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view fgbio_fastq_to_bam.xml @ 2:6137ff37bea1 draft default tip
"planemo upload commit 77a5370a0978b5332bb3a9f063588a52a468ea08"
author | jjohnson |
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date | Thu, 19 Aug 2021 15:11:08 +0000 |
parents | 4635a93ebd91 |
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<tool id="fgbio_fastq_to_bam" name="fgbio FastqToBam" version="@TOOL_VERSION@+galaxy@VERSION_SUFFIX@" python_template_version="3.5"> <description>Generates an unmapped BAM file from fastq files</description> <macros> <import>macros.xml</import> </macros> <expand macro="requirements" /> <version_command>fgbio --version</version_command> <command detect_errors="exit_code"><![CDATA[ @LINK_FASTQ_INPUTS@ fgbio FastqToBam @FASTQ_INPUTS@ --sample='$sample' --library='$library' --sort='$sort' --output '$output' ## optional bam header content #if $bam_header.umi_tag --umi-tag='$bam_header.umi_tag' #end if #if $bam_header.predicted_insert_size --predicted-insert-size='$bam_header.predicted_insert_size' #end if #if $bam_header.read_group --read-group='$bam_header.read_group' #end if #if $bam_header.description --description='$bam_header.description' #end if #if $bam_header.platform --platform='$bam_header.platform' #end if #if $bam_header.platform_model --platform-model='$bam_header.platform_model' #end if #if $bam_header.platform_model --platform-model='$bam_header.platform_model' #end if #if $bam_header.platform_unit --platform-unit='$bam_header.platform_unit' #end if #if $bam_header.sequencing_center --sequencing-center='$bam_header.sequencing_center' #end if #if $bam_header.comment --comment='$bam_header.comment' #end if ]]></command> <inputs> <expand macro="fastq_inputs"/> <param argument="--sample" type="text" value="" label="The name of the sequenced sample"> <validator type="empty_field"/> </param> <param argument="--library" type="text" value="" label="The name/ID of the sequenced library"> <validator type="empty_field"/> </param> <param argument="--sort" type="boolean" truevalue="true" falsevalue="false" checked="false" label="Sort bam by queryname" help="If true, queryname sort the BAM file, otherwise preserve input order."/> <section name="bam_header" title="BAM Header" expanded="false"> <param argument="--umi-tag" type="text" value="" optional="true" label="Tag in which to store molecular barcodes/UMIs" help="Default: RX"> <expand macro="sam_tag_validator" /> </param> <param argument="--predicted-insert-size" type="integer" value="" optional="true" label="Predicted median insert size, to insert into the read group header"/> <param argument="--read-group" type="text" value="" optional="true" label="Read group ID to use in the file header" help="Default: A"/> <param argument="--description" type="text" value="" optional="true" label="Description of the read group"/> <param argument="--platform" type="text" value="" optional="true" label="Sequencing Platform" help="Default: illumina"/> <param argument="--platform-model" type="text" value="" optional="true" label="Platform model to insert into the group header (ex. miseq, hiseq2500, hiseqX)"/> <param argument="--platform-unit" type="text" value="" optional="true" label="Platform unit (e.g. 'flowcell-barcode.lane.sample-barcode')"/> <param argument="--sequencing-center" type="text" value="" optional="true" label="The sequencing center from which the data originated"/> <param argument="--comment" type="text" value="" optional="true" label="Comment to include in the output header"/> </section> </inputs> <outputs> <data name="output" format="bam" > <expand macro="sort_order_change_format" /> </data> </outputs> <help><![CDATA[ **fgbio FastqToBam** Generates an unmapped BAM (or SAM or CRAM) file from fastq files. Takes in one or more fastq files (optionally gzipped), each representing a different sequencing read (e.g. R1, R2, I1 or I2) and can use a set of read structures to allocate bases in those reads to template reads, sample indices, unique molecular indices, or to designate bases to be skipped over. @READ_STRUCTURES_HELP@ http://fulcrumgenomics.github.io/fgbio/tools/latest/FastqToBam.html ]]></help> <expand macro="citations" /> </tool>