Mercurial > repos > iuc > stacks_assembleperead

annotate stacks_assembleperead.xml @ 13:38ecfb8d52c6 draft default tip

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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stacks commit 74ee33c6e30744a6da8deb7116d431d80ee80edb
author iuc
date Fri, 07 Apr 2023 22:02:47 +0000
parents 6eaf0f9836c8
children
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1 <tool id="stacks_assembleperead" name="Stacks: assemble read pairs by locus" version="@WRAPPER_VERSION@.1">
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2 <description>run the STACKS sort_read_pairs.pl and exec_velvet.pl wrappers</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="bio_tools"/>
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7 <expand macro="requirements" />
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8 <expand macro="stdio"/>
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9 <command><![CDATA[
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11 mkdir stacks_inputs reads stacks_outputs
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12 &&
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13 #for $input_file in $stacks_col
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14 #set $ext = ""
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15 #if not str($input_file.element_identifier).endswith('.tsv')
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16 #set $ext = ".tsv"
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17 #end if
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18 ln -s '${input_file}' 'stacks_inputs/${input_file.element_identifier}${ext}' &&
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19 #end for
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21 #for $input_file in $reads
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22 #set $name = str($input_file.element_identifier)
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23 ## sort_read_pairs is expecting strange fastq names: <sample_name>.fq_2
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24 #if $name.endswith('.1.fq')
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25 ## handle a common case
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26 #set $name = $name[:-5]+".fq_1"
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27 #else if $name.endswith('.2.fq')
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28 ## handle a common case
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29 #set $name = $name[:-5]+".fq_2"
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30 #else if not $name.endswith('.fq') and not $name.endswith('.fq_2')
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31 ## no extension, consider it's a fq_2 file
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32 #set $name = $name + ".fq_2"
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33 #end if
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34 ln -s '${input_file}' 'reads/${name}' &&
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35 #end for
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37 sort_read_pairs.pl
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38 -p stacks_inputs
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39 -s 'reads'
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40
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41 #if $whitelist
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42 -w '$whitelist'
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43 #end if
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44
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45 #if $threshold
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46 -r $threshold
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47 #end if
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48
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49 -o stacks_outputs
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50
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51 #if $velvet.use_velvet == "yes"
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52 ## remove possible empty files
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53 && find stacks_outputs -type f -size 0 -exec rm {} \;
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54
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55 &&
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56 mkdir assembled
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57 &&
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58 velvet_path=`which velveth` && velvet_path=`dirname "\$velvet_path"`
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59 &&
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60 exec_velvet.pl -s stacks_outputs -o assembled -c -M ${velvet.contig_length} -e "\$velvet_path"
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61 #end if
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62
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63 ]]></command>
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64 <inputs>
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65 <param name="stacks_col" argument="-p" format="tabular,txt" type="data_collection" collection_type="list" label="Output from previous Stacks pipeline steps (e.g. denovo_map or refmap)" />
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66 <param name="reads" argument="-s" format="fastqsanger" type="data" multiple="true" label="Files containing reads to assemble" help="only R2 reads" />
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67
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68 <param name="whitelist" argument="-w" format="txt,tabular" type="data" optional="true" label="White list of catalog IDs to include" />
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69 <param name="threshold" argument="-r" type="integer" value="" optional="true" label="Minimum number of reads by locus"/>
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70
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71 <conditional name="velvet">
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72 <param name="use_velvet" type="select" label="Perform assembly with Velvet" help="If not selected, the tool will only produce of collection of fasta files (one per locus) containing reads ready to assemble.">
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73 <option value="no" selected="true">No</option>
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74 <option value="yes">Yes</option>
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75 </param>
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76 <when value="no"/>
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77 <when value="yes">
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78 <param name="contig_length" type="integer" value="200" label="Minimum length for asssembled contigs"/>
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79 </when>
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80 </conditional>
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81 </inputs>
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82 <outputs>
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83 <collection name="collated" type="list" label="Collated FASTA files per locus on ${on_string}">
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84 <filter>velvet['use_velvet'] == "no"</filter>
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85 <discover_datasets pattern="(?P&lt;name&gt;.+)\.fa(sta)?$" ext="fasta" directory="stacks_outputs" />
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86 </collection>
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87
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88 <data format="fasta" name="contigs" label="Assembled contigs on ${on_string}" from_work_dir="assembled/collated.fa">
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89 <filter>velvet['use_velvet'] == "yes"</filter>
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90 </data>
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91 </outputs>
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92
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93 <tests>
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94 <test>
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95 <param name="stacks_col">
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96 <collection type="list">
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97 <element name="batch_1.catalog.alleles.tsv" ftype="tabular" value="genotypes/batch_1.catalog.alleles.tsv" />
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98 <element name="batch_1.catalog.snps.tsv" ftype="tabular" value="genotypes/batch_1.catalog.snps.tsv" />
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99 <element name="batch_1.catalog.tags.tsv" ftype="tabular" value="genotypes/batch_1.catalog.tags.tsv" />
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100 <element name="PopA_01.alleles.tsv" ftype="tabular" value="genotypes/PopA_01.alleles.tsv" />
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101 <element name="PopA_01.matches.tsv" ftype="tabular" value="genotypes/PopA_01.matches.tsv" />
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102 <element name="PopA_01.snps.tsv" ftype="tabular" value="genotypes/PopA_01.snps.tsv" />
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103 <element name="PopA_01.tags.tsv" ftype="tabular" value="genotypes/PopA_01.tags.tsv" />
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104 <element name="PopA_02.alleles.tsv" ftype="tabular" value="genotypes/PopA_02.alleles.tsv" />
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105 <element name="PopA_02.matches.tsv" ftype="tabular" value="genotypes/PopA_02.matches.tsv" />
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106 <element name="PopA_02.snps.tsv" ftype="tabular" value="genotypes/PopA_02.snps.tsv" />
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107 <element name="PopA_02.tags.tsv" ftype="tabular" value="genotypes/PopA_02.tags.tsv" />
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108 </collection>
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109 </param>
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110 <param name="reads" value="demultiplexed/PopA_01.2.fq,demultiplexed/PopA_02.2.fq" ftype="fastqsanger" />
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111
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112 <output_collection name="collated">
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113 <element name="1">
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114 <assert_contents>
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115 <has_text text="CCGATCAGCATCAGTAGTTTTCAACGAGCTGGCCCAATGGTGTATAACTATGTGGTAGAGAGAAACTGCTGCTATCACTCACGATATAAGCCCTCTGACG" />
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116 </assert_contents>
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117 </element>
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118 </output_collection>
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119 </test>
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120 <test>
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121 <param name="stacks_col">
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122 <collection type="list">
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123 <element name="batch_1.catalog.alleles.tsv" ftype="tabular" value="genotypes/batch_1.catalog.alleles.tsv" />
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124 <element name="batch_1.catalog.snps.tsv" ftype="tabular" value="genotypes/batch_1.catalog.snps.tsv" />
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125 <element name="batch_1.catalog.tags.tsv" ftype="tabular" value="genotypes/batch_1.catalog.tags.tsv" />
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126 <element name="PopA_01.alleles.tsv" ftype="tabular" value="genotypes/PopA_01.alleles.tsv" />
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127 <element name="PopA_01.matches.tsv" ftype="tabular" value="genotypes/PopA_01.matches.tsv" />
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128 <element name="PopA_01.snps.tsv" ftype="tabular" value="genotypes/PopA_01.snps.tsv" />
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129 <element name="PopA_01.tags.tsv" ftype="tabular" value="genotypes/PopA_01.tags.tsv" />
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130 <element name="PopA_02.alleles.tsv" ftype="tabular" value="genotypes/PopA_02.alleles.tsv" />
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131 <element name="PopA_02.matches.tsv" ftype="tabular" value="genotypes/PopA_02.matches.tsv" />
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132 <element name="PopA_02.snps.tsv" ftype="tabular" value="genotypes/PopA_02.snps.tsv" />
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133 <element name="PopA_02.tags.tsv" ftype="tabular" value="genotypes/PopA_02.tags.tsv" />
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134 </collection>
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135 </param>
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136 <param name="reads" value="demultiplexed/PopA_01.2.fq,demultiplexed/PopA_02.2.fq" ftype="fastqsanger" />
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137 <param name="velvet|use_velvet" value="yes" />
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138 <param name="velvet|contig_length" value="20" />
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139
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140 <output name="contigs">
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141 <assert_contents>
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142 <has_text text="|NODE_" />
0
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143 </assert_contents>
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144 </output>
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145 </test>
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146 </tests>
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147
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148 <help>
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149 <![CDATA[
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150 .. class:: infomark
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151
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152 **What it does**
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153
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154 This program will run each of the Stacks sort_read_pairs.pl and exec_velvet.pl utilities to assemble pair-end reads from STACKS pipeline results
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155
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156 --------
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157
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158 **Input file**
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159
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160 Output from denovo_map or ref_map
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161
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162
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163 **Output file**
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164
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165 A collated.fa file containing assembled contigs for each locus
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166
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167 @STACKS_INFOS@
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168 ]]>
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169 </help>
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170 <expand macro="citation" />
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171 </tool>