varvamp: varvamp.xml annotate

annotate varvamp.xml @ 2:eb5dcbebd242 draft

planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 2d380eea5900d7650c5ddfe8cf8d19d2554d0031

author	iuc
date	Fri, 26 Apr 2024 13:06:36 +0000
parents	80e18c0836e5
children	33fac436dd54

rev	line source
0 462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	1 <tool id="varvamp" name="varVAMP" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="23.0">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	2 <description>design primers for highly diverse viruses</description>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	3 <macros>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	4 <import>macros.xml</import>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	5 </macros>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	6 <xrefs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	7 <xref type="bio.tools">varvamp</xref>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	8 </xrefs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	9 <requirements>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	10 <requirement type="package" version="@TOOL_VERSION@">varvamp</requirement>
1 80e18c0836e5 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 1626c59de1e3aa8a9ccb8674b38a4654a03ab44b iuc parents: 0 diff changeset	11 <requirement type="package" version="2.0.1">primer3-py</requirement>
80e18c0836e5 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 1626c59de1e3aa8a9ccb8674b38a4654a03ab44b iuc parents: 0 diff changeset	12 <requirement type="package" version="0.7.17">seqfold</requirement>
0 462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	13 </requirements>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	14 <version_command>varvamp --version</version_command>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	15 <command detect_errors="exit_code"><![CDATA[
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	16 VARVAMP_CONFIG=custom_config varvamp
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	17
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	18 $mode.m_select
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	19 #if $mode.main_params.specify_how in ("set_threshold", "set_both"):
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	20 --threshold $mode.main_params.threshold
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	21 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	22 #if $mode.main_params.specify_how in ("set_n_ambig", "set_both"):
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	23 --n-ambig $mode.main_params.n_ambig
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	24 #if $mode.m_select == "qpcr":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	25 --pn-ambig $mode.main_params.pn_ambig
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	26 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	27 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	28 #if str( $mode.m_select ) == "single":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	29 --opt-length $mode.opt_length
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	30 --max-length $mode.max_length
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	31 #if $mode.limit_report.choice == "yes":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	32 --report-n $mode.limit_report.report_n
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	33 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	34 #elif str( $mode.m_select ) == "tiled":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	35 --opt-length $mode.opt_length
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	36 --max-length $mode.max_length
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	37 --overlap $mode.overlap
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	38 #elif str( $mode.m_select ) == "qpcr":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	39 --test-n $mode.test_n
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	40 --deltaG $mode.deltaG
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	41 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	42 #if $mode.filter_blast_hits.choice == "yes":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	43 --database '${mode.filter_blast_hits.database.extra_files_path}/blastdb'
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	44 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	45 --threads \${GALAXY_SLOTS:-1}
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	46
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	47 '$alignment'
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	48 results/
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	49
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	50 #if $mode.m_select == 'qpcr' and $mode.scheme_outputs and 'primer_seqs' in $mode.scheme_outputs:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	51 ## make the primer sequences fasta discoverable under the same name that is used in "single" mode
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	52 && mv results/oligos.fasta results/primers.fasta
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	53 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	54 #if $mode.m_select == 'tiled' and $mode.scheme_outputs and 'primer_dimers' in $mode.scheme_outputs:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	55 ## ensure the unsolvable_primer_dimers.tsv file, which varVAMP creates only conditionally, exists in all cases, in which we try to discover it as an output
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	56 && cp -n dimers_fallback.tsv results/unsolvable_primer_dimers.tsv
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	57 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	58 ]]></command>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	59 <configfiles>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	60 <configfile filename="custom_config"><![CDATA[#if $mode.advanced_config.customize == "yes":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	61 PRIMER_TMP = ($mode.advanced_config.basic_primer_params.PRIMER_TMP_min, $mode.advanced_config.basic_primer_params.PRIMER_TMP_max, $mode.advanced_config.basic_primer_params.PRIMER_TMP_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	62 PRIMER_GC_RANGE = ($mode.advanced_config.basic_primer_params.PRIMER_GC_RANGE_min, $mode.advanced_config.basic_primer_params.PRIMER_GC_RANGE_max, $mode.advanced_config.basic_primer_params.PRIMER_GC_RANGE_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	63 PRIMER_SIZES = ($mode.advanced_config.basic_primer_params.PRIMER_SIZES_min, $mode.advanced_config.basic_primer_params.PRIMER_SIZES_max, $mode.advanced_config.basic_primer_params.PRIMER_SIZES_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	64 PRIMER_MAX_POLYX = $mode.advanced_config.basic_primer_params.PRIMER_MAX_POLYX
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	65 PRIMER_MAX_DINUC_REPEATS = $mode.advanced_config.basic_primer_params.PRIMER_MAX_DINUC_REPEATS
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	66 PRIMER_HAIRPIN = $mode.advanced_config.basic_primer_params.PRIMER_HAIRPIN
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	67 PRIMER_GC_END = ($mode.advanced_config.basic_primer_params.PRIMER_GC_END_min, $mode.advanced_config.basic_primer_params.PRIMER_GC_END_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	68 PRIMER_MIN_3_WITHOUT_AMB = $mode.advanced_config.basic_primer_params.PRIMER_MIN_3_WITHOUT_AMB
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	69 PRIMER_MAX_DIMER_TMP = $mode.advanced_config.basic_primer_params.PRIMER_MAX_DIMER_TMP
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	70 #if str($mode.m_select) == "qpcr":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	71 QPROBE_TMP = ($mode.advanced_config.qpcr_params.QPROBE_TMP_min, $mode.advanced_config.qpcr_params.QPROBE_TMP_max, $mode.advanced_config.qpcr_params.QPROBE_TMP_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	72 QPROBE_SIZES = ($mode.advanced_config.qpcr_params.QPROBE_SIZES_min, $mode.advanced_config.qpcr_params.QPROBE_SIZES_max, $mode.advanced_config.qpcr_params.QPROBE_SIZES_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	73 QPROBE_GC_RANGE = ($mode.advanced_config.qpcr_params.QPROBE_GC_RANGE_min, $mode.advanced_config.qpcr_params.QPROBE_GC_RANGE_max, $mode.advanced_config.qpcr_params.QPROBE_GC_RANGE_opt)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	74 QPROBE_GC_END = ($mode.advanced_config.qpcr_params.QPROBE_GC_END_min, $mode.advanced_config.qpcr_params.QPROBE_GC_END_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	75 QPRIMER_DIFF = $mode.advanced_config.qpcr_params.QPRIMER_DIFF
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	76 QPROBE_TEMP_DIFF = ($mode.advanced_config.qpcr_params.QPROBE_TEMP_DIFF_min, $mode.advanced_config.qpcr_params.QPROBE_TEMP_DIFF_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	77 QPROBE_DISTANCE = ($mode.advanced_config.qpcr_params.QPROBE_DISTANCE_min, $mode.advanced_config.qpcr_params.QPROBE_DISTANCE_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	78 END_OVERLAP = $mode.advanced_config.qpcr_params.END_OVERLAP
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	79 QAMPLICON_LENGTH = ($mode.advanced_config.qpcr_params.QAMPLICON_LENGTH_min, $mode.advanced_config.qpcr_params.QAMPLICON_LENGTH_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	80 QAMPLICON_GC = ($mode.advanced_config.qpcr_params.QAMPLICON_GC_min, $mode.advanced_config.qpcr_params.QAMPLICON_GC_max)
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	81 QAMPLICON_DEL_CUTOFF = $mode.advanced_config.qpcr_params.QAMPLICON_DEL_CUTOFF
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	82 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	83 PCR_MV_CONC = $mode.advanced_config.pcr_params.PCR_MV_CONC
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	84 PCR_DV_CONC = $mode.advanced_config.pcr_params.PCR_DV_CONC
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	85 PCR_DNTP_CONC = $mode.advanced_config.pcr_params.PCR_DNTP_CONC
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	86 PCR_DNA_CONC = $mode.advanced_config.pcr_params.PCR_DNA_CONC
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	87 PRIMER_TM_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_TM_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	88 PRIMER_GC_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_GC_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	89 PRIMER_SIZE_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_SIZE_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	90 PRIMER_MAX_BASE_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_MAX_BASE_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	91 PRIMER_3_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_3_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	92 PRIMER_PERMUTATION_PENALTY = $mode.advanced_config.penalty_multipliers.PRIMER_PERMUTATION_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	93 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	94 #if $mode.filter_blast_hits.choice == "yes":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	95 #if $mode.filter_blast_hits.blast_advanced_cond.blast_advanced == "yes":
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	96 BLAST_SETTINGS = {
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	97 "outfmt": "6 qseqid sseqid qlen length mismatch gapopen sstart send sstrand",
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	98 "evalue": $mode.filter_blast_hits.blast_advanced_cond.blast_params.evalue,
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	99 "reward": $mode.filter_blast_hits.blast_advanced_cond.blast_params.reward,
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	100 "penalty": $mode.filter_blast_hits.blast_advanced_cond.blast_params.penalty,
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	101 "gapopen": $mode.filter_blast_hits.blast_advanced_cond.blast_params.gapopen,
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	102 "gapextend": $mode.filter_blast_hits.blast_advanced_cond.blast_params.gapextend
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	103 }
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	104 BLAST_MAX_DIFF = $mode.filter_blast_hits.blast_advanced_cond.blast_hits_usage.BLAST_MAX_DIFF
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	105 BLAST_SIZE_MULTI = $mode.filter_blast_hits.blast_advanced_cond.blast_hits_usage.BLAST_SIZE_MULTI
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	106 BLAST_PENALTY = $mode.filter_blast_hits.blast_advanced_cond.blast_hits_usage.BLAST_PENALTY
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	107 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	108 #end if
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	109 ]]></configfile>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	110 <configfile filename="dimers_fallback.tsv"><![CDATA[#set $line = '\t'.join(['pool', 'primer_name_1', 'primer_name_2', 'dimer melting temp'])
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	111 $line]]></configfile>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	112 </configfiles>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	113 <inputs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	114 <param name="alignment" type="data" format="fasta" label="Multiple alignment of viral sequences" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	115 <conditional name="mode">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	116 <param name="m_select" type="select" label="What kind of primers would you like to design? (varvamp mode)">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	117 <option value="single">Primers for single amplicons (single)</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	118 <option value="tiled">Tiled primer scheme for whole-genome sequencing (tiled)</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	119 <option value="qpcr">qPCR primers (qpcr)</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	120 </param>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	121 <when value="single">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	122 <expand macro="main_parameters" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	123 <expand macro="amplicon_length_restrictions" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	124 <expand macro="blast_options" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	125 <conditional name="limit_report">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	126 <param name="choice" type="select" label="Limit the number of amplicons to report?">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	127 <option value="no">No, report all qualifying amplicons</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	128 <option value="yes">Yes, set an upper limit on amplicons to be reported</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	129 </param>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	130 <when value="no" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	131 <when value="yes">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	132 <param argument="--report-n" type="integer" min="1" value="20" label="Report only the n highest scoring amplicons" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	133 </when>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	134 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	135 <expand macro="customize_advanced" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	136 <expand macro="primer_scheme_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	137 <option value="amplicon_assignment">Primer-to-amplicon assignment in tabular format; lists primers belonging to the same amplicon on one line for simpler automated parsing</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	138 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	139 <expand macro="consensus_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	140 <expand macro="graphical_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	141 <expand macro="miscellaneous_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	142 <option value="all_primers">Binding sites of ALL high-scoring primers that were considered in BED format; includes primer penalties (lower is better) as the score column</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	143 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	144 </when>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	145 <when value="tiled">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	146 <expand macro="main_parameters" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	147 <expand macro="amplicon_length_restrictions" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	148 <param argument="--overlap" type="integer" min="1" value="100" label="Minimal required overlap between tiled amplicons" help="default: 100" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	149 <expand macro="blast_options" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	150 <expand macro="customize_advanced" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	151 <expand macro="primer_scheme_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	152 <option value="amplicon_assignment" selected="true">Primer-to-amplicon assignment in tabular format; lists primers belonging to the same amplicon on one line; required input for automated primer trimming in some downstream analysis workflows</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	153 <option value="primer_dimers" selected="true">If any primers in the tiling scheme are predicted to form primer dimers, details about these will be found in this tabular output.</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	154 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	155 <expand macro="consensus_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	156 <expand macro="graphical_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	157 <expand macro="miscellaneous_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	158 <option value="all_primers">Binding sites of ALL high-scoring primers that were considered in BED format; includes primer penalties (lower is better) as the score column</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	159 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	160 </when>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	161 <when value="qpcr">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	162 <expand macro="main_parameters">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	163 <param argument="--pn-ambig" type="integer" min="0" value="1" label="Maximum number of ambiguous nucleotides per qPCR probe to be tolerated" help="To enforce specificity of detection, varVAMP will refuse to work if you set this value higher than for the amplicon primers above, and you may actually want to set it slightly lower than that value." />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	164 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	165 <param argument="--test-n" type="integer" min="1" value="50" label="Top n qPCR amplicons to test" help="test the top n qPCR amplicons for secondary structures at the minimal primer temperature. (default: 50)" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	166 <param argument="--deltaG" type="integer" value="-3" label="Minimum free energy (kcal/mol/K) cutoff" help="Minimum free energy (kcal/mol/K) cutoff at the lowest primer melting temperature. (default: -3." />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	167 <expand macro="blast_options" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	168 <expand macro="customize_advanced">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	169 <section name="qpcr_params" title="Constraints on qPCR probes and amplicons" expanded="false">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	170 <!--basic probe parameters-->
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	171 <param name="QPROBE_TMP_min" type="integer" min="0" value="64" label="Minimal qPCR probe melting temperature" help="With varVAMP default settings, the probe melting temperatures are 7°C higher than those of amplicon primers." />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	172 <param name="QPROBE_TMP_max" type="integer" min="0" value="70" label="Maximal qPCR probe melting temperature" help="With varVAMP default settings, the probe melting temperatures are 7°C higher than those of amplicon primers." />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	173 <param name="QPROBE_TMP_opt" type="integer" min="0" value="67" label="Optimal qPCR probe melting temperature" help="With varVAMP default settings, the probe melting temperatures are 7°C higher than those of amplicon primers." />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	174 <param name="QPROBE_SIZES_min" type="integer" min="0" value="20" label="Minimal qPCR probe size" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	175 <param name="QPROBE_SIZES_max" type="integer" min="0" value="30" label="Maximal qPCR probe size" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	176 <param name="QPROBE_SIZES_opt" type="integer" min="0" value="25" label="Optimal qPCR probe size" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	177 <param name="QPROBE_GC_RANGE_min" type="integer" min="0" max="100" value="40" label="Minimal qPCR probe GC-content" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	178 <param name="QPROBE_GC_RANGE_max" type="integer" min="0" max="100" value="80" label="Maximal qPCR probe GC-content" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	179 <param name="QPROBE_GC_RANGE_opt" type="integer" min="0" max="100" value="60" label="Optimal qPCR probe GC-content" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	180 <param name="QPROBE_GC_END_min" type="integer" min="0" max="5" value="0" label="Minimal number of GCs among the 3'-terminal 5 bases of the probe" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	181 <param name="QPROBE_GC_END_max" type="integer" min="0" max="5" value="4" label="Maximal number of GCs among the 3'-terminal 5 bases of the probe" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	182 <!--constraints for amplicon design-->
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	183 <param name="QPRIMER_DIFF" type="integer" min="0" max="10" value="2" label="Maximal melting temperature difference among qPCR primers" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	184 <param name="QPROBE_TEMP_DIFF_min" type="integer" min="0" max="20" value="5" label="Minimal melting temperature difference between qPCR probe and primers" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	185 <param name="QPROBE_TEMP_DIFF_max" type="integer" min="0" max="20" value="10" label="Maximal melting temperature difference between qPCR probe and primers" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	186 <param name="QPROBE_DISTANCE_min" type="integer" min="0" max="20" value="4" label="Minimal distance of the qPCR probe from the primer on the same strand" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	187 <param name="QPROBE_DISTANCE_max" type="integer" min="0" max="20" value="15" label="Maximal distance of the qPCR probe from the primer on the same strand" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	188 <param name="END_OVERLAP" type="integer" min="0" max="10" value="5" label="End Overlap" help="Maximal overlap in bases between the ends of the qPCR probe and the primer on the opposite strand" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	189 <param name="QAMPLICON_LENGTH_min" type="integer" min="0" max="300" value="70" label="Minimal length of qPCR amplicons" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	190 <param name="QAMPLICON_LENGTH_max" type="integer" min="0" max="300" value="200" label="Maximal length of qPCR amplicons" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	191 <param name="QAMPLICON_GC_min" type="integer" min="0" max="100" value="40" label="Minimal GC-content of qPCR amplicons" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	192 <param name="QAMPLICON_GC_max" type="integer" min="0" max="100" value="60" label="Maximal GC-content of qPCR amplicons" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	193 <param name="QAMPLICON_DEL_CUTOFF" type="integer" min="0" max="10" value="4" label="Cutoff for deletions allowed in aligned regions under qPCR amplicons" help ="qPCR amplicon candidates are not considered further if they span regions of the alignment that contain deletions as long as this cutoff in bases, or longer."/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	194 </section>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	195 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	196 <expand macro="primer_scheme_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	197 <option value="qpcr_design" selected="true">qPCR amplicon details in tabular format</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	198 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	199 <expand macro="consensus_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	200 <expand macro="graphical_outputs" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	201 <expand macro="miscellaneous_outputs">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	202 <option value="probe_regions">List of all candidate regions of the consensus sequence that were evaluated for qPCR probes in BED format</option>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	203 </expand>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	204 </when>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	205 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	206 </inputs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	207 <outputs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	208 <data name="varvamp_log" format="txt" from_work_dir="results/varvamp_log.txt" label="${tool.name} on ${on_string}: Analysis Log" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	209 <data name="primer_seqs" format="fasta" from_work_dir="results/primers.fasta" label="${tool.name} on ${on_string}: Sequences of designed oligos">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	210 <filter>mode['m_select'] in ('single', 'qpcr') and mode['scheme_outputs'] and 'primer_seqs' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	211 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	212 <collection name="primer_seqs_collection" type="list" label="${tool.name} on ${on_string}: per-pool primer sequences">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	213 <filter>mode['m_select'] == 'tiled' and mode['scheme_outputs'] and 'primer_seqs' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	214 <data name="pool1_sequences" format="fasta" from_work_dir="results/primers_pool_0.fasta" label="${tool.name} on ${on_string}: Sequences of designed pool 1 primers" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	215 <data name="pool2_sequences" format="fasta" from_work_dir="results/primers_pool_1.fasta" label="${tool.name} on ${on_string}: Sequences of designed pool 2 primers" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	216 </collection>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	217 <data name="primers_bed" format="bed" from_work_dir="results/primers.bed" label="${tool.name} on ${on_string}: Primer binding sites">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	218 <filter>mode['scheme_outputs'] and 'primer_binding_sites' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	219 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	220 <data name="amplicons_bed" format="bed" from_work_dir="results/amplicons.bed" label="${tool.name} on ${on_string}: Amplicon locations">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	221 <filter>mode['scheme_outputs'] and 'amplicon_sites' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	222 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	223 <data name="primers_details" format="tabular" from_work_dir="results/*primers.tsv" label="${tool.name} on ${on_string}: Primer details">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	224 <filter>mode['scheme_outputs'] and 'primer_details' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	225 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	226 <data name="qpcr_details" format="tabular" from_work_dir="results/qpcr_design.tsv" label="${tool.name} on ${on_string}: qPCR amplicon details" >
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	227 <filter>mode['scheme_outputs'] and 'qpcr_design' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	228 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	229 <data name="primer_amplicon_assignments" format="tabular" from_work_dir="results/primer_to_amplicon_assignment.tabular" label="${tool.name} on ${on_string}: Primer to amplicon assignments">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	230 <filter>mode['scheme_outputs'] and 'amplicon_assignment' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	231 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	232 <data name="unresolved_primer_dimers" format="tabular" from_work_dir="results/unsolvable_primer_dimers.tsv" label="${tool.name} on ${on_string}: Unresolved primer dimers">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	233 <filter>mode['scheme_outputs'] and 'primer_dimers' in mode['scheme_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	234 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	235 <data name="ambiguous_consensus" format="fasta" from_work_dir="results/ambiguous_consensus.fasta" label="${tool.name} on ${on_string}: Ambiguous consensus sequence">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	236 <filter>mode['aln_cons_outputs'] and 'ambiguous_consensus' in mode['aln_cons_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	237 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	238 <data name="majority_consensus" format="fasta" from_work_dir="results/data/majority_consensus.fasta" label="${tool.name} on ${on_string}: Majority consensus sequence (no ambiguity codes)">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	239 <filter>mode['aln_cons_outputs'] and 'majority_consensus' in mode['aln_cons_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	240 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	241 <data name="alignment_cleaned" format="fasta" from_work_dir="results/data/alignment_cleaned.fasta" label="${tool.name} on ${on_string}: Alignment cleaned">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	242 <filter>mode['aln_cons_outputs'] and 'alignment_cleaned' in mode['aln_cons_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	243 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	244 <data name="amplicon_plot" format="pdf" from_work_dir="results/amplicon_plot.pdf" label="${tool.name} on ${on_string}: Amplicon design overview plot">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	245 <filter>mode['plot_outputs'] and 'amplicon_plot' in mode['plot_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	246 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	247 <data name="per_base_mismatches" format="pdf" from_work_dir="results/per_base_mismatches.pdf" label="${tool.name} on ${on_string}: Per-base mismatches barplots">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	248 <filter>mode['plot_outputs'] and 'per_base_mismatches' in mode['plot_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	249 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	250 <data name="primer_regions" format="bed" from_work_dir="results/data/primer_regions.bed" label="${tool.name} on ${on_string}: All candidate primer regions">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	251 <filter>mode['misc_outputs'] and 'primer_regions' in mode['misc_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	252 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	253 <data name="probe_regions" format="bed" from_work_dir="results/data/probe_regions.bed" label="${tool.name} on ${on_string}: All candidate qPCR probe regions">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	254 <filter>mode['misc_outputs'] and 'probe_regions' in mode['misc_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	255 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	256 <data name="all_primers" format="bed" from_work_dir="results/data/all_primers.bed" label="${tool.name} on ${on_string}: All primer sites">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	257 <filter>mode['misc_outputs'] and 'all_primers' in mode['misc_outputs']</filter>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	258 </data>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	259 </outputs>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	260 <tests>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	261 <test expect_num_outputs="8">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	262 <param name="alignment" value="hepatitis_e_aln_shrunk.fasta"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	263 <conditional name="mode">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	264 <param name='m_select' value="single"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	265 <conditional name="main_params">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	266 <param name="specify_how" value="set_both"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	267 <param name="threshold" value="0.8"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	268 <param name="n_ambig" value="3"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	269 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	270 <param name="opt_length" value="300"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	271 <param name="max_length" value="400"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	272 <conditional name="limit_report">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	273 <param name="choice" value="yes" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	274 <param name="report_n" value="5"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	275 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	276 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	277 <output name="varvamp_log" ftype="txt" compare="contains" file="test1.log" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	278 <output name="ambiguous_consensus" ftype="fasta" file="ambiguous_consensus.fasta" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	279 <output name="amplicon_plot" ftype="pdf">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	280 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	281 <has_text text="%PDF"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	282 <has_text text="%%EOF"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	283 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	284 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	285 <output name="primers_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	286 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	287 <has_n_lines n="2" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	288 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	289 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	290 <output name="amplicons_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	291 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	292 <has_n_lines n="1"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	293 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	294 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	295 <output name="per_base_mismatches" ftype="pdf">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	296 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	297 <has_text text="%PDF"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	298 <has_text text="%%EOF"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	299 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	300 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	301 <output name="primer_seqs" ftype="fasta">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	302 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	303 <has_line line=">AMPLICON_0_LEFT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	304 <has_line line=">AMPLICON_0_RIGHT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	305 <has_n_lines n="4"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	306 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	307 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	308 </test>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	309 <test expect_num_outputs="12">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	310 <param name="alignment" value="hepatitis_e_aln_shrunk.fasta"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	311 <conditional name="mode">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	312 <param name='m_select' value="tiled"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	313 <conditional name="main_params">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	314 <param name="specify_how" value="set_both"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	315 <param name="threshold" value="0.6"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	316 <param name="n_ambig" value="2"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	317 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	318 <param name="opt_length" value="150"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	319 <param name="max_length" value="300"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	320 <param name="overlap" value="100"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	321 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	322 <output name="varvamp_log" ftype="txt" compare="contains" file="test2.log" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	323 <output name="primers_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	324 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	325 <has_n_lines n="4" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	326 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	327 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	328 <output name="amplicons_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	329 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	330 <has_n_lines n="2"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	331 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	332 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	333 <output name="primer_amplicon_assignments" ftype="tabular">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	334 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	335 <has_n_lines n="2"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	336 <has_n_columns n="2"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	337 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	338 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	339 <output name="unresolved_primer_dimers" ftype="tabular">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	340 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	341 <has_n_lines n="1"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	342 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	343 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	344 <output_collection name="primer_seqs_collection" type="list" count="2">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	345 <element name="pool1_sequences" ftype="fasta">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	346 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	347 <has_n_lines n="4"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	348 <has_line line=">AMPLICON_0_LEFT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	349 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	350 </element>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	351 <element name="pool2_sequences" ftype="fasta">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	352 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	353 <has_n_lines n="4"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	354 <has_line line=">AMPLICON_1_LEFT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	355 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	356 </element>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	357 </output_collection>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	358 </test>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	359 <test expect_num_outputs="9">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	360 <param name="alignment" value="hepatitis_e_aln_shrunk.fasta"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	361 <conditional name="mode">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	362 <param name='m_select' value="qpcr"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	363 <conditional name="main_params">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	364 <param name="specify_how" value="set_both"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	365 <param name="threshold" value="0.7"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	366 <param name="n_ambig" value="1"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	367 <param name="pn_ambig" value="1"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	368 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	369 <param name="test_n" value="180"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	370 <param name="deltaG" value="-15"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	371 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	372 <output name="varvamp_log" ftype="txt" compare="contains" file="test3.log" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	373 <output name="primers_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	374 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	375 <has_n_lines n="3" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	376 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	377 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	378 <output name="amplicons_bed" ftype="bed">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	379 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	380 <has_n_lines n="1"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	381 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	382 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	383 <output name="primer_seqs" ftype="fasta">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	384 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	385 <has_line line=">QPCR_SCHEME_0_PROBE"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	386 <has_line line=">QPCR_SCHEME_0_LEFT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	387 <has_line line=">QPCR_SCHEME_0_RIGHT"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	388 <has_n_lines n="6"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	389 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	390 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	391 <output name="qpcr_details" ftype="tabular">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	392 <assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	393 <has_n_lines n="2"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	394 </assert_contents>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	395 </output>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	396 </test>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	397 <test expect_num_outputs="1">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	398 <!-- Similar to test 1, but try to modify some of varVAMP's
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	399 advanced params, and see whether that gets reflected in the log file.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	400 -->
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	401 <param name="alignment" value="hepatitis_e_aln_shrunk.fasta"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	402 <conditional name="mode">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	403 <param name='m_select' value="single"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	404 <conditional name="main_params">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	405 <param name="specify_how" value="set_both"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	406 <param name="threshold" value="0.8"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	407 <param name="n_ambig" value="3"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	408 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	409 <param name="opt_length" value="300"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	410 <param name="max_length" value="400"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	411 <conditional name="limit_report">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	412 <param name="choice" value="yes" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	413 <param name="report_n" value="5"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	414 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	415 <conditional name="advanced_config">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	416 <param name="customize" value="yes"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	417 <section name="basic_primer_params">
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	418 <param name="PRIMER_GC_RANGE_min" value="20"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	419 <param name="PRIMER_GC_RANGE_max" value="80"/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	420 </section>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	421 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	422 <param name="scheme_outputs" value=""/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	423 <param name="aln_cons_outputs" value=""/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	424 <param name="plot_outputs" value=""/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	425 <param name="misc_outputs" value=""/>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	426 </conditional>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	427 <output name="varvamp_log" ftype="txt" compare="contains" file="test4.log" />
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	428 </test>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	429 </tests>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	430 <help><![CDATA[
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	431
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	432 .. class:: infomark
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	433
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	434 What it does
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	435
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	436 variable VirusAMPlicons (varVAMP) is a tool to design primers for highly diverse viruses. The input is an alignment of your viral (full-genome) sequences.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	437
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	438 For a lot of virus genera it is difficult to design pan-specific primers. varVAMP solves this by introducing ambiguous characters into primers and minimizes mismatches at the 3' end. Primers might not work for some sequences of your input alignment but should recognize the large majority.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	439
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	440 varVAMP can be run in three different modes:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	441
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	442 * SINGLE: varVAMP searches for the very best primers and reports back non-overlapping amplicons which can be used for PCR-based screening approaches.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	443 * TILED: varVAMP uses a graph based approach to design overlapping (tiled) amplicons that together cover the entire viral genome. This designs amplicons that are suitable for Oxford Nanopore or Illumina based full-genome sequencing.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	444 * QPCR: varVAMP searches for small amplicons with an optimized internal probe (TaqMan). It minimizes temperature differences between the primers and checks for amplicon secondary structures.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	445
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	446 Algorithmic parameters:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	447
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	448 These are explained very well on the `How varVAMP works <https://github.com/jonas-fuchs/varVAMP/blob/master/docs/how_varvamp_works.md>`__ page of the varVAMP documentation.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	449
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	450 Outputs:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	451
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	452 This tool can produce lots of informative output and the different modes come with what we hope are reasonable default choices.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	453
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	454 The most important output, however, is the Analysis Log, which will always be generated and contains essential information about what parameters where used by varVAMP during the run and about intermediate results. You should always check this file. Together with the tool's standard output and error streams, it is the place to spot issues with your analysis and to start debugging them.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	455
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	456 .. class:: infomark
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	457
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	458 Testing with a new alignment/analysis:
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	459
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	460 As a first check whether a new alignment of viral sequences can be used with specific settings of the tool, it may help to disable all configurable outputs, which will leave you with only the Analysis Log. This will be enough to see if there are any errors with the combination of alignment and settings, and whether you are on a roughly correct track (varVAMP reports some primers, for example). This way, you're not flooding your analysis history with lots of likely useless datasets, and once you've fixed potential issues you can add back more outputs and rerun the analysis.
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	461 ]]></help>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	462 <citations>
1 80e18c0836e5 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 1626c59de1e3aa8a9ccb8674b38a4654a03ab44b iuc parents: 0 diff changeset	463 <citation type="doi">10.5281/zenodo.10908223</citation>
0 462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	464 </citations>
462e62be07e1 planemo upload for repository https://github.com/jonas-fuchs/varVAMP commit 7f509fd29e994d1561ff9de14f26cf49f018dd2d iuc parents: diff changeset	465 </tool>

Mercurial > repos > iuc > varvamp

annotate varvamp.xml @ 2:eb5dcbebd242 draft