Mercurial > repos > iuc > tbprofiler
comparison tb_profiler_profile.xml @ 4:c2656b729ee9 draft
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/blob/master/tools/tb-profiler commit a51548708ea975c0a7b135a2cbaa5d094f3c5602"
author | iuc |
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date | Tue, 15 Oct 2019 14:35:47 -0400 |
parents | 19793eecc9f0 |
children | 4272bd430a01 |
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3:19793eecc9f0 | 4:c2656b729ee9 |
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39 --bam input.bam | 39 --bam input.bam |
40 #end if | 40 #end if |
41 | 41 |
42 --threads "\${GALAXY_SLOTS:-1}" | 42 --threads "\${GALAXY_SLOTS:-1}" |
43 #if $advanced.options == 'yes' | 43 #if $advanced.options == 'yes' |
44 '${advanced.call_whole_genome}' | |
44 --call_method '${advanced.call_method}' | 45 --call_method '${advanced.call_method}' |
45 --min_gene_frac '${advanced.min_gene_frac}' | 46 --min_gene_frac '${advanced.min_gene_frac}' |
46 --mapper '${advanced.mapper}' | 47 --mapper '${advanced.mapper}' |
47 --min_depth '${advanced.min_depth}' | 48 --min_depth '${advanced.min_depth}' |
48 --af '${min_allel_freq}' | 49 --af '${min_allel_freq}' |
56 #end if | 57 #end if |
57 && mv results/tbprofiler.results.json $results_json | 58 && mv results/tbprofiler.results.json $results_json |
58 #if str($fastq_or_bam.input_select) != "bam" | 59 #if str($fastq_or_bam.input_select) != "bam" |
59 && mv bam/tbprofiler.bam '${output_bam}' | 60 && mv bam/tbprofiler.bam '${output_bam}' |
60 #end if | 61 #end if |
61 && bcftools view -Ov -o'${output_vcf}' vcf/tbprofiler.targets.csq.bcf | 62 && bcftools view -Ov -o'${output_vcf}' vcf/tbprofiler.targets.csq.vcf.gz |
62 #if $output_format == "pdf" | 63 #if $output_format == "pdf" |
63 && mv results/tbprofiler.results.pdf '${output_pdf}' | 64 && mv results/tbprofiler.results.pdf '${output_pdf}' |
64 #else if $output_format == "txt" | 65 #else if $output_format == "txt" |
65 && mv results/tbprofiler.results.txt '${output_txt}' | 66 && mv results/tbprofiler.results.txt '${output_txt}' |
66 #end if | 67 #end if |
107 <param label="Quality required for calls to be accepted" type="select" argument="--call_method"> | 108 <param label="Quality required for calls to be accepted" type="select" argument="--call_method"> |
108 <option value="low" selected="true">Low</option> | 109 <option value="low" selected="true">Low</option> |
109 <option value="high">High</option> | 110 <option value="high">High</option> |
110 <option value="optimise">Optimise</option> | 111 <option value="optimise">Optimise</option> |
111 </param> | 112 </param> |
113 <param label="Call variants on the whole genome" type="boolean" argument="--call_whole_genome" | |
114 truevalue="--call_whole_genome" falsevalue="" help="Call variants on whole genome (Useful if you need to use whole genome variants later)" /> | |
112 <param label="Minimum coverage fraction to infer deletion" type="float" help="Used to infer a deletion if the fraction of a gene covered falls below this value." argument="--min_gene_frac" value="0.9" /> | 115 <param label="Minimum coverage fraction to infer deletion" type="float" help="Used to infer a deletion if the fraction of a gene covered falls below this value." argument="--min_gene_frac" value="0.9" /> |
113 | 116 |
114 <param name="min_depth" label="Min Depth" type="integer" value="10" help="Minimum depth required to call variant. Bases with depth below this cutoff will be marked as missing (default: 10)"/> | 117 <param name="min_depth" label="Min Depth" type="integer" value="10" help="Minimum depth required to call variant. Bases with depth below this cutoff will be marked as missing (default: 10)"/> |
115 <param name="mapper" label="Mapper" type="select" help="Mapping tools to use (default: bwa)"> | 118 <param name="mapper" label="Mapper" type="select" help="Mapping tools to use (default: bwa)"> |
116 <option value="bwa" selected="true">bwa</option> | 119 <option value="bwa" selected="true">bwa</option> |
171 </tests> | 174 </tests> |
172 <help><![CDATA[ | 175 <help><![CDATA[ |
173 Summary | 176 Summary |
174 ======= | 177 ======= |
175 | 178 |
176 The pipeline aligns reads to the H37Rv reference using bowtie2, BWA or minimap2 and then calls variants using SAMtools. These variants are then compared to a drug-resistance database. We also predict the number of reads supporting drug resistance variants as an insight into hetero-resistance (not applicable for minION data). | 179 The pipeline aligns reads to the H37Rv reference using bowtie2, BWA or minimap2 and then calls variants using GATK. These variants are then compared to a drug-resistance database. We also predict the number of reads supporting drug resistance variants as an insight into hetero-resistance (not applicable for minION data). |
177 | 180 |
178 Produces a JSON output file by default. | 181 Produces a JSON output file by default. |
179 | 182 |
180 ]]> </help> | 183 ]]> </help> |
181 <citations> | 184 <citations> |