Mercurial > repos > jjohnson > qiime
diff align_seqs.xml @ 0:e5c3175506b7 default tip
Initial tool configs for qiime, most need work.
author | Jim Johnson <jj@umn.edu> |
---|---|
date | Sun, 17 Jul 2011 10:30:11 -0500 |
parents | |
children |
line wrap: on
line diff
--- /dev/null Thu Jan 01 00:00:00 1970 +0000 +++ b/align_seqs.xml Sun Jul 17 10:30:11 2011 -0500 @@ -0,0 +1,127 @@ +<tool id="align_seqs" name="align_seqs" version="1.2.0"> + <description>Align sequences using a variety of alignment methods</description> + <requirements> + <requirement type="binary">align_seqs.py</requirement> + </requirements> + <command interpreter="python"> + qiime_wrapper.py + --galaxy_outputdir='$log.extra_files_path' + --galaxy_datasets='^\S+_aligned\.\S+$:'$aligned_fasta,'^\S+_log\.txt$:'$log,'^\S+_failures\.fasta$:'$failures + align_seqs.py + --input_fasta_fp=$input_fasta_fp + --alignment_method=$align.alignment_method + #if $align.alignment_method == 'pynast': + --template_fp=$align.alignment.template_fp + --pairwise_alignment_method=$align.pairwise_alignment_method + --min_length=$align.min_length + --min_percent_id=$align.min_percent_id + --blast_db=$align.blast_db + #elif $align.alignment_method == 'infernal': + --template_fp=$align.alignment.template_fp + #elif $align.alignment_method == 'clustalw': + #echo '' + #elif $align.alignment_method == 'muscle': + #echo '' + #elif $align.alignment_method == 'mafft': + #echo '' + #end if + + --output_dir='$log.extra_files_path' + </command> + <inputs> + <param name="input_fasta_fp" type="data" format="fasta" label="input_fasta_fp" + help="path to the input fasta file [REQUIRED]"/> + + <conditional name="align"> + <param name="alignment_method" type="select" label="alignment_method" + help="Method for aligning sequences. Valid choices are: pynast, infernal, clustalw, muscle, infernal, mafft [default: pynast]"> + <option value="pynast" selected="true">pynast</option> + <option value="infernal">infernal</option> + <option value="clustalw">clustalw</option> + <option value="muscle">muscle</option> + <option value="mafft">mafft</option> + </param> + + <when value="pynast"> + <conditional name="alignment"> + <param name="source" type="select" label="Select Template from" help=""> + <option value="hist">History</option> + <option value="ref">Cached Reference</option> + </param> + <when value="ref"> + <param name="template_fp" type="select" label="template - Select an alignment database " help=""> + <options from_file="mothur_aligndb.loc"> + <column name="name" index="0" /> + <column name="value" index="1" /> + </options> + </param> + </when> + <when value="hist"> + <param name="template_fp" type="data" format="txt" label="template_fp" + help="Filepath for template against [REQUIRED if for alignment_method pynast or infernal]"/> + </when> + </conditional> + <param name="pairwise_alignment_method" type="select" label="pairwise_alignment_method" + help="method for performing pairwise alignment in PyNAST. Valid choices are muscle, pair_hmm, clustal, blast, uclust, mafft [default: uclust]"> + <option value="muscle">muscle</option> + <option value="pair_hmm">pair_hmm</option> + <option value="clustal">clustal</option> + <option value="blast">blast</option> + <option value="uclust" selected="true">uclust</option> + <option value="mafft">mafft</option> + </param> + <param name="min_length" type="integer" value="150" label="min_length" + help="Minimum sequence length to include in alignment [default: 150]"/> + <param name="min_percent_id" type="float" value="0.75" label="min_percent_id" + help="Minimum percent sequence identity to closest blast hit to include sequence in alignment [default: 0.75]"/> + <param name="blast_db" type="text" label="blast_db" optional="true" + help="Database to blast against when -m pynast [default: created on-the-fly from template_alignment]"/> + </when> <!-- pynast --> + + <when value="infernal"> + <!-- Template alignment must be in Stockholm format with corresponding secondary structure annotation when using InfernalAligner. --> + <conditional name="alignment"> + <param name="source" type="select" label="Select Template from" help=""> + <option value="hist">History</option> + <option value="ref">Cached Reference</option> + </param> + <when value="ref"> + <param name="template_fp" type="select" label="template - Select an alignment database " help=""> + <options from_file="mothur_aligndb.loc"> + <column name="name" index="0" /> + <column name="value" index="1" /> + </options> + </param> + </when> + <when value="hist"> + <param name="template_fp" type="data" format="txt" label="template_fp" + help="Filepath for template against [REQUIRED if for alignment_method pynast or infernal]"/> + </when> + </conditional> + </when> <!-- infernal --> + + <when value="clustalw"> + </when> <!-- clustalw --> + <when value="muscle"> + </when> <!-- muscle --> + <when value="mafft"> + </when> <!-- mafft --> + + </conditional> <!-- align --> + + + </inputs> + <outputs> + <data format="txt" name="log" label="${tool.name} on ${on_string}: log" /> + <data format="fasta" name="aligned_fasta" label="${tool.name} on ${on_string}: aligned fasta" /> + <data format="fasta" name="failures" label="${tool.name} on ${on_string}: failures" > + <filter>align['alignment_method'] == 'pynast'</filter> + </data> + </outputs> + <tests> + </tests> + <help> + + </help> +</tool> +