Mercurial > repos > jjohnson > fgbio_fastq_to_bam
diff fgbio_fastq_to_bam.xml @ 0:ee774248788f draft
"planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722"
author | jjohnson |
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date | Sun, 21 Feb 2021 23:40:09 +0000 |
parents | |
children | 4635a93ebd91 |
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--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fgbio_fastq_to_bam.xml Sun Feb 21 23:40:09 2021 +0000 @@ -0,0 +1,91 @@ +<tool id="fgbio_fastq_to_bam" name="fgbio FastqToBam" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" python_template_version="3.5"> + <description>Generates an unmapped BAM file from fastq files</description> + <macros> + <import>macros.xml</import> + </macros> + <expand macro="requirements" /> + <version_command>fgbio --version</version_command> + <command detect_errors="exit_code"><![CDATA[ + fgbio FastqToBam + --input + #for $input in $inputs + '$input' + #end for + --sample='$sample' + --library='$library' + #if $read_structures: + --read-structures $read_structures + #end if + --sort='$sort' + --output '$output' + ## optional bam header content + #if $bam_header.umi_tag + --umi-tag='$bam_header.umi_tag' + #end if + #if $bam_header.predicted_insert_size + --predicted-insert-size='$bam_header.predicted_insert_size' + #end if + #if $bam_header.read_group + --read-group='$bam_header.read_group' + #end if + #if $bam_header.description + --description='$bam_header.description' + #end if + #if $bam_header.platform + --platform='$bam_header.platform' + #end if + #if $bam_header.platform_model + --platform-model='$bam_header.platform_model' + #end if + #if $bam_header.platform_model + --platform-model='$bam_header.platform_model' + #end if + #if $bam_header.platform_unit + --platform-unit='$bam_header.platform_unit' + #end if + #if $bam_header.sequencing_center + --sequencing-center='$bam_header.sequencing_center' + #end if + #if $bam_header.comment + --comment='$bam_header.comment' + #end if + ]]></command> + <inputs> + <param name="inputs" type="data" format="fastq" multiple="true" label="Fastq files corresponding to each sequencing read"/> + <param argument="--sample" type="text" value="" label="The name of the sequenced sample"> + </param> + <param argument="--library" type="text" value="" label="The name/ID of the sequenced library"> + </param> + <param argument="--read-structures" type="text" value="" optional="true" label="Read structures, one for each of the FASTQ"> + <expand macro="read_structures_validator" /> + </param> + <param argument="--sort" type="boolean" truevalue="true" falsevalue="false" checked="false" label="Sort bam by queryname" + help="If true, queryname sort the BAM file, otherwise preserve input order."/> + <section name="bam_header" title="BAM Header" expanded="false"> + <param argument="--umi-tag" type="text" value="" optional="true" label="Tag in which to store molecular barcodes/UMIs" help="Default: RX"> + <expand macro="sam_tag_validator" /> + </param> + <param argument="--predicted-insert-size" type="integer" value="" optional="true" label="Predicted median insert size, to insert into the read group header"/> + <param argument="--read-group" type="text" value="" optional="true" label="Read group ID to use in the file header" help="Default: A"/> + <param argument="--description" type="text" value="" optional="true" label="Description of the read group"/> + <param argument="--platform" type="text" value="" optional="true" label="Sequencing Platform" help="Default: illumina"/> + <param argument="--platform-model" type="text" value="" optional="true" label="Platform model to insert into the group header (ex. miseq, hiseq2500, hiseqX)"/> + <param argument="--platform-unit" type="text" value="" optional="true" label="Platform unit (e.g. 'flowcell-barcode.lane.sample-barcode')"/> + <param argument="--sequencing-center" type="text" value="" optional="true" label="The sequencing center from which the data originated"/> + <param argument="--comment" type="text" value="" optional="true" label="Comment to include in the output header"/> + </section> + </inputs> + <outputs> + <data name="output" format="unsorted.bam" /> + </outputs> + <help><![CDATA[ +**fgbio FastqToBam** + +Generates an unmapped BAM (or SAM or CRAM) file from fastq files. Takes in one or more fastq files (optionally gzipped), each representing a different sequencing read (e.g. R1, R2, I1 or I2) and can use a set of read structures to allocate bases in those reads to template reads, sample indices, unique molecular indices, or to designate bases to be skipped over. + +@READ_STRUCTURES_HELP@ + +http://fulcrumgenomics.github.io/fgbio/tools/latest/FastqToBam.html + ]]></help> + <expand macro="citations" /> +</tool>