<tool id="mothur_amova" name="Amova" version="1.19.0">
 <description>Analysis of molecular variance</description>
 <command interpreter="python">
  mothur_wrapper.py 
  --cmd='amova'
  --result='^mothur.\S+\.logfile$:'$logfile,'^\S+\.amova$:'$amova
  --outputdir='$logfile.extra_files_path'
  --phylip=$dist
  --design=$design
  #if int($iters.__str__) > 0:
   --iters=$iters
  #end if
  #if float($alpha.__str__) > 0.0:
   --alpha=$alpha
  #end if
 </command>
 <inputs>
  <param name="dist" type="data" format="lower.dist,square.dist" label="phylip - Distance Matrix"/>
  <param name="design" type="data" format="tabular" label="design - assign groups to new grouping"
         help="design has 2 TAB-separated columns: group and grouping (Tool: Make_Design) "/>
  <param name="alpha" type="float" optional="true" value="0.05" label="alpha - acceptable stopping precision (default 0.05)"/>
  <param name="iters" type="integer" value="1000" label="iters - Number of random configuration to try (default 1000)"/>
 </inputs>
 <outputs>
  <data format="html" name="logfile" label="${tool.name} on ${on_string}: logfile" />
  <data format="tabular" name="amova" label="${tool.name} on ${on_string}: amova"/>
 </outputs>
 <requirements>
  <requirement type="package" version="1.27">mothur</requirement>
 </requirements>
 <tests>
 </tests>
 <help>
**Mothur Overview**

Mothur_, initiated by Dr. Patrick Schloss and his software development team
in the Department of Microbiology and Immunology at The University of Michigan,
provides bioinformatics for the microbial ecology community.

.. _Mothur: http://www.mothur.org/wiki/Main_Page

**Command Documenation**

The amova_ command calculates the analysis of molecular variance from a phylip_distance_matrix_, a nonparametric analog of traditional analysis of variance. This method is widely used in population genetics to test the hypothesis that genetic diversity within two populations is not significantly different from that which would result from pooling the two populations.

A design file partitions a list of names into groups.  It is a tab-delimited file with 2 columns: name and group, e.g. :
	=======	=======
	duck	bird
	cow	mammal
	pig	mammal
	goose	bird
	cobra	reptile
	=======	=======

The Make_Design tool can construct a design file from a Mothur dataset that contains group names.


.. _phylip_distance_matrix: http://www.mothur.org/wiki/Phylip-formatted_distance_matrix
.. _amova: http://www.mothur.org/wiki/Amova


 </help>
</tool>