Mercurial > repos > iuc > stringtie
comparison stringtie.xml @ 17:1ebd14235b92 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/stringtie commit afa107c23e8eb889f0ed3469e2c897447be6bbf8"
author | iuc |
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date | Tue, 25 Feb 2020 18:07:47 -0500 |
parents | eba36e001f45 |
children | 258d696dbd7e |
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16:eba36e001f45 | 17:1ebd14235b92 |
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19 ln -s '$guide.guide_source.ref_builtin.fields.path' guide.gff && | 19 ln -s '$guide.guide_source.ref_builtin.fields.path' guide.gff && |
20 #end if | 20 #end if |
21 #end if | 21 #end if |
22 | 22 |
23 #if $input_bam.metadata.ftype == 'sam': | 23 #if $input_bam.metadata.ftype == 'sam': |
24 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_bam' | stringtie | 24 samtools sort -@ \${GALAXY_SLOTS:-1} '$input_bam' -T "\${TMPDIR:-.}" | stringtie |
25 #else | 25 #else |
26 stringtie '$input_bam' | 26 stringtie '$input_bam' |
27 #end if | 27 #end if |
28 | |
29 $long_reads | |
28 | 30 |
29 -o '$output_gtf' | 31 -o '$output_gtf' |
30 -p "\${GALAXY_SLOTS:-1}" | 32 -p "\${GALAXY_SLOTS:-1}" |
31 | 33 |
32 $rna_strandness | 34 $rna_strandness |
108 #end if | 110 #end if |
109 #end if | 111 #end if |
110 ]]></command> | 112 ]]></command> |
111 <inputs> | 113 <inputs> |
112 <param name="input_bam" type="data" format="sam,bam" label="Input mapped reads" help="Input BAM/SAM file containing reads you want to assemble into transcripts"/> | 114 <param name="input_bam" type="data" format="sam,bam" label="Input mapped reads" help="Input BAM/SAM file containing reads you want to assemble into transcripts"/> |
115 <param name="long_reads" argument="-L" type="boolean" truevalue="-L" falsevalue="" checked="false" label="Input contains long reads?" help="Select if the input contains long error-prone reads, e.g. from Oxford Nanopore or PacBio sequencing."/> | |
113 <param name="rna_strandness" type="select" label="Specify strand information" | 116 <param name="rna_strandness" type="select" label="Specify strand information" |
114 help="Select 'Forward (FR)' if your reads are from a forward-stranded library, 'Reverse (RF)' if your reads are from a reverse-stranded library, or 'Unstranded' if your reads are not from a stranded library. See Help section below for more information. Default: Unstranded"> | 117 help="Select 'Forward (FR)' if your reads are from a forward-stranded library, 'Reverse (RF)' if your reads are from a reverse-stranded library, or 'Unstranded' if your reads are not from a stranded library. See Help section below for more information. Default: Unstranded"> |
115 <option value="" selected="true">Unstranded</option> | 118 <option value="" selected="true">Unstranded</option> |
116 <option value="--fr">Forward (FR)</option> | 119 <option value="--fr">Forward (FR)</option> |
117 <option value="--rf">Reverse (RF)</option> | 120 <option value="--rf">Reverse (RF)</option> |