Mercurial > repos > iuc > tracy_basecall
comparison tracy_basecall.xml @ 0:f2e0d83186c7 draft default tip
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/tracy commit 8a1c82789c6ef97008ecf8f55e060422fd72f217"
author | iuc |
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date | Tue, 12 Oct 2021 14:21:34 +0000 |
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-1:000000000000 | 0:f2e0d83186c7 |
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1 <tool id="tracy_basecall" name="tracy Basecall" version="@TOOL_VERSION@+galaxy0" profile="20.09"> | |
2 <description>from Sanger chromatogram tracefile</description> | |
3 <macros> | |
4 <import>macros.xml</import> | |
5 </macros> | |
6 <expand macro="requirements" /> | |
7 <expand macro="version_command" /> | |
8 <command detect_errors="exit_code"><![CDATA[ | |
9 tracy basecall --pratio $pratio --format $format --output '$output' '$tracefile' | |
10 ]]></command> | |
11 <inputs> | |
12 <param name="tracefile" type="data" format="ab1,scf" label="Chromatogram tracefile" /> | |
13 <param argument="--pratio" type="float" label="Peak ratio to call a base" value="0.33" min="0" /> | |
14 <param argument="--format" type="select" label="Output format"> | |
15 <option value="fasta" selected="true">FASTA</option> | |
16 <option value="fastq">FASTQ</option> | |
17 <option value="tsv">TSV (tabular)</option> | |
18 <option value="json">JSON</option> | |
19 </param> | |
20 </inputs> | |
21 <outputs> | |
22 <data name="output" format="fasta" label="tracy basecall on ${on_string}"> | |
23 <change_format> | |
24 <when input="format" value="fasta" format="fasta"/> | |
25 <when input="format" value="fastq" format="fastq"/> | |
26 <when input="format" value="tsv" format="tabular"/> | |
27 <when input="format" value="json" format="json" /> | |
28 </change_format> | |
29 </data> | |
30 </outputs> | |
31 <tests> | |
32 <test> | |
33 <param name="tracefile" value="input1.ab1" ftype="ab1" /> | |
34 <output name="output" file="output1.fasta" ftype="fasta" /> | |
35 </test> | |
36 <test> | |
37 <param name="tracefile" value="input1.ab1" ftype="ab1" /> | |
38 <param name="format" value="json" /> | |
39 <output name="output" file="output1.json" ftype="json" /> | |
40 </test> | |
41 <test> | |
42 <param name="tracefile" value="input1.ab1" ftype="ab1" /> | |
43 <param name="pratio" value="0.2" /> | |
44 <output name="output" file="output2.fasta" ftype="fasta" /> | |
45 </test> | |
46 <test> | |
47 <param name="tracefile" value="input2.scf" ftype="scf" /> | |
48 <param name="format" value="fasta" /> | |
49 <output name="output" file="output3.fasta" ftype="fasta" /> | |
50 </test> | |
51 </tests> | |
52 <help><![CDATA[ | |
53 **What it does** | |
54 | |
55 Basecall a chromatogram trace file (using tracy_) and output the primary sequence (highest peak) in FASTQ or FASTA format. If FASTQ | |
56 format is used, the quality scores are Sanger quality scores. | |
57 | |
58 @pratio@ | |
59 | |
60 Read more here_ | |
61 | |
62 .. _tracy: https://github.com/gear-genomics/tracy | |
63 .. _here: https://www.gear-genomics.com/docs/tracy/cli/#basecalling-a-chromatogram-trace-file | |
64 | |
65 ]]></help> | |
66 <expand macro="citations" /> | |
67 </tool> |