha $snp_options.select_model.alpha |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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51 #else if str( $snp_options.select_model.model_type) == "snp" |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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52 --model_type snp |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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53 --alpha $snp_options.select_model.alpha |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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54 #else |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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55 --model_type fixed |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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56 --bc_err_freq $bc_err_freq |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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57 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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58 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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59 -o stacks_outputs |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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60 @TEE_APPEND_LOG@ |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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61 && |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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62 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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63 #set $ID=$ID+1 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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64 #end for |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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65 true |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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66 ## If input is in gz format, stacks will output gzipped files (no option to control this) |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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67 #if $inputype.startswith('gz') |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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68 && gunzip stacks_outputs/*.gz |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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69 #end if |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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70 ]]></command> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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71 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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72 <inputs> |
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d1fda11e602f
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 16654f3b3faaba994cea5fda8d4907ea4f059605"
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73 <expand macro="fastq_input" multiple="true" listtype="list:paired" help="Single end data or forward reads. If a paired list is provided only the forward reads are used in ustacks"/> |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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74 |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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75 <param argument="-m" type="integer" value="3" label="Minimum depth of coverage required to create a stack"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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76 <param argument="-M" type="integer" value="2" label="Maximum distance (in nucleotides) allowed between stacks"/> |
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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77 <param argument="-N" type="integer" value="" optional="true" label="Maximum distance allowed to align secondary reads to primary stacks" help="(default: M + 2)"/> |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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78 <param argument="-R" type="boolean" checked="false" truevalue="-R" falsevalue="" label="Retain unused reads"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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79 <param argument="-H" type="boolean" checked="false" truevalue="-H" falsevalue="" label="Disable calling haplotypes from secondary reads"/> |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
iuc
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80 <section name="assembly_options" title="SNP Model Options (ustacks options)" expanded="True"> |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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81 <param argument="--keep_high_cov" type="boolean" checked="false" truevalue="--keep_high_cov" falsevalue="" label="Disable the algorithm that removes highly-repetitive stacks and nearby errors. "/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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82 <param argument="-d" type="boolean" checked="false" truevalue="-d" falsevalue="" label="Enable the Deleveraging algorithm, used for resolving over merged tags"/> |
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7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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83 <param argument="--high_cov_thres" type="float" min="0.0" value="3.0" label="Highly-repetitive stacks threshold" help="in standard deviation units"/> |
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84 <param argument="--max_locus_stacks" type="integer" value="3" label="Maximum number of stacks at a single de novo locus"/> |
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85 <param argument="--k_len" type="integer" value="" min="7" max="31" optional="true" label="K-mer size for matching between alleles and loci (automatically calculated by default)"/> |
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86 </section> |
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87 <expand macro="gap_options"/> |
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88 |
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89 <!-- SNP Model options --> |
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90 <section name="snp_options" title="SNP Model Options (ustacks options)" expanded="False"> |
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91 <expand macro="snp_options_full"/> |
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92 </section> |
6
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93 <section name="processing_options" title="Processing options" expanded="False"> |
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94 <param argument="-i" type="integer" value="1" label="Start identifier at" help="If you are combining multiple ustacks runs at the cstacks stage, use this option to avoid having different samples with the same identifier."/> |
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95 </section> |
0
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96 <expand macro="in_log"/> |
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97 </inputs> |
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98 |
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99 <outputs> |
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100 <expand macro="out_log"/> |
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101 <expand macro="ustacks_outputs_macro"/> |
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102 </outputs> |
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103 |
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104 <tests> |
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105 <!-- paired list, default options, test for file equality --> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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106 <test expect_num_outputs="2"> |
0
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107 <param name="input_type|input_type_select" value="paired"/> |
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108 <param name="input_type|fqinputs"> |
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109 <collection type="list:paired"> |
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110 <element name="PopA_01"> |
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111 <collection type="paired"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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112 <element name="forward" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger"/> |
0
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113 <element name="reverse" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger"/> |
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114 </collection> |
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115 </element> |
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116 <element name="PopA_02"> |
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117 <collection type="paired"> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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118 <element name="forward" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
0
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119 <element name="reverse" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
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120 </collection> |
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121 </element> |
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122 </collection> |
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123 </param> |
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124 <param name="add_log" value="yes"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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125 <output name="output_log"><assert_contents><has_text text="done."/></assert_contents></output> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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126 <!-- 1st test checks for file content allowing differences in the 2 comment lines that contain date and version --> |
0
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127 <output_collection name="tabs" type="list" count="6"> |
6
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128 <element name="PopA_01.tags"> |
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129 <assert_contents> |
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130 <has_text text="# ustacks version" /> |
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131 </assert_contents> |
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132 </element> |
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133 <element name="PopA_01.snps"> |
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134 <assert_contents> |
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135 <has_text text="# ustacks version" /> |
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136 </assert_contents> |
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137 </element> |
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138 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
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139 <element name="PopA_02.tags"> |
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140 <assert_contents> |
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141 <has_text text="# ustacks version" /> |
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142 </assert_contents> |
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143 </element> |
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144 <element name="PopA_02.snps"> |
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145 <assert_contents> |
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146 <has_text text="# ustacks version" /> |
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147 </assert_contents> |
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148 </element> |
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149 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
0
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150 </output_collection> |
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151 </test> |
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152 <!-- manual selected list of elements + default args, test for file equality --> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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153 <test expect_num_outputs="2"> |
0
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154 <param name="input_type|input_type_select" value="single"/> |
2
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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155 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.1.fq,demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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156 <param name="add_log" value="yes"/> |
0
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157 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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158 <output_collection name="tabs" count="6"> |
6
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159 <expand macro="test_element_stacks_completed" element_name="PopA_01.tags" /> |
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160 <expand macro="test_element_stacks_completed" element_name="PopA_01.snps" /> |
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161 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
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162 <expand macro="test_element_stacks_completed" element_name="PopA_02.tags" /> |
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163 <expand macro="test_element_stacks_completed" element_name="PopA_02.snps" /> |
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164 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
0
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165 </output_collection> |
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166 </test> |
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167 <!-- manual selected list of elements + non-default short args, test for file presence --> |
2
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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168 <test expect_num_outputs="2"> |
0
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169 <param name="input_type|input_type_select" value="single"/> |
2
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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170 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.1.fq,demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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171 <param name="m" value="2"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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172 <param name="M" value="3"/> |
1
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit c300b84615660459bb0020fa74ccd3b874d329a4"
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173 <param name="N" value="4"/> |
2
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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174 <param name="R" value="-R"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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175 <param name="H" value="-H"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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176 <param name="add_log" value="yes"/> |
0
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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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177 <assert_command> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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178 <has_text text="-m 2"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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179 <has_text text="-M 3"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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180 <has_text text="-N 4"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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181 <has_text text="-R"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
iuc
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182 <has_text text="-H"/> |
0
7e5a174bc201
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit b395fa36fa826e26085820ba3a9faacaeddcb460
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183 </assert_command> |
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184 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
7e5a174bc201
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185 <output_collection name="tabs" count="6"> |
6
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186 <expand macro="test_element_stacks_completed" element_name="PopA_01.tags" /> |
bb0475c884ff
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187 <expand macro="test_element_stacks_completed" element_name="PopA_01.snps" /> |
bb0475c884ff
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 2f4c9bfc48d63075ae18a1687e8d01ffea509084
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188 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
bb0475c884ff
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189 <expand macro="test_element_stacks_completed" element_name="PopA_02.tags" /> |
bb0475c884ff
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 2f4c9bfc48d63075ae18a1687e8d01ffea509084
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190 <expand macro="test_element_stacks_completed" element_name="PopA_02.snps" /> |
bb0475c884ff
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit 2f4c9bfc48d63075ae18a1687e8d01ffea509084
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191 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
0
7e5a174bc201
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192 </output_collection> |
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193 </test> |
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194 <!-- paired list, non-default model options, disabled gapped alignment, test for file presence --> |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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195 <test expect_num_outputs="2"> |
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196 <param name="input_type|input_type_select" value="paired"/> |
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197 <param name="input_type|fqinputs"> |
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198 <collection type="list:paired"> |
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199 <element name="PopA_01"> |
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200 <collection type="paired"> |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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201 <element name="forward" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger"/> |
0
7e5a174bc201
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202 <element name="reverse" value="demultiplexed/PopA_01.2.fq" ftype="fastqsanger"/> |
7e5a174bc201
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203 </collection> |
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204 </element> |
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205 <element name="PopA_02"> |
7e5a174bc201
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206 <collection type="paired"> |
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d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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207 <element name="forward" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
0
7e5a174bc201
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208 <element name="reverse" value="demultiplexed/PopA_02.2.fq" ftype="fastqsanger"/> |
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209 </collection> |
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210 </element> |
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211 </collection> |
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212 </param> |
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213 <conditional name="select_model"> |
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214 <param name="model_type" value="snp"/> |
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215 <param name="alpha" value="0.1"/> |
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216 <param name="bound_low" value="0.1"/> |
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217 <param name="bound_high" value="0.5"/> |
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218 <param name="bc_err_freq" value="0.1"/> |
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219 </conditional> |
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220 <param name="gapped|use_gapped" value="no"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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221 <param name="add_log" value="yes"/> |
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222 <assert_command> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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223 <has_text text="--model_type snp"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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224 <has_text text="--alpha 0.1"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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225 <not_has_text text="--bound_low 0.1"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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226 <not_has_text text="--bound_high 0.5"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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227 <not_has_text text="--bc_err_freq 0.1"/> |
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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228 <has_text text="--disable-gapped"/> |
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229 </assert_command> |
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230 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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231 <output_collection name="tabs" count="6"> |
6
bb0475c884ff
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232 <expand macro="test_element_stacks_completed" element_name="PopA_01.tags" /> |
bb0475c884ff
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233 <expand macro="test_element_stacks_completed" element_name="PopA_01.snps" /> |
bb0475c884ff
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234 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
bb0475c884ff
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235 <expand macro="test_element_stacks_completed" element_name="PopA_02.tags" /> |
bb0475c884ff
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236 <expand macro="test_element_stacks_completed" element_name="PopA_02.snps" /> |
bb0475c884ff
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237 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
0
7e5a174bc201
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238 </output_collection> |
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239 </test> |
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240 <!-- list of fwd reads, nondefault assembly and gapped alignment options, test for file presence --> |
2
d033e1ccb386
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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241 <test expect_num_outputs="2"> |
0
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242 <param name="input_type|input_type_select" value="paired"/> |
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243 <param name="input_type|fqinputs"> |
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244 <collection type="list"> |
2
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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245 <element name="PopA_01" value="demultiplexed/PopA_01.1.fq" ftype="fastqsanger"/> |
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246 <element name="PopA_02" value="demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
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247 </collection> |
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248 </param> |
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249 <param name="assembly_options|keep_high_cov" value="true"/> |
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250 <param name="assembly_options|d" value="-d"/> |
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251 <param name="assembly_options|high_cov_thres" value="2.0"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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252 <param name="assembly_options|max_locus_stacks" value="4"/> |
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253 <param name="assembly_options|k_len" value="12"/> |
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254 <param name="gapped|use_gapped" value="yes"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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255 <param name="gapped|max_gaps" value="3"/> |
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256 <param name="gapped|min_aln_len" value="0.7"/> |
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257 <param name="add_log" value="yes"/> |
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258 <assert_command> |
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259 <has_text text="--keep_high_cov"/> |
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260 <has_text text="-d"/> |
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261 <has_text text="--high_cov_thres 2.0"/> |
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262 <has_text text="--max_locus_stacks 4"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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263 <has_text text="--k_len 12"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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264 <has_text text="--max_gaps 3"/> |
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"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks2 commit f55e2407891a3c1f73f14a77b7ddadcd6f5eb1f8"
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265 <has_text text="--min_aln_len 0.7"/> |
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266 </assert_command> |
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267 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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268 <output_collection name="tabs" count="6"> |
6
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269 <expand macro="test_element_stacks_completed" element_name="PopA_01.tags" /> |
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270 <expand macro="test_element_stacks_completed" element_name="PopA_01.snps" /> |
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271 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
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272 <expand macro="test_element_stacks_completed" element_name="PopA_02.tags" /> |
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273 <expand macro="test_element_stacks_completed" element_name="PopA_02.snps" /> |
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274 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
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275 </output_collection> |
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276 </test> |
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277 <!-- test setting i --> |
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278 <test expect_num_outputs="2"> |
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279 <param name="input_type|input_type_select" value="single"/> |
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280 <param name="input_type|fqinputs" value="demultiplexed/PopA_01.1.fq,demultiplexed/PopA_02.1.fq" ftype="fastqsanger"/> |
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281 <param name="add_log" value="yes"/> |
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282 <param name="i" value="42"/> |
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283 <output name="output_log" ftype="txt"><assert_contents><has_text text="ustacks is done."/></assert_contents></output> |
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284 <output_collection name="tabs" count="6"> |
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285 <expand macro="test_element_stacks_completed" element_name="PopA_01.tags" /> |
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286 <expand macro="test_element_stacks_completed" element_name="PopA_01.snps" /> |
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287 <expand macro="test_element_stacks_completed" element_name="PopA_01.alleles" /> |
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288 <expand macro="test_element_stacks_completed" element_name="PopA_02.tags" /> |
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289 <expand macro="test_element_stacks_completed" element_name="PopA_02.snps" /> |
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290 <expand macro="test_element_stacks_completed" element_name="PopA_02.alleles" /> |
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291 </output_collection> |
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292 </test> |
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293 |
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294 </tests> |
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295 |
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296 <help> |
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297 <![CDATA[ |
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298 .. class:: infomark |
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299 |
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300 **What it does** |
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301 |
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302 The unique stacks program will take as input a set of short-read sequences and align them into exactly-matching stacks. Comparing the stacks it will form a set of loci and detect SNPs at each locus using a maximum likelihood framework |
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303 |
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304 -------- |
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305 |
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306 **Input files** |
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307 |
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308 One or more FASTQ, FASTA files. Can be given by selecting them manually, as dataset list, or paired dataset list. Note, for the latter ustacks only uses the forward reads -- reverse reads are incorporated in tsv2bam. |
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309 |
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310 **Output files** |
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311 |
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312 - XXX.tags.tsv file: |
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313 |
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314 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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315 |
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316 Notes: For the tags file, each stack will start in the file with a consensus sequence for the entire stack followed by the flags for that stack. Then, each individual read that was merged into that stack will follow. The next stack will start with another consensus sequence. |
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317 |
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318 |
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319 - XXX.snps.tsv file: |
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320 |
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321 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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322 |
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323 Notes: If a stack has two SNPs called within it, then there will be two lines in this file listing each one. |
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324 |
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325 |
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326 - XXX.alleles.tsv file: |
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327 |
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328 See `Stacks output description <http://catchenlab.life.illinois.edu/stacks/manual/#files>`_ |
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329 |
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330 @STACKS_INFOS@ |
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331 ]]> |
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332 </help> |
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333 <expand macro="citation"/> |
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334 </tool> |