Mercurial > repos > jankanis > blast2html
annotate blast2html.py @ 74:03e044b5bcc2 py2.6
fix escaping of javascript literals
author | Jan Kanis <jan.code@jankanis.nl> |
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date | Wed, 18 Jun 2014 16:58:25 +0200 |
parents | 67b1a319c6dc |
children | 4d2c25baf5a3 |
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1 #!/usr/bin/env python3 |
50 | 2 # -*- coding: utf-8 -*- |
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3 |
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4 # Actually this program works with both python 2 and 3 |
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5 |
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6 # Copyright The Hyve B.V. 2014 |
72 | 7 # License: GPL version 3 or (at your option) any higher version |
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8 |
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9 from __future__ import unicode_literals, division |
50 | 10 |
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11 import sys |
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12 import math |
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13 import warnings |
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14 import six, codecs |
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15 from os import path |
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16 from itertools import repeat |
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17 import argparse |
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18 from lxml import objectify |
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19 import jinja2 |
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20 |
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21 |
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22 |
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23 _filters = dict() |
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24 def filter(func_or_name): |
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25 "Decorator to register a function as filter in the current jinja environment" |
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26 if isinstance(func_or_name, six.string_types): |
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27 def inner(func): |
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28 _filters[func_or_name] = func.__name__ |
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29 return func |
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30 return inner |
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31 else: |
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32 _filters[func_or_name.__name__] = func_or_name.__name__ |
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33 return func_or_name |
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34 |
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35 |
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36 def color_idx(length): |
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37 if length < 40: |
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38 return 0 |
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39 elif length < 50: |
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40 return 1 |
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41 elif length < 80: |
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42 return 2 |
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43 elif length < 200: |
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44 return 3 |
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45 return 4 |
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46 |
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47 @filter |
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48 def fmt(val, fmt): |
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49 return format(float(val), fmt) |
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50 |
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51 @filter |
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52 def firsttitle(hit): |
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53 return hit.Hit_def.text.split('>')[0] |
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54 |
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55 @filter |
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56 def othertitles(hit): |
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57 """Split a hit.Hit_def that contains multiple titles up, splitting out the hit ids from the titles.""" |
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58 id_titles = hit.Hit_def.text.split('>') |
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59 |
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60 titles = [] |
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61 for t in id_titles[1:]: |
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62 fullid, title = t.split(' ', 1) |
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63 hitid, id = fullid.split('|', 2)[1:3] |
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64 titles.append(dict(id = id, |
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65 hitid = hitid, |
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66 fullid = fullid, |
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67 title = title)) |
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68 return titles |
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69 |
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70 @filter |
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71 def hitid(hit): |
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72 hitid = hit.Hit_id.text |
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73 s = hitid.split('|', 2) |
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74 if len(s) >= 2: |
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75 return s[1] |
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76 return hitid |
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77 |
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78 @filter |
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79 def seqid(hit): |
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80 hitid = hit.Hit_id.text |
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81 s = hitid.split('|', 2) |
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82 if len(s) >= 3: |
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83 return s[2] |
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84 return hitid |
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85 |
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86 |
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87 @filter |
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88 def alignment_pre(hsp): |
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89 """Create the preformatted alignment blocks""" |
71 | 90 |
91 # line break length | |
92 linewidth = 60 | |
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93 |
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94 qfrom = int(hsp['Hsp_query-from']) |
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95 qto = int(hsp['Hsp_query-to']) |
69 | 96 qframe = int(hsp['Hsp_query-frame']) |
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97 hfrom = int(hsp['Hsp_hit-from']) |
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98 hto = int(hsp['Hsp_hit-to']) |
69 | 99 hframe = int(hsp['Hsp_hit-frame']) |
71 | 100 |
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101 qseq = hsp.Hsp_qseq.text |
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102 midline = hsp.Hsp_midline.text |
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103 hseq = hsp.Hsp_hseq.text |
69 | 104 |
71 | 105 if not qframe in (1, -1): |
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106 warnings.warn("Error in BlastXML input: Hsp node {0} has a Hsp_query-frame of {1}. (should be 1 or -1)".format(nodeid(hsp), qframe)) |
69 | 107 qframe = -1 if qframe < 0 else 1 |
71 | 108 if not hframe in (1, -1): |
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109 warnings.warn("Error in BlastXML input: Hsp node {0} has a Hsp_hit-frame of {1}. (should be 1 or -1)".format(nodeid(hsp), hframe)) |
69 | 110 hframe = -1 if hframe < 0 else 1 |
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111 |
69 | 112 def split(txt): |
71 | 113 return [txt[i:i+linewidth] for i in range(0, len(txt), linewidth)] |
69 | 114 |
71 | 115 for qs, mid, hs, offset in zip(split(qseq), split(midline), split(hseq), range(0, len(qseq), linewidth)): |
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116 yield ( |
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117 "Query {0:>7} {1} {2}\n".format(qfrom+offset*qframe, qs, qfrom+(offset+len(qs)-1)*qframe) + |
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118 " {0:7} {1}\n".format('', mid) + |
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119 "Subject{0:>7} {1} {2}".format(hfrom+offset*hframe, hs, hfrom+(offset+len(hs)-1)*hframe) |
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120 ) |
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121 |
69 | 122 if qfrom+(len(qseq)-1)*qframe != qto: |
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123 warnings.warn("Error in BlastXML input: Hsp node {0} qseq length mismatch: from {1} to {2} length {3}".format( |
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124 nodeid(hsp), qfrom, qto, len(qseq))) |
69 | 125 if hfrom+(len(hseq)-1)*hframe != hto: |
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126 warnings.warn("Error in BlastXML input: Hsp node {0} hseq length mismatch: from {1} to {2} length {3}".format( |
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127 nodeid(hsp), hfrom, hto, len(hseq))) |
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128 |
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129 |
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130 |
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131 @filter('len') |
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132 def blastxml_len(node): |
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133 if node.tag == 'Hsp': |
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134 return int(node['Hsp_align-len']) |
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135 elif node.tag == 'Iteration': |
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136 return int(node['Iteration_query-len']) |
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137 raise Exception("Unknown XML node type: "+node.tag) |
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138 |
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139 @filter |
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140 def nodeid(node): |
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141 id = [] |
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142 if node.tag == 'Hsp': |
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143 id.insert(0, node.Hsp_num.text) |
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144 node = node.getparent().getparent() |
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145 assert node.tag == 'Hit' |
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146 if node.tag == 'Hit': |
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147 id.insert(0, node.Hit_num.text) |
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148 node = node.getparent().getparent() |
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149 assert node.tag == 'Iteration' |
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150 if node.tag == 'Iteration': |
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151 id.insert(0, node['Iteration_iter-num'].text) |
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152 return '-'.join(id) |
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153 raise ValueError("The nodeid filter can only be applied to Hsp, Hit or Iteration nodes in a BlastXML document") |
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154 |
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155 |
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156 @filter |
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157 def asframe(frame): |
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158 if frame == 1: |
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159 return 'Plus' |
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160 elif frame == -1: |
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161 return 'Minus' |
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162 raise Exception("frame should be either +1 or -1") |
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163 |
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164 def genelink(hit, type='genbank', hsp=None): |
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165 if not isinstance(hit, six.string_types): |
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166 hit = hitid(hit) |
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167 link = "http://www.ncbi.nlm.nih.gov/nucleotide/{0}?report={1}&log$=nuclalign".format(hit, type) |
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168 if hsp != None: |
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169 link += "&from={0}&to={1}".format(hsp['Hsp_hit-from'], hsp['Hsp_hit-to']) |
16 | 170 return link |
171 | |
172 | |
173 # javascript escape filter based on Django's, from https://github.com/dsissitka/khan-website/blob/master/templatefilters.py#L112-139 | |
174 # I've removed the html escapes, since html escaping is already being performed by the template engine. | |
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175 |
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176 # The r'\u0027' syntax doesn't work the way we need to in python 2.6 with unicode_literals |
16 | 177 _base_js_escapes = ( |
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178 ('\\', '\\u005C'), |
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179 ('\'', '\\u0027'), |
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180 ('"', '\\u0022'), |
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181 # ('>', '\\u003E'), |
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182 # ('<', '\\u003C'), |
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183 # ('&', '\\u0026'), |
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184 # ('=', '\\u003D'), |
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185 # ('-', '\\u002D'), |
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186 # (';', '\\u003B'), |
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187 (u'\u2028', '\\u2028'), |
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188 (u'\u2029', '\\u2029') |
16 | 189 ) |
190 | |
191 # Escape every ASCII character with a value less than 32. This is | |
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192 # needed a.o. to prevent html parsers from jumping out of javascript |
16 | 193 # parsing mode. |
194 _js_escapes = (_base_js_escapes + | |
195 tuple(('%c' % z, '\\u%04X' % z) for z in range(32))) | |
196 | |
197 @filter | |
198 def js_string_escape(value): | |
72 | 199 """ |
200 Javascript string literal escape. Note that this only escapes data | |
201 for embedding within javascript string literals, not in general | |
202 javascript snippets. | |
203 """ | |
16 | 204 |
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205 value = six.text_type(value) |
16 | 206 |
207 for bad, good in _js_escapes: | |
208 value = value.replace(bad, good) | |
209 | |
210 return value | |
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211 |
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212 @filter |
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213 def hits(result): |
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214 # sort hits by longest hotspot first |
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215 return sorted(result.Iteration_hits.findall('Hit'), |
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216 key=lambda h: max(blastxml_len(hsp) for hsp in h.Hit_hsps.Hsp), |
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217 reverse=True) |
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218 |
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219 |
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220 |
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221 class BlastVisualize: |
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222 |
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223 colors = ('black', 'blue', 'green', 'magenta', 'red') |
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224 |
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225 max_scale_labels = 10 |
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226 |
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227 def __init__(self, input, templatedir, templatename): |
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228 self.input = input |
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229 self.templatename = templatename |
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230 |
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231 self.blast = objectify.parse(self.input).getroot() |
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232 self.loader = jinja2.FileSystemLoader(searchpath=templatedir, encoding='utf-8') |
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233 self.environment = jinja2.Environment(loader=self.loader, |
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234 lstrip_blocks=True, trim_blocks=True, autoescape=True) |
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235 |
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236 self._addfilters(self.environment) |
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237 |
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238 |
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239 def _addfilters(self, environment): |
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240 for filtername, funcname in _filters.items(): |
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241 try: |
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242 environment.filters[filtername] = getattr(self, funcname) |
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243 except AttributeError: |
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244 environment.filters[filtername] = globals()[funcname] |
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245 |
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246 def render(self, output): |
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247 template = self.environment.get_template(self.templatename) |
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248 |
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249 params = (('Query ID', self.blast["BlastOutput_query-ID"]), |
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250 ('Query definition', self.blast["BlastOutput_query-def"]), |
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251 ('Query length', self.blast["BlastOutput_query-len"]), |
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252 ('Program', self.blast.BlastOutput_version), |
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253 ('Database', self.blast.BlastOutput_db), |
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254 ) |
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255 |
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256 result = template.render(blast=self.blast, |
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257 iterations=self.blast.BlastOutput_iterations.Iteration, |
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258 colors=self.colors, |
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259 # match_colors=self.match_colors(), |
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260 # hit_info=self.hit_info(), |
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261 genelink=genelink, |
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262 params=params) |
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263 if six.PY2: |
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264 result = result.encode('utf-8') |
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265 output.write(result) |
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266 |
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267 @filter |
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268 def match_colors(self, result): |
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269 """ |
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270 An iterator that yields lists of length-color pairs. |
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271 """ |
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272 |
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273 query_length = blastxml_len(result) |
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274 |
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275 percent_multiplier = 100 / query_length |
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276 |
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277 for hit in hits(result): |
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278 # sort hotspots from short to long, so we can overwrite index colors of |
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279 # short matches with those of long ones. |
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280 hotspots = sorted(hit.Hit_hsps.Hsp, key=lambda hsp: blastxml_len(hsp)) |
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281 table = bytearray([255]) * query_length |
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282 for hsp in hotspots: |
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283 frm = hsp['Hsp_query-from'] - 1 |
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284 to = int(hsp['Hsp_query-to']) |
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285 table[frm:to] = repeat(color_idx(blastxml_len(hsp)), to - frm) |
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286 |
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287 matches = [] |
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288 last = table[0] |
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289 count = 0 |
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290 for i in range(query_length): |
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291 if table[i] == last: |
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292 count += 1 |
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293 continue |
16 | 294 matches.append((count * percent_multiplier, self.colors[last] if last != 255 else 'transparent')) |
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295 last = table[i] |
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296 count = 1 |
16 | 297 matches.append((count * percent_multiplier, self.colors[last] if last != 255 else 'transparent')) |
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298 |
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299 yield dict(colors=matches, hit=hit, defline=firsttitle(hit)) |
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300 |
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301 @filter |
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302 def queryscale(self, result): |
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303 query_length = blastxml_len(result) |
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304 skip = math.ceil(query_length / self.max_scale_labels) |
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305 percent_multiplier = 100 / query_length |
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306 for i in range(1, query_length+1): |
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307 if i % skip == 0: |
23 | 308 yield dict(label = i, width = skip * percent_multiplier) |
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309 if query_length % skip != 0: |
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310 yield dict(label = query_length, |
23 | 311 width = (query_length % skip) * percent_multiplier) |
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312 |
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313 @filter |
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314 def hit_info(self, result): |
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315 |
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316 query_length = blastxml_len(result) |
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317 |
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318 for hit in hits(result): |
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319 hsps = hit.Hit_hsps.Hsp |
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320 |
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321 cover = [False] * query_length |
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322 for hsp in hsps: |
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323 cover[hsp['Hsp_query-from']-1 : int(hsp['Hsp_query-to'])] = repeat(True, blastxml_len(hsp)) |
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324 cover_count = cover.count(True) |
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325 |
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326 def hsp_val(path): |
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327 return (float(hsp[path]) for hsp in hsps) |
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328 |
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329 yield dict(hit = hit, |
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330 title = firsttitle(hit), |
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331 maxscore = "{0:.1f}".format(max(hsp_val('Hsp_bit-score'))), |
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332 totalscore = "{0:.1f}".format(sum(hsp_val('Hsp_bit-score'))), |
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333 cover = "{0:.0%}".format(cover_count / query_length), |
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334 e_value = "{0:.4g}".format(min(hsp_val('Hsp_evalue'))), |
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335 # FIXME: is this the correct formula vv? |
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336 ident = "{0:.0%}".format(float(min(hsp.Hsp_identity / blastxml_len(hsp) for hsp in hsps))), |
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337 accession = hit.Hit_accession) |
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338 |
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339 |
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340 def main(): |
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341 default_template = path.join(path.dirname(__file__), 'blast2html.html.jinja') |
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342 |
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343 parser = argparse.ArgumentParser(description="Convert a BLAST XML result into a nicely readable html page", |
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344 usage="{0} [-i] INPUT [-o OUTPUT]".format(sys.argv[0])) |
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345 input_group = parser.add_mutually_exclusive_group(required=True) |
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346 input_group.add_argument('positional_arg', metavar='INPUT', nargs='?', type=argparse.FileType(mode='r'), |
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347 help='The input Blast XML file, same as -i/--input') |
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348 input_group.add_argument('-i', '--input', type=argparse.FileType(mode='r'), |
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349 help='The input Blast XML file') |
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350 parser.add_argument('-o', '--output', type=argparse.FileType(mode='w'), default=sys.stdout, |
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351 help='The output html file') |
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352 # We just want the file name here, so jinja can open the file |
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353 # itself. But it is easier to just use a FileType so argparse can |
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354 # handle the errors. This introduces a small race condition when |
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355 # jinja later tries to re-open the template file, but we don't |
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356 # care too much. |
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357 parser.add_argument('--template', type=argparse.FileType(mode='r'), default=default_template, |
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358 help='The template file to use. Defaults to blast_html.html.jinja') |
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359 |
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360 args = parser.parse_args() |
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361 if args.input == None: |
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362 args.input = args.positional_arg |
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363 if args.input == None: |
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364 parser.error('no input specified') |
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365 |
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366 templatedir, templatename = path.split(args.template.name) |
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367 args.template.close() |
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368 if not templatedir: |
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369 templatedir = '.' |
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370 |
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371 b = BlastVisualize(args.input, templatedir, templatename) |
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372 b.render(args.output) |
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373 |
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374 |
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375 if __name__ == '__main__': |
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376 main() |
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377 |