Mercurial > repos > jjohnson > ensembl_variant_report

annotate ensembl_variant_report.py @ 4:7fc91849ab21 draft default tip

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"planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit 4078a495c5569e9055b4eba33004fe609ee42aff"
author jjohnson
date Sat, 25 Jan 2020 15:22:46 -0500
parents 652d35c42bca
children
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9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
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1 #!/usr/bin/env python
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2 """
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3 Report variants to Ensembl Transcripts
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4
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5 FrameShift report line
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6 # Gene Variant Position Reference Variation Prevalence Sequencing Depth Transcript AA Position AA change AA Length Stop Codon Stop Region AA Variation
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7 TIGD6 chr5:149374879 AAG AG 1.00 13 ENSG00000164296|ENST00000296736 345 Q344 522 A-TGA-T KRWTSSRPST*
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8
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9 MissSense report line
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10 # Gene Variant Position Reference Variation Prevalence Sequencing Depth Transcript AA Position AA change AA Length Stop Codon Stop Region AA Variation
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11 FN1 chr2:216235089 G A 1.00 7394 ENSG00000115414|ENST00000354785 2261 V2261I 2478 G-TAA TGLTRGATYN_I_IVEALKDQQR
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12 """
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13 import sys
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14 import os.path
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15 import re
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16 import time
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17 import optparse
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18 from ensemblref import EnsemblRef
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19 from Bio.Seq import reverse_complement, translate
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20
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21
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22 def __main__():
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23 #Parse Command Line
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24 parser = optparse.OptionParser()
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25 #I/O
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26 parser.add_option( '-i', '--input', dest='input', default=None, help='Tabular file with peptide_sequence column' )
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27 parser.add_option( '-s', '--format', dest='format', default='tabular', choices=['tabular','snpeff'], help='Tabular file with peptide_sequence column' )
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28 #Columns for tabular input
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29 parser.add_option( '-C', '--chrom_column', type='int', dest='chrom_column', default=1, help='column ordinal with Ensembl transctip ID' )
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30 parser.add_option( '-P', '--pos_column', type='int', dest='pos_column', default=2, help='column ordinal with Ensembl transctip ID' )
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31 parser.add_option( '-R', '--ref_column', type='int', dest='ref_column', default=3, help='column ordinal with Ensembl transctip ID' )
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32 parser.add_option( '-A', '--alt_column', type='int', dest='alt_column', default=4, help='column ordinal with Ensembl transctip ID' )
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33 parser.add_option( '-T', '--transcript_column', type='int', dest='transcript_column', default=1, help='column ordinal with Ensembl transctip ID' )
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34 parser.add_option( '-F', '--dpr_column', type='int', dest='dpr_column', default=1, help='column with VCF: DPR or AD' )
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35 parser.add_option( '-D', '--dp_column', type='int', dest='dp_column', default=1, help='column with VCF: DP' )
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36 parser.add_option( '-g', '--gene_model', dest='gene_model', default=None, help='GTF gene model file. Used to annotate NSJ peptide entries.')
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37 parser.add_option( '-2', '--twobit', dest='twobit', default=None, help='Reference genome in UCSC twobit format')
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38 #Output file
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39 parser.add_option( '-o', '--output', dest='output', default=None, help='The output report (else write to stdout)' )
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40 #filters
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41 parser.add_option( '-d', '--min_depth', type='int', dest='min_depth', default=None, help='Minimum read depth to report' )
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42 parser.add_option( '-f', '--min_freq', type='float', dest='min_freq', default=None, help='Minimum variant frequency to report' )
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43 #peptide options
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44 parser.add_option( '-l', '--leading_aa', type='int', dest='leading_aa', default=10, help='Number AAs before missense variant' )
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45 parser.add_option( '-t', '--trailing_aa', type='int', dest='trailing_aa', default=10, help='Number AAs after missense variant' )
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46 parser.add_option( '-r', '--readthrough', type='int', dest='readthrough', default=0, help='' )
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47 #
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48 parser.add_option('--debug', dest='debug', action='store_true', default=False, help='Print debugging messages')
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49 (options, args) = parser.parse_args()
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50
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51 ##INPUTS##
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52 if options.input != None:
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53 try:
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54 inputPath = os.path.abspath(options.input)
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55 inputFile = open(inputPath, 'r')
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56 except Exception, e:
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57 print >> sys.stderr, "failed: %s" % e
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58 exit(2)
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59 else:
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60 inputFile = sys.stdin
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61
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62 if options.output != None:
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63 try:
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64 outputPath = os.path.abspath(options.output)
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65 outputFile = open(outputPath, 'w')
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66 except Exception, e:
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67 print >> sys.stderr, "failed: %s" % e
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68 exit(3)
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69 else:
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70 outputFile = sys.stdout
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71
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72 def parse_tabular():
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73 ci = options.chrom_column - 1
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74 pi = options.pos_column - 1
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75 ri = options.ref_column - 1
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76 ai = options.alt_column - 1
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77 ti = options.transcript_column - 1
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78 di = options.dp_column - 1
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79 fi = options.dpr_column - 1
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80 for linenum,line in enumerate(inputFile):
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81 if options.debug:
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82 print >> sys.stderr, "%d: %s\n" % (linenum,line)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
83 if line.startswith('#'):
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
84 continue
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
85 if line.strip() == '':
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
86 continue
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jjohnson
parents:
diff changeset
87 fields = line.rstrip('\r\n').split('\t')
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
88 transcript = fields[ti]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
89 if not transcript:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
90 print >> sys.stderr, "%d: %s\n" % (linenum,line)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
91 continue
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
92 chrom = fields[ci]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
93 pos = int(fields[pi])
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
94 ref = fields[ri]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
95 alts = fields[ai]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
96 dp = int(fields[di])
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
97 dpr = [int(x) for x in fields[fi].split(',')]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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diff changeset
98 for i,alt in enumerate(alts.split(',')):
2
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jjohnson
parents: 1
diff changeset
99 freq = float(dpr[i+1])/float(dp) if dp and dpr else \
f87fe6bc48f4 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit 6b6e5c13531bf909c4c70b7f8f9e28b4206d9068-dirty
jjohnson
parents: 1
diff changeset
100 float(dpr[i+1])/float(sum(dpr)) if dpr else None
0
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
101 yield (transcript,pos,ref,alt,dp,freq)
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jjohnson
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102
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jjohnson
parents:
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103 def parse_snpeff_vcf():
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jjohnson
parents:
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104 for linenum,line in enumerate(inputFile):
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
105 if line.startswith('##'):
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
106 if line.find('SnpEffVersion=') > 0:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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107 SnpEffVersion = re.search('SnpEffVersion="?(\d+\.\d+)',line).groups()[0]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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108 elif line.startswith('#CHROM'):
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jjohnson
parents:
diff changeset
109 pass
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jjohnson
parents:
diff changeset
110 else:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
111 fields = line.strip('\r\n').split('\t')
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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112 if options.debug: print >> sys.stderr, "\n%s" % (fields)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
113 (chrom, pos, id, ref, alts, qual, filter, info) = fields[0:8]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
114 alt_list = alts.split(',')
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
115 pos = int(pos)
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jjohnson
parents:
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116 qual = float(qual)
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jjohnson
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diff changeset
117 dp = None
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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118 dpr = None
3
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jjohnson
parents: 2
diff changeset
119 ao = None
2
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jjohnson
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diff changeset
120 af = None
0
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jjohnson
parents:
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121 for info_item in info.split(';'):
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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122 if info_item.find('=') < 0: continue
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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123 (key, val) = info_item.split('=', 1)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
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124 if key == 'DP':
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jjohnson
parents:
diff changeset
125 dp = int(val)
2
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jjohnson
parents: 1
diff changeset
126 if key == 'DPR' or key == 'AD':
0
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jjohnson
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127 dpr = [int(x) for x in val.split(',')]
3
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
128 if key == 'AO':
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jjohnson
parents: 2
diff changeset
129 ao = [int(x) for x in val.split(',')]
2
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jjohnson
parents: 1
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130 if key == 'AF':
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jjohnson
parents: 1
diff changeset
131 af = [float(x) for x in val.split(',')]
0
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jjohnson
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132 if key in ['EFF','ANN']:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
133 for effect in val.split(','):
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jjohnson
parents:
diff changeset
134 if options.debug: print >> sys.stderr, "\n%s" % (effect.split('|'))
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
135 if key == 'ANN':
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jjohnson
parents:
diff changeset
136 (alt,eff,impact,gene_name,gene_id,feature_type,transcript,biotype,exon,c_hgvs,p_hgvs,cdna,cds,aa,distance,info) = effect.split('|')
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
137 elif key == 'EFF':
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jjohnson
parents:
diff changeset
138 (eff, effs) = effect.rstrip(')').split('(')
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
139 (impact, functional_class, codon_change, aa_change, aa_len, gene_name, biotype, coding, transcript, exon, alt) = effs.split('|')[0:11]
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
140 i = alt_list.index(alt) if alt in alt_list else 0
3
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
141 if ao:
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
142 freq = float(ao[i])/float(dp) if dp else \
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
143 float(dpr[i])/float(sum(ao))
2
f87fe6bc48f4 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit 6b6e5c13531bf909c4c70b7f8f9e28b4206d9068-dirty
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parents: 1
diff changeset
144 elif dpr:
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jjohnson
parents: 1
diff changeset
145 freq = float(dpr[i+1])/float(dp) if dp else \
f87fe6bc48f4 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit 6b6e5c13531bf909c4c70b7f8f9e28b4206d9068-dirty
jjohnson
parents: 1
diff changeset
146 float(dpr[i+1])/float(sum(dpr))
3
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
147 elif af:
652d35c42bca planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit d3b7ff1c2f0a1eed7a65af7208e987a35627402c-dirty
jjohnson
parents: 2
diff changeset
148 freq = af[i]
2
f87fe6bc48f4 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit 6b6e5c13531bf909c4c70b7f8f9e28b4206d9068-dirty
jjohnson
parents: 1
diff changeset
149 else:
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jjohnson
parents: 1
diff changeset
150 freq = None
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jjohnson
parents: 1
diff changeset
151 if freq:
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jjohnson
parents: 1
diff changeset
152 yield (transcript,pos,ref,alt,dp,freq)
0
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jjohnson
parents:
diff changeset
153
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
154
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jjohnson
parents:
diff changeset
155 #Process gene model
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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diff changeset
156 ens_ref = None
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jjohnson
parents:
diff changeset
157 if options.gene_model != None:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
158 try:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
159 geneModelFile = os.path.abspath(options.gene_model)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
160 twoBitFile = os.path.abspath(options.twobit)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
161 print >> sys.stderr, "Parsing ensembl ref: %s %s" % (options.gene_model,options.twobit)
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jjohnson
parents:
diff changeset
162 time1 = time.time()
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jjohnson
parents:
diff changeset
163 ens_ref = EnsemblRef(geneModelFile,twoBitFile)
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jjohnson
parents:
diff changeset
164 time2 = time.time()
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jjohnson
parents:
diff changeset
165 print >> sys.stderr, "Parsing ensembl ref: %d seconds" % (int(time2-time1))
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jjohnson
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diff changeset
166 except Exception, e:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
167 print >> sys.stderr, "Parsing gene model failed: %s" % e
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
parents:
diff changeset
168 exit(2)
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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diff changeset
169 try:
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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170 parse_input = parse_tabular if options.format == 'tabular' else parse_snpeff_vcf
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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171 for tid,pos1,ref,alt,dp,freq in parse_input():
1
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jjohnson
parents: 0
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172 if options.debug: print >> sys.stderr, "%s\t%d\t%s\t%s\t%d\t%f" % (tid,pos1,ref,alt,dp,freq)
0
9f4ea174ce3d planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit e6aa05bbbee3cc7d98f16354fc41c674f439ff1b-dirty
jjohnson
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diff changeset
173 if not tid:
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jjohnson
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diff changeset
174 continue
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jjohnson
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175 if options.min_depth and dp is not None and dp < options.min_depth:
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jjohnson
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176 continue
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jjohnson
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177 if options.min_freq and freq is not None and freq < options.min_freq:
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178 continue
1
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jjohnson
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179 try:
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180 ## transcript_id, pos, ref, alt, dp, dpr
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181 tx = ens_ref.get_gtf_transcript(tid)
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jjohnson
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182 if not tx and tid.find('.') > 0:
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183 tid = tid.split('.')[0]
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184 tx = ens_ref.get_gtf_transcript(tid)
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185 if not tx:
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186 continue
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jjohnson
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187 if options.debug: print >> sys.stderr, "%s" % (tx)
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188 coding = ens_ref.transcript_is_coding(tid)
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189 if not coding:
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190 continue
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jjohnson
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191 frame_shift = len(ref) != len(alt)
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192 cds = ens_ref.get_cds(tid)
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193 if options.debug or not cds: print >> sys.stderr, "cds:\n%s" % (cds)
a67b4de184c2 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit b62e927469f96a857e880c77530c50c885ad92fc-dirty
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194 pos0 = pos1 - 1 # zero based position
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195 spos = pos0 if tx.gene.strand else pos0 + len(ref) - 1
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196 alt_seq = alt if tx.gene.strand else reverse_complement(alt)
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197 ref_seq = ref if tx.gene.strand else reverse_complement(ref)
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198 cds_pos = ens_ref.genome_to_cds_pos(tid, spos)
2
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199 if cds_pos is None:
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200 continue
1
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jjohnson
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201 if options.debug: print >> sys.stderr, "cds_pos: %s" % (str(cds_pos))
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202 alt_cds = cds[:cds_pos] + alt_seq + cds[cds_pos+len(ref):] if cds_pos+len(ref) < len(cds) else ''
a67b4de184c2 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit b62e927469f96a857e880c77530c50c885ad92fc-dirty
jjohnson
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diff changeset
203 offset = 0
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jjohnson
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204 if tx.gene.strand:
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jjohnson
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205 for i in range(min(len(ref),len(alt))):
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jjohnson
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206 if ref[i] == alt[i]:
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diff changeset
207 offset = i
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jjohnson
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diff changeset
208 else:
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jjohnson
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diff changeset
209 break
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diff changeset
210 else:
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jjohnson
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211 for i in range(-1,-min(len(ref),len(alt)) -1,-1):
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diff changeset
212 if ref[i] == alt[i]:
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diff changeset
213 offset = i
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diff changeset
214 else:
0
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diff changeset
215 break
1
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216 refpep = translate(cds[:len(cds)/3*3])
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217 pep = translate(alt_cds[:len(alt_cds)/3*3])
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diff changeset
218 peplen = len(pep)
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diff changeset
219 aa_pos = (cds_pos + offset) / 3
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diff changeset
220 reported_stop_codon = ''
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221 if aa_pos >= len(pep):
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222 print >> sys.stderr, "aa_pos %d >= peptide length %d : %s %d %s %s" % (aa_pos,len(pep),tid,pos1,ref,alt)
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diff changeset
223 continue
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diff changeset
224 if frame_shift:
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diff changeset
225 #find stop_codons
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226 nstops = 0
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diff changeset
227 stop_codons = []
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228 for i in range(aa_pos,peplen):
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diff changeset
229 if refpep[i] != pep[i]:
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diff changeset
230 aa_pos = i
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jjohnson
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diff changeset
231 break
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diff changeset
232 reported_peptide = pep[aa_pos:]
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233 for i in range(aa_pos,peplen):
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diff changeset
234 if pep[i] == '*':
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diff changeset
235 nstops += 1
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diff changeset
236 stop_codons.append("%s-%s%s" % (alt_cds[i*3-1],alt_cds[i*3:i*3+3],"-%s" % alt_cds[i*3+4] if len(alt_cds) > i*3 else ''))
a67b4de184c2 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit b62e927469f96a857e880c77530c50c885ad92fc-dirty
jjohnson
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diff changeset
237 if nstops > options.readthrough:
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diff changeset
238 reported_peptide = pep[aa_pos:i+1]
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jjohnson
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diff changeset
239 reported_stop_codon = ','.join(stop_codons)
a67b4de184c2 planemo upload for repository https://github.com/jj-umn/galaxytools/tree/master/ensembl_variant_report commit b62e927469f96a857e880c77530c50c885ad92fc-dirty
jjohnson
parents: 0
diff changeset
240 break
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jjohnson
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diff changeset
241 else:
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diff changeset
242 reported_stop_codon = "%s-%s" % (alt_cds[peplen*3-4],alt_cds[peplen*3-3:peplen*3])
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jjohnson
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diff changeset
243 reported_peptide = "%s_%s_%s" % (pep[max(aa_pos-options.leading_aa,0):aa_pos],
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jjohnson
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diff changeset
244 pep[aa_pos],
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jjohnson
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diff changeset
245 pep[aa_pos+1:min(aa_pos+1+options.trailing_aa,len(pep))])
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diff changeset
246 cs_pos = aa_pos * 3
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jjohnson
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diff changeset
247 aa_pos = (cds_pos + offset) / 3
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jjohnson
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diff changeset
248 ref_codon = cds[cs_pos:cs_pos+3]
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jjohnson
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diff changeset
249 ref_aa = translate(ref_codon)
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jjohnson
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250 alt_codon = alt_cds[cs_pos:cs_pos+3]
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251 alt_aa = translate(alt_codon)
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252 gene = tx.gene.names[0]
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253 report_fields = [tx.gene.names[0],
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254 '%s:%d %s' % (tx.gene.contig,pos1,'+' if tx.gene.strand else '-'),
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255 ref_seq,
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256 alt_seq,
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257 "%1.2f" % freq if freq is not None else '',
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258 str(dp),
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259 "%s|%s" % (tx.gene.gene_id,tx.cdna_id),
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260 "%d" % (aa_pos + 1),
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261 "%s%d%s" % (ref_aa,aa_pos + 1,alt_aa),
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262 "%d" % len(pep),
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263 reported_stop_codon,
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264 reported_peptide,
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265 tx.get_transcript_type_name()
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266 ]
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267 if options.debug:
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268 report_fields.append("%d %d %d %d %s %s" % (cds_pos, offset, cs_pos,aa_pos,ref_codon,alt_codon))
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269 outputFile.write('\t'.join(report_fields))
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270 if options.debug:
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271 print >> sys.stderr, "%s %s\n%s\n%s\n%s %s" % (
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272 cds[cs_pos-6:cs_pos], cds[cs_pos:cs_pos+15],
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273 translate(cds[cs_pos-6:cs_pos+15]),
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274 translate(alt_cds[cs_pos-6:cs_pos+15]),
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275 alt_cds[cs_pos-6:cs_pos], alt_cds[cs_pos:cs_pos+15])
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276 outputFile.write('\n')
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277 except Exception, e:
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278 print >> sys.stderr, "failed: %s" % e
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279 print >> sys.stderr, "%s\t%d\t%s\t%s\t%d\t%f\t%s" % (tid,pos1,ref,alt,dp,freq,e)
0
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280 except Exception, e:
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281 print >> sys.stderr, "failed: %s" % e
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282 exit(1)
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283
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284
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285 if __name__ == "__main__" : __main__()
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286