Mercurial > repos > iuc > trycycler_reconcile_msa

annotate trycycler_reconcile_msa.xml @ 0:4ac81df20c74 draft

Find changesets by keywords (author, files, the commit message), revision number or hash, or revset expression.
"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
author iuc
date Thu, 11 Feb 2021 19:26:05 +0000
parents
children b64696992ee9
Ignore whitespace changes - Everywhere: Within whitespace: At end of lines:
rev   line source
0
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
1 <tool id="trycycler_reconcile_msa" name="Trycycler reconcile/msa" version="@TOOL_VERSION@" profile="21.01">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
2 <description>reconcile the contigs within each cluster and perform a multiple sequence alignment</description>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
3 <macros>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
4 <import>macros.xml</import>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
5 </macros>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
6 <expand macro="edam_ontology"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
7 <expand macro="requirements"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
8 <version_command>trycycler --version</version_command>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
9 <command detect_errors="exit_code"><![CDATA[
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
10 #import re
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
11 #set $name = re.sub('[^\w\-\.]', '_', str($input_cluster.element_identifier))
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
12 #set $folder = $name.strip(".fasta")
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
13 #set $fullpath = "/".join(["selected_cluster",$folder])
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
14 mkdir -p "${fullpath}/1_contigs" &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
15 ln -s "${input_cluster}" "selected_cluster/${name}" &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
16 python3 "$__tool_directory__"/trycycler.py reconcile "selected_cluster/${name}" &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
17 trycycler reconcile --cluster_dir "${fullpath}"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
18 --reads $reads
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
19 #if $linear
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
20 --linear
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
21 #end if
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
22 --max_mash_dist $initial_ckeck.max_mash_dist
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
23 --max_length_diff $initial_ckeck.max_length_diff
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
24 --max_add_seq $circularisation.max_add_seq
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
25 --max_add_seq_percent $circularisation.max_add_seq_percent
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
26 --max_trim_seq $circularisation.max_trim_seq
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
27 --max_trim_seq_percent $circularisation.max_trim_seq_percent
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
28 --min_identity $final_check.min_identity
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
29 --max_indel_size $final_check.max_indel_size
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
30 --threads \${GALAXY_SLOTS:-2} &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
31 trycycler msa --cluster_dir "$fullpath"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
32 --kmer $msa.kmer
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
33 --step $msa.step
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
34 --lookahead $msa.lookahead
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
35 --threads \${GALAXY_SLOTS:-2} &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
36 mv '${fullpath}/2_all_seqs.fasta' '$reconciled_cluster' &&
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
37 mv '${fullpath}/3_msa.fasta' '$aligned_cluster'
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
38 ]]></command>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
39 <inputs>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
40 <param name="input_cluster" type="data"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
41 format="fasta" label="Cluster multi-FASTA dataset"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
42 help="The input should be an independent cluster generated by the *trycycler cluster* tool" />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
43 <param name="reads" type="data"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
44 format="fastq,fastq.gz" label="Long-read datasets"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
45 help="Long reads (FASTQ format) used to generate the assemblies" />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
46 <param argument="--linear" type="boolean"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
47 truevalue="--linear" falsevalue=""
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
48 label="Input contigs are not circular"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
49 help="Use this option if your input contigs are not circular. It will disable the circularisation-correction steps in Trycycler reconcile." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
50 <section name='initial_ckeck'
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
51 title='Reconcile initial check options'
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
52 expanded='true'>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
53 <param argument="--max_mash_dist" type="float" min="0" max="0.2"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
54 value="0.02" label="Max Mash distance"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
55 help="If any of the sequences have a pairwise Mash distance of more than this (default = 0.02), then the contigs will fail the initial check." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
56 <param argument="--max_length_diff" type="float" min="1" max="2"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
57 value="1.1" label="Max relative length factor"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
58 help="If any of the sequences have a pairwise relative length factor of more than this, then the contigs will fail the initial check. For example, if set to 1.1 (the default), then no contig can be more than 10% longer than any other." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
59 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
60 <section name="circularisation"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
61 title="Reconcile circularization options"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
62 expanded='true'>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
63 <param argument="--max_add_seq" type="integer" min="0" max="4000"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
64 value="1000" label="Max number of pb to add to circularize"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
65 help="If they are set to 1000, then Trycycler will be willing to add up to 1000 bp to circularise it. Any contig which requires more than 1000 bp added to circularise will cause Trycycler reconcile to fail." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
66 <param argument="--max_add_seq_percent" type="integer" min="0" max="10"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
67 value="5" label="Max percentage of a contig length to add to circularize"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
68 help="If they are set to 5, then Trycycler will be willing to add up to 5% of a contig's length to circularise it. Any contig which requires more than 5% of its length added to circularise will cause Trycycler reconcile to fail." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
69 <param argument="--max_trim_seq" type="integer" min="0" max="100000"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
70 value="50000" label="Max number of pb to trim to circularize"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
71 help="If they are set to 50000, then Trycycler will be willing to remove up to 5000 bp to circularise it. Any contig which requires more than 5000 bp removed to circularise will cause Trycycler reconcile to fail." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
72 <param argument="--max_trim_seq_percent" type="integer" min="0" max="20"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
73 value="10" label="Max percentage of a contig length to trim to circularize"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
74 help="If they are set to 10, then Trycycler will be willing to remove up to 10% of a contig's length to circularise it. Any contig which requires more than 10% of its length removed to circularise will cause Trycycler reconcile to fail." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
75 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
76 <section name="final_check"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
77 title="Reconcile final check"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
78 expanded="true">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
79 <param argument="--min_identity" type="integer" min="70" max="100"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
80 value="98" label="Min global alignment percentage identity"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
81 help="If any of the sequences have a pairwise global alignment percent identity of less than this (default = 98), then the contigs will fail the final check." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
82 <param argument="--max_indel_size" type="integer" min="100" max="400"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
83 value="250" label="Max alignment indel size"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
84 help="If any of the sequences have a pairwise alignment indel size of more than this (default = 250), then the contigs will fail the final check." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
85 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
86 <section name="msa"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
87 title="Multiple sequence alignment (MSA) options"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
88 expanded="true">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
89 <param argument="--kmer" type="integer" min="20" max="45"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
90 value="32" label="k-mer size"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
91 help="The k-mer size used for sequence partitioning (default = 32)" />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
92 <param argument="--step" type="integer" min="500" max="1500"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
93 value="1000" label="Step size"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
94 help="The step size used for sequence partitioning (default = 1000)." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
95 <param argument="--lookahead" type="integer" min="500" max="1500"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
96 value="1000" label="Look-ahead margin"
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
97 help="The look-ahead margin used for sequence partitioning (default = 10000)." />
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
98 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
99
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
100 </inputs>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
101 <outputs>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
102 <data name="reconciled_cluster" format="fasta" label="Trycycler reconcile on ${input_cluster.element_identifier}" from_work_dir="selected_cluster"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
103 <data name="aligned_cluster" format="fasta" label="Trycycler msa on ${input_cluster.element_identifier}" from_work_dir="selected_clusters"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
104 </outputs>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
105 <tests>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
106 <test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
107 <param name='input_cluster' value='cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
108 <param name="reads" value="reads.fastq.gz"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
109 <output name='reconciled_cluster' file='reconciled_cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
110 <output name='aligned_cluster' file='aligned_cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
111 </test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
112 <test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
113 <param name='input_cluster' value='cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
114 <param name="reads" value="reads.fastq.gz"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
115 <section name="initial_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
116 <param name="max_mash_dist" value="0.3"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
117 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
118 <section name="circularisation">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
119 <param name="max_add_seq_percent" value="7"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
120 <param name="max_trim_seq" value="47000"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
121 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
122 <section name="final_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
123 <param name="max_indel_size" value="230"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
124 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
125 <section name="msa">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
126 <param name="kmer" value="30"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
127 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
128 <output name='reconciled_cluster' file='reconciled_cluster_02.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
129 <output name='aligned_cluster' file='aligned_cluster_02.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
130 </test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
131 <test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
132 <param name='input_cluster' value='cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
133 <param name="reads" value="reads.fastq.gz"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
134 <section name="initial_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
135 <param name="max_mash_dist" value="0.3"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
136 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
137 <section name="circularisation">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
138 <param name="max_add_seq" value="900"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
139 <param name="max_trim_seq" value="45000"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
140 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
141 <section name="final_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
142 <param name="min_identity" value="97"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
143 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
144 <section name="msa">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
145 <param name="step" value="1100"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
146 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
147 <output name='reconciled_cluster' file='reconciled_cluster_03.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
148 <output name='aligned_cluster' file='aligned_cluster_03.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
149 </test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
150 <test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
151 <param name='input_cluster' value='cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
152 <param name="reads" value="reads.fastq.gz"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
153 <section name="initial_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
154 <param name="max_length_diff" value="1.2"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
155 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
156 <section name="circularisation">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
157 <param name="max_add_seq" value="920"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
158 <param name="max_trim_seq_percent" value="12"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
159 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
160 <section name="final_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
161 <param name="min_identity" value="95"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
162 <param name="max_indel_size" value="230"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
163 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
164 <section name="msa">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
165 <param name="kmer" value="33"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
166 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
167 <output name='reconciled_cluster' file='reconciled_cluster_04.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
168 <output name='aligned_cluster' file='aligned_cluster_04.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
169 </test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
170 <test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
171 <param name='input_cluster' value='cluster_01.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
172 <param name="reads" value="reads.fastq.gz"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
173 <section name="initial_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
174 <param name="max_mash_dist" value="0.3"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
175 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
176 <section name="circularisation">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
177 <param name="max_add_seq_percentage" value="8"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
178 <param name="max_trim_seq" value="45300"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
179 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
180 <section name="final_check">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
181 <param name="min_identity" value="97"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
182 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
183 <section name="msa">
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
184 <param name="step" value="1100"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
185 <param name="lookahead" value="980"/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
186 </section>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
187 <output name='reconciled_cluster' file='reconciled_cluster_05.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
188 <output name='aligned_cluster' file='aligned_cluster_05.fasta'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
189 </test>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
190
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
191 </tests>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
192 <help><![CDATA[
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
193
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
194 .. class:: infomark
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
195
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
196 **Purpose**
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
197
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
198 This tool integrates two Trycycle commands: **Trycycler reconcile** and **Trycycler msa**.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
199
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
200 The **Trycycler reconcile** tool carries out four routines:
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
201
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
202 ::
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
203
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
204 * Perform an initial check to make sure the contigs look sufficiently similar to each other.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
205 * Ensure that all contig sequences are on the same strand.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
206 * If the replicon is circular it fixes any circularisation issues (i.e. add/remove sequence at each contig's start/end as necessary)
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
207 * Perform a final alignment check to make sure the normalised/circularised contigs are sufficiently similar to each other for the next step
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
208
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
209 After that, **Trycycler msa** takes the reconciled contig sequences and runs a multiple sequence alignment.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
210
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
211 ----
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
212
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
213 .. class:: infomark
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
214
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
215 **Input**
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
216
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
217 This tool requires the clustered contings generated by the **Trycycle cluster** tool, as well as the long-read dataset with **Trycycler cluster**.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
218
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
219
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
220 ----
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
221
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
222 .. class:: infomark
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
223
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
224 **Output**
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
225
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
226 **Trycycler reconcile/msa** generates two datasets:
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
227
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
228 ::
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
229
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
230 * A multi-FASTA file dataset for each contig ready for multiple sequence alignment.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
231 * A FASTA-formatted multiple sequence alignment for each contig ready for use in generating a consensus.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
232
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
233 ----
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
234
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
235 .. class:: infomark
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
236
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
237 **Manual intervention**
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
238
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
239 Trycycler reconcile may not complete successfully, in which case you will have to intervene and run it again. Often this simply means excluding whichever contig is causing the problem, usually due to significant length differences between contigs, or particularly bad pairwise identity or large insertion/deletion. It can be done by using the **Filter sequences by length** tool.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
240
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
241 Throwing out troublesome contigs at this step is normal. To prepare a set of reduduntant assemblies aims to prevent that losing one or two would not be a problem.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
242
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
243 You should aim to have around four to eight contigs left after running Trycycler reconcile. Less than that (two or three) will not provide as many variants for the next steps and may affect your consensus sequence quality. More than that (nine or more) is fine but probably won't be of any extra benefit. If you have too few contigs for your cluster, you might want to consider going back to the start of the pipeline and generating more input assemblies. If you have plenty of contigs, you can delete some of the worst ones and run Trycycler reconcile again. Use the final check to guide you: delete the contigs with the lowest identities and largest indels relative to the other contigs.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
244
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
245 Unlike in previous steps of **Trycycler**, the msa step should be hands-off. I.e. no manual intervention is required – just run it and wait for it to finish.
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
246
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
247
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
248 ----
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
249
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
250 .. class:: infomark
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
251
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
252 @PIPELINE@
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
253 ]]></help>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
254 <expand macro='citations'/>
4ac81df20c74 "planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/trycycler commit 9d7c4277b0f96aacd466f2d497e08edcca3fa238"
iuc
parents:
diff changeset
255 </tool>