Nucleotide Blast results
| Program: | BLASTN 2.2.29+ | 
| Database: | /opt/galaxy/blastdbs/EUginius_plasmid_insert | 
Queries
Nucleotide Sequence (20 letters)
| Query number: | 1 | 
| Query ID: | Query_1 | 
| Definition line: | Forward_Primer|NOST-Spec_(Genetic_ID) | 
| Length: | 20 | 
Graphic Summary
Descriptions
Alignments
AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000
Sequence ID: gnl|BL_ORD_ID|5 Length: 2457 Number of Matches: 1
Range 1: 2119 to 2138
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 40.14 bits (20) | 1.51296e-07 | 20/20 (100%) | 0/20 (0%) | Plus/Plus | 
Query        1  AGCGCGCAAACTAGGATAAA  20
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Subject   2119  AGCGCGCAAACTAGGATAAA  2138
                DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000
Sequence ID: gnl|BL_ORD_ID|2 Length: 323 Number of Matches: 1
Range 1: 200 to 219
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 40.14 bits (20) | 1.51296e-07 | 20/20 (100%) | 0/20 (0%) | Plus/Plus | 
Query        1  AGCGCGCAAACTAGGATAAA  20
                ||||||||||||||||||||
Subject    200  AGCGCGCAAACTAGGATAAA  219
                Nucleotide Sequence (20 letters)
| Query number: | 2 | 
| Query ID: | Query_2 | 
| Definition line: | Reverse_Primer|NOST-Spec_(Genetic_ID) | 
| Length: | 20 | 
No Hits
| Message: | No hits found | 
Nucleotide Sequence (20 letters)
| Query number: | 3 | 
| Query ID: | Query_3 | 
| Definition line: | Probe|NOST-Spec_(Genetic_ID) | 
| Length: | 20 | 
Graphic Summary
Descriptions
Alignments
AB209952.1|GenBank|insert_GTS-40-3-2|Glycine_max_transgenic_cp4epsps_gene_for_5-enol-pyruvylshikimate-3-phospate_synthase_class_2_precursor,_complete_cds|-9105899556052450000
Sequence ID: gnl|BL_ORD_ID|5 Length: 2457 Number of Matches: 1
Range 1: 2143 to 2162
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 40.14 bits (20) | 1.51296e-07 | 20/20 (100%) | 0/20 (0%) | Plus/Plus | 
Query        1  CGCGCGCGGTGTCATCTATG  20
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Subject   2143  CGCGCGCGGTGTCATCTATG  2162
                DJ437711|GenBank|insert_MIR604|Corn_Event_MIR604,_Left_Border_region|-5751164067366620000
Sequence ID: gnl|BL_ORD_ID|2 Length: 323 Number of Matches: 1
Range 1: 224 to 243
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 40.14 bits (20) | 1.51296e-07 | 20/20 (100%) | 0/20 (0%) | Plus/Plus | 
Query        1  CGCGCGCGGTGTCATCTATG  20
                ||||||||||||||||||||
Subject    224  CGCGCGCGGTGTCATCTATG  243
                AJ308515.1|GenBank|insert_GTS-40-3-2|Synthetic_construct_for_NOS_3'UTR/plant_junction_region.|-9105899556052450000
Sequence ID: gnl|BL_ORD_ID|1 Length: 1045 Number of Matches: 1
Range 1: 2 to 18
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 34.1929 bits (17) | 9.33411e-06 | 17/17 (100%) | 0/17 (0%) | Plus/Plus | 
Query        4  GCGCGGTGTCATCTATG  20
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Subject      2  GCGCGGTGTCATCTATG  18
                Nucleotide Sequence (24 letters)
| Query number: | 4 | 
| Query ID: | Query_4 | 
| Definition line: | Forward_Primer|QL-ELE-Bt-F1(mod)/Bt-R | 
| Length: | 24 | 
No Hits
| Message: | No hits found | 
Nucleotide Sequence (20 letters)
| Query number: | 5 | 
| Query ID: | Query_5 | 
| Definition line: | Reverse_Primer|QL-ELE-Bt-F1(mod)/Bt-R | 
| Length: | 20 | 
No Hits
| Message: | No hits found | 
Nucleotide Sequence (25 letters)
| Query number: | 6 | 
| Query ID: | Query_6 | 
| Definition line: | Probe|QL-ELE-Bt-F1(mod)/Bt-R | 
| Length: | 25 | 
Graphic Summary
Descriptions
Alignments
EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000
Sequence ID: gnl|BL_ORD_ID|7 Length: 4983 Number of Matches: 1
Range 1: 2344 to 2365
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 36.1753 bits (18) | 3.14801e-06 | 21/22 (95%) | 0/22 (0%) | Plus/Plus | 
Query        1  ACATGAACAGCGCCTTGACCAC  22
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Subject   2344  ACATGAACAGCGCCCTGACCAC  2365
                AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000
Sequence ID: gnl|BL_ORD_ID|6 Length: 4180 Number of Matches: 1
Range 1: 1541 to 1562
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 36.1753 bits (18) | 3.14801e-06 | 21/22 (95%) | 0/22 (0%) | Plus/Plus | 
Query        1  ACATGAACAGCGCCTTGACCAC  22
                |||||||||||||| |||||||
Subject   1541  ACATGAACAGCGCCCTGACCAC  1562
                Nucleotide Sequence (74 letters)
| Query number: | 7 | 
| Query ID: | Query_7 | 
| Definition line: | Amplicon|QL-ELE-Bt-F1(mod)/Bt-R | 
| Length: | 74 | 
Graphic Summary
Descriptions
Alignments
EUG|RIKILT|plasmid_pV-ZMBK07|plasmid_pV-ZMBK07|-2635190737607180000
Sequence ID: gnl|BL_ORD_ID|7 Length: 4983 Number of Matches: 1
Range 1: 2319 to 2392
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 67.8929 bits (34) | 3.56369e-15 | 64/74 (86%) | 0/74 (0%) | Plus/Plus | 
Query        1  GAGGAAATGCGTATTCAATTCAACGACATGAACAGCGCCTTGACCACAGCTATCCCATTG  60
                ||||| ||||| || || ||||||||||||||||||||| ||||||| || |||||| | 
Subject   2319  GAGGAGATGCGCATCCAGTTCAACGACATGAACAGCGCCCTGACCACCGCCATCCCACTC  2378
                  Query       61  TTCGCAGTCCAGAA  74
                ||||| ||||||||
Subject   2379  TTCGCCGTCCAGAA  2392
                AY326434|GenBank|insert_MON810|Synthetic_construct_truncated_CRYIA(b)_(cryIA(b))_gene,_partial_CDS|-2635190737607180000
Sequence ID: gnl|BL_ORD_ID|6 Length: 4180 Number of Matches: 1
Range 1: 1589 to 1516
| Score | Expect | Identities | Gaps | Strand | 
|---|---|---|---|---|
| 67.8929 bits (34) | 3.56369e-15 | 64/74 (86%) | 0/74 (0%) | Plus/Minus | 
Query        1  GAGGAAATGCGTATTCAATTCAACGACATGAACAGCGCCTTGACCACAGCTATCCCATTG  60
                ||||| ||||| || || ||||||||||||||||||||| ||||||| || |||||| | 
Subject   1589  GAGGAGATGCGCATCCAGTTCAACGACATGAACAGCGCCCTGACCACCGCCATCCCACTC  1530
                  Query       61  TTCGCAGTCCAGAA  74
                ||||| ||||||||
Subject   1529  TTCGCCGTCCAGAA  1516