Mercurial > repos > iuc > trinity_contig_exn50_statistic
diff contig_exn50_statistic.xml @ 0:1e8a2c87461a draft
planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trinity commit ed037d83415937b11a45a4611584e71e99ad1da0
author | iuc |
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date | Thu, 23 Mar 2017 10:18:11 -0400 |
parents | |
children | 570f2b1f3cd9 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/contig_exn50_statistic.xml Thu Mar 23 10:18:11 2017 -0400 @@ -0,0 +1,44 @@ +<tool id="trinity_contig_exn50_statistic" name="Compute contig Ex90N50 statistic and Ex90 transcript count" version="@WRAPPER_VERSION@.0"> + <description>from a Trinity assembly</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements"> + </expand> + <expand macro="stdio"/> + <command><![CDATA[ + + contig_ExN50_statistic.pl + '$matrix' + '$transcripts' + > '$output' + + ]]></command> + <inputs> + <param format="tabular" name="matrix" type="data" label="Expression matrix" help="Matrix of normalized expression values produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool"/> + <param format="fasta" name="transcripts" type="data" label="Transcripts" help="De novo assembly of RNA-Seq data"/> + </inputs> + <outputs> + <data format="tabular" name="output" label="${tool.name} on ${on_string}: ExN50 statistics"/> + </outputs> + <tests> + <test> + <param name="matrix" value="count/contig_exn50_statistic/matrix.TMM.EXPR.matrix"/> + <param name="transcripts" value="count/contig_exn50_statistic/Trinity.fasta"/> + <output name="output" file="count/contig_exn50_statistic/ExN50.stats"/> + </test> + </tests> + <help> +<![CDATA[ +Trinity_ assembles transcript sequences from Illumina RNA-Seq data. +This tool computes the N50 statistic limited to the top most highly expressed transcripts that represent x% of the total normalized expression data. This requires that you have first performed transcript abundance estimation with 'Align reads and estimate abundance for a de novo assembly of RNA-Seq data by Trinity' tool and that you have built the expression matrix with 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool. + +**Inputs** + +It takes as input a transcriptome assembled with Trinity and the matrix of normalized expression values produced by 'Build expression matrix for a de novo assembly of RNA-Seq data by Trinity' tool. + +.. _Trinity: http://trinityrnaseq.github.io +]]> + </help> + <expand macro="citation" /> +</tool>