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planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit b1be52deee49703c602af519df3515e6ae957167
author iuc
date Sun, 25 Feb 2024 18:11:24 +0000
parents fb20fa735dfe
children
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1 <?xml version="1.0"?>
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2 <tool id="vcfanno" name="vcfanno" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" profile="@PROFILE@">
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3 <description>VCF annotation</description>
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4 <macros>
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5 <import>macros.xml</import>
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6 </macros>
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7
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8 <expand macro="xrefs"/>
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9
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10 <expand macro="requirements"/>
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11
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12 <expand macro="version_command"/>
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13
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14 <!--
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15 In future versions - figure out how to safely add in lua support without posing security risk
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16 (Should be doable but requires writing some lua unfortunately to make custom sandboxes)
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17 -->
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18 <command detect_errors="exit_code"><![CDATA[
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19 ln -s '$config_toml' config.toml &&
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20 ln -s '$input_vcf' input.vcf &&
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21
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22 #for $i, $annotation in enumerate($annotations):
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23 #set $ext = str($annotation.type.type_select)
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24 ln -s '$annotation.type.annotation' 'input_${i}.${ext}' &&
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25 ### These sets of conversions etc shouldn't be necessary but the vcfanno in Galaxy looks for an index file regardless of compression for some reason and if not present fails.
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26 #if $ext == 'vcf' or $ext == 'bed':
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27 bgzip 'input_${i}.${ext}' &&
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28 #set $ext = $ext + ".gz"
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29 #end if
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30 #if '.gz' in $ext[-3:]:
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31 tabix -p vcf 'input_${i}.${ext}' &&
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32 #elif $ext == 'bam':
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33 ln -s '${annotation.type.annotation.metadata.bam_index}' input_${i}.bam.bai &&
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34 #end if
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35 #end for
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36 vcfanno -p \${GALAXY_SLOTS:-4}
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37 #if $ends:
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38 '$ends'
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39 #end if
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40 #if $permissive_overlap:
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41 '$permissive_overlap'
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42 #end if
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43 config.toml
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44 input.vcf
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45 >
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46 '$out_vcf'
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47 ]]></command>
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48 <configfiles>
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49 <configfile name="config_toml">#
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50 #for $i, $annotation in enumerate($annotations):
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51 #set $ext = $annotation.type.type_select
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52 #if $ext == 'vcf' or $ext == 'bed':
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53 #set $ext = $ext + ".gz"
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54 #end if
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55 #if $ext == 'vcf.gz' or $ext == 'bam':
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56 #set $annotation_names = list()
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57 #set $annotation_fields = list()
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58 #set $annotation_ops = list()
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59 #for $field in $annotation.type.fields:
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60 #silent $annotation_names.append('"%s"' %(str($field.name)))
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61 #silent $annotation_fields.append('"%s"' %(str($field.field)))
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62 #silent $annotation_ops.append('"%s"' %(str($field.op)))
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63 #end for
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64 #set $names = "names= [%s]" % (", ".join($annotation_names))
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65 #set $fields = "fields= [%s]" % (", ".join($annotation_fields))
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66 #set $ops = "ops= [%s]" % (", ".join($annotation_ops))
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67 #elif $ext == 'bed.gz':
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68 #set $annotation_names = list()
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69 #set $annotation_columns = list()
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70 #set $annotation_ops = list()
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71 #for $field in $annotation.type.fields:
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72 #silent $annotation_names.append('"%s"' %(str($field.name)))
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73 #silent $annotation_columns.append( str($field.columns))
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74 #silent $annotation_ops.append('"%s"' %(str($field.op)))
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75 #end for
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76 #set $names = "names= [%s]" % (", ".join($annotation_names))
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77 #set $fields = "columns= [%s]" % (", ".join($annotation_columns))
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78 #set $ops = "ops= [%s]" % (", ".join($annotation_ops))
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79 #end if
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80 [[annotation]]
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81 file="input_${i}.${ext}"
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82 ${names}
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83 ${fields}
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84 ${ops}
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85
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86 #end for
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87 </configfile>
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88 </configfiles>
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89 <inputs>
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90 <param name="input_vcf" type="data" format="vcf,vcf_bgzip" label="VCF to be annotated"/>
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91 <param argument="-ends" type="boolean" checked="false" truevalue="-ends" falsevalue="" label="annotate the start and end as well as the interval itself?"/>
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92 <param argument="-permissive-overlap" type="boolean" checked="false" truevalue="-permissive-overlap" falsevalue="" label="annotate with an overlapping variant even it doesn't share the same ref and alt alleles." help="Default is to require exact match between variants."/>
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93 <repeat name="annotations" title="Input annotations" min="1">
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94 <conditional name="type">
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95 <param name="type_select" type="select">
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96 <option value="vcf" selected="true">VCF</option>
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97 <option value="vcf.gz">VCF.bgzip</option>
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98 <option value="bed">BED</option>
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99 <option value="bam">BAM</option>
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100 </param>
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101 <when value="vcf">
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102 <param name="annotation" type="data" format="vcf" label="Annotation file"/>
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103 <repeat name="fields" title="Fields" min="1">
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104 <param name="name" type="text"/>
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105 <param name="field" type="text" help="ID and FILTER are special fields that pull the ID and FILTER columns from the VCF"/>
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106 <param name="op" type="select" label="Op" help="In most cases, we will have a single annotation entry for each entry (variant) in the query VCF, in which case the self op is the best choice. However, it is possible that there will be multiple annotations from a single annotation file--in this case, the op determines how the many values are reduced. In nearly all cases, if you are annotating with a VCF, use self">
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107 <expand macro="ops"/>
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108 </param>
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109 </repeat>
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110 </when>
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111 <when value="vcf.gz">
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112 <param name="annotation" type="data" format="vcf_bgzip" label="Annotation file"/>
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113 <repeat name="fields" title="Fields" min="1">
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114 <param name="name" type="text"/>
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115 <param name="field" type="text" help="ID and FILTER are special fields that pull the ID and FILTER columns from the VCF"/>
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116 <param name="op" type="select" label="Op" help="In most cases, we will have a single annotation entry for each entry (variant) in the query VCF, in which case the self op is the best choice. However, it is possible that there will be multiple annotations from a single annotation file--in this case, the op determines how the many values are reduced. In nearly all cases, if you are annotating with a VCF, use self">
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117 <expand macro="ops"/>
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118 </param>
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119 </repeat>
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120 </when>
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121 <when value="bed">
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122 <param name="annotation" type="data" format="bed" label="Annotation file"/>
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123 <repeat name="fields" title="Fields" min="1">
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124 <param name="name" type="text"/>
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125 <param name="columns" type="text" help="(1-based) column number from which to pull the value for the op"/>
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126 <param name="op" type="select" label="Op">
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127 <expand macro="ops"/>
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128 </param>
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129 </repeat>
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130 </when>
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131 <when value="bam">
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132 <param name="annotation" type="data" format="bam" label="Annotation file"/>
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133 <repeat name="fields" title="Fields" min="1">
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134 <param name="name" type="text"/>
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135 <param name="field" type="select" help="Field in the BAM to pull value from">
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136 <option value="depth">Depth (count)</option>
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137 <option value="coverage">Coverage</option>
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138 <option value="mapq">mapq (mapping quality)</option>
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139 <option value="seq">seq</option>
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140 <option value="DP2">DP2</option>
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141 </param>
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142 <param name="op" type="select" label="Op" help="Op to perform on the field. Note: DP2 field always will result in DP2 op">
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143 <expand macro="ops"/>
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144 </param>
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145 </repeat>
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146 </when>
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147 </conditional>
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148 </repeat>
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149 <!-- <repeat name="postannotations" title="Input postannotations">
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150 </repeat> -->
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151 <!-- Until we figure out lua sanitization there's really no point to adding this either-->
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152 </inputs>
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153 <outputs>
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154 <!-- standard -->
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155 <data name="out_vcf" format="vcf" label="${tool.name} on ${on_string}: Result"/>
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156 </outputs>
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157 <tests>
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158 <!-- #1 default -->
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159 <test expect_num_outputs="1">
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160 <param name="input_vcf" value="query.vcf.gz" ftype="vcf_bgzip"/>
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161 <repeat name="annotations">
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162 <conditional name="type">
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163 <param name="type_select" value="vcf.gz" />
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164 <param name="annotation" value="exac.vcf.gz" ftype="vcf_bgzip"/>
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165 <repeat name="fields">
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166 <param name="name" value="AC_AFR" />
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167 <param name="field" value="AC_AFR" />
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168 <param name="op" value="first" />
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169 </repeat>
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170 <repeat name="fields">
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171 <param name="name" value="AC_AMR" />
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172 <param name="field" value="AC_AMR" />
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173 <param name="op" value="first" />
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174 </repeat>
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175 <repeat name="fields">
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176 <param name="name" value="AC_EAS" />
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177 <param name="field" value="AC_EAS" />
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178 <param name="op" value="first" />
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179 </repeat>
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180 <repeat name="fields">
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181 <param name="name" value="ID" />
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182 <param name="field" value="ID" />
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183 <param name="op" value="first" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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184 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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185 </conditional>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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186 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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187 <repeat name="annotations">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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188 <conditional name="type">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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189 <param name="type_select" value="bam" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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190 <param name="annotation" value="ex.bam" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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191 <repeat name="fields">
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192 <param name="name" value="mapq" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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193 <param name="field" value="mapq" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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194 <param name="op" value="mean" />
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195 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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196 <repeat name="fields">
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197 <param name="name" value="coverage" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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198 <param name="field" value="coverage" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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199 <param name="op" value="sum" />
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200 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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201 <repeat name="fields">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
202 <param name="name" value="xdp2" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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203 <param name="field" value="DP2" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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204 <param name="op" value="self" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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205 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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206 </conditional>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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207 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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208 <repeat name="annotations">
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209 <conditional name="type">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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210 <param name="type_select" value="bed" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
211 <param name="annotation" value="fitcons.bed" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
212 <repeat name="fields">
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diff changeset
213 <param name="name" value="lua_start" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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214 <param name="columns" value="4" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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215 <param name="op" value="first" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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216 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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217 <repeat name="fields">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
218 <param name="name" value="lua_end" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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219 <param name="columns" value="4" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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220 <param name="op" value="first" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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221 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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222 <repeat name="fields">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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223 <param name="name" value="lua_len" />
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224 <param name="columns" value="4" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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225 <param name="op" value="first" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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226 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
227 <repeat name="fields">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
228 <param name="name" value="lua_mean" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
229 <param name="columns" value="4" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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230 <param name="op" value="first" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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231 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
232 <repeat name="fields">
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
233 <param name="name" value="lua_loc" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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234 <param name="columns" value="4" />
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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235 <param name="op" value="first" />
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236 </repeat>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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237 </conditional>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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238 </repeat>
2
4f3c5e634679 planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit b1be52deee49703c602af519df3515e6ae957167
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239 <output name="out_vcf" file="vcfanno_result.vcf" lines_diff="4"/>
0
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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240 </test>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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241 </tests>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
242 <help><![CDATA[
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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243 .. class:: infomark
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244
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
245 **What it does**
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246
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
iuc
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diff changeset
247 @WID@
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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248
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
249 Note this version of vcfanno does not support lua code functionality
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250
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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251 **Input**
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252
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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253 - VCF file to be annotated
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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254
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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255 - BAM / BED / VCF files to annotate with
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256
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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257 **Output**
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258
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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259 - Annotated VCF
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260
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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261 **References**
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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262
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
263 @REFERENCES@
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
264 ]]></help>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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diff changeset
265 <expand macro="citations"/>
fb20fa735dfe "planemo upload for repository https://github.com/galaxyproject/tools-iuc/vcfanno/ commit d962b44c5d3fecd1a315d6fbda8831d6de1b2801"
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266 </tool>