Mercurial > repos > jjohnson > fgbio_call_molecular_consensus_reads
comparison macros.xml @ 0:0ad5327b80cc draft
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
author | jjohnson |
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date | Sun, 21 Feb 2021 23:40:53 +0000 |
parents | |
children | b7795c2f0b81 |
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-1:000000000000 | 0:0ad5327b80cc |
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1 <macros> | |
2 <token name="@TOOL_VERSION@">1.3.0</token> | |
3 <token name="@VERSION_SUFFIX@">0</token> | |
4 <xml name="requirements"> | |
5 <requirements> | |
6 <requirement type="package" version="@TOOL_VERSION@">fgbio</requirement> | |
7 <yield/> | |
8 </requirements> | |
9 </xml> | |
10 <token name="@READ_STRUCTURE_PATTERN@">(([1-9][0-9]*[TBMS])*([+]|[1-9][0-9]*)[TBMS])</token> | |
11 <token name="@READ_STRUCTURES_PATTERN@">@READ_STRUCTURE_PATTERN@(\s@READ_STRUCTURE_PATTERN@)*</token> | |
12 <xml name="read_structures_validator"> | |
13 <validator type="regex" message="">^@READ_STRUCTURES_PATTERN@$</validator> | |
14 </xml> | |
15 <xml name="sam_tag_validator"> | |
16 <validator type="regex" message="">^[A-Za-z][A-Za-z]$</validator> | |
17 </xml> | |
18 <xml name="sam_sort_order"> | |
19 <param argument="--sort-order" type="select" optional="true" label="Sort BAM by"> | |
20 <option value="Coordinate">Coordinate</option> | |
21 <option value="Queryname">Queryname</option> | |
22 <option value="Random">Random</option> | |
23 <option value="RandomQuery">RandomQuery</option> | |
24 </param> | |
25 </xml> | |
26 | |
27 <token name="@READ_STRUCTURES_HELP@"><![CDATA[ | |
28 **Read Structures** | |
29 | |
30 Read structures are made up of <number><operator> pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: | |
31 | |
32 T identifies a template read | |
33 B identifies a sample barcode read | |
34 M identifies a unique molecular index read | |
35 S identifies a set of bases that should be skipped or ignored | |
36 | |
37 The last <number><operator> pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: | |
38 | |
39 :: | |
40 | |
41 --input r1.fq r2.fq i1.fq --read-structures 5M5S+T +T +B | |
42 | |
43 Alternative if you know your reads are of fixed length you could specify: | |
44 | |
45 :: | |
46 | |
47 --input r1.fq r2.fq i1.fq --read-structures 5M5S65T 75T 8B | |
48 | |
49 | |
50 ]]></token> | |
51 <xml name="citations"> | |
52 <citations> | |
53 <yield /> | |
54 </citations> | |
55 </xml> | |
56 </macros> |