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date Mon, 28 Apr 2025 07:17:59 +0000
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1 # SyRI Galaxy wrapper
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3 Galaxy tool wrapper for [SyRI](https://github.com/schneebergerlab/syri): Synteny and Rearrangement Identifier. Syri compares alignments between two chromosome-level assemblies and identifies synteny and structural rearrangements.
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5 ## Minimap2 Galaxy Wrapper Compatibility
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7 * When using minimap2 for generating alignments for syri, the minimum required Galaxy wrapper version is `2.28+galaxy1`
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8 * This version correctly adds the `--eqx` flag, which is required by SyRI to interpret alignment CIGAR strings
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10 ## Accepted Alignment Inputs
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12 * For now alignments in `bam`, `sam` and `paf` are available as inputs
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13 * Delta file from mummer is currently not accepted
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15 ## Sample name in output vcf
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17 * Users can add a sample name to add to output vcf. This is checked by regex
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19 ```
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20 <param argument="--samplename" name="sample_name" type="text" label="Sample name for the output VCF file. (default: sample)" optional="true">
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21 <validator type="regex" message="Invalid characters in sample name">^[a-zA-Z0-9\-_]+$</validator>
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22 </param>
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23 ```
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25 ## Empty Map IDs Output — Not an Error
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27 * The output **Map IDs file** lists corresponding chromosomes between the reference and query genomes
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28 * SyRI only generates the output when the chromosome names differ between the two genomes.
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29 * If the chromosome names are identical, the file will be **absent or empty** — this is expected and not an error.
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31 ## Exit codes
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33 * Any non zero
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34 * Regex pattern `- ERROR -` in stderr/stdout