Mercurial > repos > jjjjia > cpo_prediction
diff cpo_mobsuite.xml @ 1:fea89c4d5227 draft
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author | jjjjia |
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date | Thu, 16 Aug 2018 19:27:05 -0400 |
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children | 698579246d0d |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/cpo_mobsuite.xml Thu Aug 16 19:27:05 2018 -0400 @@ -0,0 +1,104 @@ +<tool id="mob_recon" name="MOB-Recon" version="1.4.8"> + <description>Type contigs and extract plasmid sequences</description> + <requirements> + <requirement type="package" version="1.4.8">mob_suite</requirement> + </requirements> + <command detect_errors="exit_code"> + <![CDATA[ + ln -s "${input}" "${input.name}"; + + mob_recon --num_threads \${GALAXY_SLOTS:-4} --infile "${input.name}" + #if str($adv_param.unicycler_contigs) == "True": + --unicycler_contigs + #end if + #if str($adv_param.run_circlator) == "True": + --run_circlator + #end if + #if str($adv_param.min_length_condition.min_length_param) == "True": + --min_length ${adv_param.min_length_condition.min_length_value} + #end if + --run_typer --outdir '.'; + ]]> + </command> + <inputs> + <param name="input" type="data" format="fasta" label="Input" help="FASTA file with contig(s)"/> + <section name="adv_param" title="Advanced parameters" expanded="False"> + <param name="unicycler_contigs" label="Check for circularity flag generated by unicycler in contigs fasta headers" type="select" value="True"> + <option value="True">Yes</option> + <option value="False">No</option> + </param> + <param name="run_circlator" label="Run circlator minums2 pipeline to check for circular contigs" type="select" value="True"> + <option value="True">Yes</option> + <option value="False">No</option> + </param> + <conditional name="min_length_condition"> + <param name="min_length_param" label="Minimum length of contigs to classify" type="select" value="False"> + <option value="False">No</option> + <option value="True">Yes</option> + </param> + <when value="True"> + <param name="min_length_value" type="integer" value="500" min="50"/> + </when> + <when value="False"/> + </conditional> + </section> + </inputs> + <outputs> + <data name="outfile1" format="tabular" from_work_dir="contig_report.txt" label="${tool.name}: Overall contig MOB-recon report"/> + <data name="outfile2" format="tabular" from_work_dir="repetitive_blast_report.txt" label="${tool.name}: Repetitive elements BLAST report"/> + <data name="outfile3" format="fasta" from_work_dir="chromosome.fasta" label="${tool.name}: Chromosomal sequences"/> + <data name="outfile4" format="tabular" from_work_dir="mobtyper_aggregate_report.txt" label="${tool.name}: Aggregate MOB-typer report for all contigs"/> + <data name="outfile5" format="fasta" label="${tool.name}: Plasmid sequence" hidden="true"> + <discover_datasets pattern="plasmid_.+\.(?P<ext>.+)" ext="fasta" visible="false" assign_primary_output="false"/> + </data> + </outputs> + <tests> + <test> + <param name="input" value="plasmid_476.fasta" ftype="fasta"/> + <section name="adv_param"> + <param name="evalue" value="0.00001"/> + <param name="unicycler_contigs" value="True"/> + <param name="run_circlator" value="True"/> + </section> + <output name="outfile1"> + <assert_contents> + <has_text text="NC_019097"/> + </assert_contents> + </output> + </test> + </tests> + <help> + +**Syntax** + +This tool reconstructs individual plasmid sequences from draft genome assemblies using the plasmid reference databases. + +For more information please visit https://github.com/phac-nml/mob-suite/. + +----- + +**Input:** + +A FASTA file with a single or multiple contigs (e.g. a draft genome assembly): + + +**Output:** + +Tab-delimited report listing information for each input contig on its cluster number, possible replicon, relaxase, and repetitive elements types, etc. Refer to https://github.com/phac-nml/mob-suite#mob-recon-contig-report-format for the description of each column. + +Note: Plasmid sequences will not be output if none are found. Some plasmid could be intergrated into a chromosome. + + + </help> + <citations> + <citation type="bibtex"> + @misc{githubmob-suite, + author = {Robertson J, Nash J}, + title = {MOB-Suite: Software tools for clustering, reconstruction and typing of plasmids from draft assemblies.}, + publisher = {GitHub}, + journal = {GitHub repository}, + doi = {10.1099/mgen.0.000206}, + url = {https://github.com/phac-nml/mob-suite} + }</citation> + </citations> +</tool> \ No newline at end of file