--- /dev/null	Thu Jan 01 00:00:00 1970 +0000
+++ b/all stuff/Difference finderMADE 7 TO 7 fastversion 2-14-2019.R	Fri Apr 19 14:00:46 2019 -0400
@@ -0,0 +1,453 @@
+#I should make an SOP for this.  Problems we encountered: no x in the xY motif, and the kilodemon
+#the output files have both Y and xY, they shouldn't why is that happening?  make it not happen
+#make sure that accession numbers stay locked to each motif, somehow
+#output should look just like the KALIP input
+#S2 is negatives
+#ff you want ONLY FULL MOTIFS, put "YES" here, please use all caps
+FullMotifsOnly_questionmark<-"NO"
+#If you want ONLY TRUNCATED MOTIFS, put "YES" here, please use all caps
+TruncatedMotifsOnly_questionmark<-"NO"
+#if you want to find the overlap, put a "YES" here (all caps), if you want to find the non-overlap, put "NO" (all caps)
+Are_You_Looking_For_Commonality<-"NO"
+
+
+#put the names of your input files here
+FirstSubstrateSet<- read.csv("Substrates 1A TiO2 and FeNTA no duplicates.csv", stringsAsFactors=FALSE)
+Firstsubbackfreq<- read.csv("SBF 1A TiO2 and FeNTA no duplicates.csv", header=FALSE, stringsAsFactors=FALSE)
+
+SecondSubstrateSet<- read.csv("Substrates 1B TiO2 and FeNTA no duplicates.csv", stringsAsFactors=FALSE)
+Secondsubbackfreq<- read.csv("SBF 1B TiO2 and FeNTA no duplicates.csv", header=FALSE, stringsAsFactors=FALSE)
+
+# ThirdSubstrateSet<- read.csv("Galaxy69-BTK_PLUS_R3_Substrates.csv", stringsAsFactors=FALSE)
+# Thirdsubbackfreq<- read.csv("Galaxy70-BTK_PLUS_R3_SubstrateBackgroundFrequency.csv", header=FALSE, stringsAsFactors=FALSE)
+
+#then put the names of your output files here
+# Shared_motifs_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-substrates.csv"
+# Shared_subbackfreq_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-SubBackFreq.csv"
+
+# Shared_motifs_table<-"Shared motifs 7-27-17.csv"
+# Shared_subbackfreq_table<-"SubstrateBackgrounFrequency-for-shared-motifs 4 7-27-17.csv"
+
+First_unshared_motifs_table<-"pka_minus_wo-plus_wo_vector_subs.csv"
+First_unshared_subbackfreq<-"pka_minus_wo-plus_wo_vector_sbf.csv"
+
+Second_unshared_motifs_table<-"pka_plus_wo-minus_wo_vector_subs.csv"
+Second_unshared_subbackfreq<-"pka_plus_wo-minus_wo_vector_sbf.csv"
+
+# Third_unshared_motifs_table<-"R3 subs.csv"
+# Third_unshared_subbackfreq<-"R3 SBF.csv"
+
+#final note, this code is going to be unworkable if you want to make a Venn diagram of more than 3 circles.  I think I'll poke around
+#other languages to see if any of them can do it.
+####################################################################################################################################
+
+
+
+
+
+# grepl(pattern = "S", x=asdf, ignore.case = TRUE)
+
+FirstCentralLetters<-FirstSubstrateSet[,11]
+SecondCentralLetters<-SecondSubstrateSet[,11]
+
+FirstEsses<-sapply(FirstCentralLetters, grepl, pattern="S", ignore.case=TRUE)
+FirstTees<-sapply(FirstCentralLetters, grepl, pattern="T", ignore.case=TRUE)
+FirstWys<-sapply(FirstCentralLetters, grepl, pattern="Y", ignore.case=TRUE)
+
+SecondEsses<-sapply(SecondCentralLetters, grepl, pattern="S", ignore.case=TRUE)
+SecondTees<-sapply(SecondCentralLetters, grepl, pattern="T", ignore.case=TRUE)
+SecondWys<-sapply(SecondCentralLetters, grepl, pattern="Y", ignore.case=TRUE)
+
+FirstCentralLetters<-replace(FirstCentralLetters,FirstEsses,"xS")
+FirstCentralLetters<-replace(FirstCentralLetters,FirstTees,"xT")
+FirstCentralLetters<-replace(FirstCentralLetters,FirstWys,"xY")
+
+SecondCentralLetters<-replace(SecondCentralLetters,SecondEsses,"xS")
+SecondCentralLetters<-replace(SecondCentralLetters,SecondTees,"xT")
+SecondCentralLetters<-replace(SecondCentralLetters,SecondWys,"xY")
+
+FirstCentralLetters->FirstSubstrateSet[,11]
+SecondCentralLetters->SecondSubstrateSet[,11]
+
+####################################################################################################################################
+####################################################################################################################################
+# better version of this code written in C: what happens when two kinases share a motif, but they found that motif in two 
+# separate proteins thus two separate accession numbers?
+# It should actually output the shared motif and BOTH accession numbers.  Right now it does not, it only maps out the second
+# accession number.  So that needs to be fixed BUT you need to keep the commonality between a motif and its accession number
+####################################################################################################################################
+####################################################################################################################################
+####################################################################################################################################
+####################################################################################################################################
+
+#Create the motif sets, deciding wether or not you're looking for truncated or full here
+#full only
+
+
+if (Are_You_Looking_For_Commonality=="NO"){
+  
+  ###############################################
+  #ALL motifs, full and truncated
+  
+  if (FullMotifsOnly_questionmark!="YES"&&TruncatedMotifsOnly_questionmark!="YES"){
+    # FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1)
+    # FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1)
+    
+    #so what this does is
+    #find the central phospho position and add spaces to either side of it
+    #create a pasted together motif which will be used for difference finding
+    #keep motifs and accession numbers in the same index position of two vectors
+    
+    #so instead just take the FLTwt[i,4:18], and replace "" with "O"
+    #then perform paste function
+    #then FLTwt with "O" can be the motif that will get printed to the CSV (after re-removing the "O") and the pasted motif is in its own vector that follows the A #s
+    
+    FTLwtmotifs<-FirstSubstrateSet[,4:18]
+    # FTLwtmotifs<-sapply(FTLwtmotifs, gsub, pattern=" ", replacement="O",ignore.case=TRUE)
+    FTLwtmotifs<-replace(FTLwtmotifs, FTLwtmotifs=="", "O")
+    FTLwtmotifs<-apply(FTLwtmotifs, 1, paste, sep="", collapse="")
+    FTLwtAccessionNumbers<-FirstSubstrateSet[,3]
+    
+    # D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1)
+    # D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1)
+    
+    D835Ymotifs<-SecondSubstrateSet[,4:18]
+    # D835Ymotifs<-sapply(D835Ymotifs, gsub, patter="", replacement="O",ignore.case=TRUE)
+    D835Ymotifs<-replace(D835Ymotifs, D835Ymotifs=="", "O")
+    D835Ymotifs<-replace(D835Ymotifs, D835Ymotifs=="", "O")
+    D835Ymotifs<-apply(D835Ymotifs, 1, paste, sep="", collapse="")
+    D835YAccessionNumbers<-SecondSubstrateSet[,3]
+    
+    names(FTLwtmotifs)<-FTLwtAccessionNumbers
+    names(D835Ymotifs)<-D835YAccessionNumbers
+    
+  }
+  
+  
+  FTLwtmotifsFINAL<-FTLwtmotifs[!FTLwtmotifs %in% D835Ymotifs]
+  FTLwtmotifsFINAL<-FTLwtmotifsFINAL[!duplicated(FTLwtmotifsFINAL)]
+  
+  D835YmotifsFINAL<-D835Ymotifs[!D835Ymotifs %in% FTLwtmotifs]
+  D835YmotifsFINAL<-D835YmotifsFINAL[!duplicated(D835YmotifsFINAL)]
+  
+  
+  
+  # columnalheader<-c(rep(NA,36))
+  # FTLFinalMatrix<-matrix(data =columnalheader,nrow = 1)
+  # 
+  #how to make this an apply
+  #actual first turn names(motifsFINAL) into a vector, then gsub to replace all "|" pipe characters with "*" star characters
+  #first take the accession numbers as Firstsubbackfreq[1,], then gsub to replace all "|" pipe characters with "*" star characters
+  #then use AccessionNumbers as a vector and use 
+  #matches <- unique (grep(paste(toMatch,collapse="|"), names(motifsFINAL), value=TRUE))
+  
+  FLTwtNames<-names(FTLwtmotifsFINAL)
+  FLTwtNames<-FLTwtNames[!duplicated(FLTwtNames)]
+  #the problem is the names includes a lot of things that are multi names separated by ;
+  #I need to do soem paper writing and poster writing
+  
+  FLTwtNames<-gsub(pattern = '\\|', replacement = "*", x=FLTwtNames)
+  FLTwtNames<-paste(FLTwtNames,collapse = "|")
+  
+  AccNums<-as.character(Firstsubbackfreq[1,])
+  AccNums<-gsub(pattern = "\\|", replacement = "*", x=AccNums)
+  
+  
+  firstthree123<-gsub(pattern = "\\|", replacement = "", x=FLTwtNames)
+  lastthree123<-gsub(pattern = "\\|", replacement = "", x=AccNums)
+
+  pasty<-paste(lastthree123,collapse = "|")
+  
+  my_matches<-unique(grep(pattern = FLTwtNames, x=AccNums, value = TRUE))
+  # matches <- unique (grep(paste(FLTwtNames,collapse="|"), AccNums, value=TRUE))
+  My_matches<- AccNums %in% FLTwtNames #use apply grep function
+  My_matches<-grep(pattern = pasty, x=firstthree123, ignore.case = TRUE)
+  sensation<-firstthree123[My_matches]
+  what<-firstthree123[!firstthree123 %in% sensation]
+  
+  #my matches are which columns in the SBF file still have a motif with them
+  FirstFINALSBF<-Firstsubbackfreq[My_matches]
+  FTLFinalMatrix<-FirstFINALSBF
+  duped<-FTLFinalMatrix[1,]
+  duped<-duped[!duplicated(duped)]
+  names(duped)<-NULL
+  duped<-unlist(duped)
+  unduped<-FTLFinalMatrix[,!duplicated(duped)]
+  FTLFinalMatrix<-unduped
+  # FTLFinalMatrix<-FTLFinalMatrix[2:nrow(FTLFinalMatrix),]
+  
+  # columnalheader<-c(rep(NA,36))
+  # D835YFinalMatrix<-matrix(data =columnalheader,nrow = 1)
+  
+  D835names<-names(D835YmotifsFINAL)
+  D835names<-D835names[!duplicated(D835names)]
+  AccNums<-as.character(Secondsubbackfreq[1,])
+  My_matches<-AccNums %in% D835names
+  SecondFINALSBF<-Secondsubbackfreq[My_matches]
+  D835YFinalMatrix<-SecondFINALSBF
+  duped<-D835YFinalMatrix[1,]
+  duped<-duped[!duplicated(duped)]
+  names(duped)<-NULL
+  duped<-unlist(duped)
+  unduped<-D835YFinalMatrix[,!duplicated(duped)]
+  D835YFinalMatrix<-unduped
+  #there's a problem, accession numbers are duplicated
+  
+  # FTLoutputmatrix<-matrix(data=c(FTLwtmotifsFINAL,names(FTLwtmotifsFINAL)),ncol = 2)
+  
+  FTLwtmotifsFINAL<-gsub(pattern = "O", replacement = " ", x=FTLwtmotifsFINAL)
+  FTLwtmotifsFINAL<-gsub(pattern = "x", replacement = "", x=FTLwtmotifsFINAL)
+  FTLoutputmatrix<-matrix(data = c(FTLwtmotifsFINAL,names(FTLwtmotifsFINAL)), ncol=2)
+  
+  # FTLwtmotifsFINAL<-gsub(pattern = "O", replacement = " ", x=FTLwtmotifsFINAL)
+  # FTLwtmotifsFINAL<-gsub(pattern = "x", replacement = "", x=FTLwtmotifsFINAL)
+  # FTLoutputmatrix<-matrix(data = c(FTLwtmotifsFINAL,names(FTLwtmotifsFINAL)), ncol=2)
+  
+  FLTheader<-c("Substrate","Species","Reference","-7","-6","-5","-4","-3","-2","-1","0","1","2","3","4","5","6","7","Phosphite")
+  # FLTheader<-unlist(FLTheader)
+  lefthandFLT<-matrix(data = rep(NA,times=2*nrow(FTLoutputmatrix)),nrow=nrow(FTLoutputmatrix))
+  righthandFLT<-matrix(data = rep(NA,times=1*nrow(FTLoutputmatrix)),nrow=nrow(FTLoutputmatrix))
+  FLTaccessionset<-FTLoutputmatrix[,2]
+  FTLmeat<-sapply(FTLoutputmatrix[,1], strsplit, "")
+  FTLmeat<-sapply(FTLmeat, unlist)
+  colnames(FTLmeat)<-NULL
+  FTLmeat<-t(FTLmeat)
+  
+  FTLoutputmatrix2<-cbind(lefthandFLT,FLTaccessionset,FTLmeat,righthandFLT)
+  colnames(FTLoutputmatrix2)<-NULL
+  rownames(FTLoutputmatrix2)<-NULL
+  colnames(FLTheader)<-NULL
+  rownames(FLTheader)<-NULL
+  
+  
+  FirstCentralLettersAGAIN<-FTLoutputmatrix2[,11]
+  
+  FirstEsses<-sapply(FirstCentralLettersAGAIN, grepl, pattern="S", ignore.case=TRUE)
+  FirstTees<-sapply(FirstCentralLettersAGAIN, grepl, pattern="T", ignore.case=TRUE)
+  FirstWys<-sapply(FirstCentralLettersAGAIN, grepl, pattern="Y", ignore.case=TRUE)
+  
+  FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstEsses,"xS")
+  FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstTees,"xT")
+  FirstCentralLettersAGAIN<-replace(FirstCentralLettersAGAIN,FirstWys,"xY")
+  
+  FirstCentralLettersAGAIN->FTLoutputmatrix2[,11]
+  
+  FTLoutputmatrix2<-rbind(FLTheader,FTLoutputmatrix2)
+  
+  write.table(x=FTLoutputmatrix2,
+              file=First_unshared_motifs_table,
+              quote=FALSE, sep=",",
+              row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+  
+  columnalheader<-c("Accession Numbers",as.character(Firstsubbackfreq[1:35,1]))
+  columnalheader<-matrix(columnalheader,ncol = 1)
+  # write.table(x=columnalheader,
+  #             file=First_unshared_subbackfreq,
+  #             quote=FALSE, sep=",",
+  #             row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+  
+  FTLFinalMatrix<-cbind(columnalheader,FTLFinalMatrix)
+  
+  write.table(x=FTLFinalMatrix,
+              file=First_unshared_subbackfreq,
+              quote=FALSE, sep=",",
+              row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+  
+  ############################################################################################################
+  
+  D835YmotifsFINAL<-gsub(pattern = "O", replacement = " ", x=D835YmotifsFINAL)
+  D835YmotifsFINAL<-gsub(pattern = "x", replacement = "", x=D835YmotifsFINAL)
+  D835Youtputmatrix<-matrix(data = c(D835YmotifsFINAL,names(D835YmotifsFINAL)), ncol=2)
+  
+  # D835YmotifsFINAL<-gsub(pattern = "O", replacement = " ", x=D835YoutputmatrixL)
+  # D835YmotifsFINAL<-gsub(pattern = "x", replacement = "", x=D835Youtputmatrix)
+  # D835Youtputmatrix<-matrix(data = c(D835Youtputmatrix,names(D835Youtputmatrix)), ncol=2)
+  # 
+
+  # D835Youtputmatrix<-matrix(data=c(D835YmotifsFINAL,names(D835YmotifsFINAL)),ncol = 2)
+  
+  D835Yheader<-c("Substrate","Species","Reference","-7","-6","-5","-4","-3","-2","-1","0","1","2","3","4","5","6","7","Phosphite")
+  # D835Yheader<-unlist(D835Yheader)
+  lefthandD835<-matrix(data = rep(NA,times=2*nrow(D835Youtputmatrix)),nrow=nrow(D835Youtputmatrix))
+  righthandD835<-matrix(data = rep(NA,times=1*nrow(D835Youtputmatrix)),nrow=nrow(D835Youtputmatrix))
+  D835Yaset<-D835Youtputmatrix[,2]
+  D835meat<-sapply(D835Youtputmatrix[,1], strsplit, "")
+  D835meat<-sapply(D835meat, unlist)
+  colnames(D835meat)<-NULL
+  D835meat<-t(D835meat)
+
+  # FTLmeat<-sapply(FTLoutputmatrix[,1], strsplit, "")
+  # FTLmeat<-sapply(FTLmeat, unlist)
+  # colnames(FTLmeat)<-NULL
+  # FTLmeat<-t(FTLmeat)
+
+
+  D835Youtputmatrix2<-cbind(lefthandD835,D835Yaset,D835meat,righthandD835)
+  colnames(D835Youtputmatrix2)<-NULL
+  rownames(D835Youtputmatrix2)<-NULL
+  colnames(D835Yheader)<-NULL
+  rownames(D835Yheader)<-NULL
+
+
+  SecondCentralLettersAGAIN<-D835Youtputmatrix2[,11]
+
+  SecondEsses<-sapply(SecondCentralLettersAGAIN, grepl, pattern="S", ignore.case=TRUE)
+  SecondTees<-sapply(SecondCentralLettersAGAIN, grepl, pattern="T", ignore.case=TRUE)
+  SecondWys<-sapply(SecondCentralLettersAGAIN, grepl, pattern="Y", ignore.case=TRUE)
+
+  SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondEsses,"xS")
+  SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondTees,"xT")
+  SecondCentralLettersAGAIN<-replace(SecondCentralLettersAGAIN,SecondWys,"xY")
+
+  SecondCentralLettersAGAIN->D835Youtputmatrix2[,11]
+
+  D835Youtputmatrix2<-rbind(D835Yheader,D835Youtputmatrix2)
+
+  write.table(x=D835Youtputmatrix2,
+              file=Second_unshared_motifs_table,
+              quote=FALSE, sep=",",
+              row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+  
+  columnalheader<-c("Accession Numbers",as.character(Firstsubbackfreq[1:35,1]))
+  columnalheader<-matrix(columnalheader,ncol = 1)
+  # write.table(x=columnalheader,
+  #             file=Second_unshared_subbackfreq,
+  #             quote=FALSE, sep=",",
+  #             row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+  
+  D835YFinalMatrix<-cbind(columnalheader,D835YFinalMatrix)
+  
+  write.table(x=D835YFinalMatrix,
+              file=Second_unshared_subbackfreq,
+              quote=FALSE, sep=",",
+              row.names=FALSE,col.names = FALSE, na="", append=TRUE)
+}
+
+
+
+#  for (i in 1:nrow(FirstSubstrateSet)){
+#   FTLwtletters<-FirstSubstrateSet[i,4:18]
+#   FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"]
+#   FTLwtletters<-paste(FTLwtletters, sep="", collapse="")
+#   leftspaces<-c()
+#   rightspaces<-c()
+#   
+#   YYYmotif <- unlist(strsplit(FTLwtletters, split = ""))
+#   YYYposition <- match(x = "x", table = YYYmotif)
+#   #position itself tells me how much is to the left of that X by what it's number is.  x at position 4 tells me that there are
+#   #just 3 letters to the left of x
+#   
+#   YYYLettersToTheLeft <- YYYposition - 1
+#   #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is
+#   #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1
+#   YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1
+#   #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the
+#   #variable the user puts in is
+#   
+#   
+#   if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) {
+#     leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft))
+#     rightspaces<-rep(" ",times=7-(YYYLettersToTheRight))
+#     #add blank spaces if the motif has less than 4 letters to the left/right
+#     motif<-c(leftspaces,YYYmotif,rightspaces)
+#     #save that motif, which is the Y and +/- 4 amino acids, including truncation
+#     motif<-motif[!motif %in% "x"]
+#     motif<-paste(motif, sep="", collapse="")
+#     FTLwtletters<-motif
+#     FTLwtmotifs[i,1]<-FTLwtletters
+#     FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
+#   }
+#   
+#   if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){
+#     motif<-YYYmotif
+#     #add blank spaces if the motif has less than 4 letters to the left/right
+#     motif<-c(leftspaces,YYYmotif,rightspaces)
+#     #save that motif, which is the Y and +/- 4 amino acids, including truncation
+#     motif<-motif[!motif %in% "x"]
+#     motif<-paste(motif, sep="", collapse="")
+#     FTLwtletters<-motif
+#     FTLwtmotifs[i,1]<-FTLwtletters
+#     FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
+#     
+#     
+#   }
+#   
+# }
+
+
+
+# for (i in 1:nrow(SecondSubstrateSet)){
+#   D835letters<-SecondSubstrateSet[i,4:18]
+#   D835letters<-D835letters[D835letters !="XXXXX"]
+#   D835letters<-paste(D835letters, sep="", collapse="")
+#   leftspaces<-c()
+#   rightspaces<-c()
+#   
+#   YYYmotif <- unlist(strsplit(D835letters, split = ""))
+#   YYYposition <- match(x = "x", table = YYYmotif)
+#   #position itself tells me how much is to the left of that X by what it's number is.  x at position 4 tells me that there are
+#   #just 3 letters to the left of x
+#   
+#   YYYLettersToTheLeft <- YYYposition - 1
+#   #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is
+#   #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1
+#   YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1
+#   #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the
+#   #variable the user puts in is
+#   if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) {
+#     leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft))
+#     rightspaces<-rep(" ",times=7-(YYYLettersToTheRight))
+#     #add blank spaces if the motif has less than 4 letters to the left/right
+#     motif<-c(leftspaces,YYYmotif,rightspaces)
+#     #save that motif, which is the Y and +/- 4 amino acids, including truncation
+#     motif<-motif[!motif %in% "x"]
+#     motif<-paste(motif, sep="", collapse="")
+#     D835letters<-motif
+#     D835Ymotifs[i,1]<-D835letters
+#     D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
+#   }
+#   
+#   if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){
+#     motif<-YYYmotif
+#     #add blank spaces if the motif has less than 4 letters to the left/right
+#     motif<-c(leftspaces,YYYmotif,rightspaces)
+#     #save that motif, which is the Y and +/- 4 amino acids, including truncation
+#     motif<-motif[!motif %in% "x"]
+#     motif<-paste(motif, sep="", collapse="")
+#     D835letters<-motif
+#     D835Ymotifs[i,1]<-D835letters
+#     D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3]
+#   }
+# }
+
+# for (k in 1:length(FTLwtmotifsFINAL)) {
+#   AN<-00000
+#   #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is
+#   #destroyed immediately after use
+#   for (m in 1:ncol(Firstsubbackfreq)) {
+#     AN <- as.character(Firstsubbackfreq[1, m])
+#     if (grepl(pattern = AN,
+#               x = names(FTLwtmotifsFINAL[k]),
+#               fixed = TRUE) == TRUE) {
+#       outputmatrix <- as.character(Firstsubbackfreq[, m])
+#       outputmatrix <- matrix(outputmatrix, nrow = 1)
+#       #with that accession number, find a match in the subbackfreq file and save it here
+#       FTLFinalMatrix<-rbind(FTLFinalMatrix,outputmatrix)
+#     }
+#   }
+# }
+# for (k in 1:length(D835YmotifsFINAL)) {
+#   #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is
+#   #destroyed immediately after use
+#   for (m in 1:ncol(Secondsubbackfreq)) {
+#     AN <- as.character(Secondsubbackfreq[1, m])
+#     if (grepl(pattern = AN,
+#               x = names(D835YmotifsFINAL[k]),
+#               fixed = TRUE) == TRUE) {
+#       outputmatrix <- as.character(Secondsubbackfreq[, m])
+#       outputmatrix <- matrix(outputmatrix, nrow = 1)
+#       #with that accession number, find a match in the subbackfreq file and save it here
+#       D835YFinalMatrix<-rbind(D835YFinalMatrix,outputmatrix)
+#     }
+#   }
+# }
\ No newline at end of file