Mercurial > repos > jankanis > blast2html
annotate blast2html.py @ 35:2b23b4a4d88e
fix tool xml file name
author | Jan Kanis <jan.code@jankanis.nl> |
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date | Thu, 15 May 2014 18:15:04 +0200 |
parents | 4e6ac737ba17 |
children | bfc82a8aa3c9 |
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1 #!/usr/bin/env python3 |
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2 # Actually runs under either python 2 or 3 |
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3 |
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4 # Copyright The Hyve B.V. 2014 |
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5 # License: GPL version 3 or higher |
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6 |
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7 import sys |
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8 import math |
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9 import warnings |
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10 from os import path |
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11 from itertools import repeat |
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12 import argparse |
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13 from lxml import objectify |
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14 import jinja2 |
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15 |
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16 |
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17 |
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18 _filters = {} |
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19 def filter(func_or_name): |
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20 "Decorator to register a function as filter in the current jinja environment" |
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21 if isinstance(func_or_name, str): |
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22 def inner(func): |
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23 _filters[func_or_name] = func.__name__ |
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24 return func |
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25 return inner |
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26 else: |
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27 _filters[func_or_name.__name__] = func_or_name.__name__ |
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28 return func_or_name |
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29 |
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30 |
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31 def color_idx(length): |
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32 if length < 40: |
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33 return 0 |
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34 elif length < 50: |
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35 return 1 |
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36 elif length < 80: |
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37 return 2 |
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38 elif length < 200: |
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39 return 3 |
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40 return 4 |
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41 |
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42 @filter |
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43 def fmt(val, fmt): |
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44 return format(float(val), fmt) |
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45 |
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46 @filter |
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47 def firsttitle(hit): |
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48 return hit.Hit_def.text.split('>')[0] |
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49 |
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50 @filter |
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51 def othertitles(hit): |
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52 """Split a hit.Hit_def that contains multiple titles up, splitting out the hit ids from the titles.""" |
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53 id_titles = hit.Hit_def.text.split('>') |
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54 |
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55 titles = [] |
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56 for t in id_titles[1:]: |
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57 fullid, title = t.split(' ', 1) |
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58 hitid, id = fullid.split('|', 2)[1:3] |
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59 titles.append(dict(id = id, |
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60 hitid = hitid, |
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61 fullid = fullid, |
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62 title = title)) |
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63 return titles |
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64 |
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65 @filter |
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66 def hitid(hit): |
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67 hitid = hit.Hit_id.text |
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68 s = hitid.split('|', 2) |
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69 if len(s) >= 2: |
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70 return s[1] |
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71 return hitid |
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72 |
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73 @filter |
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74 def seqid(hit): |
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75 hitid = hit.Hit_id.text |
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76 s = hitid.split('|', 2) |
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77 if len(s) >= 3: |
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78 return s[2] |
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79 return hitid |
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80 |
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81 |
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82 @filter |
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83 def alignment_pre(hsp): |
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84 return ( |
15 | 85 "Query {:>7s} {} {}\n".format(hsp['Hsp_query-from'].text, hsp.Hsp_qseq, hsp['Hsp_query-to']) + |
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86 " {:7s} {}\n".format('', hsp.Hsp_midline) + |
15 | 87 "Subject{:>7s} {} {}".format(hsp['Hsp_hit-from'].text, hsp.Hsp_hseq, hsp['Hsp_hit-to']) |
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88 ) |
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89 |
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90 @filter('len') |
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91 def blastxml_len(node): |
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92 if node.tag == 'Hsp': |
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93 return int(node['Hsp_align-len']) |
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94 elif node.tag == 'Iteration': |
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95 return int(node['Iteration_query-len']) |
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96 raise Exception("Unknown XML node type: "+node.tag) |
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97 |
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98 |
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99 @filter |
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100 def asframe(frame): |
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101 if frame == 1: |
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102 return 'Plus' |
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103 elif frame == -1: |
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104 return 'Minus' |
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105 raise Exception("frame should be either +1 or -1") |
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106 |
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107 def genelink(hit, type='genbank', hsp=None): |
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108 if not isinstance(hit, str): |
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109 hit = hitid(hit) |
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110 link = "http://www.ncbi.nlm.nih.gov/nucleotide/{}?report={}&log$=nuclalign".format(hit, type) |
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111 if hsp != None: |
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112 link += "&from={}&to={}".format(hsp['Hsp_hit-from'], hsp['Hsp_hit-to']) |
16 | 113 return link |
114 | |
115 | |
116 # javascript escape filter based on Django's, from https://github.com/dsissitka/khan-website/blob/master/templatefilters.py#L112-139 | |
117 # I've removed the html escapes, since html escaping is already being performed by the template engine. | |
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118 |
16 | 119 _base_js_escapes = ( |
120 ('\\', r'\u005C'), | |
121 ('\'', r'\u0027'), | |
122 ('"', r'\u0022'), | |
123 # ('>', r'\u003E'), | |
124 # ('<', r'\u003C'), | |
125 # ('&', r'\u0026'), | |
126 # ('=', r'\u003D'), | |
127 # ('-', r'\u002D'), | |
128 # (';', r'\u003B'), | |
129 # (u'\u2028', r'\u2028'), | |
130 # (u'\u2029', r'\u2029') | |
131 ) | |
132 | |
133 # Escape every ASCII character with a value less than 32. This is | |
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134 # needed a.o. to prevent html parsers from jumping out of javascript |
16 | 135 # parsing mode. |
136 _js_escapes = (_base_js_escapes + | |
137 tuple(('%c' % z, '\\u%04X' % z) for z in range(32))) | |
138 | |
139 @filter | |
140 def js_string_escape(value): | |
141 """Escape javascript string literal escapes. Note that this only works | |
142 within javascript string literals, not in general javascript | |
143 snippets.""" | |
144 | |
145 value = str(value) | |
146 | |
147 for bad, good in _js_escapes: | |
148 value = value.replace(bad, good) | |
149 | |
150 return value | |
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151 |
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152 @filter |
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153 def hits(result): |
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154 # sort hits by longest hotspot first |
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155 return sorted(result.Iteration_hits.findall('Hit'), |
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156 key=lambda h: max(blastxml_len(hsp) for hsp in h.Hit_hsps.Hsp), |
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157 reverse=True) |
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158 |
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159 |
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160 |
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161 class BlastVisualize: |
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162 |
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163 colors = ('black', 'blue', 'green', 'magenta', 'red') |
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164 |
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165 max_scale_labels = 10 |
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166 |
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167 def __init__(self, input, templatedir, templatename): |
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168 self.input = input |
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169 self.templatename = templatename |
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170 |
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171 self.blast = objectify.parse(self.input).getroot() |
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172 self.loader = jinja2.FileSystemLoader(searchpath=templatedir) |
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173 self.environment = jinja2.Environment(loader=self.loader, |
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174 lstrip_blocks=True, trim_blocks=True, autoescape=True) |
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175 |
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176 self._addfilters(self.environment) |
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177 |
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178 |
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179 def _addfilters(self, environment): |
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180 for filtername, funcname in _filters.items(): |
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181 try: |
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182 environment.filters[filtername] = getattr(self, funcname) |
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183 except AttributeError: |
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184 environment.filters[filtername] = globals()[funcname] |
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185 |
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186 def render(self, output): |
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187 template = self.environment.get_template(self.templatename) |
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188 |
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189 params = (('Query ID', self.blast["BlastOutput_query-ID"]), |
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190 ('Query definition', self.blast["BlastOutput_query-def"]), |
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191 ('Query length', self.blast["BlastOutput_query-len"]), |
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192 ('Program', self.blast.BlastOutput_version), |
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193 ('Database', self.blast.BlastOutput_db), |
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194 ) |
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195 |
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196 output.write(template.render(blast=self.blast, |
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197 iterations=self.blast.BlastOutput_iterations.Iteration, |
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198 colors=self.colors, |
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199 # match_colors=self.match_colors(), |
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200 # hit_info=self.hit_info(), |
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201 genelink=genelink, |
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202 params=params)) |
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203 |
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204 @filter |
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205 def match_colors(self, result): |
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206 """ |
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207 An iterator that yields lists of length-color pairs. |
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208 """ |
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209 |
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210 query_length = blastxml_len(result) |
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211 |
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212 percent_multiplier = 100 / query_length |
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213 |
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214 for hit in hits(result): |
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215 # sort hotspots from short to long, so we can overwrite index colors of |
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216 # short matches with those of long ones. |
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217 hotspots = sorted(hit.Hit_hsps.Hsp, key=lambda hsp: blastxml_len(hsp)) |
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218 table = bytearray([255]) * query_length |
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219 for hsp in hotspots: |
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220 frm = hsp['Hsp_query-from'] - 1 |
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221 to = int(hsp['Hsp_query-to']) |
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222 table[frm:to] = repeat(color_idx(blastxml_len(hsp)), to - frm) |
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223 |
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224 matches = [] |
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225 last = table[0] |
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226 count = 0 |
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227 for i in range(query_length): |
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228 if table[i] == last: |
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229 count += 1 |
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230 continue |
16 | 231 matches.append((count * percent_multiplier, self.colors[last] if last != 255 else 'transparent')) |
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232 last = table[i] |
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233 count = 1 |
16 | 234 matches.append((count * percent_multiplier, self.colors[last] if last != 255 else 'transparent')) |
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235 |
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236 yield dict(colors=matches, link="#hit"+hit.Hit_num.text, defline=firsttitle(hit)) |
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237 |
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238 @filter |
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239 def queryscale(self, result): |
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240 query_length = blastxml_len(result) |
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241 skip = math.ceil(query_length / self.max_scale_labels) |
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242 percent_multiplier = 100 / query_length |
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243 for i in range(1, query_length+1): |
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244 if i % skip == 0: |
23 | 245 yield dict(label = i, width = skip * percent_multiplier) |
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246 if query_length % skip != 0: |
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247 yield dict(label = query_length, |
23 | 248 width = (query_length % skip) * percent_multiplier) |
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249 |
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250 @filter |
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251 def hit_info(self, result): |
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252 |
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253 query_length = blastxml_len(result) |
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254 |
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255 for hit in hits(result): |
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256 hsps = hit.Hit_hsps.Hsp |
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257 |
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258 cover = [False] * query_length |
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259 for hsp in hsps: |
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260 cover[hsp['Hsp_query-from']-1 : int(hsp['Hsp_query-to'])] = repeat(True, blastxml_len(hsp)) |
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261 cover_count = cover.count(True) |
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262 |
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263 def hsp_val(path): |
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264 return (float(hsp[path]) for hsp in hsps) |
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265 |
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266 yield dict(hit = hit, |
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267 title = firsttitle(hit), |
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268 link_id = hit.Hit_num, |
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269 maxscore = "{:.1f}".format(max(hsp_val('Hsp_bit-score'))), |
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270 totalscore = "{:.1f}".format(sum(hsp_val('Hsp_bit-score'))), |
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271 cover = "{:.0%}".format(cover_count / query_length), |
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272 e_value = "{:.4g}".format(min(hsp_val('Hsp_evalue'))), |
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273 # FIXME: is this the correct formula vv? |
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274 ident = "{:.0%}".format(float(min(hsp.Hsp_identity / blastxml_len(hsp) for hsp in hsps))), |
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275 accession = hit.Hit_accession) |
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276 |
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277 |
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278 def main(): |
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279 default_template = path.join(path.dirname(__file__), 'blast2html.html.jinja') |
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280 |
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281 parser = argparse.ArgumentParser(description="Convert a BLAST XML result into a nicely readable html page", |
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282 usage="{} [-i] INPUT [-o OUTPUT]".format(sys.argv[0])) |
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283 input_group = parser.add_mutually_exclusive_group(required=True) |
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284 input_group.add_argument('positional_arg', metavar='INPUT', nargs='?', type=argparse.FileType(mode='r'), |
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285 help='The input Blast XML file, same as -i/--input') |
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286 input_group.add_argument('-i', '--input', type=argparse.FileType(mode='r'), |
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287 help='The input Blast XML file') |
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288 parser.add_argument('-o', '--output', type=argparse.FileType(mode='w'), default=sys.stdout, |
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289 help='The output html file') |
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290 # We just want the file name here, so jinja can open the file |
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291 # itself. But it is easier to just use a FileType so argparse can |
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292 # handle the errors. This introduces a small race condition when |
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293 # jinja later tries to re-open the template file, but we don't |
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294 # care too much. |
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295 parser.add_argument('--template', type=argparse.FileType(mode='r'), default=default_template, |
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296 help='The template file to use. Defaults to blast_html.html.jinja') |
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297 |
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298 args = parser.parse_args() |
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299 if args.input == None: |
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300 args.input = args.positional_arg |
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301 if args.input == None: |
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302 parser.error('no input specified') |
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303 |
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304 templatedir, templatename = path.split(args.template.name) |
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305 args.template.close() |
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306 if not templatedir: |
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307 templatedir = '.' |
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308 |
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309 b = BlastVisualize(args.input, templatedir, templatename) |
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310 b.render(args.output) |
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311 |
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312 |
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313 if __name__ == '__main__': |
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314 main() |
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315 |