diff vsnp_determine_ref_from_data.py @ 2:4cc004985e27 draft

"planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/master/tools/vsnp commit 524a39e08f2bea8b8754284df606ff8dd27ed24b"
author iuc
date Wed, 02 Dec 2020 09:10:53 +0000
parents
children 2e863710a2f0
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--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/vsnp_determine_ref_from_data.py	Wed Dec 02 09:10:53 2020 +0000
@@ -0,0 +1,231 @@
+#!/usr/bin/env python
+
+import argparse
+import gzip
+import os
+from collections import OrderedDict
+
+import yaml
+from Bio.SeqIO.QualityIO import FastqGeneralIterator
+
+OUTPUT_DBKEY_DIR = 'output_dbkey'
+OUTPUT_METRICS_DIR = 'output_metrics'
+
+
+def get_base_file_name(file_path):
+    base_file_name = os.path.basename(file_path)
+    if base_file_name.find(".") > 0:
+        # Eliminate the extension.
+        return os.path.splitext(base_file_name)[0]
+    elif base_file_name.find("_fq") > 0:
+        # The "." character has likely
+        # changed to an "_" character.
+        return base_file_name.split("_fq")[0]
+    elif base_file_name.find("_fastq") > 0:
+        return base_file_name.split("_fastq")[0]
+    return base_file_name
+
+
+def get_dbkey(dnaprints_dict, key, s):
+    # dnaprints_dict looks something like this:
+    # {'brucella': {'NC_002945v4': ['11001110', '11011110', '11001100']}
+    # {'bovis': {'NC_006895': ['11111110', '00010010', '01111011']}}
+    d = dnaprints_dict.get(key, {})
+    for data_table_value, v_list in d.items():
+        if s in v_list:
+            return data_table_value
+    return ""
+
+
+def get_dnaprints_dict(dnaprint_fields):
+    # A dndprint_fields entry looks something liek this.
+    # [['AF2122', '/galaxy/tool-data/vsnp/AF2122/dnaprints/NC_002945v4.yml']]
+    dnaprints_dict = {}
+    for item in dnaprint_fields:
+        # Here item is a 2-element list of data
+        # table components, # value and path.
+        value = item[0]
+        path = item[1].strip()
+        with open(path, "rt") as fh:
+            # The format of all dnaprints yaml
+            # files is something like this:
+            # brucella:
+            #  - 0111111111111111
+            print_dict = yaml.load(fh, Loader=yaml.Loader)
+        for print_dict_k, print_dict_v in print_dict.items():
+            dnaprints_v_dict = dnaprints_dict.get(print_dict_k, {})
+            if len(dnaprints_v_dict) > 0:
+                # dnaprints_dict already contains k (e.g., 'brucella',
+                # and dnaprints_v_dict will be a dictionary # that
+                # looks something like this:
+                # {'NC_002945v4': ['11001110', '11011110', '11001100']}
+                value_list = dnaprints_v_dict.get(value, [])
+                value_list = value_list + print_dict_v
+                dnaprints_v_dict[value] = value_list
+            else:
+                # dnaprints_v_dict is an empty dictionary.
+                dnaprints_v_dict[value] = print_dict_v
+            dnaprints_dict[print_dict_k] = dnaprints_v_dict
+    # dnaprints_dict looks something like this:
+    # {'brucella': {'NC_002945v4': ['11001110', '11011110', '11001100']}
+    # {'bovis': {'NC_006895': ['11111110', '00010010', '01111011']}}
+    return dnaprints_dict
+
+
+def get_group_and_dbkey(dnaprints_dict, brucella_string, brucella_sum, bovis_string, bovis_sum, para_string, para_sum):
+    if brucella_sum > 3:
+        group = "Brucella"
+        dbkey = get_dbkey(dnaprints_dict, "brucella", brucella_string)
+    elif bovis_sum > 3:
+        group = "TB"
+        dbkey = get_dbkey(dnaprints_dict, "bovis", bovis_string)
+    elif para_sum >= 1:
+        group = "paraTB"
+        dbkey = get_dbkey(dnaprints_dict, "para", para_string)
+    else:
+        group = ""
+        dbkey = ""
+    return group, dbkey
+
+
+def get_oligo_dict():
+    oligo_dict = {}
+    oligo_dict["01_ab1"] = "AATTGTCGGATAGCCTGGCGATAACGACGC"
+    oligo_dict["02_ab3"] = "CACACGCGGGCCGGAACTGCCGCAAATGAC"
+    oligo_dict["03_ab5"] = "GCTGAAGCGGCAGACCGGCAGAACGAATAT"
+    oligo_dict["04_mel"] = "TGTCGCGCGTCAAGCGGCGTGAAATCTCTG"
+    oligo_dict["05_suis1"] = "TGCGTTGCCGTGAAGCTTAATTCGGCTGAT"
+    oligo_dict["06_suis2"] = "GGCAATCATGCGCAGGGCTTTGCATTCGTC"
+    oligo_dict["07_suis3"] = "CAAGGCAGATGCACATAATCCGGCGACCCG"
+    oligo_dict["08_ceti1"] = "GTGAATATAGGGTGAATTGATCTTCAGCCG"
+    oligo_dict["09_ceti2"] = "TTACAAGCAGGCCTATGAGCGCGGCGTGAA"
+    oligo_dict["10_canis4"] = "CTGCTACATAAAGCACCCGGCGACCGAGTT"
+    oligo_dict["11_canis"] = "ATCGTTTTGCGGCATATCGCTGACCACAGC"
+    oligo_dict["12_ovis"] = "CACTCAATCTTCTCTACGGGCGTGGTATCC"
+    oligo_dict["13_ether2"] = "CGAAATCGTGGTGAAGGACGGGACCGAACC"
+    oligo_dict["14_63B1"] = "CCTGTTTAAAAGAATCGTCGGAACCGCTCT"
+    oligo_dict["15_16M0"] = "TCCCGCCGCCATGCCGCCGAAAGTCGCCGT"
+    oligo_dict["16_mel1b"] = "TCTGTCCAAACCCCGTGACCGAACAATAGA"
+    oligo_dict["17_tb157"] = "CTCTTCGTATACCGTTCCGTCGTCACCATGGTCCT"
+    oligo_dict["18_tb7"] = "TCACGCAGCCAACGATATTCGTGTACCGCGACGGT"
+    oligo_dict["19_tbbov"] = "CTGGGCGACCCGGCCGACCTGCACACCGCGCATCA"
+    oligo_dict["20_tb5"] = "CCGTGGTGGCGTATCGGGCCCCTGGATCGCGCCCT"
+    oligo_dict["21_tb2"] = "ATGTCTGCGTAAAGAAGTTCCATGTCCGGGAAGTA"
+    oligo_dict["22_tb3"] = "GAAGACCTTGATGCCGATCTGGGTGTCGATCTTGA"
+    oligo_dict["23_tb4"] = "CGGTGTTGAAGGGTCCCCCGTTCCAGAAGCCGGTG"
+    oligo_dict["24_tb6"] = "ACGGTGATTCGGGTGGTCGACACCGATGGTTCAGA"
+    oligo_dict["25_para"] = "CCTTTCTTGAAGGGTGTTCG"
+    oligo_dict["26_para_sheep"] = "CGTGGTGGCGACGGCGGCGGGCCTGTCTAT"
+    oligo_dict["27_para_cattle"] = "TCTCCTCGGTCGGTGATTCGGGGGCGCGGT"
+    return oligo_dict
+
+
+def get_seq_counts(value, fastq_list, gzipped):
+    count = 0
+    for fastq_file in fastq_list:
+        if gzipped:
+            with gzip.open(fastq_file, 'rt') as fh:
+                for title, seq, qual in FastqGeneralIterator(fh):
+                    count += seq.count(value)
+        else:
+            with open(fastq_file, 'r') as fh:
+                for title, seq, qual in FastqGeneralIterator(fh):
+                    count += seq.count(value)
+    return(value, count)
+
+
+def get_species_counts(fastq_list, gzipped):
+    count_summary = {}
+    oligo_dict = get_oligo_dict()
+    for v1 in oligo_dict.values():
+        returned_value, count = get_seq_counts(v1, fastq_list, gzipped)
+        for key, v2 in oligo_dict.items():
+            if returned_value == v2:
+                count_summary.update({key: count})
+    count_list = []
+    for v in count_summary.values():
+        count_list.append(v)
+    brucella_sum = sum(count_list[:16])
+    bovis_sum = sum(count_list[16:24])
+    para_sum = sum(count_list[24:])
+    return count_summary, count_list, brucella_sum, bovis_sum, para_sum
+
+
+def get_species_strings(count_summary):
+    binary_dictionary = {}
+    for k, v in count_summary.items():
+        if v > 1:
+            binary_dictionary.update({k: 1})
+        else:
+            binary_dictionary.update({k: 0})
+    binary_dictionary = OrderedDict(sorted(binary_dictionary.items()))
+    binary_list = []
+    for v in binary_dictionary.values():
+        binary_list.append(v)
+    brucella_binary = binary_list[:16]
+    brucella_string = ''.join(str(e) for e in brucella_binary)
+    bovis_binary = binary_list[16:24]
+    bovis_string = ''.join(str(e) for e in bovis_binary)
+    para_binary = binary_list[24:]
+    para_string = ''.join(str(e) for e in para_binary)
+    return brucella_string, bovis_string, para_string
+
+
+def output_dbkey(file_name, dbkey, output_file):
+    # Output the dbkey.
+    with open(output_file, "w") as fh:
+        fh.write("%s" % dbkey)
+
+
+def output_files(fastq_file, count_list, group, dbkey, dbkey_file, metrics_file):
+    base_file_name = get_base_file_name(fastq_file)
+    output_dbkey(base_file_name, dbkey, dbkey_file)
+    output_metrics(base_file_name, count_list, group, dbkey, metrics_file)
+
+
+def output_metrics(file_name, count_list, group, dbkey, output_file):
+    # Output the metrics.
+    with open(output_file, "w") as fh:
+        fh.write("Sample: %s\n" % file_name)
+        fh.write("Brucella counts: ")
+        for i in count_list[:16]:
+            fh.write("%d," % i)
+        fh.write("\nTB counts: ")
+        for i in count_list[16:24]:
+            fh.write("%d," % i)
+        fh.write("\nPara counts: ")
+        for i in count_list[24:]:
+            fh.write("%d," % i)
+        fh.write("\nGroup: %s" % group)
+        fh.write("\ndbkey: %s\n" % dbkey)
+
+
+if __name__ == '__main__':
+    parser = argparse.ArgumentParser()
+
+    parser.add_argument('--dnaprint_fields', action='append', dest='dnaprint_fields', nargs=2, required=False, default=None, help="List of dnaprints data table value, name and path fields")
+    parser.add_argument('--read1', action='store', dest='read1', required=True, default=None, help='Required: single read')
+    parser.add_argument('--read2', action='store', dest='read2', required=False, default=None, help='Optional: paired read')
+    parser.add_argument('--gzipped', action='store_true', dest='gzipped', default=False, help='Input files are gzipped')
+    parser.add_argument('--output_dbkey', action='store', dest='output_dbkey', required=True, default=None, help='Output reference file')
+    parser.add_argument('--output_metrics', action='store', dest='output_metrics', required=True, default=None, help='Output metrics file')
+
+    args = parser.parse_args()
+
+    fastq_list = [args.read1]
+    if args.read2 is not None:
+        fastq_list.append(args.read2)
+
+    # The value of dnaprint_fields is a list of lists, where each list is
+    # the [value, name, path] components of the vsnp_dnaprints data table.
+    # The data_manager_vsnp_dnaprints tool assigns the dbkey column from the
+    # all_fasta data table to the value column in the vsnp_dnaprints data
+    # table to ensure a proper mapping for discovering the dbkey.
+    dnaprints_dict = get_dnaprints_dict(args.dnaprint_fields)
+
+    # Here fastq_list consists of either a single read
+    # or a set of paired reads, producing single outputs.
+    count_summary, count_list, brucella_sum, bovis_sum, para_sum = get_species_counts(fastq_list, args.gzipped)
+    brucella_string, bovis_string, para_string = get_species_strings(count_summary)
+    group, dbkey = get_group_and_dbkey(dnaprints_dict, brucella_string, brucella_sum, bovis_string, bovis_sum, para_string, para_sum)
+    output_files(args.read1, count_list, group, dbkey, dbkey_file=args.output_dbkey, metrics_file=args.output_metrics)