annotate visualise.py @ 11:7660519f2dc9

proper layout for alignments, added some links
author Jan Kanis <jan.code@jankanis.nl>
date Mon, 12 May 2014 13:55:04 +0200
parents 2fbdf2eb27b4
children a459c754cdb5
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1 #!/usr/bin/env python3
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2
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3 # Copyright The Hyve B.V. 2014
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4 # License: GPL version 3 or higher
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5
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6 import sys
7
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7 import math
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8 import warnings
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9 from itertools import repeat
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10 from lxml import objectify
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11 import jinja2
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12
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13
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14 blast = objectify.parse('blast xml example1.xml').getroot()
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15 loader = jinja2.FileSystemLoader(searchpath='.')
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16 environment = jinja2.Environment(loader=loader, lstrip_blocks=True, trim_blocks=True, autoescape=True)
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17
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18 def filter(func_or_name):
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19 "Decorator to register a function as filter in the current jinja environment"
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20 if isinstance(func_or_name, str):
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21 def inner(func):
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22 environment.filters[func_or_name] = func
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23 return func
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24 return inner
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25 else:
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26 environment.filters[func_or_name.__name__] = func_or_name
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27 return func_or_name
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28
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29
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30 def color_idx(length):
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31 if length < 40:
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32 return 0
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33 elif length < 50:
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34 return 1
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35 elif length < 80:
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36 return 2
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37 elif length < 200:
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38 return 3
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39 return 4
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40
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41 colors = ['black', 'blue', 'green', 'magenta', 'red']
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42
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43 environment.filters['color'] = lambda length: match_colors[color_idx(length)]
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44
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45 @filter
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46 def fmt(val, fmt):
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47 return format(float(val), fmt)
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48
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49 @filter
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50 def firsttitle(hit):
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51 return hit.Hit_def.text.split('>')[0]
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52
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53 @filter
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54 def othertitles(hit):
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55 """Split a hit.Hit_def that contains multiple titles up, splitting out the hit ids from the titles."""
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56 id_titles = hit.Hit_def.text.split('>')
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57
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58 titles = []
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59 for t in id_titles[1:]:
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60 fullid, title = t.split(' ', 1)
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61 id = fullid.split('|', 2)[2]
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62 titles.append(dict(id = id,
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63 fullid = fullid,
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64 title = title))
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65 return titles
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66
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67 @filter
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68 def hitid(hit):
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69 return hit.Hit_id.text.split('|', 2)[1]
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70
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71 @filter
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72 def seqid(hit):
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73 return hit.Hit_id.text.split('|', 2)[2]
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74
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75 @filter
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76 def alignment_pre(hsp):
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77 return (
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78 "Query {:>7s} {} {}\n".format(hsp['Hsp_query-from'], hsp.Hsp_qseq, hsp['Hsp_query-to']) +
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79 " {:7s} {}\n".format('', hsp.Hsp_midline) +
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80 "Subject{:>7s} {} {}".format(hsp['Hsp_hit-from'], hsp.Hsp_hseq, hsp['Hsp_hit-to'])
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81 )
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82
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83 @filter('len')
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84 def hsplen(node):
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85 return int(node['Hsp_align-len'])
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86
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87 @filter
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88 def asframe(frame):
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89 if frame == 1:
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90 return 'Plus'
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91 elif frame == -1:
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92 return 'Minus'
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93 raise Exception("frame should be either +1 or -1")
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94
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95
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96 query_length = int(blast["BlastOutput_query-len"])
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97
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98 hits = blast.BlastOutput_iterations.Iteration.Iteration_hits.Hit
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99 # sort hits by longest hotspot first
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100 ordered_hits = sorted(hits,
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101 key=lambda h: max(hsplen(hsp) for hsp in h.Hit_hsps.Hsp),
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102 reverse=True)
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103
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104 def match_colors():
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105 """
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106 An iterator that yields lists of length-color pairs.
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107 """
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108
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109 percent_multiplier = 100 / query_length
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110
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111 for hit in hits:
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112 # sort hotspots from short to long, so we can overwrite index colors of
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113 # short matches with those of long ones.
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114 hotspots = sorted(hit.Hit_hsps.Hsp, key=lambda hsp: hsplen(hsp))
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115 table = bytearray([255]) * query_length
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116 for hsp in hotspots:
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117 frm = hsp['Hsp_query-from'] - 1
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118 to = int(hsp['Hsp_query-to'])
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119 table[frm:to] = repeat(color_idx(hsplen(hsp)), to - frm)
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120
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121 matches = []
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122 last = table[0]
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123 count = 0
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124 for i in range(query_length):
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125 if table[i] == last:
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126 count += 1
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127 continue
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128 matches.append((count * percent_multiplier, colors[last] if last != 255 else 'none'))
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129 last = table[i]
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130 count = 1
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131 matches.append((count * percent_multiplier, colors[last] if last != 255 else 'none'))
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132
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133 yield dict(colors=matches, link="#hit"+hit.Hit_num.text, defline=firsttitle(hit))
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134
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135
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136 def queryscale():
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137 max_labels = 10
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138 skip = math.ceil(query_length / max_labels)
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139 percent_multiplier = 100 / query_length
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140 for i in range(1, query_length+1):
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141 if i % skip == 0:
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142 yield dict(label = i, width = skip * percent_multiplier)
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143 if query_length % skip != 0:
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144 yield dict(label = query_length, width = (query_length % skip) * percent_multiplier)
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145
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146
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147 def hit_info():
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148
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149 for hit in ordered_hits:
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150 hsps = hit.Hit_hsps.Hsp
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151
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152 cover = [False] * query_length
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153 for hsp in hsps:
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154 cover[hsp['Hsp_query-from']-1 : int(hsp['Hsp_query-to'])] = repeat(True, hsplen(hsp))
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155 cover_count = cover.count(True)
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156
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157 def hsp_val(path):
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158 return (hsp[path] for hsp in hsps)
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159
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160 yield dict(hit = hit,
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161 title = firsttitle(hit),
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162 link_id = hit.Hit_num,
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163 maxscore = "{:.1f}".format(float(max(hsp_val('Hsp_bit-score')))),
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164 totalscore = "{:.1f}".format(float(sum(hsp_val('Hsp_bit-score')))),
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165 cover = "{:.0%}".format(cover_count / query_length),
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166 e_value = "{:.4g}".format(float(min(hsp_val('Hsp_evalue')))),
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167 # FIXME: is this the correct formula vv?
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168 ident = "{:.0%}".format(float(min(hsp.Hsp_identity / hsplen(hsp) for hsp in hsps))),
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169 accession = hit.Hit_accession)
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170
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171
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172 def main():
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173 template = environment.get_template('visualise.html.jinja')
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174
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175 params = (('Query ID', blast["BlastOutput_query-ID"]),
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176 ('Query definition', blast["BlastOutput_query-def"]),
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177 ('Query length', blast["BlastOutput_query-len"]),
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178 ('Program', blast.BlastOutput_version),
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179 ('Database', blast.BlastOutput_db),
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180 )
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181
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182 if len(blast.BlastOutput_iterations.Iteration) > 1:
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183 warnings.warn("Multiple 'Iteration' elements found, showing only the first")
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184
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185 sys.stdout.write(template.render(blast=blast,
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186 length=query_length,
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187 hits=blast.BlastOutput_iterations.Iteration.Iteration_hits.Hit,
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188 colors=colors,
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189 match_colors=match_colors(),
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190 queryscale=queryscale(),
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191 hit_info=hit_info(),
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192 params=params))
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193
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194 main()
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195
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196 # http://www.ncbi.nlm.nih.gov/nucleotide/557804451?report=genbank&log$=nuclalign&blast_rank=1&RID=PHWP1JNZ014
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197 # http://www.ncbi.nlm.nih.gov/nuccore/557804451?report=graph&rid=PHWP1JNZ014[557804451]&tracks=[key:sequence_track,name:Sequence,display_name:Sequence,id:STD1,category:Sequence,annots:Sequence,ShowLabel:true][key:gene_model_track,CDSProductFeats:false][key:alignment_track,name:other%20alignments,annots:NG%20Alignments%7CRefseq%20Alignments%7CGnomon%20Alignments%7CUnnamed,shown:false]&v=752:2685&appname=ncbiblast&link_loc=fromSubj
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198
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199 # http://www.ncbi.nlm.nih.gov/nucleotide/557804451?report=genbank&log$=nucltop&blast_rank=1&RID=PHWP1JNZ014