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author | john-mccallum |
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date | Thu, 06 Sep 2012 23:05:22 -0400 |
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<?xml version="1.0"?> <tool id="dnaclust_1" name="dnaclust"> <description>Cluster sequences into OTUs using DNAclust </description> <command> dnaclust -s $similarity -i $inputFastaFile > $outputfile </command> <inputs> <param format="fasta" name="inputFastaFile" type="data" label="Fasta input file"/> <param name="similarity" size="10" type="text" value="0.99" label="similarity between center and cluster sequences" /> </inputs> <outputs> <data format="tabular" name="outputfile" /> </outputs> <help> .. class:: infomark **TIP** see the DNACLUST documentation at http://dnaclust.sourceforge.net/ Each line will contain the ids of the sequences in each cluster, and the first id of each line is the cluster representative. Example: To cluster a set of 16S rRNA fragments at 0.98 similarity use: ./dnaclust file.fasta -l -s 0.98 > clusters You can optionally specify a k-mer length for the filter. The longer k-mers use more memory. Also the filter will be more specific with longer k-mers. The default log_4(median length) should be good for mo\ st cases. </help> </tool>