Mercurial > repos > jjohnson > defuse
diff defuse_trinity_analysis.xml @ 17:c3167ccca38c draft default tip
planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/defuse commit d2317dff5a89016f18038b97d057f47d949e7808-dirty
author | jjohnson |
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date | Sat, 26 Jan 2019 12:53:08 -0500 |
parents | bdd93719cede |
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--- a/defuse_trinity_analysis.xml Mon Dec 18 09:31:31 2017 -0500 +++ b/defuse_trinity_analysis.xml Sat Jan 26 12:53:08 2019 -0500 @@ -40,19 +40,21 @@ <data name="output" metadata_source="defuse_results" format="tabular" label="${tool.name} on ${on_string}: Fusion Report"/> </outputs> <tests> - <test> - <param name="defuse_results" value="mm10_results.filtered.tsv" ftype="defuse.results.tsv" dbkey="mm10"/> - <output name="vcf" file="mm10_results.filtered.vcf"/> - </test> </tests> <help> **Defuse Results** Verifies DeFuse_ fusion predictions in results.tsv with TrinityRNAseq_ assembled transcripts and ORFs. -This program relies on the header line of the results.tsv to determine which columns to use for analysis. +DeFuse provides a total fusion sequence of 200-500 nucleotides (nts) around the fusion breakpoint. This may be insufficient to predict the effect of the fusion on protein production. To get a view of the full transcript containing the fusion, Trinity de novo transcripts from the RNA-seq data are compared with the deFuse fusion sequences using a subsequence around the deFuse indetified fusion breakpoint. The Trinity transcriptToOrfs output provides potential proteins from the projected fusion transcript. + +This program relies on the header line of the deFuse results.tsv to determine which columns to use for analysis. .. _DeFuse: http://sourceforge.net/apps/mediawiki/defuse .. _TrinityRNAseq: http://trinityrnaseq.github.io/ </help> + <expand macro="citations"> + <citation type="doi">10.1038/nbt.1883</citation> + <citation type="doi">10.1038/s41598-018-36840-z</citation> + </expand> </tool>