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planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit bc4ea54f0deec4ddec8e6cf79fd547491e165686
author iuc
date Mon, 28 Aug 2017 16:53:46 -0400
parents e65e640e6196
children 199aa6821ca5
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1 <tool id="trinity" name="Trinity" version="@WRAPPER_VERSION@.0">
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2 <description>de novo assembly of RNA-Seq data</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="aggressive"><![CDATA[
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8 Trinity --no_version_check
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10 ## Inputs.
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11 #if $inputs.paired_or_single == "paired":
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13 --left ${ ','.join(['"%s"' % x for x in $inputs.left_input]) }
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15 --right ${ ','.join(['"%s"' % x for x in $inputs.right_input]) }
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17 #if $inputs.left_input[0].is_of_type('fasta'):
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18 --seqType fa
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19 #else:
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20 --seqType fq
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21 #end if
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23 #if $inputs.strand.is_strand_specific:
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24 --SS_lib_type $inputs.strand.library_type
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25 #end if
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27 $inputs.jaccard_clip
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29 #else:
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30 --single ${ ','.join(['"%s"' % x for x in $inputs.input]) }
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32 #if $inputs.input[0].is_of_type('fasta'):
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33 --seqType fa
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34 #else:
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35 --seqType fq
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36 #end if
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0
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38 #if $inputs.strand.is_strand_specific:
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39 --SS_lib_type $inputs.strand.library_type
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40 #end if
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41 #end if
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43 $norm
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45 ## Additional parameters.
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46 #if $additional_params.min_contig_length:
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47 --min_contig_length $additional_params.min_contig_length
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48 #end if
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49 #if $additional_params.long_reads:
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50 --long_reads $additional_params.long_reads
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51 #end if
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52 #if $additional_params.guided.is_guided == "yes":
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53 --genome_guided_bam $additional_params.guided.genome_guided_bam
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55 #if $additional_params.guided.genome_guided_min_coverage:
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56 --genome_guided_min_coverage $additional_params.guided.genome_guided_min_coverage
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57 #end if
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59 #if $additional_params.guided.genome_guided_min_reads_per_partition:
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60 --genome_guided_min_reads_per_partition $additional_params.guided.genome_guided_min_reads_per_partition
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61 #end if
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62
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63 #end if
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64
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65 #if $additional_params.min_kmer_cov:
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66 --min_kmer_cov $additional_params.min_kmer_cov
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67 #end if
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68
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69 ## CPU and butterfly options.
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70 --CPU \${GALAXY_SLOTS:-4} --max_memory \${TRINITY_MAX_MEMORY:-1G} --bflyHeapSpaceMax \${TRINITY_MAX_MEMORY:-1G} --bfly_opts '-V 10 --stderr'
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71
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72 ## > $trinity_log 2>&1
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73 ]]></command>
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74 <inputs>
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75 <conditional name="inputs">
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76 <param name="paired_or_single" type="select" label="Paired or Single-end data?">
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77 <option value="paired">Paired</option>
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78 <option value="single">Single</option>
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79 </param>
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80 <when value="paired">
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81 <param format="fasta,fastqsanger" argument="--left" name="left_input" multiple="true" type="data" label="Left/Forward strand reads" help=""/>
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82 <param format="fasta,fastqsanger" argument="--right" name="right_input" multiple="true" type="data" label="Right/Reverse strand reads" help=""/>
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83 <conditional name="strand">
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84 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/>
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85 <when value="false">
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86 </when>
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87 <when value="true">
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88 <param name="library_type" argument="--SS_lib_type" type="select" label="Strand-specific Library Type">
0
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89 <option value="FR">Forward-Reverse</option>
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90 <option value="RF">Reverse-Forward</option>
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91 </param>
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92 </when>
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93 </conditional>
10
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94 <param name="jaccard_clip" argument="--jaccard_clip" type="boolean" truevalue="--jaccard_clip" falsevalue="" checked="false" label="Jaccard Clip options" help="set if you expect high gene density with UTR overlap"/>
0
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95 </when>
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96 <when value="single">
10
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97 <param format="fasta,fastqsanger" name="input" argument="--single" multiple="true" type="data" label="Single-end reads" help=""/>
0
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98 <conditional name="strand">
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99 <param name="is_strand_specific" type="boolean" checked="false" label="Strand specific data"/>
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100 <when value="false">
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101 </when>
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102 <when value="true">
10
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103 <param name="library_type" argument="--SS_lib_type" type="select" label="Strand-specific Library Type">
0
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104 <option value="F">F</option>
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105 <option value="R">R</option>
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106 </param>
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107 </when>
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108 </conditional>
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109 </when>
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110 </conditional>
3
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111
10
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112 <param name="norm" type="boolean" argument="--normalize_reads" truevalue="--normalize_reads" falsevalue="" checked="true" label="Run in silico normalization of reads" help="Defaults to max. read coverage of 50."/>
0
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113
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114 <section name="additional_params" title="Additional Options" expanded="False">
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115 <param name="min_contig_length" argument="--min_contig_length" type="integer" optional="true" value="200" min="1" label="Minimum Contig Length" help="All contigs shorter than this will be discarded"/>
3
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116
0
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117 <conditional name="guided">
1
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118 <param name="is_guided" type="select" label="Use the genome guided mode?" help="If you already mapped the reads to the genome, Trinity can use this information">
0
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119 <option value="no">No</option>
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120 <option value="yes">Yes</option>
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121 </param>
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122 <when value="no">
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123 </when>
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124 <when value="yes">
10
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125 <param format="bam" name="genome_guided_bam" argument="--genome_guided_bam" type="data" label="Coordinate-sorted BAM file" />
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126 <param name="genome_guided_min_coverage" argument="--genome_guided_min_coverage" type="integer" optional="true" value="1" min="1" label="Minimum read coverage for identifying an expressed region of the genome"/>
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127 <param name="genome_guided_min_reads_per_partition" argument="--genome_guided_min_reads_per_partition" type="integer" optional="true" value="10" min="1" label="Minimum number of reads per partition"/>
0
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128 </when>
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129 </conditional>
3
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130
10
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131 <param format="fasta" name="long_reads" argument="--long_reads" type="data" optional="true" label="Error-corrected or circular consensus (CCS) pac bio reads" help="Experimental feature! Long reads must be in the same orientation as short reads if they are strand specific"/>
3
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132
10
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133 <param name="min_kmer_cov" argument="--min_kmer_cov" type="integer" optional="true" value="1" min="1" label="Minimum count for K-mers to be assembled"/>
0
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134 </section>
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135 </inputs>
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136 <outputs>
2
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137 <!--data format="txt" name="trinity_log" label="${tool.name} on ${on_string}: log" /-->
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138 <data format="fasta" name="assembled_transcripts" label="${tool.name} on ${on_string}: Assembled Transcripts" from_work_dir="trinity_out_dir/Trinity.fasta"/>
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139 </outputs>
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140 <tests>
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141 <test>
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142 <param name="paired_or_single" value="paired"/>
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143 <param name="left_input" value="reads.left.fq" ftype="fastqsanger"/>
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144 <param name="right_input" value="reads.right.fq" ftype="fastqsanger"/>
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145 <param name="is_strand_specific" value="true"/>
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146 <param name="norm" value="false"/>
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147 <param name="library_type" value="RF"/>
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148 <output name="assembled_transcripts" file="raw/Trinity.fasta" compare="sim_size" delta="500" />
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149 </test>
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150 <test>
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151 <param name="paired_or_single" value="paired"/>
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152 <param name="left_input" value="reads.left.fq" ftype="fastqsanger"/>
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153 <param name="right_input" value="reads.right.fq" ftype="fastqsanger"/>
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154 <param name="is_strand_specific" value="true"/>
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155 <param name="norm" value="true"/>
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156 <param name="library_type" value="RF"/>
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157 <output name="assembled_transcripts" file="norm/Trinity.fasta" compare="sim_size" delta="500" />
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158 </test>
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159 </tests>
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160 <help>
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161 Trinity_ assembles transcript sequences from Illumina RNA-Seq data.
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162
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163 .. _Trinity: http://trinityrnaseq.github.io
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164 </help>
3
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165
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166 <expand macro="citation" />
0
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167 </tool>