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2 <html>
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3 <head>
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4 <meta charset="UTF-8">
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5 <meta name=generator content="blast2html; see https://github.com/thehyve/blast2html/">
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6
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7 <title>Blast output</title>
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8
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9 <style>
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10 body {
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11 color: #333333;
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19 .right {
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20 float: right;
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21 }
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22
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23 /* Galaxy with html sanitization enabled strips the header of this html page. If so, show the user a warning.*/
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24 #strip_html_warning {
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25 display: none;
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26 }
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28 #content {
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29 margin: 0 2em;
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30 padding: 0.5em;
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31 border: 1px solid #888888;
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354 footer :link {
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355 color: #5588cc;
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356 }
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357
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358 </style>
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359
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360 <script type="text/javascript">
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361 function toggle_visibility(id) {
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362 var e = document.getElementById(id);
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363 if(e.style.display != 'block')
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364 e.style.display = 'block';
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365 else
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366 e.style.display = 'none';
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367 }
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368 </script>
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369
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370 </head>
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371
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372
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373 <body>
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374
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375 <div id="strip_html_warning">
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376 <!-- This div should be hidden by the header css block. Galaxy
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377 strips all css, breaking this page but making this warning
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378 visible. This warning contains some ugly old skool tabular html
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379 layout that is not stripped. -->
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380 <table bgcolor="#FFE5C9"><tr><td><font color="red"><b>
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381 <font size=5><center>This html page seems to have been stripped by Galaxy.</center></font>
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382 Disable Galaxy's html sanitization feature to view the full page (see <font face=monospace>sanitize_all_html</font> in your galaxy's universe_wsgi.ini), or download this page instead of viewing it in Galaxy.
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383 </b></font></td></tr></table>
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384 </div>
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385
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386 <div id=content>
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387
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388
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389 <section class=index>
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390 <h1>Queries</h1>
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391
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392 <div class=indexentry><a href="#match1">
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393 Query_1: Forward_Primer|NOST-Spec_(Genetic_ID)
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394 (20 letters, 2 hits)
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395 </a></div>
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396 <div class=indexentry><a href="#match2">
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397 Query_2: Reverse_Primer|NOST-Spec_(Genetic_ID)
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398 (20 letters, 0 hits)
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399 </a></div>
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400 <div class=indexentry><a href="#match3">
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401 Query_3: Probe|NOST-Spec_(Genetic_ID)
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402 (20 letters, 3 hits)
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403 </a></div>
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404 <div class=indexentry><a href="#match4">
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405 Query_4: Forward_Primer|QL-ELE-Bt-F1(mod)/Bt-R
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406 (24 letters, 0 hits)
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407 </a></div>
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408 <div class=indexentry><a href="#match5">
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409 Query_5: Reverse_Primer|QL-ELE-Bt-F1(mod)/Bt-R
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410 (20 letters, 0 hits)
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411 </a></div>
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412 <div class=indexentry><a href="#match6">
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413 Query_6: Probe|QL-ELE-Bt-F1(mod)/Bt-R
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414 (25 letters, 2 hits)
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415 </a></div>
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416 <div class=indexentry><a href="#match7">
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417 Query_7: Amplicon|QL-ELE-Bt-F1(mod)/Bt-R
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418 (74 letters, 2 hits)
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419 </a></div>
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420
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421 </section>
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422
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423
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424 <section class=match id=match1>
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425
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426 <h1>Nucleotide Sequence (20 letters)</h1>
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427
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428 <section class=header>
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429
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430 <table class=headerdata>
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431 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
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432 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
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433 <tr><td class=param>Query length:</td><td>20</td></tr>
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434 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
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435 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
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436 </table>
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437
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438 </section>
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439
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440
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441 <section class=graphics>
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442 <h2>Graphic Summary</h2>
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443
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444 <div class=grey>
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445 <h3 class=centered>Distribution of 7 Blast Hits on the Query Sequence</h3>
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446
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447 <div class=defline id=defline1>
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448 Mouse-over to show defline and scores, click to show alignments
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449 </div>
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450
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451 <div class=graphic>
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452 <h4 class=darkHeader>Color key for alignment scores</h4>
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453 <div class=legend><div class=graphicrow>
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454 <div class=graphicitem style="background-color: black"><40</div>
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455 <div class=graphicitem style="background-color: blue">40–50</div>
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456 <div class=graphicitem style="background-color: green">50–80</div>
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457 <div class=graphicitem style="background-color: magenta">80–200</div>
|
|
|
458 <div class=graphicitem style="background-color: red">200≤</div>
|
|
|
459 </div></div>
|
|
|
460 <div style="clear: left"></div>
|
|
|
461
|
|
|
462 <div class=tablewrapper>
|
|
|
463
|
|
|
464 <div class=scale>
|
|
|
465 <div>query:</div>
|
|
|
466 <div class=graphicrow>
|
|
|
467 <div>
|
|
|
468 <div class=graphicitem style="width: 10%">
|
|
|
469 <div>2</div>
|
|
|
470 </div>
|
|
|
471 <div class=graphicitem style="width: 10%">
|
|
|
472 <div>4</div>
|
|
|
473 </div>
|
|
|
474 <div class=graphicitem style="width: 10%">
|
|
|
475 <div>6</div>
|
|
|
476 </div>
|
|
|
477 <div class=graphicitem style="width: 10%">
|
|
|
478 <div>8</div>
|
|
|
479 </div>
|
|
|
480 <div class=graphicitem style="width: 10%">
|
|
|
481 <div>10</div>
|
|
|
482 </div>
|
|
|
483 <div class=graphicitem style="width: 10%">
|
|
|
484 <div>12</div>
|
|
|
485 </div>
|
|
|
486 <div class=graphicitem style="width: 10%">
|
|
|
487 <div>14</div>
|
|
|
488 </div>
|
|
|
489 <div class=graphicitem style="width: 10%">
|
|
|
490 <div>16</div>
|
|
|
491 </div>
|
|
|
492 <div class=graphicitem style="width: 10%">
|
|
|
493 <div>18</div>
|
|
|
494 </div>
|
|
|
495 <div class=graphicitem style="width: 10%">
|
|
|
496 <div>20</div>
|
|
|
497 </div>
|
|
|
498 </div>
|
|
|
499 </div>
|
|
|
500 <div style="clear: left"></div>
|
|
|
501 </div>
|
|
|
502
|
|
|
503 <a class=matchresult
|
|
|
504 href="#hit1-1"
|
|
|
505 onmouseover='document.getElementById("defline1").innerHTML="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000"'
|
|
|
506 onmouseout='document.getElementById("defline1").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
507 title="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000">
|
|
|
508 <div class="matchrow graphicrow">
|
|
|
509 <div class="matchitem graphicitem"
|
|
|
510 style="background-color: black; width: 100%"></div>
|
|
|
511 </div>
|
|
|
512 </a>
|
|
|
513
|
|
|
514 <a class=matchresult
|
|
|
515 href="#hit1-2"
|
|
|
516 onmouseover='document.getElementById("defline1").innerHTML="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000"'
|
|
|
517 onmouseout='document.getElementById("defline1").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
518 title="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000">
|
|
|
519 <div class="matchrow graphicrow">
|
|
|
520 <div class="matchitem graphicitem"
|
|
|
521 style="background-color: black; width: 100%"></div>
|
|
|
522 </div>
|
|
|
523 </a>
|
|
|
524
|
|
|
525 </div>
|
|
|
526 </div>
|
|
|
527 </div>
|
|
|
528 </section>
|
|
|
529
|
|
|
530
|
|
|
531
|
|
|
532 <section class=descriptions>
|
|
|
533 <h2>Descriptions</h2>
|
|
|
534
|
|
|
535 <div class=grey><div class=white>
|
|
|
536 <h4 class=darkHeader>Sequences producing significant alignments:</h4>
|
|
|
537
|
|
|
538 <table class=descriptiontable>
|
|
|
539 <col/><col/><col/><col/><col/><col/><col/>
|
|
|
540 <tr>
|
|
|
541 <th>Description</th>
|
|
|
542 <th>Max score</th>
|
|
|
543 <th>Total score</th>
|
|
|
544 <th>Query cover</th>
|
|
|
545 <th>E value</th>
|
|
|
546 <th>Ident</th>
|
|
|
547 <th>Accession</th>
|
|
|
548 </tr>
|
|
|
549 <tr>
|
|
|
550 <td><div><a href="#hit1-1"
|
|
|
551 title="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000"
|
|
|
552 id="description1-1">
|
|
|
553 AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000
|
|
|
554 </a></div></td>
|
|
|
555 <td>40.1</td>
|
|
|
556 <td>40.1</td>
|
|
|
557 <td>100%</td>
|
|
|
558 <td>1.513e-07</td>
|
|
|
559 <td>100%</td>
|
|
|
560 <td><a href="http://example.com/example-genebank/AB209952.1/">5</a></td>
|
|
|
561 </tr>
|
|
|
562 <tr>
|
|
|
563 <td><div><a href="#hit1-2"
|
|
|
564 title="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000"
|
|
|
565 id="description1-2">
|
|
|
566 DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000
|
|
|
567 </a></div></td>
|
|
|
568 <td>40.1</td>
|
|
|
569 <td>40.1</td>
|
|
|
570 <td>100%</td>
|
|
|
571 <td>1.513e-07</td>
|
|
|
572 <td>100%</td>
|
|
|
573 <td><a href="http://example.com/example-genebank/DJ437711/">2</a></td>
|
|
|
574 </tr>
|
|
|
575 </table>
|
|
|
576
|
|
|
577 </div></div>
|
|
|
578 </section>
|
|
|
579
|
|
|
580
|
|
|
581
|
|
|
582 <section class=alignments>
|
|
|
583 <h2>Alignments</h2>
|
|
|
584
|
|
|
585 <div class=grey><div class=white>
|
|
|
586 <div class=alignment id=hit1-1>
|
|
|
587
|
|
|
588 <div class=linkheader>
|
|
|
589 <div class=right><a href="#description1-1">Descriptions</a></div>
|
|
|
590 <a class="linkheader" href="http://example.com/example-genebank/AB209952.1/">Gene Bank</a>
|
|
|
591 </div>
|
|
|
592
|
|
|
593 <div class=title>
|
|
|
594 <p class=hittitle>AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000</p>
|
|
|
595 <p class=titleinfo>
|
|
|
596 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/AB209952.1/">gnl|BL_ORD_ID|5</a>
|
|
|
597 <span class=b>Length:</span> 2457
|
|
|
598 <span class=b>Number of Matches:</span> 1
|
|
|
599 </p>
|
|
|
600 </div>
|
|
|
601
|
|
|
602
|
|
|
603 <div class=hotspot id=hotspot1-1-1>
|
|
|
604 <p class=range>
|
|
|
605 <span class=range>Range 1: 2119 to 2138</span>
|
|
|
606 </p>
|
|
|
607
|
|
|
608 <table class=hotspotstable>
|
|
|
609 <tr>
|
|
|
610 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
611 </tr>
|
|
|
612 <tr>
|
|
|
613 <td>40.1 bits(20)</td>
|
|
|
614 <td>0.0</td>
|
|
|
615 <td>20/20(100%)</td>
|
|
|
616 <td>0/20(0%)</td>
|
|
|
617 <td>Plus/Plus</td>
|
|
|
618 </tr>
|
|
|
619 </table>
|
|
|
620
|
|
|
621 <pre class=alignmentgraphic>Query 1 AGCGCGCAAACTAGGATAAA 20
|
|
|
622 ||||||||||||||||||||
|
|
|
623 Subject 2119 AGCGCGCAAACTAGGATAAA 2138</pre>
|
|
|
624 </div>
|
|
|
625
|
|
|
626 </div>
|
|
|
627
|
|
|
628 <div class=alignment id=hit1-2>
|
|
|
629
|
|
|
630 <div class=linkheader>
|
|
|
631 <div class=right><a href="#description1-2">Descriptions</a></div>
|
|
|
632 <a class="linkheader" href="http://example.com/example-genebank/DJ437711/">Gene Bank</a>
|
|
|
633 </div>
|
|
|
634
|
|
|
635 <div class=title>
|
|
|
636 <p class=hittitle>DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000</p>
|
|
|
637 <p class=titleinfo>
|
|
|
638 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/DJ437711/">gnl|BL_ORD_ID|2</a>
|
|
|
639 <span class=b>Length:</span> 323
|
|
|
640 <span class=b>Number of Matches:</span> 1
|
|
|
641 </p>
|
|
|
642 </div>
|
|
|
643
|
|
|
644
|
|
|
645 <div class=hotspot id=hotspot1-2-1>
|
|
|
646 <p class=range>
|
|
|
647 <span class=range>Range 1: 200 to 219</span>
|
|
|
648 </p>
|
|
|
649
|
|
|
650 <table class=hotspotstable>
|
|
|
651 <tr>
|
|
|
652 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
653 </tr>
|
|
|
654 <tr>
|
|
|
655 <td>40.1 bits(20)</td>
|
|
|
656 <td>0.0</td>
|
|
|
657 <td>20/20(100%)</td>
|
|
|
658 <td>0/20(0%)</td>
|
|
|
659 <td>Plus/Plus</td>
|
|
|
660 </tr>
|
|
|
661 </table>
|
|
|
662
|
|
|
663 <pre class=alignmentgraphic>Query 1 AGCGCGCAAACTAGGATAAA 20
|
|
|
664 ||||||||||||||||||||
|
|
|
665 Subject 200 AGCGCGCAAACTAGGATAAA 219</pre>
|
|
|
666 </div>
|
|
|
667
|
|
|
668 </div>
|
|
|
669
|
|
|
670 </div></div>
|
|
|
671 </section>
|
|
|
672 </section>
|
|
|
673
|
|
|
674 <section class=match id=match2>
|
|
|
675
|
|
|
676 <h1>Nucleotide Sequence (20 letters)</h1>
|
|
|
677
|
|
|
678 <section class=header>
|
|
|
679
|
|
|
680 <table class=headerdata>
|
|
|
681 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
682 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
683 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
684 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
685 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
686 </table>
|
|
|
687
|
|
|
688 </section>
|
|
|
689
|
|
|
690 <section>
|
|
|
691 <h2>No Hits</h2>
|
|
|
692 <div class=grey>
|
|
|
693 <table class=headerdata>
|
|
|
694 <tr><td class=param>Message:</td><td>No hits found</td></tr>
|
|
|
695 </table>
|
|
|
696 </div>
|
|
|
697 </section>
|
|
|
698 </section>
|
|
|
699
|
|
|
700 <section class=match id=match3>
|
|
|
701
|
|
|
702 <h1>Nucleotide Sequence (20 letters)</h1>
|
|
|
703
|
|
|
704 <section class=header>
|
|
|
705
|
|
|
706 <table class=headerdata>
|
|
|
707 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
708 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
709 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
710 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
711 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
712 </table>
|
|
|
713
|
|
|
714 </section>
|
|
|
715
|
|
|
716
|
|
|
717 <section class=graphics>
|
|
|
718 <h2>Graphic Summary</h2>
|
|
|
719
|
|
|
720 <div class=grey>
|
|
|
721 <h3 class=centered>Distribution of 7 Blast Hits on the Query Sequence</h3>
|
|
|
722
|
|
|
723 <div class=defline id=defline3>
|
|
|
724 Mouse-over to show defline and scores, click to show alignments
|
|
|
725 </div>
|
|
|
726
|
|
|
727 <div class=graphic>
|
|
|
728 <h4 class=darkHeader>Color key for alignment scores</h4>
|
|
|
729 <div class=legend><div class=graphicrow>
|
|
|
730 <div class=graphicitem style="background-color: black"><40</div>
|
|
|
731 <div class=graphicitem style="background-color: blue">40–50</div>
|
|
|
732 <div class=graphicitem style="background-color: green">50–80</div>
|
|
|
733 <div class=graphicitem style="background-color: magenta">80–200</div>
|
|
|
734 <div class=graphicitem style="background-color: red">200≤</div>
|
|
|
735 </div></div>
|
|
|
736 <div style="clear: left"></div>
|
|
|
737
|
|
|
738 <div class=tablewrapper>
|
|
|
739
|
|
|
740 <div class=scale>
|
|
|
741 <div>query:</div>
|
|
|
742 <div class=graphicrow>
|
|
|
743 <div>
|
|
|
744 <div class=graphicitem style="width: 10%">
|
|
|
745 <div>2</div>
|
|
|
746 </div>
|
|
|
747 <div class=graphicitem style="width: 10%">
|
|
|
748 <div>4</div>
|
|
|
749 </div>
|
|
|
750 <div class=graphicitem style="width: 10%">
|
|
|
751 <div>6</div>
|
|
|
752 </div>
|
|
|
753 <div class=graphicitem style="width: 10%">
|
|
|
754 <div>8</div>
|
|
|
755 </div>
|
|
|
756 <div class=graphicitem style="width: 10%">
|
|
|
757 <div>10</div>
|
|
|
758 </div>
|
|
|
759 <div class=graphicitem style="width: 10%">
|
|
|
760 <div>12</div>
|
|
|
761 </div>
|
|
|
762 <div class=graphicitem style="width: 10%">
|
|
|
763 <div>14</div>
|
|
|
764 </div>
|
|
|
765 <div class=graphicitem style="width: 10%">
|
|
|
766 <div>16</div>
|
|
|
767 </div>
|
|
|
768 <div class=graphicitem style="width: 10%">
|
|
|
769 <div>18</div>
|
|
|
770 </div>
|
|
|
771 <div class=graphicitem style="width: 10%">
|
|
|
772 <div>20</div>
|
|
|
773 </div>
|
|
|
774 </div>
|
|
|
775 </div>
|
|
|
776 <div style="clear: left"></div>
|
|
|
777 </div>
|
|
|
778
|
|
|
779 <a class=matchresult
|
|
|
780 href="#hit3-1"
|
|
|
781 onmouseover='document.getElementById("defline3").innerHTML="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000"'
|
|
|
782 onmouseout='document.getElementById("defline3").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
783 title="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000">
|
|
|
784 <div class="matchrow graphicrow">
|
|
|
785 <div class="matchitem graphicitem"
|
|
|
786 style="background-color: black; width: 100%"></div>
|
|
|
787 </div>
|
|
|
788 </a>
|
|
|
789
|
|
|
790 <a class=matchresult
|
|
|
791 href="#hit3-2"
|
|
|
792 onmouseover='document.getElementById("defline3").innerHTML="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000"'
|
|
|
793 onmouseout='document.getElementById("defline3").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
794 title="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000">
|
|
|
795 <div class="matchrow graphicrow">
|
|
|
796 <div class="matchitem graphicitem"
|
|
|
797 style="background-color: black; width: 100%"></div>
|
|
|
798 </div>
|
|
|
799 </a>
|
|
|
800
|
|
|
801 <a class=matchresult
|
|
|
802 href="#hit3-3"
|
|
|
803 onmouseover='document.getElementById("defline3").innerHTML="AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3\u0027UTR/plant_junction_region.|-9105899556052450000"'
|
|
|
804 onmouseout='document.getElementById("defline3").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
805 title="AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3'UTR/plant_junction_region.|-9105899556052450000">
|
|
|
806 <div class="matchrow graphicrow">
|
|
|
807 <div class="matchitem graphicitem"
|
|
|
808 style="background-color: transparent; width: 15%"></div>
|
|
|
809 <div class="matchitem graphicitem"
|
|
|
810 style="background-color: black; width: 85%"></div>
|
|
|
811 </div>
|
|
|
812 </a>
|
|
|
813
|
|
|
814 </div>
|
|
|
815 </div>
|
|
|
816 </div>
|
|
|
817 </section>
|
|
|
818
|
|
|
819
|
|
|
820
|
|
|
821 <section class=descriptions>
|
|
|
822 <h2>Descriptions</h2>
|
|
|
823
|
|
|
824 <div class=grey><div class=white>
|
|
|
825 <h4 class=darkHeader>Sequences producing significant alignments:</h4>
|
|
|
826
|
|
|
827 <table class=descriptiontable>
|
|
|
828 <col/><col/><col/><col/><col/><col/><col/>
|
|
|
829 <tr>
|
|
|
830 <th>Description</th>
|
|
|
831 <th>Max score</th>
|
|
|
832 <th>Total score</th>
|
|
|
833 <th>Query cover</th>
|
|
|
834 <th>E value</th>
|
|
|
835 <th>Ident</th>
|
|
|
836 <th>Accession</th>
|
|
|
837 </tr>
|
|
|
838 <tr>
|
|
|
839 <td><div><a href="#hit3-1"
|
|
|
840 title="AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000"
|
|
|
841 id="description3-1">
|
|
|
842 AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000
|
|
|
843 </a></div></td>
|
|
|
844 <td>40.1</td>
|
|
|
845 <td>40.1</td>
|
|
|
846 <td>100%</td>
|
|
|
847 <td>1.513e-07</td>
|
|
|
848 <td>100%</td>
|
|
|
849 <td><a href="http://example.com/example-genebank/AB209952.1/">5</a></td>
|
|
|
850 </tr>
|
|
|
851 <tr>
|
|
|
852 <td><div><a href="#hit3-2"
|
|
|
853 title="DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000"
|
|
|
854 id="description3-2">
|
|
|
855 DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000
|
|
|
856 </a></div></td>
|
|
|
857 <td>40.1</td>
|
|
|
858 <td>40.1</td>
|
|
|
859 <td>100%</td>
|
|
|
860 <td>1.513e-07</td>
|
|
|
861 <td>100%</td>
|
|
|
862 <td><a href="http://example.com/example-genebank/DJ437711/">2</a></td>
|
|
|
863 </tr>
|
|
|
864 <tr>
|
|
|
865 <td><div><a href="#hit3-3"
|
|
|
866 title="AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3'UTR/plant_junction_region.|-9105899556052450000"
|
|
|
867 id="description3-3">
|
|
|
868 AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3'UTR/plant_junction_region.|-9105899556052450000
|
|
|
869 </a></div></td>
|
|
|
870 <td>34.2</td>
|
|
|
871 <td>34.2</td>
|
|
|
872 <td>85%</td>
|
|
|
873 <td>9.334e-06</td>
|
|
|
874 <td>100%</td>
|
|
|
875 <td><a href="http://example.com/example-genebank/AJ308515.1/">1</a></td>
|
|
|
876 </tr>
|
|
|
877 </table>
|
|
|
878
|
|
|
879 </div></div>
|
|
|
880 </section>
|
|
|
881
|
|
|
882
|
|
|
883
|
|
|
884 <section class=alignments>
|
|
|
885 <h2>Alignments</h2>
|
|
|
886
|
|
|
887 <div class=grey><div class=white>
|
|
|
888 <div class=alignment id=hit3-1>
|
|
|
889
|
|
|
890 <div class=linkheader>
|
|
|
891 <div class=right><a href="#description3-1">Descriptions</a></div>
|
|
|
892 <a class="linkheader" href="http://example.com/example-genebank/AB209952.1/">Gene Bank</a>
|
|
|
893 </div>
|
|
|
894
|
|
|
895 <div class=title>
|
|
|
896 <p class=hittitle>AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000</p>
|
|
|
897 <p class=titleinfo>
|
|
|
898 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/AB209952.1/">gnl|BL_ORD_ID|5</a>
|
|
|
899 <span class=b>Length:</span> 2457
|
|
|
900 <span class=b>Number of Matches:</span> 1
|
|
|
901 </p>
|
|
|
902 </div>
|
|
|
903
|
|
|
904
|
|
|
905 <div class=hotspot id=hotspot3-1-1>
|
|
|
906 <p class=range>
|
|
|
907 <span class=range>Range 1: 2143 to 2162</span>
|
|
|
908 </p>
|
|
|
909
|
|
|
910 <table class=hotspotstable>
|
|
|
911 <tr>
|
|
|
912 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
913 </tr>
|
|
|
914 <tr>
|
|
|
915 <td>40.1 bits(20)</td>
|
|
|
916 <td>0.0</td>
|
|
|
917 <td>20/20(100%)</td>
|
|
|
918 <td>0/20(0%)</td>
|
|
|
919 <td>Plus/Plus</td>
|
|
|
920 </tr>
|
|
|
921 </table>
|
|
|
922
|
|
|
923 <pre class=alignmentgraphic>Query 1 CGCGCGCGGTGTCATCTATG 20
|
|
|
924 ||||||||||||||||||||
|
|
|
925 Subject 2143 CGCGCGCGGTGTCATCTATG 2162</pre>
|
|
|
926 </div>
|
|
|
927
|
|
|
928 </div>
|
|
|
929
|
|
|
930 <div class=alignment id=hit3-2>
|
|
|
931
|
|
|
932 <div class=linkheader>
|
|
|
933 <div class=right><a href="#description3-2">Descriptions</a></div>
|
|
|
934 <a class="linkheader" href="http://example.com/example-genebank/DJ437711/">Gene Bank</a>
|
|
|
935 </div>
|
|
|
936
|
|
|
937 <div class=title>
|
|
|
938 <p class=hittitle>DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000</p>
|
|
|
939 <p class=titleinfo>
|
|
|
940 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/DJ437711/">gnl|BL_ORD_ID|2</a>
|
|
|
941 <span class=b>Length:</span> 323
|
|
|
942 <span class=b>Number of Matches:</span> 1
|
|
|
943 </p>
|
|
|
944 </div>
|
|
|
945
|
|
|
946
|
|
|
947 <div class=hotspot id=hotspot3-2-1>
|
|
|
948 <p class=range>
|
|
|
949 <span class=range>Range 1: 224 to 243</span>
|
|
|
950 </p>
|
|
|
951
|
|
|
952 <table class=hotspotstable>
|
|
|
953 <tr>
|
|
|
954 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
955 </tr>
|
|
|
956 <tr>
|
|
|
957 <td>40.1 bits(20)</td>
|
|
|
958 <td>0.0</td>
|
|
|
959 <td>20/20(100%)</td>
|
|
|
960 <td>0/20(0%)</td>
|
|
|
961 <td>Plus/Plus</td>
|
|
|
962 </tr>
|
|
|
963 </table>
|
|
|
964
|
|
|
965 <pre class=alignmentgraphic>Query 1 CGCGCGCGGTGTCATCTATG 20
|
|
|
966 ||||||||||||||||||||
|
|
|
967 Subject 224 CGCGCGCGGTGTCATCTATG 243</pre>
|
|
|
968 </div>
|
|
|
969
|
|
|
970 </div>
|
|
|
971
|
|
|
972 <div class=alignment id=hit3-3>
|
|
|
973
|
|
|
974 <div class=linkheader>
|
|
|
975 <div class=right><a href="#description3-3">Descriptions</a></div>
|
|
|
976 <a class="linkheader" href="http://example.com/example-genebank/AJ308515.1/">Gene Bank</a>
|
|
|
977 </div>
|
|
|
978
|
|
|
979 <div class=title>
|
|
|
980 <p class=hittitle>AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3'UTR/plant_junction_region.|-9105899556052450000</p>
|
|
|
981 <p class=titleinfo>
|
|
|
982 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/AJ308515.1/">gnl|BL_ORD_ID|1</a>
|
|
|
983 <span class=b>Length:</span> 1045
|
|
|
984 <span class=b>Number of Matches:</span> 1
|
|
|
985 </p>
|
|
|
986 </div>
|
|
|
987
|
|
|
988
|
|
|
989 <div class=hotspot id=hotspot3-3-1>
|
|
|
990 <p class=range>
|
|
|
991 <span class=range>Range 1: 2 to 18</span>
|
|
|
992 </p>
|
|
|
993
|
|
|
994 <table class=hotspotstable>
|
|
|
995 <tr>
|
|
|
996 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
997 </tr>
|
|
|
998 <tr>
|
|
|
999 <td>34.2 bits(17)</td>
|
|
|
1000 <td>0.0</td>
|
|
|
1001 <td>17/17(100%)</td>
|
|
|
1002 <td>0/17(0%)</td>
|
|
|
1003 <td>Plus/Plus</td>
|
|
|
1004 </tr>
|
|
|
1005 </table>
|
|
|
1006
|
|
|
1007 <pre class=alignmentgraphic>Query 4 GCGCGGTGTCATCTATG 20
|
|
|
1008 |||||||||||||||||
|
|
|
1009 Subject 2 GCGCGGTGTCATCTATG 18</pre>
|
|
|
1010 </div>
|
|
|
1011
|
|
|
1012 </div>
|
|
|
1013
|
|
|
1014 </div></div>
|
|
|
1015 </section>
|
|
|
1016 </section>
|
|
|
1017
|
|
|
1018 <section class=match id=match4>
|
|
|
1019
|
|
|
1020 <h1>Nucleotide Sequence (24 letters)</h1>
|
|
|
1021
|
|
|
1022 <section class=header>
|
|
|
1023
|
|
|
1024 <table class=headerdata>
|
|
|
1025 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
1026 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
1027 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
1028 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
1029 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
1030 </table>
|
|
|
1031
|
|
|
1032 </section>
|
|
|
1033
|
|
|
1034 <section>
|
|
|
1035 <h2>No Hits</h2>
|
|
|
1036 <div class=grey>
|
|
|
1037 <table class=headerdata>
|
|
|
1038 <tr><td class=param>Message:</td><td>No hits found</td></tr>
|
|
|
1039 </table>
|
|
|
1040 </div>
|
|
|
1041 </section>
|
|
|
1042 </section>
|
|
|
1043
|
|
|
1044 <section class=match id=match5>
|
|
|
1045
|
|
|
1046 <h1>Nucleotide Sequence (20 letters)</h1>
|
|
|
1047
|
|
|
1048 <section class=header>
|
|
|
1049
|
|
|
1050 <table class=headerdata>
|
|
|
1051 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
1052 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
1053 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
1054 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
1055 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
1056 </table>
|
|
|
1057
|
|
|
1058 </section>
|
|
|
1059
|
|
|
1060 <section>
|
|
|
1061 <h2>No Hits</h2>
|
|
|
1062 <div class=grey>
|
|
|
1063 <table class=headerdata>
|
|
|
1064 <tr><td class=param>Message:</td><td>No hits found</td></tr>
|
|
|
1065 </table>
|
|
|
1066 </div>
|
|
|
1067 </section>
|
|
|
1068 </section>
|
|
|
1069
|
|
|
1070 <section class=match id=match6>
|
|
|
1071
|
|
|
1072 <h1>Nucleotide Sequence (25 letters)</h1>
|
|
|
1073
|
|
|
1074 <section class=header>
|
|
|
1075
|
|
|
1076 <table class=headerdata>
|
|
|
1077 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
1078 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
1079 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
1080 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
1081 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
1082 </table>
|
|
|
1083
|
|
|
1084 </section>
|
|
|
1085
|
|
|
1086
|
|
|
1087 <section class=graphics>
|
|
|
1088 <h2>Graphic Summary</h2>
|
|
|
1089
|
|
|
1090 <div class=grey>
|
|
|
1091 <h3 class=centered>Distribution of 7 Blast Hits on the Query Sequence</h3>
|
|
|
1092
|
|
|
1093 <div class=defline id=defline6>
|
|
|
1094 Mouse-over to show defline and scores, click to show alignments
|
|
|
1095 </div>
|
|
|
1096
|
|
|
1097 <div class=graphic>
|
|
|
1098 <h4 class=darkHeader>Color key for alignment scores</h4>
|
|
|
1099 <div class=legend><div class=graphicrow>
|
|
|
1100 <div class=graphicitem style="background-color: black"><40</div>
|
|
|
1101 <div class=graphicitem style="background-color: blue">40–50</div>
|
|
|
1102 <div class=graphicitem style="background-color: green">50–80</div>
|
|
|
1103 <div class=graphicitem style="background-color: magenta">80–200</div>
|
|
|
1104 <div class=graphicitem style="background-color: red">200≤</div>
|
|
|
1105 </div></div>
|
|
|
1106 <div style="clear: left"></div>
|
|
|
1107
|
|
|
1108 <div class=tablewrapper>
|
|
|
1109
|
|
|
1110 <div class=scale>
|
|
|
1111 <div>query:</div>
|
|
|
1112 <div class=graphicrow>
|
|
|
1113 <div>
|
|
|
1114 <div class=graphicitem style="width: 12%">
|
|
|
1115 <div>3</div>
|
|
|
1116 </div>
|
|
|
1117 <div class=graphicitem style="width: 12%">
|
|
|
1118 <div>6</div>
|
|
|
1119 </div>
|
|
|
1120 <div class=graphicitem style="width: 12%">
|
|
|
1121 <div>9</div>
|
|
|
1122 </div>
|
|
|
1123 <div class=graphicitem style="width: 12%">
|
|
|
1124 <div>12</div>
|
|
|
1125 </div>
|
|
|
1126 <div class=graphicitem style="width: 12%">
|
|
|
1127 <div>15</div>
|
|
|
1128 </div>
|
|
|
1129 <div class=graphicitem style="width: 12%">
|
|
|
1130 <div>18</div>
|
|
|
1131 </div>
|
|
|
1132 <div class=graphicitem style="width: 12%">
|
|
|
1133 <div>21</div>
|
|
|
1134 </div>
|
|
|
1135 <div class=graphicitem style="width: 12%">
|
|
|
1136 <div>24</div>
|
|
|
1137 </div>
|
|
|
1138 <div class=graphicitem style="width: 4%">
|
|
|
1139 <div>25</div>
|
|
|
1140 </div>
|
|
|
1141 </div>
|
|
|
1142 </div>
|
|
|
1143 <div style="clear: left"></div>
|
|
|
1144 </div>
|
|
|
1145
|
|
|
1146 <a class=matchresult
|
|
|
1147 href="#hit6-1"
|
|
|
1148 onmouseover='document.getElementById("defline6").innerHTML="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000"'
|
|
|
1149 onmouseout='document.getElementById("defline6").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
1150 title="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000">
|
|
|
1151 <div class="matchrow graphicrow">
|
|
|
1152 <div class="matchitem graphicitem"
|
|
|
1153 style="background-color: black; width: 88%"></div>
|
|
|
1154 <div class="matchitem graphicitem"
|
|
|
1155 style="background-color: transparent; width: 12%"></div>
|
|
|
1156 </div>
|
|
|
1157 </a>
|
|
|
1158
|
|
|
1159 <a class=matchresult
|
|
|
1160 href="#hit6-2"
|
|
|
1161 onmouseover='document.getElementById("defline6").innerHTML="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000"'
|
|
|
1162 onmouseout='document.getElementById("defline6").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
1163 title="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000">
|
|
|
1164 <div class="matchrow graphicrow">
|
|
|
1165 <div class="matchitem graphicitem"
|
|
|
1166 style="background-color: black; width: 88%"></div>
|
|
|
1167 <div class="matchitem graphicitem"
|
|
|
1168 style="background-color: transparent; width: 12%"></div>
|
|
|
1169 </div>
|
|
|
1170 </a>
|
|
|
1171
|
|
|
1172 </div>
|
|
|
1173 </div>
|
|
|
1174 </div>
|
|
|
1175 </section>
|
|
|
1176
|
|
|
1177
|
|
|
1178
|
|
|
1179 <section class=descriptions>
|
|
|
1180 <h2>Descriptions</h2>
|
|
|
1181
|
|
|
1182 <div class=grey><div class=white>
|
|
|
1183 <h4 class=darkHeader>Sequences producing significant alignments:</h4>
|
|
|
1184
|
|
|
1185 <table class=descriptiontable>
|
|
|
1186 <col/><col/><col/><col/><col/><col/><col/>
|
|
|
1187 <tr>
|
|
|
1188 <th>Description</th>
|
|
|
1189 <th>Max score</th>
|
|
|
1190 <th>Total score</th>
|
|
|
1191 <th>Query cover</th>
|
|
|
1192 <th>E value</th>
|
|
|
1193 <th>Ident</th>
|
|
|
1194 <th>Accession</th>
|
|
|
1195 </tr>
|
|
|
1196 <tr>
|
|
|
1197 <td><div><a href="#hit6-1"
|
|
|
1198 title="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000"
|
|
|
1199 id="description6-1">
|
|
|
1200 EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000
|
|
|
1201 </a></div></td>
|
|
|
1202 <td>36.2</td>
|
|
|
1203 <td>36.2</td>
|
|
|
1204 <td>88%</td>
|
|
|
1205 <td>3.148e-06</td>
|
|
|
1206 <td>95%</td>
|
|
|
1207 <td><a href="http://example.com/example-genebank/EUG/">7</a></td>
|
|
|
1208 </tr>
|
|
|
1209 <tr>
|
|
|
1210 <td><div><a href="#hit6-2"
|
|
|
1211 title="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000"
|
|
|
1212 id="description6-2">
|
|
|
1213 AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000
|
|
|
1214 </a></div></td>
|
|
|
1215 <td>36.2</td>
|
|
|
1216 <td>36.2</td>
|
|
|
1217 <td>88%</td>
|
|
|
1218 <td>3.148e-06</td>
|
|
|
1219 <td>95%</td>
|
|
|
1220 <td><a href="http://example.com/example-genebank/AY326434/">6</a></td>
|
|
|
1221 </tr>
|
|
|
1222 </table>
|
|
|
1223
|
|
|
1224 </div></div>
|
|
|
1225 </section>
|
|
|
1226
|
|
|
1227
|
|
|
1228
|
|
|
1229 <section class=alignments>
|
|
|
1230 <h2>Alignments</h2>
|
|
|
1231
|
|
|
1232 <div class=grey><div class=white>
|
|
|
1233 <div class=alignment id=hit6-1>
|
|
|
1234
|
|
|
1235 <div class=linkheader>
|
|
|
1236 <div class=right><a href="#description6-1">Descriptions</a></div>
|
|
|
1237 <a class="linkheader" href="http://example.com/example-genebank/EUG/">Gene Bank</a>
|
|
|
1238 </div>
|
|
|
1239
|
|
|
1240 <div class=title>
|
|
|
1241 <p class=hittitle>EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000</p>
|
|
|
1242 <p class=titleinfo>
|
|
|
1243 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/EUG/">gnl|BL_ORD_ID|7</a>
|
|
|
1244 <span class=b>Length:</span> 4983
|
|
|
1245 <span class=b>Number of Matches:</span> 1
|
|
|
1246 </p>
|
|
|
1247 </div>
|
|
|
1248
|
|
|
1249
|
|
|
1250 <div class=hotspot id=hotspot6-1-1>
|
|
|
1251 <p class=range>
|
|
|
1252 <span class=range>Range 1: 2344 to 2365</span>
|
|
|
1253 </p>
|
|
|
1254
|
|
|
1255 <table class=hotspotstable>
|
|
|
1256 <tr>
|
|
|
1257 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
1258 </tr>
|
|
|
1259 <tr>
|
|
|
1260 <td>36.2 bits(18)</td>
|
|
|
1261 <td>0.0</td>
|
|
|
1262 <td>21/22(95%)</td>
|
|
|
1263 <td>0/22(0%)</td>
|
|
|
1264 <td>Plus/Plus</td>
|
|
|
1265 </tr>
|
|
|
1266 </table>
|
|
|
1267
|
|
|
1268 <pre class=alignmentgraphic>Query 1 ACATGAACAGCGCCTTGACCAC 22
|
|
|
1269 |||||||||||||| |||||||
|
|
|
1270 Subject 2344 ACATGAACAGCGCCCTGACCAC 2365</pre>
|
|
|
1271 </div>
|
|
|
1272
|
|
|
1273 </div>
|
|
|
1274
|
|
|
1275 <div class=alignment id=hit6-2>
|
|
|
1276
|
|
|
1277 <div class=linkheader>
|
|
|
1278 <div class=right><a href="#description6-2">Descriptions</a></div>
|
|
|
1279 <a class="linkheader" href="http://example.com/example-genebank/AY326434/">Gene Bank</a>
|
|
|
1280 </div>
|
|
|
1281
|
|
|
1282 <div class=title>
|
|
|
1283 <p class=hittitle>AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000</p>
|
|
|
1284 <p class=titleinfo>
|
|
|
1285 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/AY326434/">gnl|BL_ORD_ID|6</a>
|
|
|
1286 <span class=b>Length:</span> 4180
|
|
|
1287 <span class=b>Number of Matches:</span> 1
|
|
|
1288 </p>
|
|
|
1289 </div>
|
|
|
1290
|
|
|
1291
|
|
|
1292 <div class=hotspot id=hotspot6-2-1>
|
|
|
1293 <p class=range>
|
|
|
1294 <span class=range>Range 1: 1541 to 1562</span>
|
|
|
1295 </p>
|
|
|
1296
|
|
|
1297 <table class=hotspotstable>
|
|
|
1298 <tr>
|
|
|
1299 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
1300 </tr>
|
|
|
1301 <tr>
|
|
|
1302 <td>36.2 bits(18)</td>
|
|
|
1303 <td>0.0</td>
|
|
|
1304 <td>21/22(95%)</td>
|
|
|
1305 <td>0/22(0%)</td>
|
|
|
1306 <td>Plus/Plus</td>
|
|
|
1307 </tr>
|
|
|
1308 </table>
|
|
|
1309
|
|
|
1310 <pre class=alignmentgraphic>Query 1 ACATGAACAGCGCCTTGACCAC 22
|
|
|
1311 |||||||||||||| |||||||
|
|
|
1312 Subject 1541 ACATGAACAGCGCCCTGACCAC 1562</pre>
|
|
|
1313 </div>
|
|
|
1314
|
|
|
1315 </div>
|
|
|
1316
|
|
|
1317 </div></div>
|
|
|
1318 </section>
|
|
|
1319 </section>
|
|
|
1320
|
|
|
1321 <section class=match id=match7>
|
|
|
1322
|
|
|
1323 <h1>Nucleotide Sequence (74 letters)</h1>
|
|
|
1324
|
|
|
1325 <section class=header>
|
|
|
1326
|
|
|
1327 <table class=headerdata>
|
|
|
1328 <tr><td class=param>Query ID:</td><td>Query_1</td></tr>
|
|
|
1329 <tr><td class=param>Query definition:</td><td>Forward_Primer|NOST-Spec_(Genetic_ID)</td></tr>
|
|
|
1330 <tr><td class=param>Query length:</td><td>20</td></tr>
|
|
|
1331 <tr><td class=param>Program:</td><td>BLASTN 2.2.29+</td></tr>
|
|
|
1332 <tr><td class=param>Database:</td><td>/opt/galaxy/blastdbs/EUginius_plasmid_insert</td></tr>
|
|
|
1333 </table>
|
|
|
1334
|
|
|
1335 </section>
|
|
|
1336
|
|
|
1337
|
|
|
1338 <section class=graphics>
|
|
|
1339 <h2>Graphic Summary</h2>
|
|
|
1340
|
|
|
1341 <div class=grey>
|
|
|
1342 <h3 class=centered>Distribution of 7 Blast Hits on the Query Sequence</h3>
|
|
|
1343
|
|
|
1344 <div class=defline id=defline7>
|
|
|
1345 Mouse-over to show defline and scores, click to show alignments
|
|
|
1346 </div>
|
|
|
1347
|
|
|
1348 <div class=graphic>
|
|
|
1349 <h4 class=darkHeader>Color key for alignment scores</h4>
|
|
|
1350 <div class=legend><div class=graphicrow>
|
|
|
1351 <div class=graphicitem style="background-color: black"><40</div>
|
|
|
1352 <div class=graphicitem style="background-color: blue">40–50</div>
|
|
|
1353 <div class=graphicitem style="background-color: green">50–80</div>
|
|
|
1354 <div class=graphicitem style="background-color: magenta">80–200</div>
|
|
|
1355 <div class=graphicitem style="background-color: red">200≤</div>
|
|
|
1356 </div></div>
|
|
|
1357 <div style="clear: left"></div>
|
|
|
1358
|
|
|
1359 <div class=tablewrapper>
|
|
|
1360
|
|
|
1361 <div class=scale>
|
|
|
1362 <div>query:</div>
|
|
|
1363 <div class=graphicrow>
|
|
|
1364 <div>
|
|
|
1365 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1366 <div>8</div>
|
|
|
1367 </div>
|
|
|
1368 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1369 <div>16</div>
|
|
|
1370 </div>
|
|
|
1371 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1372 <div>24</div>
|
|
|
1373 </div>
|
|
|
1374 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1375 <div>32</div>
|
|
|
1376 </div>
|
|
|
1377 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1378 <div>40</div>
|
|
|
1379 </div>
|
|
|
1380 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1381 <div>48</div>
|
|
|
1382 </div>
|
|
|
1383 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1384 <div>56</div>
|
|
|
1385 </div>
|
|
|
1386 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1387 <div>64</div>
|
|
|
1388 </div>
|
|
|
1389 <div class=graphicitem style="width: 10.8108108108%">
|
|
|
1390 <div>72</div>
|
|
|
1391 </div>
|
|
|
1392 <div class=graphicitem style="width: 2.7027027027%">
|
|
|
1393 <div> </div>
|
|
|
1394 <div class=lastlabel>74</div>
|
|
|
1395 </div>
|
|
|
1396 </div>
|
|
|
1397 </div>
|
|
|
1398 <div style="clear: left"></div>
|
|
|
1399 </div>
|
|
|
1400
|
|
|
1401 <a class=matchresult
|
|
|
1402 href="#hit7-1"
|
|
|
1403 onmouseover='document.getElementById("defline7").innerHTML="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000"'
|
|
|
1404 onmouseout='document.getElementById("defline7").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
1405 title="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000">
|
|
|
1406 <div class="matchrow graphicrow">
|
|
|
1407 <div class="matchitem graphicitem"
|
|
|
1408 style="background-color: green; width: 100%"></div>
|
|
|
1409 </div>
|
|
|
1410 </a>
|
|
|
1411
|
|
|
1412 <a class=matchresult
|
|
|
1413 href="#hit7-2"
|
|
|
1414 onmouseover='document.getElementById("defline7").innerHTML="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000"'
|
|
|
1415 onmouseout='document.getElementById("defline7").innerHTML="Mouse-over to show defline and scores, click to show alignments"'
|
|
|
1416 title="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000">
|
|
|
1417 <div class="matchrow graphicrow">
|
|
|
1418 <div class="matchitem graphicitem"
|
|
|
1419 style="background-color: green; width: 100%"></div>
|
|
|
1420 </div>
|
|
|
1421 </a>
|
|
|
1422
|
|
|
1423 </div>
|
|
|
1424 </div>
|
|
|
1425 </div>
|
|
|
1426 </section>
|
|
|
1427
|
|
|
1428
|
|
|
1429
|
|
|
1430 <section class=descriptions>
|
|
|
1431 <h2>Descriptions</h2>
|
|
|
1432
|
|
|
1433 <div class=grey><div class=white>
|
|
|
1434 <h4 class=darkHeader>Sequences producing significant alignments:</h4>
|
|
|
1435
|
|
|
1436 <table class=descriptiontable>
|
|
|
1437 <col/><col/><col/><col/><col/><col/><col/>
|
|
|
1438 <tr>
|
|
|
1439 <th>Description</th>
|
|
|
1440 <th>Max score</th>
|
|
|
1441 <th>Total score</th>
|
|
|
1442 <th>Query cover</th>
|
|
|
1443 <th>E value</th>
|
|
|
1444 <th>Ident</th>
|
|
|
1445 <th>Accession</th>
|
|
|
1446 </tr>
|
|
|
1447 <tr>
|
|
|
1448 <td><div><a href="#hit7-1"
|
|
|
1449 title="EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000"
|
|
|
1450 id="description7-1">
|
|
|
1451 EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000
|
|
|
1452 </a></div></td>
|
|
|
1453 <td>67.9</td>
|
|
|
1454 <td>67.9</td>
|
|
|
1455 <td>100%</td>
|
|
|
1456 <td>3.564e-15</td>
|
|
|
1457 <td>86%</td>
|
|
|
1458 <td><a href="http://example.com/example-genebank/EUG/">7</a></td>
|
|
|
1459 </tr>
|
|
|
1460 <tr>
|
|
|
1461 <td><div><a href="#hit7-2"
|
|
|
1462 title="AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000"
|
|
|
1463 id="description7-2">
|
|
|
1464 AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000
|
|
|
1465 </a></div></td>
|
|
|
1466 <td>67.9</td>
|
|
|
1467 <td>67.9</td>
|
|
|
1468 <td>100%</td>
|
|
|
1469 <td>3.564e-15</td>
|
|
|
1470 <td>86%</td>
|
|
|
1471 <td><a href="http://example.com/example-genebank/AY326434/">6</a></td>
|
|
|
1472 </tr>
|
|
|
1473 </table>
|
|
|
1474
|
|
|
1475 </div></div>
|
|
|
1476 </section>
|
|
|
1477
|
|
|
1478
|
|
|
1479
|
|
|
1480 <section class=alignments>
|
|
|
1481 <h2>Alignments</h2>
|
|
|
1482
|
|
|
1483 <div class=grey><div class=white>
|
|
|
1484 <div class=alignment id=hit7-1>
|
|
|
1485
|
|
|
1486 <div class=linkheader>
|
|
|
1487 <div class=right><a href="#description7-1">Descriptions</a></div>
|
|
|
1488 <a class="linkheader" href="http://example.com/example-genebank/EUG/">Gene Bank</a>
|
|
|
1489 </div>
|
|
|
1490
|
|
|
1491 <div class=title>
|
|
|
1492 <p class=hittitle>EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000</p>
|
|
|
1493 <p class=titleinfo>
|
|
|
1494 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/EUG/">gnl|BL_ORD_ID|7</a>
|
|
|
1495 <span class=b>Length:</span> 4983
|
|
|
1496 <span class=b>Number of Matches:</span> 1
|
|
|
1497 </p>
|
|
|
1498 </div>
|
|
|
1499
|
|
|
1500
|
|
|
1501 <div class=hotspot id=hotspot7-1-1>
|
|
|
1502 <p class=range>
|
|
|
1503 <span class=range>Range 1: 2319 to 2392</span>
|
|
|
1504 </p>
|
|
|
1505
|
|
|
1506 <table class=hotspotstable>
|
|
|
1507 <tr>
|
|
|
1508 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
1509 </tr>
|
|
|
1510 <tr>
|
|
|
1511 <td>67.9 bits(34)</td>
|
|
|
1512 <td>0.0</td>
|
|
|
1513 <td>64/74(86%)</td>
|
|
|
1514 <td>0/74(0%)</td>
|
|
|
1515 <td>Plus/Plus</td>
|
|
|
1516 </tr>
|
|
|
1517 </table>
|
|
|
1518
|
|
|
1519 <pre class=alignmentgraphic>Query 1 GAGGAAATGCGTATTCAATTCAACGACATGAACAGCGCCTTGACCACAGCTATCCCATTG 60
|
|
|
1520 ||||| ||||| || || ||||||||||||||||||||| ||||||| || |||||| |
|
|
|
1521 Subject 2319 GAGGAGATGCGCATCCAGTTCAACGACATGAACAGCGCCCTGACCACCGCCATCCCACTC 2378</pre>
|
|
|
1522 <pre class=alignmentgraphic>Query 61 TTCGCAGTCCAGAA 74
|
|
|
1523 ||||| ||||||||
|
|
|
1524 Subject 2379 TTCGCCGTCCAGAA 2392</pre>
|
|
|
1525 </div>
|
|
|
1526
|
|
|
1527 </div>
|
|
|
1528
|
|
|
1529 <div class=alignment id=hit7-2>
|
|
|
1530
|
|
|
1531 <div class=linkheader>
|
|
|
1532 <div class=right><a href="#description7-2">Descriptions</a></div>
|
|
|
1533 <a class="linkheader" href="http://example.com/example-genebank/AY326434/">Gene Bank</a>
|
|
|
1534 </div>
|
|
|
1535
|
|
|
1536 <div class=title>
|
|
|
1537 <p class=hittitle>AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000</p>
|
|
|
1538 <p class=titleinfo>
|
|
|
1539 <span class=b>Sequence ID:</span> <a href="http://example.com/example-genebank/AY326434/">gnl|BL_ORD_ID|6</a>
|
|
|
1540 <span class=b>Length:</span> 4180
|
|
|
1541 <span class=b>Number of Matches:</span> 1
|
|
|
1542 </p>
|
|
|
1543 </div>
|
|
|
1544
|
|
|
1545
|
|
|
1546 <div class=hotspot id=hotspot7-2-1>
|
|
|
1547 <p class=range>
|
|
|
1548 <span class=range>Range 1: 1589 to 1516</span>
|
|
|
1549 </p>
|
|
|
1550
|
|
|
1551 <table class=hotspotstable>
|
|
|
1552 <tr>
|
|
|
1553 <th>Score</th><th>Expect</th><th>Identities</th><th>Gaps</th><th>Strand</th>
|
|
|
1554 </tr>
|
|
|
1555 <tr>
|
|
|
1556 <td>67.9 bits(34)</td>
|
|
|
1557 <td>0.0</td>
|
|
|
1558 <td>64/74(86%)</td>
|
|
|
1559 <td>0/74(0%)</td>
|
|
|
1560 <td>Plus/Minus</td>
|
|
|
1561 </tr>
|
|
|
1562 </table>
|
|
|
1563
|
|
|
1564 <pre class=alignmentgraphic>Query 1 GAGGAAATGCGTATTCAATTCAACGACATGAACAGCGCCTTGACCACAGCTATCCCATTG 60
|
|
|
1565 ||||| ||||| || || ||||||||||||||||||||| ||||||| || |||||| |
|
|
|
1566 Subject 1589 GAGGAGATGCGCATCCAGTTCAACGACATGAACAGCGCCCTGACCACCGCCATCCCACTC 1530</pre>
|
|
|
1567 <pre class=alignmentgraphic>Query 61 TTCGCAGTCCAGAA 74
|
|
|
1568 ||||| ||||||||
|
|
|
1569 Subject 1529 TTCGCCGTCCAGAA 1516</pre>
|
|
|
1570 </div>
|
|
|
1571
|
|
|
1572 </div>
|
|
|
1573
|
|
|
1574 </div></div>
|
|
|
1575 </section>
|
|
|
1576 </section>
|
|
|
1577
|
|
|
1578 </div>
|
|
|
1579
|
|
|
1580 <footer>
|
|
|
1581 This page was generated by <a href="https://github.com/thehyve/blast2html/">blast2html</a>.
|
|
|
1582 </footer>
|
|
|
1583 </body>
|
|
|
1584 </html>
|
|
|
1585
|
