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view all stuff/Commonality and Difference finderMADE 7 TO 7.R @ 0:23eea82f5192 draft
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author | jfb |
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date | Wed, 16 Jan 2019 13:55:22 -0500 |
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#I should make an SOP for this. Problems we encountered: no x in the xY motif, and the kilodemon #the output files have both Y and xY, they shouldn't why is that happening? make it not happen #make sure that accession numbers stay locked to each motif, somehow #output should look just like the KALIP input #ff you want ONLY FULL MOTIFS, put "YES" here, please use all caps FullMotifsOnly_questionmark<-"NO" #If you want ONLY TRUNCATED MOTIFS, put "YES" here, please use all caps TruncatedMotifsOnly_questionmark<-"NO" #if you want to find the overlap, put a "YES" here (all caps), if you want to find the non-overlap, put "NO" (all caps) Are_You_Looking_For_Commonality<-"YES" #put the names of your input files here FirstSubstrateSet<- read.csv("Galaxy63-BTK_PLUS-R1_Substrates.csv", stringsAsFactors=FALSE) Firstsubbackfreq<- read.csv("Galaxy64-BTK_PLUS-R1_SubstrateBackgroundFrequency.csv", header=FALSE, stringsAsFactors=FALSE) SecondSubstrateSet<- read.csv("Galaxy65-BTK_PLUS_R2_Substrates.csv", stringsAsFactors=FALSE) Secondsubbackfreq<- read.csv("Galaxy66-BTK_PLUS_R2_SubstrateBackgroundFrequency.csv", header=FALSE, stringsAsFactors=FALSE) ThirdSubstrateSet<- read.csv("Galaxy69-BTK_PLUS_R3_Substrates.csv", stringsAsFactors=FALSE) Thirdsubbackfreq<- read.csv("Galaxy70-BTK_PLUS_R3_SubstrateBackgroundFrequency.csv", header=FALSE, stringsAsFactors=FALSE) #then put the names of your output files here Shared_motifs_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-substrates.csv" Shared_subbackfreq_table<-"180719_GALAXY-BTK-plus-rep-OVLP-7to7-SubBackFreq.csv" # Shared_motifs_table<-"Shared motifs 7-27-17.csv" # Shared_subbackfreq_table<-"SubstrateBackgrounFrequency-for-shared-motifs 4 7-27-17.csv" First_unshared_motifs_table<-"R1 substrates.csv" First_unshared_subbackfreq<-"R1 SBF.csv" Second_unshared_motifs_table<-"R2 subs.csv" Second_unshared_subbackfreq<-"R2 SBf.csv" Third_unshared_motifs_table<-"R3 subs.csv" Third_unshared_subbackfreq<-"R3 SBF.csv" #final note, this code is going to be unworkable if you want to make a Venn diagram of more than 3 circles. I think I'll poke around #other languages to see if any of them can do it. #################################################################################################################################### FirstxY<-rep("xY",times=nrow(FirstSubstrateSet)) FirstSubstrateSet[,11]<-FirstxY SecondxY<-rep("xY",times=nrow(SecondSubstrateSet)) SecondSubstrateSet[,11]<-SecondxY ThirdxY<-rep("xY",times=nrow(ThirdSubstrateSet)) ThirdSubstrateSet[,11]<-ThirdxY #################################################################################################################################### #################################################################################################################################### # better version of this code written in C: what happens when two kinases share a motif, but they found that motif in two # separate proteins thus two separate accession numbers? # It should actually output the shared motif and BOTH accession numbers. Right now it does not, it only maps out the second # accession number. So that needs to be fixed BUT you need to keep the commonality between a motif and its accession number #################################################################################################################################### #################################################################################################################################### #################################################################################################################################### #################################################################################################################################### #Create the motif sets, deciding wether or not you're looking for truncated or full here #full only if (Are_You_Looking_For_Commonality=="YES"){ if (FullMotifsOnly_questionmark=="YES"){ FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) leftspaces<-c() rightspaces<-c() for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } # FTLwtmotifs <- FTLwtmotifs[!is.na(FTLwtmotifs)] # FTLwtmotifs<-matrix(FTLwtmotifs,ncol = 1) # D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1) D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1) for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i,1]<-D835letters D835YAccessionNumbers[i,1]<-SecondSubstrateSet[i,3] } } ITDmotifs=matrix(,nrow = nrow(ThirdSubstrateSet),ncol=1) ITDAccessionNumbers<-matrix(,nrow = nrow(ThirdSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i,1]<-ITDletters ITDAccessionNumbers[i,1]<-ThirdSubstrateSet[i,3] } } } ##############################################3 #Truncated only if (TruncatedMotifsOnly_questionmark=="YES"){ FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1) D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1) for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835YAccessionNumbers[i,1]<-SecondSubstrateSet[i,3] D835Ymotifs[i,1]<-D835letters } } ITDmotifs=matrix(,nrow = nrow(ThirdSubstrateSet),ncol=1) ITDAccessionNumbers<-matrix(,nrow = nrow(ThirdSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDAccessionNumbers[i,1]<-ThirdSubstrateSet[i,3] ITDmotifs[i,1]<-ITDletters } } } ############################################### #ALL motifs, full and truncated if (FullMotifsOnly_questionmark!="YES"&&TruncatedMotifsOnly_questionmark!="YES"){ FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") leftspaces<-c() rightspaces<-c() YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1) D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1) for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") leftspaces<-c() rightspaces<-c() YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i,1]<-D835letters D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i,1]<-D835letters D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } ITDmotifs=matrix(,nrow = nrow(ThirdSubstrateSet),ncol=1) ITDAccessionNumbers<-matrix(,nrow = nrow(ThirdSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) leftspaces<-c() rightspaces<-c() YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i,1]<-ITDletters ITDAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i,1]<-ITDletters ITDAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } } ############################################################################################################################# ############################################################################################################################# ############################################################################################################################# ############################################################################################################################# ############################################################################################################################# #now look for either commonality or difference. Actually could you look for both... if (Are_You_Looking_For_Commonality=="YES"){ columnalheader<-c(as.character(Thirdsubbackfreq[1:36,1])) columnalheader<-matrix(columnalheader,nrow = 1) write.table(x=columnalheader, file=Shared_subbackfreq_table, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) FirstOverlapmotifs<-c() for (i in 1:nrow(ITDmotifs)){ for (j in 1:nrow(D835Ymotifs)){ if (is.na(ITDmotifs[i,1])!=TRUE&&is.na(D835Ymotifs[j,1])!=TRUE){ if (ITDmotifs[i,1]==D835Ymotifs[j,1]){ FirstOverlapmotifs<-c(FirstOverlapmotifs,D835Ymotifs[j,1]) } } } } AllAccessionNumbers<-c() columnalheader<-c(rep(NA,36)) FinalMatrix<-matrix(data =columnalheader,nrow = 1) FinalMotifs<-c(rep(NA,20)) FinalMotifsMatrix<-matrix(data = FinalMotifs,nrow = 1) for (l in 1:length(FirstOverlapmotifs)) { AccessionNumber<-00000000000 for (k in 1:nrow(FTLwtmotifs)) { AccessionNumber<-0000000000000 if(is.na(FTLwtmotifs[k])!=TRUE){ #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is #destroyed immediately after use if (FirstOverlapmotifs[l] == FTLwtmotifs[k]) { substratematrix<-FirstSubstrateSet[k,1:20] substratematrix<-as.matrix(substratematrix,nrow=1) FinalMotifsMatrix<-rbind(FinalMotifsMatrix,substratematrix) #when you find a match between the venn diagrams, save the substrate info you get into a matrix AccessionNumber <- as.character(FirstSubstrateSet[k, 3]) #then take the accession number for (m in 1:ncol(Firstsubbackfreq)) { AN <- as.character(Firstsubbackfreq[1, m]) if (grepl(pattern = AN, x = AccessionNumber, fixed = TRUE) == TRUE) { outputmatrix <- as.character(Firstsubbackfreq[, m]) outputmatrix <- matrix(outputmatrix, nrow = 1) #with that accession number, find a match in the subbackfreq file and save it here FinalMatrix<-rbind(FinalMatrix,outputmatrix) } } } } } } TrueMatrix<-FinalMatrix[!duplicated(FinalMatrix),] TrueFinalMotifsMatrix<-FinalMotifsMatrix[!duplicated(FinalMotifsMatrix),] TrueFinalMotifsMatrix<-TrueFinalMotifsMatrix[2:nrow(TrueFinalMotifsMatrix),] TrueMatrix<-TrueMatrix[2:nrow(TrueMatrix),] write.table( x = TrueFinalMotifsMatrix, file = Shared_motifs_table, quote = FALSE, sep = ",", row.names = FALSE, col.names = TRUE, na = "", append = TRUE ) write.table( x = TrueMatrix, file = Shared_subbackfreq_table, quote = FALSE, sep = ",", row.names = FALSE, col.names = FALSE, na = "", append = TRUE ) } } if (Are_You_Looking_For_Commonality=="NO"){ if (FullMotifsOnly_questionmark=="YES"){ FTLwtmotifs=rep(NA,times=nrow(FirstSubstrateSet)) FTLwtAccessionNumbers=rep(NA,times=nrow(FirstSubstrateSet)) leftspaces<-c() rightspaces<-c() for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i]<-FTLwtletters FTLwtAccessionNumbers[i]<-FirstSubstrateSet[i,3] } } # FTLwtmotifs <- FTLwtmotifs[!is.na(FTLwtmotifs)] # FTLwtmotifs<-matrix(FTLwtmotifs,ncol = 1) # D835Ymotifs=rep(NA,times=nrow(FirstSubstrateSet)) D835YAccessionNumbers=rep(NA,times=nrow(FirstSubstrateSet)) for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i]<-D835letters D835YAccessionNumbers[i]<-SecondSubstrateSet[i,3] } } ITDmotifs=rep(NA,times=nrow(FirstSubstrateSet)) ITDAccessionNumbers=rep(NA,times=nrow(FirstSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft > 6 && YYYLettersToTheRight > 6) { motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i]<-ITDletters ITDAccessionNumbers[i]<-ThirdSubstrateSet[i,3] } } names(ITDmotifs)<-ITDAccessionNumbers names(D835Ymotifs)<-D835YAccessionNumbers names(FTLwtmotifs)<-FTLwtAccessionNumbers } ##############################################3 #Truncated only if (TruncatedMotifsOnly_questionmark=="YES"){ FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1) D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1) i=2 for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835YAccessionNumbers[i,1]<-SecondSubstrateSet[i,3] D835Ymotifs[i,1]<-D835letters } } ITDmotifs=matrix(,nrow = nrow(ThirdSubstrateSet),ncol=1) ITDAccessionNumbers<-matrix(,nrow = nrow(ThirdSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDAccessionNumbers[i,1]<-ThirdSubstrateSet[i,3] ITDmotifs[i,1]<-ITDletters } } names(FTLwtmotifs)<-FTLwtAccessionNumbers names(D835Ymotifs)<-D835YAccessionNumbers names(ITDmotifs)<-ITDAccessionNumbers } ############################################### #ALL motifs, full and truncated if (FullMotifsOnly_questionmark!="YES"&&TruncatedMotifsOnly_questionmark!="YES"){ FTLwtmotifs=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) FTLwtAccessionNumbers=matrix(,nrow = nrow(FirstSubstrateSet),ncol=1) for (i in 1:nrow(FirstSubstrateSet)){ FTLwtletters<-FirstSubstrateSet[i,4:18] FTLwtletters<-FTLwtletters[FTLwtletters !="XXXXX"] FTLwtletters<-paste(FTLwtletters, sep="", collapse="") leftspaces<-c() rightspaces<-c() YYYmotif <- unlist(strsplit(FTLwtletters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") FTLwtletters<-motif FTLwtmotifs[i,1]<-FTLwtletters FTLwtAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } D835Ymotifs=matrix(,nrow = nrow(SecondSubstrateSet),ncol=1) D835YAccessionNumbers<-matrix(,nrow = nrow(SecondSubstrateSet),ncol = 1) for (i in 1:nrow(SecondSubstrateSet)){ D835letters<-SecondSubstrateSet[i,4:18] D835letters<-D835letters[D835letters !="XXXXX"] D835letters<-paste(D835letters, sep="", collapse="") leftspaces<-c() rightspaces<-c() YYYmotif <- unlist(strsplit(D835letters, split = "")) YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i,1]<-D835letters D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") D835letters<-motif D835Ymotifs[i,1]<-D835letters D835YAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } ITDmotifs=matrix(,nrow = nrow(ThirdSubstrateSet),ncol=1) ITDAccessionNumbers<-matrix(,nrow = nrow(ThirdSubstrateSet)) for (i in 1:nrow(ThirdSubstrateSet)){ ITDletters<-ThirdSubstrateSet[i,4:18] ITDletters<-ITDletters[ITDletters !="XXXXX"] ITDletters<-paste(ITDletters, sep="", collapse="") YYYmotif <- unlist(strsplit(ITDletters, split = "")) leftspaces<-c() rightspaces<-c() YYYposition <- match(x = "x", table = YYYmotif) #position itself tells me how much is to the left of that X by what it's number is. x at position 4 tells me that there are #just 3 letters to the left of x YYYLettersToTheLeft <- YYYposition - 1 #how many letters to the right SHOULD just be length(motif)-position-1 if it's 5 long and x is at 3 then Y is at 4 and there is #just 1 spot to the right of Y so LettersToTheRight<-1 because 5-3-1=1 YYYLettersToTheRight <- length(YYYmotif) - YYYposition - 1 #then sanity check, we're currently looking only at +/-4, but this spot allows for up to +/- 7 as well, just depends on what the #variable the user puts in is if (YYYLettersToTheLeft < 7 | YYYLettersToTheRight < 7) { leftspaces<-rep(" ",times=(7-YYYLettersToTheLeft)) rightspaces<-rep(" ",times=7-(YYYLettersToTheRight)) #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i,1]<-ITDletters ITDAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } if(YYYLettersToTheLeft>6 && YYYLettersToTheRight>6){ motif<-YYYmotif #add blank spaces if the motif has less than 4 letters to the left/right motif<-c(leftspaces,YYYmotif,rightspaces) #save that motif, which is the Y and +/- 4 amino acids, including truncation motif<-motif[!motif %in% "x"] motif<-paste(motif, sep="", collapse="") ITDletters<-motif ITDmotifs[i,1]<-ITDletters ITDAccessionNumbers[i,1]<-FirstSubstrateSet[i,3] } } names(FTLwtmotifs)<-FTLwtAccessionNumbers names(D835Ymotifs)<-D835YAccessionNumbers names(ITDmotifs)<-ITDAccessionNumbers } FTLwtmotifsFINAL<-FTLwtmotifs[!FTLwtmotifs %in% D835Ymotifs] FTLwtmotifsFINAL<-FTLwtmotifsFINAL[!FTLwtmotifsFINAL %in% ITDmotifs] FTLwtmotifsFINAL<-FTLwtmotifsFINAL[!duplicated(FTLwtmotifsFINAL)] ITDmotifsFINAL<-ITDmotifs[!ITDmotifs %in% D835Ymotifs] ITDmotifsFINAL<-ITDmotifsFINAL[!ITDmotifsFINAL %in% FTLwtmotifs] ITDmotifsFINAL<-ITDmotifsFINAL[!duplicated(ITDmotifsFINAL)] D835YmotifsFINAL<-D835Ymotifs[!D835Ymotifs %in% FTLwtmotifs] D835YmotifsFINAL<-D835YmotifsFINAL[!D835YmotifsFINAL %in% ITDmotifs] D835YmotifsFINAL<-D835YmotifsFINAL[!duplicated(D835YmotifsFINAL)] columnalheader<-c(rep(NA,35)) FTLFinalMatrix<-matrix(data =columnalheader,nrow = 1) for (k in 1:length(FTLwtmotifsFINAL)) { AN<-00000 #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is #destroyed immediately after use for (m in 1:ncol(Firstsubbackfreq)) { AN <- as.character(Firstsubbackfreq[1, m]) if (grepl(pattern = AN, x = names(FTLwtmotifsFINAL[k]), fixed = TRUE) == TRUE) { outputmatrix <- as.character(Firstsubbackfreq[, m]) outputmatrix <- matrix(outputmatrix, nrow = 1) #with that accession number, find a match in the subbackfreq file and save it here FTLFinalMatrix<-rbind(FTLFinalMatrix,outputmatrix) } } } FTLFinalMatrix<-FTLFinalMatrix[!duplicated(FTLFinalMatrix),] columnalheader<-c(rep(NA,35)) ITDFinalMatrix<-matrix(data =columnalheader,nrow = 1) for (k in 1:length(ITDmotifsFINAL)) { AN<-00000 #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is #destroyed immediately after use for (m in 1:ncol(Thirdsubbackfreq)) { AN <- as.character(Thirdsubbackfreq[1, m]) if (grepl(pattern = AN, x = names(ITDmotifsFINAL[k]), fixed = TRUE) == TRUE) { outputmatrix <- as.character(Thirdsubbackfreq[, m]) outputmatrix <- matrix(outputmatrix, nrow = 1) #with that accession number, find a match in the subbackfreq file and save it here ITDFinalMatrix<-rbind(ITDFinalMatrix,outputmatrix) } } } ITDFinalMatrix<-ITDFinalMatrix[!duplicated(ITDFinalMatrix),] columnalheader<-c(rep(NA,35)) D835YFinalMatrix<-matrix(data =columnalheader,nrow = 1) for (k in 1:length(D835YmotifsFINAL)) { #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is #destroyed immediately after use for (m in 1:ncol(Secondsubbackfreq)) { AN <- as.character(Secondsubbackfreq[1, m]) if (grepl(pattern = AN, x = names(D835YmotifsFINAL[k]), fixed = TRUE) == TRUE) { outputmatrix <- as.character(Secondsubbackfreq[, m]) outputmatrix <- matrix(outputmatrix, nrow = 1) #with that accession number, find a match in the subbackfreq file and save it here D835YFinalMatrix<-rbind(D835YFinalMatrix,outputmatrix) } } } D835YFinalMatrix<-D835YFinalMatrix[!duplicated(D835YFinalMatrix),] FTLoutputmatrix<-matrix(data=c(FTLwtmotifsFINAL,names(FTLwtmotifsFINAL)),ncol = 2) write.table(x=FTLoutputmatrix, file=First_unshared_motifs_table, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) columnalheader<-matrix(columnalheader,nrow = 1) write.table(x=columnalheader, file=First_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) write.table(x=FTLFinalMatrix, file=First_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) ############################################################################################################ D835Youtputmatrix<-matrix(data=c(D835YmotifsFINAL,names(D835YmotifsFINAL)),ncol = 2) write.table(x=D835Youtputmatrix, file=Second_unshared_motifs_table, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) columnalheader<-matrix(columnalheader,nrow = 1) write.table(x=columnalheader, file=Second_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) write.table(x=D835YFinalMatrix, file=Second_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) ############################################################################################################ ITDoutputmatrix<-matrix(data = c(ITDmotifsFINAL,names(ITDmotifsFINAL)),ncol = 2) write.table(x=ITDoutputmatrix, file=Third_unshared_motifs_table, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) columnalheader<-matrix(columnalheader,nrow = 1) write.table(x=columnalheader, file=Third_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) write.table(x=ITDFinalMatrix, file=Third_unshared_subbackfreq, quote=FALSE, sep=",", row.names=FALSE,col.names = FALSE, na="", append=TRUE) } # if (Are_You_Looking_For_Commonality=="NO"){ # # # FTLwtmotifsFULLMATRIX<-cbind(FTLwtmotifs,FTLwtAccessionNumbers) # ITDmotifsFULLMATRIX<-cbind(ITDmotifs,ITDAccessionNumbers) # D835YmotifsFULLMATRIX<-cbind(D835Ymotifs,D835YAccessionNumbers) # # ?duplicated # # FTLwtmotifsFINAL<-FTLwtmotifs[!FTLwtmotifs %in% D835Ymotifs] # FTLwtmotifsFINAL<-FTLwtmotifsFINAL[!FTLwtmotifsFINAL %in% ITDmotifs] # FTLwtmotifsFINAL<-matrix(data=FTLwtmotifsFINAL, ncol = 1) # # ITDmotifsFINAL<-ITDmotifs[!ITDmotifs %in% D835Ymotifs] # ITDmotifsFINAL<-ITDmotifsFINAL[!ITDmotifsFINAL %in% FTLwtmotifs] # ITDmotifsFINAL<-matrix(data=ITDmotifsFINAL, ncol = 1) # # D835YmotifsFINAL<-D835Ymotifs[!D835Ymotifs %in% FTLwtmotifs] # D835YmotifsFINAL<-D835YmotifsFINAL[!D835YmotifsFINAL %in% ITDmotifs] # D835YmotifsFINAL<-matrix(data=D835YmotifsFINAL, ncol = 1) # # FTLnondupeAccessionNumbers<-c() # # for (z in 1:nrow(FTLwtmotifsFINAL)) { # for (w in 1:nrow(FTLwtmotifsFULLMATRIX)) { # if (is.na(FTLwtmotifsFULLMATRIX[w,1])!=TRUE) # if (FTLwtmotifsFINAL[z]==FTLwtmotifsFULLMATRIX[w,1]){ # FTLnondupeAccessionNumbers<-c(FTLnondupeAccessionNumbers,FTLwtmotifsFULLMATRIX[w,2]) # } # } # } # # # #find accession numbers here, put a matrix of those things, amino acid %, but only after I've unduped them # FTLnondupeAccessionNumbers<-FTLnondupeAccessionNumbers[!duplicated(FTLnondupeAccessionNumbers)] # # columnalheader<-c(rep(NA,35)) # FTLFinalMatrix<-matrix(data =columnalheader,nrow = 1) # # for (k in 1:length(FTLnondupeAccessionNumbers)) { # #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is # #destroyed immediately after use # for (m in 1:ncol(Firstsubbackfreq)) { # AN <- as.character(Firstsubbackfreq[1, m]) # if (grepl(pattern = AN, # x = FTLnondupeAccessionNumbers[k], # fixed = TRUE) == TRUE) { # outputmatrix <- as.character(Firstsubbackfreq[, m]) # outputmatrix <- matrix(outputmatrix, nrow = 1) # #with that accession number, find a match in the subbackfreq file and save it here # FTLFinalMatrix<-rbind(FTLFinalMatrix,outputmatrix) # } # } # } # ITDnondupeAccessionNumbers<-c() # # for (z in 1:nrow(ITDmotifsFINAL)) { # for (w in 1:nrow(ITDmotifsFULLMATRIX)) { # if (is.na(ITDmotifsFULLMATRIX[w,1])!=TRUE) # if (ITDmotifsFINAL[z]==ITDmotifsFULLMATRIX[w,1]){ # ITDnondupeAccessionNumbers<-c(ITDnondupeAccessionNumbers,ITDmotifsFULLMATRIX[w,2]) # } # } # } # # # #find accession numbers here, put a matrix of those things, amino acid %, but only after I've unduped them # ITDnondupeAccessionNumbers<-ITDnondupeAccessionNumbers[!duplicated(ITDnondupeAccessionNumbers)] # # columnalheader<-c(rep(NA,35)) # ITDFinalMatrix<-matrix(data =columnalheader,nrow = 1) # # for (k in 1:length(ITDnondupeAccessionNumbers)) { # #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is # #destroyed immediately after use # for (m in 1:ncol(Thirdsubbackfreq)) { # AN <- as.character(Thirdsubbackfreq[1, m]) # if (grepl(pattern = AN, # x = ITDnondupeAccessionNumbers[k], # fixed = TRUE) == TRUE) { # outputmatrix <- as.character(Thirdsubbackfreq[, m]) # outputmatrix <- matrix(outputmatrix, nrow = 1) # #with that accession number, find a match in the subbackfreq file and save it here # ITDFinalMatrix<-rbind(ITDFinalMatrix,outputmatrix) # } # } # } # # # D835YnondupeAccessionNumbers<-c() # # for (z in 1:nrow(D835YmotifsFINAL)) { # for (w in 1:nrow(D835YmotifsFULLMATRIX)) { # if (is.na(D835YmotifsFULLMATRIX[w,1])!=TRUE) # if (D835YmotifsFINAL[z]==D835YmotifsFULLMATRIX[w,1]){ # D835YnondupeAccessionNumbers<-c(D835YnondupeAccessionNumbers,D835YmotifsFULLMATRIX[w,2]) # } # } # } # # # #find accession numbers here, put a matrix of those things, amino acid %, but only after I've unduped them # D835YnondupeAccessionNumbers<-D835YnondupeAccessionNumbers[!duplicated(D835YnondupeAccessionNumbers)] # # columnalheader<-c(rep(NA,35)) # D835YFinalMatrix<-matrix(data =columnalheader,nrow = 1) # # for (k in 1:length(D835YnondupeAccessionNumbers)) { # #I don't remember why, but I felt it necessary to destroy the accession number multiple times to ensure it is # #destroyed immediately after use # for (m in 1:ncol(Secondsubbackfreq)) { # AN <- as.character(Secondsubbackfreq[1, m]) # if (grepl(pattern = AN, # x = D835YnondupeAccessionNumbers[k], # fixed = TRUE) == TRUE) { # outputmatrix <- as.character(Secondsubbackfreq[, m]) # outputmatrix <- matrix(outputmatrix, nrow = 1) # #with that accession number, find a match in the subbackfreq file and save it here # D835YFinalMatrix<-rbind(D835YFinalMatrix,outputmatrix) # } # } # } # # # # # FinalFTLmotifs<-FTLwtmotifsFINAL[!duplicated(FTLwtmotifsFINAL)] # # FinalFTLAccessionNumbers<-FTLnondupeAccessionNumbers[!duplicated(FTLnondupeAccessionNumbers)] # # necessaryNAs<-rep(NA,times=(length(FinalFTLmotifs)-length(FinalFTLAccessionNumbers))) # # FinalFTLAccessionNumbers<-c(FinalFTLAccessionNumbers,necessaryNAs) # # TRUEFTLoutputmatrix<-cbind(FinalFTLmotifs,FinalFTLAccessionNumbers) # # TRUEFTLoutputmatrix # # write.table(x=FTLwtmotifsFINAL, # file=First_unshared_motifs_table, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) # columnalheader<-matrix(columnalheader,nrow = 1) # write.table(x=columnalheader, # file=First_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # write.table(x=FTLFinalMatrix, # file=First_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # # # # # # # # # # # # write.table(x=D835YmotifsFINAL, # file=Second_unshared_motifs_table, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) # columnalheader<-matrix(columnalheader,nrow = 1) # write.table(x=columnalheader, # file=Second_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # write.table(x=D835YFinalMatrix, # file=Second_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # # # # # # # # # # # # # # # # write.table(x=ITDmotifsFINAL, # file=Third_unshared_motifs_table, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # columnalheader<-c("Accession Numbers",as.character(Thirdsubbackfreq[1:35,1])) # columnalheader<-matrix(columnalheader,nrow = 1) # write.table(x=columnalheader, # file=Third_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # write.table(x=ITDFinalMatrix, # file=Third_unshared_subbackfreq, # quote=FALSE, sep=",", # row.names=FALSE,col.names = FALSE, na="", append=TRUE) # # # # # # # }