Mercurial > repos > jjohnson > encyclopedia_walnut
diff encyclopedia_walnut.xml @ 5:4936de9f9024 draft
"planemo upload for repository https://github.com/galaxyproteomics/tools-galaxyp/tree/encyclopedia/tools/encyclopedia commit 880d327a6dce00616a0b0b58bcabcea7b58358cc"
author | jjohnson |
---|---|
date | Wed, 19 Aug 2020 08:39:11 -0400 |
parents | 2f36eba0b877 |
children | 0172dfa08216 |
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--- a/encyclopedia_walnut.xml Tue Jul 07 11:56:49 2020 -0400 +++ b/encyclopedia_walnut.xml Wed Aug 19 08:39:11 2020 -0400 @@ -61,52 +61,43 @@ </actions> </data> </outputs> + <tests> + <test> + <param name="scan_input" ftype="mzml" value="BCS_hela_narrow_3_1.mzML"/> + <param name="fasta" ftype="fasta" value="uniprot_tiny_human.fasta"/> + <output name="features" ftype="tabular"> + <assert_contents> + <has_text text="LHYNEGLNIK"/> + </assert_contents> + </output> + </test> + </tests> <help><![CDATA[ + + <help><![CDATA[ + +**Walnut** + +@ENCYCLOPEDIA_WIKI@ + Walnut is a FASTA database search engine for Data-Independent Acquisition (DIA) MS/MS data. Walnut uses PeCAn-style scoring to extract peptide fragmentation chromatograms from MZML files, assign peaks, and calculate various peak features. These features are interpreted by Percolator to identify peptides. -Required Parameters: - -i input .DIA or .MZML file - -f background FASTA file + +**Inputs** + + + - A spectrum file in mzML format + - A protein data base in fasta format + -Other Parameters: - -t target FASTA file (default: background FASTA file) - -tp true/false target FASTA file contains peptides (default: false) - -o output report file (default: [input file].pecan.txt) - -acquisition (default: overlapping dia) - -addDecoysToBackground (default: false) - -alpha (default: 1.8) - -beta (default: 0.4) - -dontRunDecoys (default: false) - -enzyme (default: trypsin) - -filterPeaklists (default: false) - -fixed (default: C=57.0214635) - -foffset (default: 0) - -frag (default: YONLY) - -ftol (default: 10) - -ftolunits (default: ppm) - -maxCharge (default: 3) - -maxLength (default: 100) - -maxMissedCleavage (default: 1) - -minCharge (default: 2) - -minEluteTime (default: 12) - -minIntensity (default: -1.0) - -minLength (default: 5) - -minNumOfQuantitativePeaks (default: 3) - -minQuantitativeIonNumber (default: 3) - -numberOfQuantitativePeaks (default: 5) - -numberOfReportedPeaks (default: 1) - -numberOfThreadsUsed (default: 12) - -percolatorProteinThreshold (default: 0.01) - -percolatorThreshold (default: 0.01) - -percolatorVersionNumber (default: 3) - -poffset (default: 0) - -precursorIsolationMargin (default: 0) - -precursorWindowSize (default: -1) - -ptol (default: 10) - -ptolunits (default: ppm) - -requireVariableMods (default: false) - -variable (default: -) +@MSCONVERT_HELP@ + +**Outputs** + + - A log file + - The identified features in tabular format + - The identified proteins in tabular format ]]></help> <expand macro="citations" />