|
6
|
1 #I should make an SOP for this. Problems we encountered: no x in the xY motif, and the kilodemon
|
|
|
2 #the output files have both Y and xY, they shouldn't why is that happening? make it not happen
|
|
|
3 #make sure that accession numbers stay locked to each motif, somehow
|
|
|
4 #output should look just like the KALIP input
|
|
|
5
|
|
|
6 #ff you want ONLY FULL MOTIFS, put "YES" here, please use all caps
|
|
|
7 FullMotifsOnly_questionmark<-"NO"
|
|
|
8 #If you want ONLY TRUNCATED MOTIFS, put "YES" here, please use all caps
|
|
|
9 TruncatedMotifsOnly_questionmark<-"NO"
|
|
|
10 #if you want to find the overlap, put a "YES" here (all caps), if you want to find the non-overlap, put "NO" (all caps)
|
|
|
11 Are_You_Looking_For_Commonality<-"NO"
|
|
|
12
|
|
|
13
|
|
|
14 #put the names of your input files here
|
|
|
15 FirstSubstrateSet<- read.csv("S1.csv", stringsAsFactors=FALSE)
|
|
|
16 Firstsubbackfreq<- read.csv("SBF1.csv", header=FALSE, stringsAsFactors=FALSE)
|
|
|
17
|
|
|
18 SecondSubstrateSet<- read.csv("S2.csv", stringsAsFactors=FALSE)
|
|
|
19 Secondsubbackfreq<- read.csv("SBF2.csv", header=FALSE, stringsAsFactors=FALSE)
|
|
|
20
|
|
|
21 # ThirdSubstrateSet<- read.csv("Galaxy69-BTK_PLUS_R3_Substrates.csv", stringsAsFactors=FALSE)
|
|
|
22 # Thirdsubbackfreq<- read.csv("Galaxy70-BTK_PLUS_R3_SubstrateBackgroundFrequency.csv", header=FALSE, stringsAsFactors=FALSE)
|
|
|
23
|
|
|
24 #then put the names of your output files here
|
|
|
25 # Shared_motifs_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-substrates.csv"
|
|
|
26 # Shared_subbackfreq_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-SubBackFreq.csv"
|
|
|
27
|
|
|
28 # Shared_motifs_table<-"Shared motifs 7-27-17.csv"
|
|
|
29 # Shared_subbackfreq_table<-"SubstrateBackgrounFrequency-for-shared-motifs 4 7-27-17.csv"
|
|
|
30
|
|
|
31 First_unshared_motifs_table<-"1RS.csv"
|
|
|
32 First_unshared_subbackfreq<-"1RSBF.csv"
|
|
|
33
|
|
|
34 Second_unshared_motifs_table<-"2SR.csv"
|
|
|
35 Second_unshared_subbackfreq<-"2RSBF.csv"
|
|
|
36
|
|
|
37 # Third_unshared_motifs_table<-"R3 subs.csv"
|
|
|
38 # Third_unshared_subbackfreq<-"R3 SBF.csv"
|
|
|
39
|
|
|
40 #final note, this code is going to be unworkable if you want to make a Venn diagram of more than 3 circles. I think I'll poke around
|
|
|
41 #other languages to see if any of them can do it.
|
|
|
42 ####################################################################################################################################
|
|
|
43
|
|
|
44
|
|
|
45
|
|
|
46
|
|
|
47
|
|
|
48 # grepl(pattern = "S", x=asdf, ignore.case = TRUE)
|
|
|
49
|
|
|
50 FirstCentralLetters<-FirstSubstrateSet[,11]
|
|
|
51 SecondCentralLetters<-SecondSubstrateSet[,11]
|
|
|
52
|
|
|
53 FirstEsses<-sapply(FirstCentralLetters, grepl, pattern="S", ignore.case=TRUE)
|
|
|
54 FirstTees<-sapply(FirstCentralLetters, grepl, pattern="T", ignore.case=TRUE)
|
|
|
55 FirstWys<-sapply(FirstCentralLetters, grepl, pattern="Y", ignore.case=TRUE)
|
|
|
56
|
|
|
57 SecondEsses<-sapply(SecondCentralLetters, grepl, pattern="S", ignore.case=TRUE)
|
|
|
58 SecondTees<-sapply(SecondCentralLetters, grepl, pattern="T", ignore.case=TRUE)
|
|
|
59 SecondWys<-sapply(SecondCentralLetters, grepl, pattern="Y", ignore.case=TRUE)
|
|
|
60
|
|
|
61 FirstCentralLetters<-replace(FirstCentralLetters,FirstEsses,"xS")
|
|
|
62 FirstCentralLetters<-replace(FirstCentralLetters,FirstTees,"xT")
|
|
|
63 FirstCentralLetters<-replace(FirstCentralLetters,FirstWys,"xY")
|
|
|
64
|
|
|
65 SecondCentralLetters<-replace(SecondCentralLetters,SecondEsses,"xS")
|
|
|
66 SecondCentralLetters<-replace(SecondCentralLetters,SecondTees,"xT")
|
|
|
67 SecondCentralLetters<-replace(SecondCentralLetters,SecondWys,"xY")
|
|
|
68
|
|
|
69 FirstCentralLetters->FirstSubstrateSet[,11]
|
|
|
70 SecondCentralLetters->SecondSubstrateSet[,11]
|
|
|
71
|
|
|
72 ####################################################################################################################################
|
|
|
73 ####################################################################################################################################
|
|
|
74 # better version of this code written in C: what happens when two kinases share a motif, but they found that motif in two
|
|
|
75 # separate proteins thus two separate accession numbers?
|
|
|
76 # It should actually output the shared motif and BOTH accession numbers. Right now it does not, it only maps out the second
|
|
|
77 # accession number. So that needs to be fixed BUT you need to keep the commonality between a motif and its accession number
|
|
|
78 ####################################################################################################################################
|
|
|
79 ####################################################################################################################################
|
|
|
80 ####################################################################################################################################
|
|
|
81 ####################################################################################################################################
|
|
|
82
|
|
|
83 #Create the motif sets, deciding wether or not you're looking for truncated or full here
|
|
|
84 #full only
|
|
|
85
|
|
|
86
|
|
|
87 if (Are_You_Looking_For_Commonality=="NO"){
|
|
|
88
|
|
|
89 ###############################################
|
|
|
90 #ALL motifs, full and truncated
|
|
|
91
|
|
|
92 if (FullMotifsOnly_questionmark!="YES"&&TruncatedMotifsOnly_questionmark!="YES"){
|
|
|
93 FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1)
|
|
|
94 FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1)
|
|
|
95
|
|
|
96 for (i in 1:nrow(FirstSubstrateSet)){
|
|
|
97 FTLwtletters<-FirstSubstrateSet[i,4:18]
|
|
|
98 FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"]
|
|
|
99 FTLwtletters<-paste(FTLwtletters, sep="", collapse="")
|
|
|
100 leftspaces<-c()
|
|
|
101 rightspaces<-c()
|
|
|
102
|
|
|
103 YYYmotif <- unlist(strsplit(FTLwtletters, split = ""))
|
|
|
104 YYYposition <- match(x = "x", table = YYYmotif)
|
|
|
105 #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are
|
|
|
106 #just 3 letters to the left of x
|
|
|
107
|
|
|
108 YYYLettersToTheLeft <- YYYposition - 1
|
|
|
109 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is
|
|
|
110 #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1
|
|
|
111 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1
|
|
|
112 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the
|
|
|
113 #variable the user puts in is
|
|
|
114
|
|
|
115
|
|
|
116 if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) {
|
|
|
117 leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft))
|
|
|
118 rightspaces<-rep(" ",times=7-(YYYLettersToTheRight))
|
|
|
119 #add blank spaces if the motif has less than 4 letters to the left/right
|
|
|
120 motif<-c(leftspaces,YYYmotif,rightspaces)
|
|
|
121 #save that motif, which is the Y and +/- 4 amino acids, including truncation
|
|
|
122 motif<-motif[!motif %in% "x"]
|
|
|
123 motif<-paste(motif, sep="", collapse="")
|
|
|
124 FTLwtletters<-motif
|
|
|
125 FTLwtmotifs[i,1]<-FTLwtletters
|
|
|
126 FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
|
|
|
127 }
|
|
|
128
|
|
|
129 if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){
|
|
|
130 motif<-YYYmotif
|
|
|
131 #add blank spaces if the motif has less than 4 letters to the left/right
|
|
|
132 motif<-c(leftspaces,YYYmotif,rightspaces)
|
|
|
133 #save that motif, which is the Y and +/- 4 amino acids, including truncation
|
|
|
134 motif<-motif[!motif %in% "x"]
|
|
|
135 motif<-paste(motif, sep="", collapse="")
|
|
|
136 FTLwtletters<-motif
|
|
|
137 FTLwtmotifs[i,1]<-FTLwtletters
|
|
|
138 FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
|
|
|
139
|
|
|
140
|
|
|
141 }
|
|
|
142
|
|
|
143 }
|
|
|
144
|
|
|
145 D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1)
|
|
|
146 D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1)
|
|
|
147
|
|
|
148 for (i in 1:nrow(SecondSubstrateSet)){
|
|
|
149 D835letters<-SecondSubstrateSet[i,4:18]
|
|
|
150 D835letters<-D835letters[D835letters !="XXXXX"]
|
|
|
151 D835letters<-paste(D835letters, sep="", collapse="")
|
|
|
152 leftspaces<-c()
|
|
|
153 rightspaces<-c()
|
|
|
154
|
|
|
155 YYYmotif <- unlist(strsplit(D835letters, split = ""))
|
|
|
156 YYYposition <- match(x = "x", table = YYYmotif)
|
|
|
157 #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are
|
|
|
158 #just 3 letters to the left of x
|
|
|
159
|
|
|
160 YYYLettersToTheLeft <- YYYposition - 1
|
|
|
161 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is
|
|
|
162 #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1
|
|
|
163 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1
|
|
|
164 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the
|
|
|
165 #variable the user puts in is
|
|
|
166 if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) {
|
|
|
167 leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft))
|
|
|
168 rightspaces<-rep(" ",times=7-(YYYLettersToTheRight))
|
|
|
169 #add blank spaces if the motif has less than 4 letters to the left/right
|
|
|
170 motif<-c(leftspaces,YYYmotif,rightspaces)
|
|
|
171 #save that motif, which is the Y and +/- 4 amino acids, including truncation
|
|
|
172 motif<-motif[!motif %in% "x"]
|
|
|
173 motif<-paste(motif, sep="", collapse="")
|
|
|
174 D835letters<-motif
|
|
|
175 D835Ymotifs[i,1]<-D835letters
|
|
|
176 D835YAccessionNumbers[i,1]<-SecondSubstrateSet[i,3]
|
|
|
177 }
|
|
|
178
|
|
|
179 if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){
|
|
|
180 motif<-YYYmotif
|
|
|
181 #add blank spaces if the motif has less than 4 letters to the left/right
|
|
|
182 motif<-c(leftspaces,YYYmotif,rightspaces)
|
|
|
183 #save that motif, which is the Y and +/- 4 amino acids, including truncation
|
|
|
184 motif<-motif[!motif %in% "x"]
|
|
|
185 motif<-paste(motif, sep="", collapse="")
|
|
|
186 D835letters<-motif
|
|
|
187 D835Ymotifs[i,1]<-D835letters
|
|
|
188 D835YAccessionNumbers[i,1]<-SecondSubstrateSet[i,3]
|
|
|
189 }
|
|
|
190 }
|
|
|
191
|
|
|
192 names(FTLwtmotifs)<-FTLwtAccessionNumbers
|
|
|
193 names(D835Ymotifs)<-D835YAccessionNumbers
|
|
|
194
|
|
|
195 }
|
|
|
196
|
|
|
197
|
|
|
198 FTLwtmotifsFINAL<-FTLwtmotifs[!FTLwtmotifs %in% D835Ymotifs]
|
|
|
199 FTLwtmotifsFINAL<-FTLwtmotifsFINAL[!duplicated(FTLwtmotifsFINAL)]
|
|
|
200
|
|
|
201 D835YmotifsFINAL<-D835Ymotifs[!D835Ymotifs %in% FTLwtmotifs]
|
|
|
202 D835YmotifsFINAL<-D835YmotifsFINAL[!duplicated(D835YmotifsFINAL)]
|
|
|
203
|
|
|
204
|
|
|
205 columnalheader<-c(rep(NA,36))
|
|
|
206 FTLFinalMatrix<-matrix(data =columnalheader,nrow = 1)
|
|
|
207
|
|
|
208 for (k in 1:length(FTLwtmotifsFINAL)) {
|
|
|
209 AN<-00000
|
|
|
210 #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is
|
|
|
211 #destroyed immediately after use
|
|
|
212 for (m in 1:ncol(Firstsubbackfreq)) {
|
|
|
213 AN <- as.character(Firstsubbackfreq[1, m])
|
|
|
214 if (grepl(pattern = AN,
|
|
|
215 x = names(FTLwtmotifsFINAL[k]),
|
|
|
216 fixed = TRUE) == TRUE) {
|
|
|
217 outputmatrix <- as.character(Firstsubbackfreq[, m])
|
|
|
218 outputmatrix <- matrix(outputmatrix, nrow = 1)
|
|
|
219 #with that accession number, find a match in the subbackfreq file and save it here
|
|
|
220 FTLFinalMatrix<-rbind(FTLFinalMatrix,outputmatrix)
|
|
|
221 }
|
|
|
222 }
|
|
|
223 }
|
|
|
224 FTLFinalMatrix<-FTLFinalMatrix[!duplicated(FTLFinalMatrix),]
|
|
|
225 FTLFinalMatrix<-FTLFinalMatrix[2:nrow(FTLFinalMatrix),]
|
|
|
226
|
|
|
227 columnalheader<-c(rep(NA,36))
|
|
|
228 D835YFinalMatrix<-matrix(data =columnalheader,nrow = 1)
|
|
|
229
|
|
|
230 for (k in 1:length(D835YmotifsFINAL)) {
|
|
|
231 #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is
|
|
|
232 #destroyed immediately after use
|
|
|
233 for (m in 1:ncol(Secondsubbackfreq)) {
|
|
|
234 AN <- as.character(Secondsubbackfreq[1, m])
|
|
|
235 if (grepl(pattern = AN,
|
|
|
236 x = names(D835YmotifsFINAL[k]),
|
|
|
237 fixed = TRUE) == TRUE) {
|
|
|
238 outputmatrix <- as.character(Secondsubbackfreq[, m])
|
|
|
239 outputmatrix <- matrix(outputmatrix, nrow = 1)
|
|
|
240 #with that accession number, find a match in the subbackfreq file and save it here
|
|
|
241 D835YFinalMatrix<-rbind(D835YFinalMatrix,outputmatrix)
|
|
|
242 }
|
|
|
243 }
|
|
|
244 }
|
|
|
245 D835YFinalMatrix<-D835YFinalMatrix[!duplicated(D835YFinalMatrix),]
|
|
|
246 D835YFinalMatrix<-D835YFinalMatrix[2:nrow(D835YFinalMatrix),]
|
|
|
247
|
|
|
248 FTLoutputmatrix<-matrix(data=c(FTLwtmotifsFINAL,names(FTLwtmotifsFINAL)),ncol = 2)
|
|
|
249
|
|
|
250
|
|
|
251 FLTheader<-c("Substrate","Species","Reference","-7","-6","-5","-4","-3","-2","-1","0","1","2","3","4","5","6","7","Phosphite")
|
|
|
252 # FLTheader<-unlist(FLTheader)
|
|
|
253 lefthandFLT<-matrix(data = rep(NA,times=2*nrow(FTLoutputmatrix)),nrow=nrow(FTLoutputmatrix))
|
|
|
254 righthandFLT<-matrix(data = rep(NA,times=1*nrow(FTLoutputmatrix)),nrow=nrow(FTLoutputmatrix))
|
|
|
255 FLTaccessionset<-FTLoutputmatrix[,2]
|
|
|
256 FTLmeat<-sapply(FTLoutputmatrix[,1], strsplit, "")
|
|
|
257 FTLmeat<-sapply(FTLmeat, unlist)
|
|
|
258 colnames(FTLmeat)<-NULL
|
|
|
259 FTLmeat<-t(FTLmeat)
|
|
|
260
|
|
|
261 FTLoutputmatrix2<-cbind(lefthandFLT,FLTaccessionset,FTLmeat,righthandFLT)
|
|
|
262 colnames(FTLoutputmatrix2)<-NULL
|
|
|
263 rownames(FTLoutputmatrix2)<-NULL
|
|
|
264 colnames(FLTheader)<-NULL
|
|
|
265 rownames(FLTheader)<-NULL
|
|
|
266
|
|
|
267
|
|
|
268 FirstCentralLettersAGAIN<-FTLoutputmatrix2[,11]
|
|
|
269
|
|
|
270 FirstEsses<-sapply(FirstCentralLettersAGAIN, grepl, pattern="S", ignore.case=TRUE)
|
|
|
271 FirstTees<-sapply(FirstCentralLettersAGAIN, grepl, pattern="T", ignore.case=TRUE)
|
|
|
272 FirstWys<-sapply(FirstCentralLettersAGAIN, grepl, pattern="Y", ignore.case=TRUE)
|
|
|
273
|
|
|
274 FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstEsses,"xS")
|
|
|
275 FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstTees,"xT")
|
|
|
276 FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstWys,"xY")
|
|
|
277
|
|
|
278 FirstCentralLettersAGAIN->FTLoutputmatrix2[,11]
|
|
|
279
|
|
|
280 FTLoutputmatrix2<-rbind(FLTheader,FTLoutputmatrix2)
|
|
|
281
|
|
|
282 write.table(x=FTLoutputmatrix2,
|
|
|
283 file=First_unshared_motifs_table,
|
|
|
284 quote=FALSE, sep=",",
|
|
|
285 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
286
|
|
|
287 columnalheader<-c("Accession Numbers",as.character(Firstsubbackfreq[1:35,1]))
|
|
|
288 columnalheader<-matrix(columnalheader,nrow = 1)
|
|
|
289 write.table(x=columnalheader,
|
|
|
290 file=First_unshared_subbackfreq,
|
|
|
291 quote=FALSE, sep=",",
|
|
|
292 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
293
|
|
|
294 write.table(x=FTLFinalMatrix,
|
|
|
295 file=First_unshared_subbackfreq,
|
|
|
296 quote=FALSE, sep=",",
|
|
|
297 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
298
|
|
|
299 ############################################################################################################
|
|
|
300
|
|
|
301 D835Youtputmatrix<-matrix(data=c(D835YmotifsFINAL,names(D835YmotifsFINAL)),ncol = 2)
|
|
|
302
|
|
|
303 D835Yheader<-c("Substrate","Species","Reference","-7","-6","-5","-4","-3","-2","-1","0","1","2","3","4","5","6","7","Phosphite")
|
|
|
304 # D835Yheader<-unlist(D835Yheader)
|
|
|
305 lefthandD835<-matrix(data = rep(NA,times=2*nrow(D835Youtputmatrix)),nrow=nrow(D835Youtputmatrix))
|
|
|
306 righthandD835<-matrix(data = rep(NA,times=1*nrow(D835Youtputmatrix)),nrow=nrow(D835Youtputmatrix))
|
|
|
307 D835Yaset<-D835Youtputmatrix[,2]
|
|
|
308 D835meat<-sapply(D835Youtputmatrix[,1], strsplit, "")
|
|
|
309 D835meat<-sapply(D835meat, unlist)
|
|
|
310 colnames(D835meat)<-NULL
|
|
|
311 D835meat<-t(D835meat)
|
|
|
312
|
|
|
313 D835Youtputmatrix2<-cbind(lefthandD835,D835Yaset,D835meat,righthandD835)
|
|
|
314 colnames(D835Youtputmatrix2)<-NULL
|
|
|
315 rownames(D835Youtputmatrix2)<-NULL
|
|
|
316 colnames(D835Yheader)<-NULL
|
|
|
317 rownames(D835Yheader)<-NULL
|
|
|
318
|
|
|
319
|
|
|
320 SecondCentralLettersAGAIN<-D835Youtputmatrix2[,11]
|
|
|
321
|
|
|
322 SecondEsses<-sapply(SecondCentralLettersAGAIN, grepl, pattern="S", ignore.case=TRUE)
|
|
|
323 SecondTees<-sapply(SecondCentralLettersAGAIN, grepl, pattern="T", ignore.case=TRUE)
|
|
|
324 SecondWys<-sapply(SecondCentralLettersAGAIN, grepl, pattern="Y", ignore.case=TRUE)
|
|
|
325
|
|
|
326 SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondEsses,"xS")
|
|
|
327 SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondTees,"xT")
|
|
|
328 SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondWys,"xY")
|
|
|
329
|
|
|
330 SecondCentralLettersAGAIN->D835Youtputmatrix2[,11]
|
|
|
331
|
|
|
332 D835Youtputmatrix2<-rbind(D835Yheader,D835Youtputmatrix2)
|
|
|
333
|
|
|
334 write.table(x=D835Youtputmatrix2,
|
|
|
335 file=Second_unshared_motifs_table,
|
|
|
336 quote=FALSE, sep=",",
|
|
|
337 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
338
|
|
|
339 columnalheader<-c("Accession Numbers",as.character(Firstsubbackfreq[1:35,1]))
|
|
|
340 columnalheader<-matrix(columnalheader,nrow = 1)
|
|
|
341 write.table(x=columnalheader,
|
|
|
342 file=Second_unshared_subbackfreq,
|
|
|
343 quote=FALSE, sep=",",
|
|
|
344 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
345
|
|
|
346 write.table(x=D835YFinalMatrix,
|
|
|
347 file=Second_unshared_subbackfreq,
|
|
|
348 quote=FALSE, sep=",",
|
|
|
349 row.names=FALSE,col.names = FALSE, na="", append=TRUE)
|
|
|
350 }
|
|
|
351
|
