# HG changeset patch # User jjohnson # Date 1613950876 0 # Node ID 286e35cf3f1c43139211ee5d0b9c4e1401b3a5a0 "planemo upload commit 61f6c8e7f32f170ad7e66e46dd74e8c5d361a722" diff -r 000000000000 -r 286e35cf3f1c fgbio_sort_bam.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/fgbio_sort_bam.xml Sun Feb 21 23:41:16 2021 +0000 @@ -0,0 +1,34 @@ + + Sorts a SAM or BAM file + + macros.xml + + + fgbio --version + + + + + + + + + + + diff -r 000000000000 -r 286e35cf3f1c macros.xml --- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/macros.xml Sun Feb 21 23:41:16 2021 +0000 @@ -0,0 +1,56 @@ + + 1.3.0 + 0 + + + fgbio + + + + (([1-9][0-9]*[TBMS])*([+]|[1-9][0-9]*)[TBMS]) + @READ_STRUCTURE_PATTERN@(\s@READ_STRUCTURE_PATTERN@)* + + ^@READ_STRUCTURES_PATTERN@$ + + + ^[A-Za-z][A-Za-z]$ + + + + + + + + + + + pairs much like the CIGAR string in BAM files. Four kinds of operators are recognized: + + T identifies a template read + B identifies a sample barcode read + M identifies a unique molecular index read + S identifies a set of bases that should be skipped or ignored + +The last pair may be specified using a + sign instead of number to denote “all remaining bases”. This is useful if, e.g., fastqs have been trimmed and contain reads of varying length. For example to convert a paired-end run with an index read and where the first 5 bases of R1 are a UMI and the second five bases are monotemplate you might specify: + +:: + + --input r1.fq r2.fq i1.fq --read-structures 5M5S+T +T +B + +Alternative if you know your reads are of fixed length you could specify: + +:: + + --input r1.fq r2.fq i1.fq --read-structures 5M5S65T 75T 8B + + +]]> + + + + + +