annotate windowmasker_ustat.xml @ 0:d90ae4a02efd draft default tip

planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/windowmasker/ commit d529dafd5a2d3a017de226cd59e42cf630348e06
author iuc
date Thu, 14 Dec 2023 16:04:27 +0000
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d90ae4a02efd planemo upload for repository https://github.com/galaxyproject/tools-iuc/tree/main/tools/windowmasker/ commit d529dafd5a2d3a017de226cd59e42cf630348e06
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1 <tool id="windowmasker_ustat" name="WindowMasker ustat" version="1.0" profile="22.01">
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2 <description>Mask sequences using a WindowMasker unit counts table</description>
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3 <macros>
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4 <import>macros.xml</import>
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5 </macros>
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6 <expand macro="requirements" />
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7 <command detect_errors="exit_code">
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8 <![CDATA[
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9 windowmasker -ustat '${mkcount_input}'
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10 -infmt fasta
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11 -in '${fasta_input}'
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12 -outfmt '${output_format}'
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13 ${parse_seqids}
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14
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15 #if str($use_dust.use_dust_selector) == "yes":
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16 -dust true
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17 -dust_level ${use_dust.dust_level}
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18 #end if
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19
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20 #if $adv.set_t_high:
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21 -set_t_high $adv.set_t_high
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22 #end if
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23 #if $adv.set_t_high:
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24 -set_t_low $adv.set_t_high
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25 #end if
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26 #if $adv.t_extend
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27 -t_extend $adv.t_extend
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28 #end if
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29 #if $adv.t_low
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30 -t_low $adv.t_low
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31 #end if
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32 #if $adv.t_high
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33 -t_high $adv.t_high
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34 #end if
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35 #if $adv.t_thres
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36 -t_thres $adv.t_thres
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37 #end if
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38 #if $adv.window
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39 -window $adv.window
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40 #end if
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41
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42 ## Convert WindowMasker interval output to BED format
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43 #if str($output_format) == "interval":
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44 | perl '${__tool_directory__}/windowmasker_to_bed.pl' > '${mask_output}'
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45 #else
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46 -out '${mask_output}'
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47 #end if
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48 ]]>
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49 </command>
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50 <inputs>
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51 <param argument="-in" name="fasta_input" type="data" format="fasta"
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52 label="FASTA sequence file" />
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53
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54 <param name="mkcount_input" type="data" format="txt"
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55 label="Unit counts produced by WindowMasker mkcount" />
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56
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57 <param argument="-parse_seqids" type="boolean" checked="false"
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58 truevalue="-parse_seqids" falsevalue=""
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59 label="Parse Seq-ids in FASTA input" />
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60
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61 <conditional name="use_dust">
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62 <param argument="-dust" name="use_dust_selector" type="select" label="Use DUST to mask low complexity sequences?" >
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63 <option value="yes">Yes</option>
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64 <option value="no">No</option>
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65 </param>
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66 <when value="yes">
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67 <param argument="-dust_level" type="integer" min="1" value="20" label="DUST level"
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68 help="Score threshold for subwindows" />
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69 </when>
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70 <when value="no"></when>
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71 </conditional>
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72
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73 <param name="output_format" type="select" label="Output format">
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74 <option value="fasta">FASTA</option>
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75 <option value="interval" selected="true">BED</option>
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76 <option value="maskinfo_asn1_bin">maskinfo ASN.1 binary</option>
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77 <option value="maskinfo_asn1_text">maskinfo ASN.1 text</option>
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78 <option value="maskinfo_xml">maskinfo XML</option>
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79 </param>
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80
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81 <section name="adv" title="Advanced options" expanded="false">
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82
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83 <param argument="-set_t_high" type="integer" label="set_t_high"
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84 min="0" optional="true"
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85 help="Score for units with unit count greater than T_high" />
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86
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87 <param argument="-set_t_low" type="integer" label="set_t_low"
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88 min="0" optional="true"
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89 help="Score for units with unit count less than T_low" />
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90
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91 <param argument="-t_extend" type="integer" label="t_extend"
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92 min="0" optional="true"
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93 help="Override the t_extend value in the unit counts file" />
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94
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95 <param argument="-t_low" type="integer" label="t_low"
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96 min="0" optional="true"
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97 help="Override the t_low value in the unit counts file" />
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98
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99 <param argument="-t_high" type="integer" label="t_high"
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100 min="0" optional="true"
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101 help="Override the t_high value in the unit counts file" />
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102
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103 <param argument="-t_thres" type="integer" label="Score threshold"
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104 min="0" optional="true"
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105 help="Override the score threshold value in the unit counts file" />
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106
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107 <param argument="-window" type="integer" label="Size of the sliding window"
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108 min="0" optional="true"
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109 help="The default is unit_size + 4" />
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110 </section>
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111 </inputs>
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112 <outputs>
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113 <data name="mask_output" format="bed">
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114 <change_format>
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115 <when input="output_format" value="fasta" format="fasta" />
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116 <when input="output_format" value="maskinfo_asn1_bin" format="maskinfo-asn1-binary" />
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117 <when input="output_format" value="maskinfo_asn1_text" format="maskinfo-asn1" />
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118 <when input="output_format" value="maskinfo_xml" format="xml" />
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119 </change_format>
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120 </data>
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121 </outputs>
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122 <tests>
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123 <test>
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124 <!-- Test WindowMasker ustat with fasta output -->
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125 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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126 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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127 <param name="output_format" value="fasta" />
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128 <output name="mask_output" file="contigs.wm.fa" ftype="fasta" />
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129 </test>
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130 <test>
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131 <!-- Test WindowMasker ustat with ASN.1 text output -->
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132 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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133 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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134 <param name="output_format" value="maskinfo_asn1_text" />
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135 <output name="mask_output" file="contigs.wm.asn1" ftype="maskinfo-asn1"/>
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136 </test>
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137 <test>
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138 <!-- Test WindowMasker ustat with ASN.1 binary output -->
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139 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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140 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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141 <param name="output_format" value="maskinfo_asn1_bin" />
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142 <output name="mask_output" file="contigs.wm.asnb" ftype="maskinfo-asn1-binary"/>
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143 </test>
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144 <test>
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145 <!-- Test WindowMasker ustat with XML output -->
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146 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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147 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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148 <param name="output_format" value="maskinfo_xml" />
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149 <output name="mask_output" file="contigs.wm.xml" ftype="xml"/>
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150 </test>
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151 <test>
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152 <!-- Test WindowMasker ustat with advanced settings -->
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153 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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154 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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155 <param name="set_t_high" value="10" />
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156 <param name="set_t_low" value="9" />
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157 <param name="t_extend" value="5" />
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158 <param name="t_low" value="9" />
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159 <param name="t_high" value="10" />
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160 <param name="t_thres" value="20" />
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161 <param name="window" value="50" />
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162 <output name="mask_output" file="contigs.advanced_wm.bed" ftype="bed"/>
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163 </test>
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164 <test>
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165 <!-- Test WindowMasker ustat without dust -->
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166 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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167 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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168 <param name="use_dust_selector" value="no" />
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169 <output name="mask_output" file="contigs.nodust_wm.bed" ftype="bed"/>
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170 </test>
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171 <test>
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172 <!-- Test WindowMasker ustat with parse Seq-ids -->
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173 <param name="fasta_input" value="contigs.fa" ftype="fasta" />
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174 <param name="mkcount_input" value="chr4_part.oascii.counts" ftype="txt" />
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175 <param name="parse_seqids" value="-parse_seqids" />
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176 <output name="mask_output" file="contigs.seqid_wm.bed" ftype="bed"/>
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177 </test>
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178 </tests>
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179 <help>
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180 <![CDATA[
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181 **What it does**
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182
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183 This tool runs `stage 2 <https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/lxr/source/src/app/winmasker/>`_
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184 of the WindowMasker analysis to identify repeats within the input sequences.
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185
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186 .. class:: infomark
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187
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188 **Output formats:**
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189
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190 * Use the **binary or text maskinfo ASN.1** output formats to generate the mask file for
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191 the `NCBI BLAST+ makeblastdb tool <https://www.ncbi.nlm.nih.gov/books/NBK279681/#_cookbook_Create_BLAST_database_with_the_>`_
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192 * Use the BED output format to generate a list of masked regions
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193
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194
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195 .. class:: infomark
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196
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197 **Advanced options:**
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198
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199 * See the `WindowMasker README file <https://www.ncbi.nlm.nih.gov/IEB/ToolBox/CPP_DOC/lxr/source/src/app/winmasker/README>`_
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200 for additional details on the WindowMasker repeat masking options
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201
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202 ]]></help>
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203 <expand macro="citations" />
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204 </tool>