defuse: defuse_trinity_analysis.py annotate

annotate defuse_trinity_analysis.py @ 13:3a4876d01c7e draft

Uploaded

author	jjohnson
date	Tue, 12 Dec 2017 10:01:42 -0500
parents	b22f8634ff84
children

rev	line source
11 b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	1 #!/usr/bin/env python
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	2 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	3 #
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	4 #------------------------------------------------------------------------------
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	5 # University of Minnesota
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	6 # Copyright 2014, Regents of the University of Minnesota
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	7 #------------------------------------------------------------------------------
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	8 # Author:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	9 #
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	10 # James E Johnson
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	11 #
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	12 #------------------------------------------------------------------------------
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	13 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	14
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	15
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	16 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	17 This tool takes the defuse results.tsv tab-delimited file, trinity
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	18 and creates a tabular report
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	19
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	20 Would it be possible to create 2 additional files from the deFuse-Trinity comparison program.
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	21 One containing all the Trinity records matched to deFuse records (with the deFuse ID number),
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	22 and the other with the ORFs records matching back to the Trinity records in the first files?
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	23
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	24 M045_Report.csv
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	25 "","deFuse_subset.count","deFuse.gene_name1","deFuse.gene_name2","deFuse.span_count","deFuse.probability","deFuse.gene_chromosome1","deFuse.gene_location1","deFuse.gene_chromosome2","deFuse.gene_location2","deFuse_subset.type"
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	26 "1",1,"Rps6","Dennd4c",7,0.814853504,"4","coding","4","coding","TIC "
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	27
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	28
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	29
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	30 OS03_Matched_Rev.csv
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	31 "count","gene1","gene2","breakpoint","fusion","Trinity_transcript_ID","Trinity_transcript","ID1","protein"
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	32
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	33 "","deFuse.splitr_sequence","deFuse.gene_chromosome1","deFuse.gene_chromosome2","deFuse.gene_location1","deFuse.gene_location2","deFuse.gene_name1","deFuse.gene_name2","deFuse.span_count","deFuse.probability","word1","word2","fusion_part_1","fusion_part_2","fusion_point","fusion_point_rc","count","transcript"
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	34
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	35 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	36
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	37 import sys,re,os.path,math
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	38 import textwrap
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	39 import optparse
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	40 from optparse import OptionParser
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	41
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	42 revcompl = lambda x: ''.join([{'A':'T','C':'G','G':'C','T':'A','a':'t','c':'g','g':'c','t':'a','N':'N','n':'n'}[B] for B in x][::-1])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	43
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	44 codon_map = {"UUU":"F", "UUC":"F", "UUA":"L", "UUG":"L",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	45 "UCU":"S", "UCC":"S", "UCA":"S", "UCG":"S",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	46 "UAU":"Y", "UAC":"Y", "UAA":"", "UAG":"",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	47 "UGU":"C", "UGC":"C", "UGA":"*", "UGG":"W",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	48 "CUU":"L", "CUC":"L", "CUA":"L", "CUG":"L",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	49 "CCU":"P", "CCC":"P", "CCA":"P", "CCG":"P",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	50 "CAU":"H", "CAC":"H", "CAA":"Q", "CAG":"Q",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	51 "CGU":"R", "CGC":"R", "CGA":"R", "CGG":"R",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	52 "AUU":"I", "AUC":"I", "AUA":"I", "AUG":"M",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	53 "ACU":"T", "ACC":"T", "ACA":"T", "ACG":"T",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	54 "AAU":"N", "AAC":"N", "AAA":"K", "AAG":"K",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	55 "AGU":"S", "AGC":"S", "AGA":"R", "AGG":"R",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	56 "GUU":"V", "GUC":"V", "GUA":"V", "GUG":"V",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	57 "GCU":"A", "GCC":"A", "GCA":"A", "GCG":"A",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	58 "GAU":"D", "GAC":"D", "GAA":"E", "GAG":"E",
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	59 "GGU":"G", "GGC":"G", "GGA":"G", "GGG":"G",}
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	60
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	61 def translate(seq) :
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	62 rna = seq.upper().replace('T','U')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	63 aa = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	64 for i in range(0,len(rna) - 2, 3):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	65 codon = rna[i:i+3]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	66 aa.append(codon_map[codon] if codon in codon_map else 'X')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	67 return ''.join(aa)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	68
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	69 def get_stop_codons(seq) :
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	70 rna = seq.upper().replace('T','U')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	71 stop_codons = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	72 for i in range(0,len(rna) - 2, 3):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	73 codon = rna[i:i+3]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	74 aa = codon_map[codon] if codon in codon_map else 'X'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	75 if aa == '*':
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	76 stop_codons.append(codon)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	77 return stop_codons
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	78
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	79 def read_fasta(fp):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	80 name, seq = None, []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	81 for line in fp:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	82 line = line.rstrip()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	83 if line.startswith(">"):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	84 if name: yield (name, ''.join(seq))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	85 name, seq = line, []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	86 else:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	87 seq.append(line)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	88 if name: yield (name, ''.join(seq))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	89
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	90
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	91 def test_rcomplement(seq, target):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	92 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	93 comp = revcompl(seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	94 return comp in target
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	95 except:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	96 pass
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	97 return False
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	98
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	99 def test_reverse(seq,target):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	100 return options.test_reverse and seq and seq[::-1] in target
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	101
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	102 def cmp_alphanumeric(s1,s2):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	103 if s1 == s2:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	104 return 0
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	105 a1 = re.findall("\d+\|[a-zA-Z]+",s1)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	106 a2 = re.findall("\d+\|[a-zA-Z]+",s2)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	107 for i in range(min(len(a1),len(a2))):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	108 if a1[i] == a2[i]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	109 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	110 if a1[i].isdigit() and a2[i].isdigit():
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	111 return int(a1[i]) - int(a2[i])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	112 return 1 if a1[i] > a2[i] else -1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	113 return len(a1) - len(a2)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	114
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	115 def parse_defuse_results(inputFile):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	116 defuse_results = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	117 columns = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	118 coltype_int = ['expression1', 'expression2', 'gene_start1', 'gene_start2', 'gene_end1', 'gene_end2', 'genomic_break_pos1', 'genomic_break_pos2', 'breakpoint_homology', 'span_count', 'splitr_count', 'splice_score']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	119 coltype_float = ['probability']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	120 coltype_yn = [ 'orf', 'exonboundaries', 'read_through', 'interchromosomal', 'adjacent', 'altsplice', 'deletion', 'eversion', 'inversion']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	121 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	122 for linenum,line in enumerate(inputFile):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	123 ## print >> sys.stderr, "%d: %s\n" % (linenum,line)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	124 fields = line.strip().split('\t')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	125 if line.startswith('cluster_id'):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	126 columns = fields
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	127 ## print >> sys.stderr, "columns: %s\n" % columns
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	128 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	129 elif fields and len(fields) == len(columns):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	130 cluster_id = fields[columns.index('cluster_id')]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	131 cluster = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	132 flags = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	133 defuse_results.append(cluster)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	134 for i,v in enumerate(columns):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	135 if v in coltype_int:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	136 cluster[v] = int(fields[i])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	137 elif v in coltype_float:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	138 cluster[v] = float(fields[i])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	139 elif v in coltype_yn:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	140 cluster[v] = fields[i] == 'Y'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	141 if cluster[v]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	142 flags.append(columns[i])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	143 else:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	144 cluster[v] = fields[i]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	145 cluster['flags'] = ','.join(flags)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	146 except Exception, e:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	147 print >> sys.stderr, "failed to read cluster_dict: %s" % e
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	148 exit(1)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	149 return defuse_results
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	150
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	151 ## deFuse params to the mapping application?
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	152
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	153 def __main__():
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	154 #Parse Command Line
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	155 parser = optparse.OptionParser()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	156 # files
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	157 parser.add_option( '-i', '--input', dest='input', default=None, help='The input defuse results.tsv file (else read from stdin)' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	158 parser.add_option( '-t', '--transcripts', dest='transcripts', default=None, help='Trinity transcripts' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	159 parser.add_option( '-p', '--peptides', dest='peptides', default=None, help='Trinity ORFs' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	160 parser.add_option( '-o', '--output', dest='output', default=None, help='The output report (else write to stdout)' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	161 parser.add_option( '-m', '--matched', dest='matched', default=None, help='The output matched report' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	162 parser.add_option( '-a', '--transcript_alignment', dest='transcript_alignment', default=None, help='The output alignment file' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	163 parser.add_option( '-A', '--orf_alignment', dest='orf_alignment', default=None, help='The output ORF alignment file' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	164 parser.add_option( '-N', '--nbases', dest='nbases', type='int', default=12, help='Number of bases on either side of the fusion to compare' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	165 parser.add_option( '-L', '--min_pep_len', dest='min_pep_len', type='int', default=100, help='Minimum length of peptide to report' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	166 parser.add_option( '-T', '--ticdist', dest='ticdist', type='int', default=1000000, help='Maximum intrachromosomal distance to be classified a Transcription-induced chimera (TIC)' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	167 parser.add_option( '-P', '--prior_aa', dest='prior_aa', type='int', default=11, help='Number of protein AAs to show preceeding fusion point' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	168 parser.add_option( '-I', '--incomplete_orfs', dest='incomplete_orfs', action='store_true', default=False, help='Count incomplete ORFs' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	169 parser.add_option( '-O', '--orf_type', dest='orf_type', action='append', default=['complete','5prime_partial'], choices=['complete','5prime_partial','3prime_partial','internal'], help='ORF types to report' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	170 parser.add_option( '-r', '--readthrough', dest='readthrough', type='int', default=3, help='Number of stop_codons to read through' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	171 # min_orf_len
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	172 # split_na_len
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	173 # tic_len = 1000000
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	174 # prior
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	175 # deFuse direction reversed
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	176 # in frame ?
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	177 # contain known protein elements
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	178 # what protein change
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	179 # trinity provides full transctipt, defuse doesn't show full
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	180 #parser.add_option( '-r', '--reference', dest='reference', default=None, help='The genomic reference fasta' )
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	181 #parser.add_option( '-g', '--gtf', dest='gtf', default=None, help='The genomic reference gtf feature file')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	182 (options, args) = parser.parse_args()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	183
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	184 # results.tsv input
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	185 if options.input != None:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	186 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	187 inputPath = os.path.abspath(options.input)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	188 inputFile = open(inputPath, 'r')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	189 except Exception, e:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	190 print >> sys.stderr, "failed: %s" % e
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	191 exit(2)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	192 else:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	193 inputFile = sys.stdin
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	194 # vcf output
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	195 if options.output != None:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	196 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	197 outputPath = os.path.abspath(options.output)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	198 outputFile = open(outputPath, 'w')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	199 except Exception, e:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	200 print >> sys.stderr, "failed: %s" % e
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	201 exit(3)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	202 else:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	203 outputFile = sys.stdout
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	204 outputTxFile = None
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	205 outputOrfFile = None
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	206 if options.transcript_alignment:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	207 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	208 outputTxFile = open(options.transcript_alignment,'w')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	209 except Exception, e:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	210 print >> sys.stderr, "failed: %s" % e
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	211 exit(3)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	212 if options.orf_alignment:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	213 try:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	214 outputOrfFile = open(options.orf_alignment,'w')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	215 except Exception, e:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	216 print >> sys.stderr, "failed: %s" % e
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	217 exit(3)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	218 # Add percent match after transcript
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	219 report_fields = ['gene_name1','gene_name2','span_count','probability','gene_chromosome1','gene_location1','gene_chromosome2','gene_location2','fusion_type','Transcript','coverage','Protein','flags','alignments1','alignments2']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	220 report_fields = ['cluster_id','gene_name1','gene_name2','span_count','probability','genomic_bkpt1','gene_location1','genomic_bkpt2','gene_location2','fusion_type','Transcript','coverage','Protein','flags','alignments1','alignments2']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	221 report_colnames = {'gene_name1':'Gene 1','gene_name2':'Gene 2','span_count':'Span cnt','probability':'Probability','gene_chromosome1':'From Chr','gene_location1':'Fusion point','gene_chromosome2':'To Chr','gene_location2':'Fusion point', 'cluster_id':'cluster_id', 'splitr_sequence':'splitr_sequence', 'splitr_count':'splitr_count', 'splitr_span_pvalue':'splitr_span_pvalue', 'splitr_pos_pvalue':'splitr_pos_pvalue', 'splitr_min_pvalue':'splitr_min_pvalue', 'adjacent':'adjacent', 'altsplice':'altsplice', 'break_adj_entropy1':'break_adj_entropy1', 'break_adj_entropy2':'break_adj_entropy2', 'break_adj_entropy_min':'break_adj_entropy_min', 'breakpoint_homology':'breakpoint_homology', 'breakseqs_estislands_percident':'breakseqs_estislands_percident', 'cdna_breakseqs_percident':'cdna_breakseqs_percident', 'deletion':'deletion', 'est_breakseqs_percident':'est_breakseqs_percident', 'eversion':'eversion', 'exonboundaries':'exonboundaries', 'expression1':'expression1', 'expression2':'expression2', 'gene1':'gene1', 'gene2':'gene2', 'gene_align_strand1':'gene_align_strand1', 'gene_align_strand2':'gene_align_strand2', 'gene_end1':'gene_end1', 'gene_end2':'gene_end2', 'gene_start1':'gene_start1', 'gene_start2':'gene_start2', 'gene_strand1':'gene_strand1', 'gene_strand2':'gene_strand2', 'genome_breakseqs_percident':'genome_breakseqs_percident', 'genomic_break_pos1':'genomic_break_pos1', 'genomic_break_pos2':'genomic_break_pos2', 'genomic_strand1':'genomic_strand1', 'genomic_strand2':'genomic_strand2', 'interchromosomal':'interchromosomal', 'interrupted_index1':'interrupted_index1', 'interrupted_index2':'interrupted_index2', 'inversion':'inversion', 'library_name':'library_name', 'max_map_count':'max_map_count', 'max_repeat_proportion':'max_repeat_proportion', 'mean_map_count':'mean_map_count', 'min_map_count':'min_map_count', 'num_multi_map':'num_multi_map', 'num_splice_variants':'num_splice_variants', 'orf':'orf', 'read_through':'read_through', 'repeat_proportion1':'repeat_proportion1', 'repeat_proportion2':'repeat_proportion2', 'span_coverage1':'span_coverage1', 'span_coverage2':'span_coverage2', 'span_coverage_max':'span_coverage_max', 'span_coverage_min':'span_coverage_min', 'splice_score':'splice_score', 'splicing_index1':'splicing_index1', 'splicing_index2':'splicing_index2', 'fusion_type':'Type', 'coverage':'fusion%','Transcript':'Transcript?','Protein':'Protein?','flags':'descriptions','fwd_seq':'fusion','alignments1':'alignments1','alignments2':'alignments2','genomic_bkpt1':'From Chr', 'genomic_bkpt2':'To Chr'}
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	222
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	223 ## Read defuse results
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	224 fusions = parse_defuse_results(inputFile)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	225 ## Create a field with the 12 nt before and after the fusion point.
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	226 ## Create a field with the reverse complement of the 24 nt fusion point field.
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	227 ## Add fusion type filed (INTER, INTRA, TIC)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	228 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	229 fusion['ordinal'] = i + 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	230 fusion['genomic_bkpt1'] = "%s:%d" % (fusion['gene_chromosome1'], fusion['genomic_break_pos1'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	231 fusion['genomic_bkpt2'] = "%s:%d" % (fusion['gene_chromosome2'], fusion['genomic_break_pos2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	232 fusion['alignments1'] = "%s%s%s" % (fusion['genomic_strand1'], fusion['gene_strand1'], fusion['gene_align_strand1'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	233 fusion['alignments2'] = "%s%s%s" % (fusion['genomic_strand2'], fusion['gene_strand2'], fusion['gene_align_strand2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	234 split_seqs = fusion['splitr_sequence'].split('\|')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	235 fusion['split_seqs'] = split_seqs
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	236 fusion['split_seqs'] = split_seqs
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	237 fusion['split_seq_lens'] = [len(split_seqs[0]),len(split_seqs[1])]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	238 fusion['split_max_lens'] = [len(split_seqs[0]),len(split_seqs[1])]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	239 fwd_off = min(abs(options.nbases),len(split_seqs[0]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	240 rev_off = min(abs(options.nbases),len(split_seqs[1]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	241 fusion['fwd_off'] = fwd_off
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	242 fusion['rev_off'] = rev_off
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	243 fwd_seq = split_seqs[0][-fwd_off:] + split_seqs[1][:rev_off]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	244 rev_seq = revcompl(fwd_seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	245 fusion['fwd_seq'] = fwd_seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	246 fusion['rev_seq'] = rev_seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	247 fusion_type = 'inter' if fusion['gene_chromosome1'] != fusion['gene_chromosome2'] else 'intra' if abs(fusion['genomic_break_pos1'] - fusion['genomic_break_pos2']) > options.ticdist else 'TIC'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	248 fusion['fusion_type'] = fusion_type
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	249 fusion['transcripts'] = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	250 fusion['Transcript'] = 'No'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	251 fusion['coverage'] = 0
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	252 fusion['Protein'] = 'No'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	253 # print >> sys.stdout, "%4d\t%6s\t%s\t%s\t%s\t%s\t%s" % (i,fusion['cluster_id'],fwd_seq,rev_seq,fusion_type,fusion['gene_name1'],fusion['gene_name2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	254 inputFile.close()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	255
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	256 ## Process Trinity data and compare to deFuse
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	257 matched_transcripts = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	258 matched_orfs = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	259 transcript_orfs = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	260 fusions_with_transcripts = set()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	261 fusions_with_orfs = set()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	262 ## fusion['transcripts'][tx_id] { revcompl:?, bkpt:n, seq1: , seq2: , match1:n, match2:n}
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	263 n = 0
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	264 if options.transcripts:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	265 with open(options.transcripts) as fp:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	266 for tx_full_id, seq in read_fasta(fp):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	267 n += 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	268 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	269 if fusion['fwd_seq'] in seq or fusion['rev_seq'] in seq:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	270 fusions_with_transcripts.add(i)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	271 fusion['Transcript'] = 'Yes'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	272 tx_id = tx_full_id.lstrip('>').split()[0]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	273 matched_transcripts[tx_full_id] = seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	274 fusion['transcripts'][tx_id] = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	275 fusion['transcripts'][tx_id]['seq'] = seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	276 fusion['transcripts'][tx_id]['full_id'] = tx_full_id
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	277 pos = seq.find(fusion['fwd_seq'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	278 if pos >= 0:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	279 tx_bkpt = pos + fusion['fwd_off']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	280 # fusion['transcripts'][tx_full_id] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	281 if tx_bkpt > fusion['split_max_lens'][0]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	282 fusion['split_max_lens'][0] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	283 len2 = len(seq) - tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	284 if len2 > fusion['split_max_lens'][1]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	285 fusion['split_max_lens'][1] = len2
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	286 fusion['transcripts'][tx_id]['bkpt'] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	287 fusion['transcripts'][tx_id]['revcompl'] = False
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	288 fusion['transcripts'][tx_id]['seq1'] = seq[:tx_bkpt]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	289 fusion['transcripts'][tx_id]['seq2'] = seq[tx_bkpt:]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	290 else:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	291 pos = seq.find(fusion['rev_seq'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	292 tx_bkpt = pos + fusion['rev_off']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	293 # fusion['transcripts'][tx_full_id] = -tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	294 if tx_bkpt > fusion['split_max_lens'][1]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	295 fusion['split_max_lens'][1] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	296 len2 = len(seq) - tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	297 if len2 > fusion['split_max_lens'][0]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	298 fusion['split_max_lens'][0] = len2
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	299 rseq = revcompl(seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	300 pos = rseq.find(fusion['fwd_seq'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	301 tx_bkpt = pos + fusion['fwd_off']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	302 fusion['transcripts'][tx_id]['bkpt'] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	303 fusion['transcripts'][tx_id]['revcompl'] = True
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	304 fusion['transcripts'][tx_id]['seq1'] = rseq[:tx_bkpt]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	305 fusion['transcripts'][tx_id]['seq2'] = rseq[tx_bkpt:]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	306 fseq = fusion['split_seqs'][0]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	307 tseq = fusion['transcripts'][tx_id]['seq1']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	308 mlen = min(len(fseq),len(tseq))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	309 fusion['transcripts'][tx_id]['match1'] = mlen
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	310 for j in range(1,mlen+1):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	311 if fseq[-j] != tseq[-j]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	312 fusion['transcripts'][tx_id]['match1'] = j - 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	313 break
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	314 fseq = fusion['split_seqs'][1]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	315 tseq = fusion['transcripts'][tx_id]['seq2']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	316 mlen = min(len(fseq),len(tseq))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	317 fusion['transcripts'][tx_id]['match2'] = mlen
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	318 for j in range(mlen):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	319 if fseq[j] != tseq[j]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	320 fusion['transcripts'][tx_id]['match2'] = j
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	321 break
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	322 # coverage = math.floor(float(fusion['transcripts'][tx_id]['match1'] + fusion['transcripts'][tx_id]['match2']) * 100. / len(fusion['split_seqs'][0]+fusion['split_seqs'][1]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	323 coverage = int((fusion['transcripts'][tx_id]['match1'] + fusion['transcripts'][tx_id]['match2']) * 1000. / len(fusion['split_seqs'][0]+fusion['split_seqs'][1])) * .1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	324 # print >> sys.stderr, "%s\t%d\t%d\t%d\%s\t\t%d\t%d\t%d\t%d" % (tx_id,fusion['transcripts'][tx_id]['match1'],fusion['transcripts'][tx_id]['match2'],len(fusion['split_seqs'][0]+fusion['split_seqs'][1]),coverage,len( fusion['split_seqs'][0]),len(fusion['transcripts'][tx_id]['seq1']),len(fusion['split_seqs'][1]),len(fusion['transcripts'][tx_id]['seq2']))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	325 fusion['coverage'] = max(coverage,fusion['coverage'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	326 print >> sys.stdout, "fusions_with_transcripts: %d %s\n matched_transcripts: %d" % (len(fusions_with_transcripts),fusions_with_transcripts,len(matched_transcripts))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	327 ##for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	328 ## print >> sys.stdout, "%4d\t%6s\t%s\t%s\t%s\t%s\t%s\t%s" % (i,fusion['cluster_id'],fusion['fwd_seq'],fusion['rev_seq'],fusion['fusion_type'],fusion['gene_name1'],fusion['gene_name2'], fusion['transcripts'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	329 ## Process ORFs and compare to matched deFuse and Trinity data.
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	330 ## Proteins must be at least 100 aa long, starting at the first "M" and must end with an "*".
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	331 if options.peptides:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	332 with open(options.peptides) as fp:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	333 for orf_full_id, seq in read_fasta(fp):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	334 n += 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	335 if len(seq) < options.min_pep_len:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	336 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	337 orf_type = re.match('^.* type:(\S+) .*$',orf_full_id).groups()[0]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	338 ## if not seq[-1] == '*' and not options.incomplete_orfs:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	339 ## if not orf_type 'complete' and not options.incomplete_orfs:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	340 if orf_type not in options.orf_type:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	341 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	342 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	343 if len(fusion['transcripts']) > 0:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	344 for tx_id in fusion['transcripts']:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	345 ## >m.196252 g.196252 ORF g.196252 m.196252 type:complete len:237 (+) comp100000_c5_seq2:315-1025(+)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	346 ## >m.134565 g.134565 ORF g.134565 m.134565 type:5prime_partial len:126 (-) comp98702_c1_seq21:52-429(-)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	347 if tx_id+':' not in orf_full_id:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	348 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	349 m = re.match("^.%s:(\d+)-(\d+)[(]([+-])[)]." % re.sub('([\|.{}()$?^])','[\\1]',tx_id),orf_full_id)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	350 if m:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	351 if not m.groups() or len(m.groups()) < 3 or m.groups()[0] == None:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	352 print >> sys.stderr, "Error:\n%s\n%s\n" % (tx_id,orf_full_id)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	353 orf_id = orf_full_id.lstrip('>').split()[0]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	354 if not tx_id in transcript_orfs:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	355 transcript_orfs[tx_id] = []
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	356 alignments = "%s%s%s %s%s%s" % (fusion['genomic_strand1'], fusion['gene_strand1'], fusion['gene_align_strand1'], fusion['genomic_strand2'], fusion['gene_strand2'], fusion['gene_align_strand2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	357 # print >> sys.stdout, "%d %s bkpt:%d %s rc:%s (%s) %s" % (fusion['ordinal'], tx_id, int(fusion['transcripts'][tx_id]['bkpt']), str(m.groups()), str(fusion['transcripts'][tx_id]['revcompl']), alignments, orf_full_id)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	358 start = seq.find('M')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	359 pep_len = len(seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	360 if pep_len - start < options.min_pep_len:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	361 continue
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	362 orf_dict = dict()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	363 transcript_orfs[tx_id].append(orf_dict)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	364 fusions_with_orfs.add(i)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	365 matched_orfs[orf_full_id] = seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	366 fusion['Protein'] = 'Yes'
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	367 tx_start = int(m.groups()[0])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	368 tx_end = int(m.groups()[1])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	369 tx_strand = m.groups()[2]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	370 tx_bkpt = fusion['transcripts'][tx_id]['bkpt']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	371 orf_dict['orf_id'] = orf_id
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	372 orf_dict['tx_start'] = tx_start
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	373 orf_dict['tx_end'] = tx_end
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	374 orf_dict['tx_strand'] = tx_strand
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	375 orf_dict['tx_bkpt'] = tx_bkpt
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	376 orf_dict['seq'] = seq[:start].lower() + seq[start:] if start > 0 else seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	377 ## >m.208656 g.208656 ORF g.208656 m.208656 type:5prime_partial len:303 (+) comp100185_c2_seq9:2-910(+)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	378 ## translate(tx34[1:910])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	379 ## translate(tx34[1:2048])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	380 ## comp99273_c1_seq1 len=3146 (-2772)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	381 ## >m.158338 g.158338 ORF g.158338 m.158338 type:complete len:785 (-) comp99273_c1_seq1:404-2758(-)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	382 ## translate(tx[-2758:-403])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	383 ## comp100185_c2_seq9 len=2048 (904)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	384 ## novel protein sequence
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	385 ## find first novel AA
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	386 ## get prior n AAs
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	387 ## get novel AA seq thru n stop codons
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	388 ### tx_seq = matched_transcripts[tx_full_id] if tx_bkpt >= 0 else revcompl(tx_seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	389 tx_seq = fusion['transcripts'][tx_id]['seq']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	390 orf_dict['tx_seq'] = tx_seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	391 novel_tx_seq = tx_seq[tx_start - 1:] if tx_strand == '+' else revcompl(tx_seq[:tx_end])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	392 read_thru_pep = translate(novel_tx_seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	393 # fusion['transcripts'][tx_id]['revcompl'] = True
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	394 # tx_bkpt = fusion['transcripts'][tx_id]['bkpt']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	395 # bkpt_aa_pos = tx_bkpt - tx_start - 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	396 # bkpt_aa_pos = (tx_bkpt - tx_start - 1) / 3 if tx_strand == '+' else tx_end
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	397 # print >> sys.stdout, "%s\n%s" % (seq,read_thru_pep)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	398 stop_codons = get_stop_codons(novel_tx_seq)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	399 if options.readthrough:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	400 readthrough = options.readthrough + 1
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	401 read_thru_pep = ''.join(read_thru_pep.split('')[:readthrough])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	402 stop_codons = stop_codons[:readthrough]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	403 orf_dict['read_thru_pep'] = read_thru_pep
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	404 orf_dict['stop_codons'] = ','.join(stop_codons)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	405 print >> sys.stdout, "fusions_with_orfs: %d %s\n matched_orfs: %d" % (len(fusions_with_orfs),fusions_with_orfs,len(matched_orfs))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	406 ## Alignments 3 columns, seq columns padded out to longest seq, UPPERCASE_match diffs lowercase
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	407 ### defuse_id pre_split_seq post_split_seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	408 ### trinity_id pre_split_seq post_split_seq
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	409 ## Transcripts alignment output
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	410 ## Peptide alignment output
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	411 ## Write reports
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	412 ## OS03_Matched_Rev.csv
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	413 ## "count","gene1","gene2","breakpoint","fusion","Trinity_transcript_ID","Trinity_transcript","ID1","protein"
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	414 if options.transcripts and options.matched:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	415 #match_fields = ['ordinal','gene_name1','gene_name2','fwd_seq']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	416 outputMatchFile = open(options.matched,'w')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	417 #print >> outputMatchFile, '\t'.join(["#fusion_id","cluster_id","gene1","gene2","breakpoint","fusion","Trinity_transcript_ID","Trinity_transcript","Trinity_ORF_Transcript","Trinity_ORF_ID","protein","read_through","stop_codons"])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	418 print >> outputMatchFile, '\t'.join(["#fusion_id","cluster_id","gene1","gene2","breakpoint","fusion","Trinity_transcript_ID","Trinity_transcript","Trinity_ORF_Transcript","Trinity_ORF_ID","protein","stop_codons"])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	419 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	420 if len(fusion['transcripts']) > 0:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	421 for tx_id in fusion['transcripts'].keys():
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	422 if tx_id in transcript_orfs:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	423 for orf_dict in transcript_orfs[tx_id]:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	424 if 'tx_seq' not in orf_dict:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	425 print >> sys.stderr, "orf_dict %s" % orf_dict
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	426 #fields = [str(fusion['ordinal']),str(fusion['cluster_id']),fusion['gene_name1'],fusion['gene_name2'],fusion['fwd_seq'],fusion['splitr_sequence'],tx_id, fusion['transcripts'][tx_id]['seq1']+'\|'+fusion['transcripts'][tx_id]['seq2'],orf_dict['tx_seq'],orf_dict['orf_id'],orf_dict['seq'],orf_dict['read_thru_pep'],orf_dict['stop_codons']]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	427 fields = [str(fusion['ordinal']),str(fusion['cluster_id']),fusion['gene_name1'],fusion['gene_name2'],fusion['fwd_seq'],fusion['splitr_sequence'],tx_id, fusion['transcripts'][tx_id]['seq1']+'\|'+fusion['transcripts'][tx_id]['seq2'],orf_dict['tx_seq'],orf_dict['orf_id'],orf_dict['read_thru_pep'],orf_dict['stop_codons']]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	428 print >> outputMatchFile, '\t'.join(fields)
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	429 outputMatchFile.close()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	430 if options.transcripts and options.transcript_alignment:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	431 if outputTxFile:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	432 id_fields = ['gene_name1','alignments1','gene_name2','alignments2','span_count','probability','gene_chromosome1','gene_location1','gene_chromosome2','gene_location2','fusion_type','Transcript','Protein','flags']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	433 fa_width = 80
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	434 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	435 if len(fusion['transcripts']) > 0:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	436 alignments1 = "%s%s%s" % (fusion['genomic_strand1'], fusion['gene_strand1'], fusion['gene_align_strand1'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	437 alignments2 = "%s%s%s" % (fusion['genomic_strand2'], fusion['gene_strand2'], fusion['gene_align_strand2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	438 alignments = "%s%s%s %s%s%s" % (fusion['genomic_strand1'], fusion['gene_strand1'], fusion['gene_align_strand1'], fusion['genomic_strand2'], fusion['gene_strand2'], fusion['gene_align_strand2'])
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	439 fusion_id = "%s (%s) %s" % (i + 1,alignments,' '.join([str(fusion[x]) for x in report_fields]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	440 for tx_id in fusion['transcripts'].keys():
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	441 m1 = fusion['transcripts'][tx_id]['match1']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	442 f_seq1 = fusion['split_seqs'][0][:-m1].lower() + fusion['split_seqs'][0][-m1:]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	443 t_seq1 = fusion['transcripts'][tx_id]['seq1'][:-m1].lower() + fusion['transcripts'][tx_id]['seq1'][-m1:]
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	444 if len(f_seq1) > len(t_seq1):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	445 t_seq1 = t_seq1.rjust(len(f_seq1),'.')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	446 elif len(f_seq1) < len(t_seq1):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	447 f_seq1 = f_seq1.rjust(len(t_seq1),'.')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	448 m2 = fusion['transcripts'][tx_id]['match2']
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	449 f_seq2 = fusion['split_seqs'][1][:m2] + fusion['split_seqs'][1][m2:].lower()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	450 t_seq2 = fusion['transcripts'][tx_id]['seq2'][:m2] + fusion['transcripts'][tx_id]['seq2'][m2:].lower()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	451 if len(f_seq2) > len(t_seq2):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	452 t_seq2 = t_seq2.ljust(len(f_seq2),'.')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	453 elif len(f_seq2) < len(t_seq2):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	454 f_seq2 = f_seq2.ljust(len(t_seq2),'.')
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	455 print >> outputTxFile, ">%s\n%s\n%s" % (fusion_id,'\n'.join(textwrap.wrap(f_seq1,fa_width)),'\n'.join(textwrap.wrap(f_seq2,fa_width)))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	456 print >> outputTxFile, "%s bkpt:%d rev_compl:%s\n%s\n%s" % (fusion['transcripts'][tx_id]['full_id'],fusion['transcripts'][tx_id]['bkpt'],str(fusion['transcripts'][tx_id]['revcompl']),'\n'.join(textwrap.wrap(t_seq1,fa_width)),'\n'.join(textwrap.wrap(t_seq2,fa_width)))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	457 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	458 if options.peptides and options.orf_alignment:
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	459 pass
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	460 """
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	461 print >> outputFile,"%s\t%s" % ('#','\t'.join([report_colnames[x] for x in report_fields]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	462 for i,fusion in enumerate(fusions):
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	463 print >> outputFile,"%s\t%s" % (i + 1,'\t'.join([str(fusion[x]) for x in report_fields]))
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	464
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	465 if __name__ == "__main__" : __main__()
b22f8634ff84 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit 23b94b5747c6956360cd2eca0a07a669929ea141-dirty jjohnson parents: diff changeset	466

Mercurial > repos > jjohnson > defuse

annotate defuse_trinity_analysis.py @ 13:3a4876d01c7e draft